New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 465407 =43 (1.070)3 (0.080) 3/274 5.9 6.6% coding (141/696 nt) queC 7‑cyano‑7‑deazaguanine (preQ0) synthase; queuosine biosynthesis
?NC_000913 465460 = 44 (1.140)coding (88/696 nt) queC 7‑cyano‑7‑deazaguanine (preQ0) synthase; queuosine biosynthesis
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCTATGAAACGTGCTGTCGTTGTGTTCAGTGGAGGTCAGGATTCCACCACCTGTCTGGTGCAGGCATTACAACAATATGATGAAGTCCATTGCGTGACGTTCGATTACGGTCAGCGGCATCGCGCAGAAATCGACGTGGCACGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/465550‑465407
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cacgcAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTGCGATTAACC  >  NC_000913/465460‑465599
                                                                                                                                                                                                                                                                                            
TCTATGAAACGTGCTGTCGTTGTGTTCAGTGGAGGTCAGGATTCCACCACCTGTCTGGTGCAGGCATTACAACAATATGATGAAGTCCATTGCGTGACGTTCGATTACGGTCAGCGGCATCGCGCAGAAATCGACGTGGCACG                                                                                                                                               <  2:461403/143‑1
  TATGAAACGTGCTGTCGTTGTGTTCAGTGGAGGTCAGGATTCCACCACCTGTCCGGTGCAGGCATTACAACAATATGATGACGTCCATTGCGTGACGTTCGATTACGGTCAGCGGCATCGCGCAGAAAACGACGTGGCACGtg                                                                                                                                             >  2:278211/1‑141
                                                                                    GTCCATTGCGTGACGTTCGATTACGGTCAGCGGCATCGCGCAGAAATCGACGTGGCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGT                                                           >  1:63654/1‑143
                                                                                       CATTGCGTGACGTTCGATTACGGTCAGCGGCATCGCGCAGAAATCGACGTGGCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTC                                                        >  1:325983/1‑143
                                                                                                                            CAGAAATCGACGTGGCACGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGT                   <  2:63654/143‑1
                                                                                                                                             CGCAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTGCGATTAACC  <  1:441346/143‑1
                                                                                                                                                                                                                                                                                            
TCTATGAAACGTGCTGTCGTTGTGTTCAGTGGAGGTCAGGATTCCACCACCTGTCTGGTGCAGGCATTACAACAATATGATGAAGTCCATTGCGTGACGTTCGATTACGGTCAGCGGCATCGCGCAGAAATCGACGTGGCACGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/465550‑465407
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cacgcAATGGACTTCATCATATTGTTGTAATGCCTGCACCAGACAGGTGGTGGAATCCTGACCTCCACTGAACACAACGACAGCACGTTTCATAGATAATCCTGCTTGAACAATAAAAGCGTTATGGTAACGCCTGCGATTAACC  >  NC_000913/465460‑465599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.