Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,697,375 T→C Q222Q (CAA→CAG hdhA ← 7‑alpha‑hydroxysteroid dehydrogenase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,697,3750TC100.0% 84.2 / NA 29Q222Q (CAA→CAGhdhA7‑alpha‑hydroxysteroid dehydrogenase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/12);  total (17/12)

CAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAGG  >  NC_000913/1697238‑1697513
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caacaCGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGAAAACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCgg                                                                                                                                       >  1:590243/1‑143 (MQ=255)
             tgttTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGt                                                                                                                          >  1:461499/1‑143 (MQ=255)
              gttTACCGGTCCGTTAGTGTATTACTTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCCCCCACCCCGCCCGCACCCGGCGACCAACGACACATCGCTTCCTAAGCACTATCCTGCCGTTGGcccccacccc                                                                                                                         >  2:478116/1‑136 (MQ=255)
                  aCCGGCCCGTTAGTGCATTAATTGAGCTCCTGGACCCCACCACCGTAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGAt                                                                                                                     <  1:160158/143‑1 (MQ=255)
                      gtcctgtAGCGTATTAGTCTAGATCCTGTACCCCACCACCGGAGACGGTGAGAATCTCTCTGCGTCCGCAGCTCTCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGc                                                                                                                 <  2:436505/137‑1 (MQ=255)
                           ttAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGCCGGTGAGCATTTGTCCACTTAAACAGCTCGCAACAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGagtg                                                                                                            >  1:30604/1‑143 (MQ=255)
                           ttAGCGTATTAATTGAGCTCCTGTACCCCACCCCCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGtgtg                                                                                                            <  1:317738/143‑1 (MQ=255)
                                 tATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGGGAGAATGCGGCCGCTTACCCAGCTCGCAGCAGGCGCGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCCGACGTCTGATAGGCGTGTCCTggca                                                                                                      >  2:495979/1‑140 (MQ=255)
                                    tAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACAt                                                                                                   >  1:123900/1‑143 (MQ=255)
                                            cTCCTGTACCCCCCCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGtt                                                                                           <  2:276887/143‑1 (MQ=255)
                                                     cccACCACCGGAGACTGTGAGAATTTGGCCCCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTg                                                                                  <  1:437322/143‑1 (MQ=255)
                                                     cccACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTg                                                                                  <  1:550655/143‑1 (MQ=255)
                                                     cccACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTg                                                                                  <  1:277141/143‑1 (MQ=255)
                                                     cccACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATCTTTTGTTCAAGTTCTg                                                                                  >  2:38788/1‑143 (MQ=255)
                                                                       gagaATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCCGACGTCTGATCGACGTGTGCTGTAACATTTTTTCTTCAATTGCTCATGTAGTAACGGATTTcg                                                                >  2:620187/1‑142 (MQ=255)
                                                                         gaATTGGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAgg                                                              <  2:682507/143‑1 (MQ=255)
                                                                              tGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATc                                                         <  2:590243/143‑1 (MQ=255)
                                                                              tGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATc                                                         <  2:393108/143‑1 (MQ=255)
                                                                               gTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTATGAGCGGCGTGTGCTGTGGCGTCTCGTGTTCAATTTCTGGTGTAATAACTGATTTCAGGGCATct                                                        >  1:99806/1‑142 (MQ=255)
                                                                                     ttACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTATTCACGTCTTTCAGGGCATCGGTTCat                                                  >  2:4324/1‑143 (MQ=255)
                                                                                      tACCCAGCTCGCAGCAGGCGAGCAAAGGACCAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAata                                                 <  2:219049/143‑1 (MQ=255)
                                                                                         ccAGCTCGCAGCAGGCGAGCAAAGGCACAGCGCTGCGTAAGCAATAACCTGCGGTTGGCCCAGACGTCTGATCGGCGTTTCCCCCAACATGTTCTGTTCAATCTCTCGTGTACTAACGAATCTCAGCGCATCGGTTAATATTg                                              >  2:123200/1‑143 (MQ=255)
                                                                                         ccAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTCATATTg                                              >  1:566430/1‑143 (MQ=255)
                                                                                         ccAGCTCGCAGCAGGCAAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCAGTTAATATTg                                              >  1:87028/1‑143 (MQ=255)
                                                                                                  agcagGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGgcg                                     >  1:441773/1‑143 (MQ=255)
                                                                                                                       cgcTGCGTTAGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGActat                >  1:382701/1‑143 (MQ=255)
                                                                                                                        gcTGCGTTAGCAATATCCTGCGGTTAGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATAGGTTAATATTGGCCCCGGCGCAATGCCATTGACCCGaagact               >  2:68935/1‑139 (MQ=255)
                                                                                                                                aGCAATATCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAACAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCAc       >  1:381059/1‑143 (MQ=255)
                                                                                                                                     tatCCTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGTTGTTACATTTTTTCTTCAATTTCTGGTGTAATAACGCATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAgg  <  2:214773/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CAACACGCACGGTTGTTTACCGGTCCGTTAGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGACGTCTGATCGGCGTGTGCTGTAACATTTTTTGTTCAATTTCTGGTGTAATAACGGATTTCAGGGCATCGGTTAATATTGCCCCCGGCGCAATGCCATTTACCCGAATATTTTTTTCACCCAGG  >  NC_000913/1697238‑1697513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: