Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,951,735 Δ326 bp pseudogene (194‑519/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3951735 3952060 326 46 [0] [0] 45 ilvG pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 39517340 (0.000)41 (1.080) 36/268 0.1 100% pseudogene (193/663 nt) ilvG pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
?NC_000913 3952061 = 0 (0.000)pseudogene (520/663 nt) ilvG pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

CGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3951592‑3951734
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCA  >  NC_000913/3952061‑3952201
                                                                                                                                                                                                                                                                                             
cgtcccTCCTGGTGACGCTATCTACGCGCCGTTGTTGTTAAGACAACTGTCTGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGCGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAA                                                                                                                                               <  2:102544/137‑1
   CCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGAC                                                                                                                                            >  1:629049/1‑143
     agctCGGTTACGTTATCTTCGCGCCGTTGTTGTTAAAACAATTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCTCACACTCGCCCGGAAAGACCA                                                                                                                                          <  2:44480/139‑1
             gacgtcATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAG                                                                                                                                  <  2:546499/137‑1
                GCTATCTACGCGCCGTTGTTGTTAAAACAAATGTCGATCAGGTAATCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATAGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGG                                                                                                                               >  2:156060/1‑143
                    TCTACGAGCCCTCGTGGTTCGGACTACGGTCGGAGCGTAAGCCTGCGGAGTGCGGCGTGACCACAGCTGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACT                                                                                                                           <  2:587170/143‑1
                       ACGCGCCGTTGTTGTTAAAACAACTGTCGGATCTTAACGCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGA                                                                                                                        <  1:686403/143‑1
                        CGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGAC                                                                                                                       <  2:192084/143‑1
                          CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACAC                                                                                                                     >  1:197299/1‑143
                             CGTTGTTGTTAAAACAACTGTCGGATCGTAAACCCGCGGATTGCGTCGTGACCACAGATGTGGGGCACCACCAGATGCGGGCTGCGCAGCCCATCGCCCACACTCGCCCGGAACGACCAGGCTGAAGCGGCACTCGACACCcc                                                                                                                  >  2:698421/1‑141
                                   TGTTAAAACAACTGTCGGATCGTAAACCTGCGGTTTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGGCCAGGTTGAAGCGGGACACGACACCATGGTGAA                                                                                                            >  2:300116/1‑143
                                     TTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACACGACACCATGCTGAACA                                                                                                          >  2:543952/1‑143
                                         AACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGg                                                                                                      >  1:184265/1‑142
                                             ACTGTCGGAACGAAAACCGGCAGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGAGAAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGG                                                                                                  <  1:356760/143‑1
                                                    GATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACGAGATGTGGGCTGCGCAGCACCTCCCCCACCTTTCCCTGGATAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAGCAGTGATGGGCCCTACC                                                                                           >  1:454009/1‑143
                                                     ATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCAGACCT                                                                                          >  2:219925/1‑143
                                                              CCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACCCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATG                                                                                  <  2:325573/143‑1
                                                                                tccgCAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGTTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTG                                                                <  2:151313/139‑1
                                                                                  CACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCCGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGcg                                                              >  2:217877/1‑141
                                                                                  CACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCCTGCTGCACAGTGATCGGCCATACCTGCTTCATGTCTCAATAGACCAACTTGAG                                                              >  1:3775/1‑143
                                                                                         GTGGGGCAGTACCAGATGTGGGCTGCGCAGCACATCGCCCACCCTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCT                                                       <  2:364122/143‑1
                                                                                            GGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGGTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCCTACCTGCGTCATGTCTCAATCGGCGAACCTGGGGACGGCGGGC                                                    >  1:581188/1‑143
                                                                                            GGGCAGCACCAGATGTGGGCTGCGCAGCACAGCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGC                                                    <  2:30687/143‑1
                                                                                                AGCACCAGATGTGGGCTGCGCAGCACCTCGAACACATGCGCTCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCT                                                <  1:492420/143‑1
                                                                                                AGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGCAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCCGCCCGCc                                                >  2:82013/1‑142
                                                                                                   ACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGTCACCATGCTGAACAGTGATGGGCCATACCTGCTTCCTGTCTCAATCGACGGACTTGAGAACGTCTGGCCGCTGGT                                             >  2:362662/1‑143
                                                                                                   ACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGACCTTGAGGACGTCTGGCCGCTGGT                                             >  1:639636/1‑143
                                                                                                   ACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGT                                             <  2:197299/143‑1
                                                                                                       cATGTGGGCTGCGCAGCACCTCGCCCACACCCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCG                                         <  1:298363/142‑1
                                                                                                       GATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCG                                         >  1:642365/1‑143
                                                                                                          GTGGGCTGCCCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCT                                      <  1:502694/143‑1
                                                                                                             GGCTGCTCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATTCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGATAACGTCTGGCCGCTGGTGCCGCCTGGC                                   <  2:381376/143‑1
                                                                                                             GGCTGCGCAGCACAGCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAATTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGC                                   <  2:380699/143‑1
                                                                                                                TGCGCAGCACGTCGCCCACCCTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGAGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCC                                <  2:261800/143‑1
                                                                                                                  CGCAGCACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTTGCGCCcg                              >  1:281324/1‑141
                                                                                                                       CACATCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTCCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGCGCCACCTCGCGCCCGGAATT                         >  2:183042/1‑143
                                                                                                                          ATCGCCCCCACCCGCCCGGCAACACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATCCCTTCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAG                      <  1:246861/143‑1
                                                                                                                           TCGCCCACACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGA                     >  1:444154/1‑143
                                                                                                                                  CACTCGCCCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTG              <  1:579508/143‑1
                                                                                                                                    cgCGCCCGGAAACACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCGTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGA            <  1:144396/141‑1
                                                                                                                                    CTCGCCCGGAAAGACCACGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGACCGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGA            <  1:10925/143‑1
                                                                                                                                    CTCGACCGGCAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGA            <  1:236413/143‑1
                                                                                                                                         CCGGAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAAT       >  1:423933/1‑143
                                                                                                                                            GAAAGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGCGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTAT    <  2:20609/143‑1
                                                                                                                                             AAAGACCACGTTGAAGCGGCACTCGACACCCTGCTGAACAGTGATCGGCCATACCTGCTTCATGTCTCAACCGACGAACTTGAGCACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATC   <  1:156060/143‑1
                                                                                                                                              AAGACCAGGTTGAAGCGGCACTCGACACAATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAGTGTTGGAGAGATTATCA  >  1:537229/1‑143
                                                                                                                                                                                                                                                                                             
CGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCGCCCACACTCGCCCGGAAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3951592‑3951734
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cccggaaaGACCAGGTTGAAGCGGCACTCGACACCATGCTGAACAGTGATGGGCCATACCTGCTTCATGTCTCAATCGACGAACTTGAGAACGTCTGGCCGCTGGTGCCGCCTGGCGCCAGTAATTCAGAAATGTTGGAGAAATTATCA  >  NC_000913/3952061‑3952201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.