| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 699,471 | Δ1 bp | intergenic (‑294/+103) | asnB ← / ← umpH | asparagine synthetase B/UMP phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 699,468 | 0 | A | . | 97.9% | 182.2 / ‑3.4 | 48 | intergenic (‑291/+106) | asnB/umpH | asparagine synthetase B/UMP phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (21/26); minor base G (1/0); total (22/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACG > NC_000913/699327‑699602 | aGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGCACGCCTTATCCGGCCTACAAAAGCAGGaa > 1:415098/1‑143 (MQ=255) atatTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATGTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGaaattca < 1:45523/143‑5 (MQ=255) tatttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCaa < 1:605081/143‑1 (MQ=255) gagttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCaa < 1:58476/140‑1 (MQ=255) atttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAat > 1:323453/1‑143 (MQ=255) atttattGAGGAATCGATAATTGTTAGCGGGTTTTATGGACTGTTTCTATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAat < 2:628073/143‑1 (MQ=25) tgatgcATCGATAAGTTGTAGCGGGTTTGATTGAATGTTTATATTTGACGGGGGCCAAATTGCTGACAAAGTTCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTg < 1:570749/143‑1 (MQ=25) tgatgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAACGCATG‑AAATTCAATATATTg > 1:541462/1‑143 (MQ=255) atgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCa > 1:181712/1‑143 (MQ=255) atgaATCGATAATTTTTAGCGGGTTTTATTGAATGTGCATAGGGGACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCa > 1:693951/1‑143 (MQ=25) tgaATCGATAATTTGTAGCGGGTTTTAGTGAATGTTTATCTTTTACGGGGGCCAAATTGCTGACATAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAg < 2:303238/143‑1 (MQ=25) tCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGAAGGAGc > 2:554559/1‑143 (MQ=255) aTAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGAGCGATTTGTTCATGCCGGATGCGGCGTGAACGACTTATCCGGCCTACAAAAGCACGGAGA‑TCAAGATATTGCAGGAGCTGc > 2:472203/1‑143 (MQ=25) tttAGCGGGTTTTATTGAGTGTTTATGTTTTATGGGGGCCACATTGCGGACAAATTGCGATTTGTTCATGCCTTATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGc < 2:627775/143‑1 (MQ=25) tttAGCGGGTTTTATTGAATGTTTATATGGTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGc < 1:612787/143‑1 (MQ=255) ttgggCGGGTTTGATTGAGTGTTGGTATTTGACGGGGGCGAAGTTGCTGACAGAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACAGAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGc < 2:27034/139‑1 (MQ=18) cGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGCGCCGCGTAGGCCTGAt > 2:540799/1‑143 (MQ=255) tATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTa < 1:297039/143‑1 (MQ=255) ttGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAgc < 1:429898/143‑1 (MQ=255) gAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑ACATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAgcgc > 1:474566/1‑143 (MQ=255) aaTGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGAGAAGAGTAGTGCa > 2:413990/1‑143 (MQ=255) tGTTTAGATTTTACGGGGGTCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGGCTACAAAAGCATG‑AAATTCAATATATTGCATGAGCTGCGTAGGCCTGATAAGCGTAGCGCATc < 2:20546/143‑1 (MQ=18) ttATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCTCATCAgg < 2:484113/143‑1 (MQ=255) atTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGt < 2:534542/143‑1 (MQ=255) cGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCg < 2:181712/143‑1 (MQ=255) gggCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGttt < 1:337592/143‑1 (MQ=255) gggCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGttt < 1:306086/143‑1 (MQ=255) ggCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTg < 2:228417/143‑1 (MQ=255) gCCAAATTGCTGACGAAGTGGGATTTGTTCGTGTCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATGGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGt < 2:40520/143‑1 (MQ=18) ccAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCAACAGGCAGGTTGACgctggac > 2:562641/1‑137 (MQ=18) tGCTGATAGAGTGCGATTTGTTAAGGCCGGACGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCaga < 2:204406/143‑1 (MQ=18) tGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGcc > 1:73195/1‑143 (MQ=255) aCAAAGTGCGGTTGGTTCAGGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCaa < 1:299925/143‑1 (MQ=18) aaaGGGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAAcc < 2:272884/143‑1 (MQ=255) aTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTgg > 1:149828/1‑143 (MQ=255) tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGt < 1:320144/143‑1 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTg > 1:346222/1‑143 (MQ=255) ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAAGTCAAAATATTGCAGAAGCTGCGTAAGACTGATAAGCATAGCGCATCAGGCAGTTTGGCGATAGAAAACAGAGCCAACCACGTCCACAAACGTGGGTg > 2:269353/1‑143 (MQ=17) tGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTAtt < 1:554559/143‑1 (MQ=255) tGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGATGCAAtt > 1:397176/1‑143 (MQ=255) ggcgTGAACGCCTTATCCGGCCTACAAACGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAg < 1:415306/143‑1 (MQ=255) tGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATaa > 2:599588/1‑143 (MQ=255) tGAACGCCTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATaa > 1:519397/1‑143 (MQ=255) gCCTTATCCGGCCTACACAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCg > 2:90830/1‑143 (MQ=255) cTTATCCGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCACAGACGTGGTTGCTCTTCAGGCAACGTCGAt > 2:584201/1‑143 (MQ=25) cTTACCCGGCCGACAAAGGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGAt < 2:571017/143‑1 (MQ=255) cGGCCTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCGGACGTGGTTGCTATTCAGATAACGTCGATTTCAGc < 2:323453/143‑1 (MQ=255) cTACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACt > 2:200895/1‑143 (MQ=255) tACAAAAGCATG‑AAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGAGAACGTCGATTTCAGCGACTg > 2:231125/1‑143 (MQ=255) aaaaGCATG‑AAATTCAATATATTGCCGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGGTTGGCGCTTGCCACCAGGACTGGCTAGGTCTGCAGGCGTGGTTGCTCTTCAGCTACCGTCGCTTTCAGCGACTGCCg > 1:166114/1‑143 (MQ=12) | AGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACG > NC_000913/699327‑699602 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |