breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsEvo04gndEvo03EP_S19_L001_R1_001.good.fq809,394210,547,738100.0%260.1 bases289 bases99.3%
errorsEvo04gndEvo03EP_S19_L001_R2_001.good.fq809,394210,662,646100.0%260.3 bases289 bases97.0%
total1,618,788421,210,384100.0%260.2 bases289 bases98.2%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0009134,641,65292.14.5100.0%Escherichia coli str. K-12 substr. MG1655, complete genome.
total4,641,652100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 1000009601
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 3
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 500039
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.005

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0009130.87920

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.3.4.1
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input20:47:18 27 Feb 202020:47:44 27 Feb 202026 seconds
Read alignment to reference genome20:47:44 27 Feb 202020:54:10 27 Feb 20206 minutes 26 seconds
Preprocessing alignments for candidate junction identification20:54:10 27 Feb 202020:54:38 27 Feb 202028 seconds
Preliminary analysis of coverage distribution20:54:38 27 Feb 202020:55:54 27 Feb 20201 minute 16 seconds
Identifying junction candidates20:55:54 27 Feb 202020:56:18 27 Feb 202024 seconds
Re-alignment to junction candidates20:56:18 27 Feb 202020:57:56 27 Feb 20201 minute 38 seconds
Resolving best read alignments20:57:56 27 Feb 202020:58:40 27 Feb 202044 seconds
Creating BAM files20:58:40 27 Feb 202020:59:46 27 Feb 20201 minute 6 seconds
Tabulating error counts20:59:46 27 Feb 202021:00:27 27 Feb 202041 seconds
Re-calibrating base error rates21:00:27 27 Feb 202021:00:28 27 Feb 20201 second
Examining read alignment evidence21:00:28 27 Feb 202021:24:47 27 Feb 202024 minutes 19 seconds
Polymorphism statistics21:24:47 27 Feb 202021:24:48 27 Feb 20201 second
Output21:24:48 27 Feb 202021:25:07 27 Feb 202019 seconds
Total 37 minutes 49 seconds