New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 222402164 (0.990)3 (0.050) 3/282 10.1 4.9% coding (850/933 nt) pbpG D‑alanyl‑D‑alanine endopeptidase
?NC_000913 2742804 = 54 (0.840)coding (422/1227 nt) yfiN putative membrane‑anchored diguanylate cyclase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCG  >  NC_000913/2742662‑2742946
                                                                                                                                              |                                                                                                                                              
ggccGTGATAAGGAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTGTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTgg                                                                                                                                                <  2:861931/140‑1 (MQ=255)
gTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTgg                                                                                                                                                >  2:526840/1‑143 (MQ=255)
gTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTgg                                                                                                                                                >  1:186911/1‑143 (MQ=255)
   cGTGATAAGCAGCGAGATATTCTGGCATCCTGGCATTACACCCGTACGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCCCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGaa                                                                                                                                            <  2:944364/143‑1 (MQ=255)
   cGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGa                                                                                                                                             >  1:1077276/1‑143 (MQ=255)
        ggagcaggAGAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGTCACTGGCTCTTCCCTGCCCCCATCTTTCCATTAACTTTCTACAATAGTGAATCCATTGTCGAAGTAc                                                                                                                                        <  2:834850/141‑1 (MQ=255)
         aaGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGCAACCATTGGCGAAGTACg                                                                                                                                       >  2:140509/1‑143 (MQ=255)
          agcagcAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGc                                                                                                                                      <  1:343140/143‑1 (MQ=255)
          agcagcAAAAGATTCTGGCATCCTAGCATTACACCCGTAAGGATCCCGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGc                                                                                                                                      <  1:825752/143‑1 (MQ=255)
              gcaAAATATTCTGGCATCCTGGCATTACACCCGTACGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTCCCCCCATCATTCACCCCATTCGTCCCAATGGTGCAACCATTCGCGCAGCACGCTTaa                                                                                                                                  >  2:275029/1‑143 (MQ=255)
              gcaAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTaa                                                                                                                                  <  1:682154/143‑1 (MQ=255)
              gcaAAATATTCTGGCAGTCTGGCATTACACCCGTAAGGATCCAGGCGCTACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTaa                                                                                                                                  <  2:671160/143‑1 (MQ=255)
               caAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGCAGTACGCTTAAc                                                                                                                                 >  1:190787/1‑143 (MQ=255)
                aaaaTACTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAAcc                                                                                                                                <  1:100427/143‑1 (MQ=255)
                    tatTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCta                                                                                                                            >  1:4567/1‑142 (MQ=255)
                      ttCTGGCATCCTGGCATTACACCCGTAACCCTCCAGGCGATACTTTCAGTAATTTCATAAGCAACAAACAAAACACTACCCCAATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCAcgc                                                                                                                          >  1:7591/1‑143 (MQ=255)
                        cTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCACCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGa                                                                                                                        <  1:165441/143‑1 (MQ=255)
                             aTCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGGCAGtt                                                                                                                   >  2:592034/1‑143 (MQ=255)
                             aTCCCGGCATTACACCCGTAAGGATCCAGGCGATACCTTCAGTAATTTCATACGCCACTGGCTTTTCCCTGCCCCCATCATTCACCCGATTCGTCAAAATGGTGAAACCATTAGCGAATTACACTTAAAAGCTAGCGACAgca                                                                                                                   >  1:526145/1‑141 (MQ=255)
                                 tGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGATCGCGACAATTCAAt                                                                                                               >  2:620378/1‑143 (MQ=255)
                                          acCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGAAAGGGTCAGTTCAATCAGCCAttt                                                                                                      >  2:282109/1‑143 (MQ=255)
                                                  ggATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGt                                                                                              <  1:344967/143‑1 (MQ=255)
                                                     tCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATCTTACCTGGtttt                                                                                           >  2:395504/1‑143 (MQ=255)
                                                       cAGGCGATGCTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtcg                                                                                         <  2:670920/143‑1 (MQ=255)
                                                       cAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtcg                                                                                         <  1:526840/143‑1 (MQ=255)
                                                        aGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtcgc                                                                                        <  2:227040/143‑1 (MQ=255)
                                                             aTACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATNCAGCCGATTCGTCACAATGGTGAAACCCTTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGcc                                                                                   <  1:698357/143‑1 (MQ=255)
                                                                 tttCCGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTAc                                                                               <  1:886391/143‑1 (MQ=255)
                                                                 ttccAGTAATTTCCTAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTAc                                                                               <  2:658005/140‑1 (MQ=255)
