Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 4,242,636 | Δ1 bp | coding (881/891 nt) | malG ← | maltose transporter subunit |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,242,636 | 0 | A | . | 97.3% | 148.9 / ‑3.2 | 37 | coding (881/891 nt) | malG | maltose transporter subunit |
Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (20/16); minor base C (1/0); total (21/16) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTAGTTTTGCGGGTTGAGGTATTGC > NC_000913/4242501‑4242767 | ccGGCGAACGATTCAGATCGCAGCCGAACGCACATAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCt < 1:194712/143‑1 (MQ=255) ggCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACACCTTCACCCCTTC‑CACCACCTGc > 1:208325/1‑143 (MQ=255) cGATTCAGATTGCAGACGCAAGAAAAAAAGGCGCTCCGTGGAGCCCCGAATAACAGTCACAAGTTGGGATACCGTAAGGTGAGGGTGCAGCGGCACAACCTGGGCAGAAACACACCGTTCGCCTTTC‑CACCCCCTGCCGTCAg > 2:91107/1‑143 (MQ=255) aTTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGACGCTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGc < 1:103192/143‑1 (MQ=255) ttCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCAGGACAGTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCGGGct > 1:412482/1‑142 (MQ=255) tCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCg > 1:666768/1‑143 (MQ=255) aGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTACCCTTTC‑CACCACCTGCCGTCAGGCCGtt > 1:259797/1‑143 (MQ=255) gCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTCCGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTACCGACAGACCGTTGAcca > 1:685499/1‑143 (MQ=255) aGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGAccagc > 2:328420/1‑143 (MQ=255) gaaagaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCCGAACCACACCTGTCACC‑GTCCCACCACCTGCCGTCACGCCGTTGACcagccagc > 2:564842/1‑143 (MQ=255) aagaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGt > 1:111310/1‑143 (MQ=255) aagaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAAAGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCGGTTGACCAGCCAGCGt > 1:32625/1‑143 (MQ=255) agaaaAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTGTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGtt > 1:233081/1‑143 (MQ=255) aaaaaaaGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCGCGCGTTGa > 1:674053/1‑143 (MQ=255) aaaaaaaGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGa < 1:215239/143‑1 (MQ=255) aaaaaGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGc < 2:142936/143‑1 (MQ=255) tCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCaagaag > 1:434854/1‑143 (MQ=255) gTGGACCTCCGAATAACATGCACAAGAGGAAATAACGTAACTTGAGGGTTCAGCGGCATAACAGTGGCAGAACAACCTCTCTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACg < 2:147308/143‑1 (MQ=255) gcgcGGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGt < 2:203949/143‑1 (MQ=255) cgcCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGTAAGAAGACGATGGTg < 1:469324/143‑1 (MQ=255) ccGAATAACAGTCTAGTGTGTGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGAt < 2:470382/143‑1 (MQ=255) ccGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGAt > 1:345389/1‑143 (MQ=255) gAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCg < 1:702238/143‑1 (MQ=255) tAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTGTAACCGTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTa < 2:461665/143‑1 (MQ=255) gTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCa < 1:595439/143‑1 (MQ=255) acaAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCGTTC‑CACCACCTGCCGTCGGGGCGTGGACCAGCCAGCGTTGAGCCAGCGCGAAGACGATGTTGCTCGGTAATGCAGAc > 1:333668/1‑143 (MQ=255) aaGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCGGGCATCACGGCAGCggcggc > 1:616520/1‑143 (MQ=255) gtaGGGGGCAGCGGCGTACCGTTGGCAGAGCAACATCTTTAACCTTTC‑CGCCACCGGCCGTCAGGCCGTCGTCCAGCCAGCGTTGATCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAg < 2:314357/141‑1 (MQ=255) ggCAGCGGCATAACCTTGGCAGCACGCCATCCTTAACCTTTC‑CACCCCCTGCCGTCAGGCCGTTGACCACCCAGCTTTGAGCCAGCAAGCAGCCGATGGTGATCTGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCAccc < 2:599084/142‑1 (MQ=255) cATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGGCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAGTGAAGACATCGTGGCAGCGGCGGCAAAGTCACCGCACAGGtg > 1:270702/1‑142 (MQ=255) cATAACATTGGCAGAACAACATCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTa < 2:62609/143‑1 (MQ=255) ttGGCAGAACTACATCTTTAACCTTTC‑CAACACCCGCCGTCAGGTACCAGAGGTGCCGTGTTTGTGCCCGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTAGTTTTGc < 2:153055/143‑1 (MQ=255) acaacaTCTTTACCCTTTC‑CACCGCCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTAGTTTTGCggcctaag < 2:126625/143‑7 (MQ=255) caacaTCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGGCCACCCGCAGGGAGTTTTGGGGGGTgggg > 2:577519/1‑143 (MQ=255) caacaTCTGTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGGAAAGTCACCCCACAGGCGGGCTTGCGGGATGAgg > 2:719934/1‑143 (MQ=255) aacaTCTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTAGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCCGCAAAGCCCCCCCAAAGGTAGATTTGCGGGCTCAgga > 2:64887/1‑142 (MQ=255) cTTTAACCTTTC‑CACCACCTGCCGTCAGGCCGTTAACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCGGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTAGTTTTGGGGTTGGAgggttggc > 1:225171/1‑137 (MQ=255) | CCGGCGAACGATTCAGATTGCAGACGAAAGAAAAAAAGGCGCTCCGTGGAGCGCCGAATAACAGTCACAAGTTGGGATAACGTAAGTTGAGGGTGCAGCGGCATAACATTGGCAGAACAACATCTTTAACCTTTCACACCACCTGCCGTCAGGCCGTTGACCAGCCAGCGTTGAGCCAGCAAGAAGACGATGGTGATCGGTAATGCAGACATCACGGCAGCGGCGGCAAAGTCACCCCACAGGTAGTTTTGCGGGTTGAGGTATTGC > NC_000913/4242501‑4242767 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |