Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	88 [0]	[0] 88	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	85 (1.350)	70/276	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

AAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCT  >  NC_000913/1299356‑1299639                                                                                                                                               |                                                                                                                                              gaatTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATAATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAA                                                                                                                                               <  2:479339‑M1/142‑1 (MQ=255)  aaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTGTTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                                                                                                              <  1:266440‑M1/143‑2 (MQ=255)     tACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                           <  1:810610‑M1/143‑5 (MQ=255)     tACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCCCGCCAGGTGGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataa                                                                                                                                           <  1:97053‑M1/143‑5 (MQ=255)       cAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataata                                                                                                                                         <  1:777436‑M1/143‑7 (MQ=255)        agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                                                                                                                        <  2:66478‑M1/143‑8 (MQ=255)         tgtagttataAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataat                                                                                                                                       <  1:1090096‑M1/141‑9 (MQ=255)          ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatc                                                                                                                                      >  1:188902‑M1/1‑134 (MQ=255)              ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                  >  1:67791‑M1/1‑130 (MQ=255)              ttaTAAGGACTTCCTTAACTGCTCCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTCGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                  <  2:177219‑M1/143‑14 (MQ=255)               tataAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattg                                                                                                                                 <  1:994217‑M1/143‑15 (MQ=255)                ataAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgt                                                                                                                                >  2:963920‑M1/1‑128 (MQ=255)                 taAGGCTTTCCTTAACTGCTCCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                               <  2:1153104‑M1/143‑17 (MQ=255)                 taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                               <  2:1106541‑M1/143‑17 (MQ=255)                  aaGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAACGTGCTAAataataatcaattgtta                                                                                                                              >  1:920104‑M1/1‑126 (MQ=255)                         tCCTTAACTGCGTCTCACAACCATCATGTTATTTTACCTACATGATGATCCTGTGCGGGCTGCACAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                       <  2:288443‑M1/143‑25 (MQ=255)                            ttAACTGCTTCTCCTCGCCATCATGTTATGTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGAGATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgta                                                                                                                    <  2:177059‑M1/143‑28 (MQ=255)                            ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATCTTCTTCATCATGCTGCTTTTTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                    >  1:1154553‑M1/1‑116 (MQ=255)                            ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                    >  1:784661‑M1/1‑116 (MQ=255)                            ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                    >  2:254082‑M1/1‑116 (MQ=255)                                cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatt                                                                                                                >  1:642525‑M1/1‑112 (MQ=255)                                 tGCTTCTCCTCACCTTCATGTTATTTTCGCCACATCATAATCCTGTGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTAGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                               <  2:174634‑M1/143‑33 (MQ=255)                                 tGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattt                                                                                                               <  2:1034086‑M1/143‑33 (MQ=255)                                   cTTCTCCTCACCTTCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTAGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                             <  2:1155187‑M1/143‑35 (MQ=255)                                    ttCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcac                                                                                                            >  1:1052822‑M1/1‑108 (MQ=255)                                         ctcACCATCATGTTATTTTCGCCACATCATATGCCGGGTCGTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                       <  2:1126109‑M1/143‑41 (MQ=255)                                         ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                       >  2:919101‑M1/1‑103 (MQ=255)                                          tcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgg                                                                                                      >  1:893814‑M1/1‑102 (MQ=255)                                            aCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGACATGATATAATTAATCCCCCTGCCTTTGGTAGAGGAAAGGGCTAAataataatcaattgttcaatcattgtgaatttcactactggaa                                                                                                    >  2:497762‑M1/1‑100 (MQ=255)                                              catcatGTTATTTTTTTTTCTTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgtaact                                                                                                  >  2:597329‑M1/1‑98 (MQ=255)                                                  gtgTTTTTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaa                                                                                              <  1:923597‑M1/142‑50 (MQ=255)                                                    gctagtttcgcccCCTCCTCTTCCTACGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                            <  1:207341‑M1/138‑52 (MQ=255)                                                    gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcgattgttaaattattgtgcatttcactactggaactgtaatc                                                                                            >  1:780176‑M1/1‑92 (MQ=255)                                                    gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                            <  1:418650‑M1/143‑52 (MQ=255)                                                     ttATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                           <  1:457367‑M1/143‑53 (MQ=255)                                                     tgattTTCGCTACATCATACTCCTGTGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatca                                                                                           <  2:219186‑M1/141‑53 (MQ=255)                                                         tttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaa                                                                                       >  1:176877‑M1/1‑87 (MQ=255)                                                         tctcgCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGCAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaa                                                                                       <  1:863422‑M1/141‑57 (MQ=255)                                                         gttcGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaa                                                                                       <  2:262059‑M1/142‑57 (MQ=255)                                                           tCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaag                                                                                     <  2:707917‑M1/143‑59 (MQ=255)                                                             gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                                                                                                                   <  1:150159‑M1/95‑13 (MQ=255)                                                             gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaat                                                                                                                                   >  2:150159‑M1/1‑83 (MQ=255)                                                                acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                <  2:418989‑M1/143‑64 (MQ=255)                                                                acaTCATAATCCTGGGCTGGCTGGAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                                >  2:896104‑M1/1‑80 (MQ=255)                                                                  atcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTACataataatcaattgttaaattattgtgcatttcactactggaactctaatcagaaaagatagaca                                                                              >  2:516672‑M1/1‑78 (MQ=255)                                                                  atcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagaca                                                                              <  1:455633‑M1/143‑66 (MQ=255)                                                                      tAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgct                                                                          >  2:925377‑M1/1‑74 (MQ=255)                                                                       aaTCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTTGAGGAAAGTGCTAAataataatcatttgttaaattattgtgcatttcactactggaactgtaatcaggaaagatagagatgctt                                                                         >  2:578865‑M1/1‑73 (MQ=255)                                                                        agcctgCGCTTGCCGAAGAATAACTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgctta                                                                        <  1:421846‑M1/141‑72 (MQ=255)                                                                                cTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGGTAAataataatcaattgttaaattattgtgcatttaacgactggaacggtaatcagaagagatagacatgattagccaatct                                                                >  2:159016‑M1/1‑64 (MQ=255)                                                                                   gCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctcta                                                             >  1:101888‑M1/1‑61 (MQ=255)                                                                                     tGAAGAATAATTGAAATGATATTATTAATTCCACTGCGTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactaatggaactgtaatcagaaaagataggcatgcttagtcaatctctatt                                                           >  1:359575‑M1/1‑59 (MQ=255)                                                                                     tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttagtgtatggtggattgcttaacaggaactgtaatcagaaaagatagacatgcttagccaatctctatt                                                           >  2:825864‑M1/1‑59 (MQ=255)                                                                                      gaacaaTAATTGAAATGACACTATTCACTCCACTGCCTTCACTGCACGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattt                                                          <  1:486794‑M1/143‑86 (MQ=255)                                                                                        agaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttga                                                        <  2:904264‑M1/143‑88 (MQ=255)                                                                                          aataatTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                      <  1:420561‑M1/143‑90 (MQ=255)                                                                                               ctgAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataatactcaattgttaaattcttgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaaactctctttgattcacat                                                 >  2:1111547‑M1/2‑49 (MQ=255)                                                                                                tGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattg                                                >  1:119159‑M1/1‑48 (MQ=255)                                                                                                  aaaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaa                                              >  2:897836‑M1/1‑46 (MQ=255)                                                                                                   aaTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                             <  1:813301‑M1/143‑99 (MQ=255)                                                                                                     tGATATTATTAATTCCACTGCTTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcataaaagatagacatgcttagccaatctctatttgattgaattgaaaga                                           <  2:290660‑M1/143‑101 (MQ=255)                                                                                                     tGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaaga                                           >  2:227793‑M1/1‑43 (MQ=255)                                                                                                         attattAATTCCACTGCTTTTGGTAGAGGAAAGTGTTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagtcatgcttagccaatctctatttgattgaattgaaagatgtt                                       <  2:999454‑M1/143‑105 (MQ=255)                                                                                                             ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatccctctttgattgaattgaaagatgtttgtt                                   >  2:360315‑M1/1‑35 (MQ=255)                                                                                                               aaTCCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcacgatcggaactgtaatcagaaaagatagacatgcctagccaatctctatttgattgaattgaaagatgtttgttaa                                 <  1:109857‑M1/143‑111 (MQ=255)                                                                                                                    cACTGCTTGTGGTTGAGGACAGTGCTAAagaataatcaattgttaaattattgtgcatttcactactggaactgtaatcataaaagatagacatgcttagccaatctctatttgattgaattgaaagttgtttgttaaggcat                            <  2:360502‑M1/143‑116 (MQ=255)                                                                                                                    cACTGCCTTTGGTAGATGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcat                            <  2:1037831‑M1/143‑116 (MQ=255)                                                                                                                    cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgcaagatgtttgttaaggcat                            >  1:228539‑M1/1‑28 (MQ=255)                                                                                                                    cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcat                            <  2:786362‑M1/143‑116 (MQ=255)                                                                                                                     aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaatgcatg                           <  2:148092‑M1/143‑117 (MQ=255)                                                                                                                     aCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattcaattgaaagatgtttgttaaggcatg                           >  1:110775‑M1/1‑27 (MQ=255)                                                                                                                        gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                        <  2:26531‑M1/143‑120 (MQ=255)                                                                                                                          cTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcagggatgc                      >  1:1116431‑M1/1‑22 (MQ=255)                                                                                                                            ttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaa                    <  1:984627‑M1/143‑124 (MQ=255)                                                                                                                            tgagtagAGGAAAGAGCTAAagaataatcaattgttaaattaatgtgcatttcactactggaactgtaatcataaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttcgttaagccaagcatgcaa                    <  2:837500‑M1/140‑124 (MQ=255)                                                                                                                             tGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcgtagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaag                   <  2:188902‑M1/143‑125 (MQ=255)                                                                                                                             tGGTAGAGGAAAGCGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaag                   <  1:702925‑M1/143‑125 (MQ=255)                                                                                                                              ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                      >  1:187430‑M1/1‑18 (MQ=255)                                                                                                                              ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                      <  2:187430‑M1/91‑74 (MQ=255)                                                                                                                                tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagcta                >  2:10810‑M1/1‑16 (MQ=255)                                                                                                                                 agagGAAAGTGCTAAattataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaagctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctat               <  2:174325‑M1/143‑129 (MQ=255)                                                                                                                                  gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata              <  1:919101‑M1/143‑130 (MQ=255)                                                                                                                                     gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagat           >  2:656976‑M1/1‑11 (MQ=255)                                                                                                                                      aaaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagatt          >  1:195203‑M1/1‑10 (MQ=255)                                                                                                                                       aaGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattc         <  1:453157‑M1/143‑135 (MQ=255)                                                                                                                                          tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctga      >  2:386792‑M1/1‑6 (MQ=255)                                                                                                                                            cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgata    >  2:123715‑M1/1‑4 (MQ=255)                                                                                                                                              aaatcataatcaagtgttaaattattgtgcatttcactactgtaagtgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacg  <  2:346278‑M1/143‑142 (MQ=255)                                                                                                                                               |                                                                                                                                              AAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCT  >  NC_000913/1299356‑1299639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