Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,020,956 A→G intergenic (‑37/‑182) sulA ← / → sxy SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,020,9560AG98.6% 219.5 / NA 69intergenic (‑37/‑182)sulA/sxySOS cell division inhibitor/CRP‑S‑dependent promoter expression factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (41/27);  minor base C (0/1);  total (41/28)
Fisher's exact test for biased strand distribution p-value = 4.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGT  >  NC_000913/1020817‑1021093
                                                                                                                                           |                                                                                                                                         
cACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTGGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcag                                                                                                                                        <  1:880720/143‑1 (MQ=255)
   tGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATgtgcagtac                                                                                                                                     >  1:697245/1‑143 (MQ=255)
      tAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATc                                                                                                                                  <  2:488010/143‑1 (MQ=255)
      tAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCCGAAGTGTACGTATTCGATCCAGCCCCTGTGAGCTACTGTATGTATGTGCAGTACATc                                                                                                                                  <  1:445386/143‑1 (MQ=255)
       aaGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATcc                                                                                                                                 >  2:879222/1‑143 (MQ=255)
         gCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAg                                                                                                                               <  1:127661/143‑1 (MQ=255)
          cccGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGCTACTGTATGGATGTGCAGTACCTCCAGt                                                                                                                              >  2:1169607/1‑143 (MQ=255)
            cGGCTGTAGTGTGTTCCGTCGAGACACGCGCACTTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGa                                                                                                                            <  1:136727/143‑1 (MQ=255)
                  tAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGCTGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGTGTTACTGTATGGATGTGGAGTACATCCAGTGccaacca                                                                                                                      >  2:217510/1‑141 (MQ=255)
                  tAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGAcaacaa                                                                                                                      >  1:168726/1‑143 (MQ=255)
                         ttCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTTATGTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCaa                                                                                                               >  1:1113397/1‑143 (MQ=255)
                          tgcggagagACTCGAGCAATTTGTCTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAGTCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAAc                                                                                                              <  2:685534/138‑1 (MQ=255)
                                    aacGCGCAATTTTAGTTACTGCAGATTAGAACTACTAATAACTATTTGCATAGCCTTAATTGTACATAATAATTCCAGCCCCTGTGACTTACTGTATTGATGTGCAGTACATCCAGTGACAACAAATATCAACCCTATTTTCg                                                                                                    <  2:181363/142‑1 (MQ=255)
                                         gcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAag                                                                                               >  1:873166/1‑143 (MQ=255)
                                         gcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATCTTCGGAAag                                                                                               >  1:993695/1‑143 (MQ=255)
                                               ttACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTc                                                                                         <  1:1143458/143‑1 (MQ=255)
                                               ttACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTc                                                                                         <  1:179330/143‑1 (MQ=255)
                                                 aCTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCCGTGACACCAAAGATCAACCCTACTCTCCCAAAGAGCCTcca                                                                                       >  2:1169137/1‑141 (MQ=255)
                                                  cTTGCTGCGGATGAGAACGACGAAGAACGCTGTGAAAAGAAAGATATTTACAGAATCAATCCAGCCCCTGTGAGTTACTGTATGGCTGTGCAGTACATCCACTCACAACAAAGATCAACCCTATTGTCGCAAACAGCCTCGCa                                                                                      >  2:252576/1‑143 (MQ=255)
                                                   ttGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCaa                                                                                     >  2:92362/1‑143 (MQ=255)
                                                    tgctgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCaaa                                                                                    <  1:139732/143‑1 (MQ=255)
                                                    tgcctcGGATGCGAACGACGAATAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCaaa                                                                                    <  2:959903/138‑1 (MQ=255)
                                                    cgctgcGGATGAGAACCACCAAGAACGATGTGCATCGCCTGCAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCaaa                                                                                    <  1:177939/142‑1 (MQ=255)
                                                      ctgcGGATGAGAACGATGAAGAACGATGTGCATAGCCTGAAGTGGACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCGTCGCAAAtt                                                                                  >  2:551104/1‑143 (MQ=255)
                                                       tgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAttt                                                                                 >  1:356481/1‑143 (MQ=255)
                                                       tgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAATGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATACAGTGACAACAAAGATAAACCGTAATATAGAAAAGAGCCTAGCaaaata                                                                                 >  2:620831/1‑140 (MQ=255)
                                                        gcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTCCTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAtttt                                                                                <  1:501863/143‑1 (MQ=255)
                                                           gATTAGTACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTc                                                                             <  1:482934/143‑1 (MQ=255)
                                                            aTGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGACCCTGTGCGTAACTGTATGGATGTGCAGTACATCCAGTGTCACCACAGATCAACCCTATTTTCTGAATGAGCCTCGCACACTTTGTcc                                                                            >  1:773641/1‑142 (MQ=255)
                                                               agaACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTg                                                                         <  1:975674/143‑1 (MQ=255)
                                                               agaACGACGAAGAACGATGTGCATAGCCTGAAGTGTACAGAATCAATCCAGCCCCTGTGAGGTACTGTATGGATGTGCAGTACACCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTg                                                                         <  1:1110885/143‑1 (MQ=255)
                                                                 aaCGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGt                                                                       >  1:394914/1‑143 (MQ=255)
                                                                 aaCGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATACAGCACCTGTGAGATAATGTATGGATGAGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGt                                                                       >  1:837611/1‑143 (MQ=255)