                                                                      gTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATATTATCAAGTTTTAGCTCGCAGTACTGaac                                                                          >  1:353561/1‑141 (MQ=255)
                                                                            tCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGt                                                                    >  2:1078718/1‑143 (MQ=255)
                                                                               tAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTAtt                                                                 >  1:890844/1‑143 (MQ=255)
                                                                               tAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGCTCAATCAGCCATTTTATCTGGTTTTCGCCCGCCGTACTCACCGGTTGTAtt                                                                 >  2:1052087/1‑143 (MQ=255)
                                                                                 aGCCACGGGCGCTGCCCGGCCCCCATCATTCAGGCGATTCGTCACTATTGTGAAACCATTGGCGAAGTACGCTGAACCGCGCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTct                                                               <  2:502689/143‑1 (MQ=255)
                                                                                     aCTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGCAGTACGCTTAACCGCTCGCGACCGTTCAATCAGCCATTTTATCTGGGTTTCGCTCGCCGTACTGCCCGGTTGTATTcggctg                                                           >  1:638733/1‑143 (MQ=255)
                                                                                                 cTGCCCCCATCATTCAGCCGATTCGTTACAATGGTGAAATCATTGGCTAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATc                                               <  2:7591/143‑1 (MQ=255)
                                                                                                 cTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATc                                               >  2:219842/1‑143 (MQ=255)
                                                                                                 cTGCCCCCATCATGCAGTCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATc                                               <  2:679441/143‑1 (MQ=255)
                                                                                                  tcccccccTCATTCACCCGACTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCATCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCg                                              <  1:287944/141‑1 (MQ=255)
                                                                                                       ccATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTTGCGACAGTTCCATCTGCCCTTTTATCTGGTTTTCGCTCCCTGTACTGACCGGTTGTCTTCTGCTGGCATCAGCCCTCGccctc                                         >  1:772214/1‑139 (MQ=255)
                                                                                                          tcatTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTAcc                                      <  2:746624/143‑1 (MQ=255)
                                                                                                           ccttCGGCGGATTCGTCACAGTGGTGAACCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTGTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTAccc                                     <  2:363280/141‑1 (MQ=255)
                                                                                                            atTCAGCGGATTCGTCACAATTGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTCTCTCGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCt                                    <  1:906594/143‑1 (MQ=255)
                                                                                                              tCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCa                                  <  1:282109/143‑1 (MQ=255)
                                                                                                                 gCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCAccc                               >  1:893205/1‑143 (MQ=255)
                                                                                                                      gtCGTCAGTATGCGGAAACCATTGGCGAAGGTCTCTGAACCGCGCGCGACAGTTCAATCGGCCATGTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATGCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCAt                          <  2:30140/142‑1 (MQ=255)
                                                                                                                                   tGACACCATTGGCGACGTACGCCTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGcc             <  1:240273/143‑1 (MQ=255)
                                                                                                                                     aaaCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTg           <  1:1078718/143‑1 (MQ=255)
                                                                                                                                     aaaCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTg           >  1:1074003/1‑143 (MQ=255)
                                                                                                                                     aaaCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTg           <  1:1113021/143‑1 (MQ=255)
                                                                                                                                       aCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGt         <  1:711333/143‑1 (MQ=255)
                                                                                                                                        ccATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTa        >  1:611569/1‑143 (MQ=255)
                                                                                                                                         cATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGAACCTGCAAGCGCTTTTACCCTCACCCGCCGTTTGCACAATGGCCTGGTAg       >  2:1003240/1‑143 (MQ=255)
                                                                                                                                          atgggCGAAGTACGCTTAGCCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGa      <  2:1044095/140‑1 (MQ=255)
                                                                                                                                           ttGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGac     >  1:1064333/1‑142 (MQ=255)
                                                                                                                                             ggCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTGGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAGTGGGCTGGTAGAAgc   >  1:353942/1‑143 (MQ=255)
                                                                                                                                             ggCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTGTTCGCTCGCCGTACACACCGGGTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGGAgc   >  1:165841/1‑143 (MQ=255)
                                                                                                                                              gCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAgcg  >  1:641964/1‑143 (MQ=255)
                                                                                                                                              |                                                                                                                                              
GTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCG  >  NC_000913/2742662‑2742946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.