                                                                    gacgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGAc                                                                    >  2:1008747/1‑143 (MQ=255)
                                                                      cgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGGAGTACAGCCAGGGACAACAAAGATCAACCGTATTTTGGGAAAGAGAGTGGCAAATTTTGTCGGTGGTGACgg                                                                  >  1:133955/1‑143 (MQ=255)
                                                                      cgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACgg                                                                  >  2:143542/1‑143 (MQ=255)
                                                                      cgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACgg                                                                  >  2:929142/1‑143 (MQ=255)
                                                                       gaagaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAGATTTTGTCGTCGGTGCCggg                                                                 >  1:677298/1‑143 (MQ=255)
                                                                         agaaCGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGaa                                                               >  1:230578/1‑143 (MQ=255)
                                                                                tgtgCATAGGCGGGAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATGAACGCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATaa                                                        <  2:328511/143‑1 (MQ=255)
                                                                                     aTAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACCTAAAttca                                                   >  1:1024/1‑141 (MQ=255)
                                                                                     aTAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTaa                                                   >  2:1074671/1‑143 (MQ=255)
                                                                                      tAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAAt                                                  <  1:916618/143‑1 (MQ=255)
                                                                                          cTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATCAATTAATCTTg                                              >  2:1022580/1‑143 (MQ=255)
                                                                                          cTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTg                                              <  1:1118582/143‑1 (MQ=255)
                                                                                            gAAGGGTACATAATCAATCCGGCCCCTGTGAGTTACTGTATGGATGTGCAGTACAGCCAGTGACAACAAAGATCAACCCTATTGTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGcc                                            <  1:465026/143‑1 (MQ=255)
                                                                                             aaGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGATGGTGACGGGAAAACATAAATTAATCTTGccc                                           >  2:27508/1‑143 (MQ=255)
                                                                                                tgtACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCtt                                        >  1:476737/1‑143 (MQ=255)
                                                                                                  tACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTaa                                      <  1:648536/143‑1 (MQ=255)
                                                                                                     aTAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTGGAGTACATCCAGTGACAACAAAGATCAACCCTGTTTTCGGAAAGAGCATAGCAAATTTTGTCGTTGGTGACGGGAAAAAATCAATTAATCTTGCCCCTTAAGaa                                   >  2:713309/1‑143 (MQ=255)
                                                                                                       aatcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATa                                 >  2:244462/1‑143 (MQ=255)
                                                                                                        atcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGACAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTAGCCCCTGAAGAATaa                                >  1:95712/1‑143 (MQ=255)
                                                                                                         tcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAg                               >  2:396997/1‑143 (MQ=255)
                                                                                                         tcaatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAg                               <  1:1033519/143‑1 (MQ=255)
                                                                                                           aatcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGtt                             >  2:677948/1‑143 (MQ=255)
                                                                                                             tcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGc                           >  1:792604/1‑143 (MQ=255)
                                                                                                             tcCAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGc                           <  2:230578/143‑1 (MQ=255)
                                                                                                               cAGCCCCTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTGAAGAATAGGTGGCCt                         >  1:363404/1‑143 (MQ=255)
                                                                                                                   cccTGTGAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTAttt                     <  2:170348/143‑1 (MQ=255)
                                                                                                                       gtgagtgaCTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGt                 <  2:1113397/143‑1 (MQ=255)
                                                                                                                        tgAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATGAGTTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTa                >  1:410469/1‑143 (MQ=255)
                                                                                                                        tgAGTTACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTa                >  1:369696/1‑143 (MQ=255)
                                                                                                                            ttACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTa            >  1:945759/1‑143 (MQ=255)
                                                                                                                            ttACTGTATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTa            >  1:533348/1‑143 (MQ=255)
                                                                                                                               ctctATGGATGTCCAGTACATCCAGTGACAACAAAGCTCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGCAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACg         <  2:55334/140‑1 (MQ=255)
                                                                                                                               cTGTAGGGATCGGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAATAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACg         <  1:723912/143‑1 (MQ=255)
                                                                                                                                  tATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTTGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAAAGGAt      >  1:521100/1‑143 (MQ=255)
                                                                                                                                  tATGGATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTACCTATTTTCGTAGTTAACGGAt      >  1:1126548/1‑143 (MQ=255)
                                                                                                                                  tATGGATGTGCAGTACAGCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGAt      <  1:503818/143‑1 (MQ=255)
                                                                                                                                      gATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGt  <  1:378103/143‑1 (MQ=255)
                                                                                                                                      gATGTGCAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGt  >  1:1142296/1‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                         
CACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCCTATTTTCGTAGTTAACGGATCCGT  >  NC_000913/1020817‑1021093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: