Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,700,725	A→T	I55F (ATC→TTC)	yhjV →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,700,725	0	A	T	97.0%	196.8 / NA	66	I55F (ATC→TTC)	yhjV	putative transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (25/39);  minor base G (0/2);  total (25/41)
Fisher's exact test for biased strand distribution p-value = 5.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CAATCAGAAATTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGATCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAATTTTCCTCGGGGTTATC  >  NC_000913/3700589‑3700862                                                                                                                                         |                                                                                                                                          caATCAGAAATTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTg                                                                                                                                     <  1:19741/143‑1 (MQ=255)  gaTCAGAAATTGGCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGc                                                                                                                                    <  2:990801/142‑1 (MQ=255)  aaTCAGAAATTGTCGTTTACACGCTACGATTTCTGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGc                                                                                                                                    <  2:374883/143‑1 (MQ=255)    tCAGAAATTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTAATTATTACCGCAGCGTTCATTGAtt                                                                                                                                  >  2:743447/1‑143 (MQ=255)    gcAGAAAGTGCCGTTTACACGCGACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCtt                                                                                                                                  <  1:198125/142‑1 (MQ=255)      aGAAATTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTAt                                                                                                                                <  1:695406/143‑1 (MQ=255)        aaaTTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATcc                                                                                                                              >  1:242541/1‑143 (MQ=255)          aTTGCCGTTTCCACGCTACGACTTCGGCTGGGATTTATTATGCATAGGAATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACGAATTGGCTTGTAGGGGCTTTGGGTAGTTATTGCCGCAGCGTTCGTGGCTTATCGTg                                                                                                                            >  1:572864/1‑143 (MQ=255)                gTTTCCACGCTATGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCt                                                                                                                      <  1:226422/143‑1 (MQ=255)                 tttACACGCTACGACTTCGGATGGGTTTTATGATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTg                                                                                                                     <  1:725907/143‑1 (MQ=255)                  ttACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTgg                                                                                                                    <  1:658890/143‑1 (MQ=255)                  ttACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTgg                                                                                                                    <  1:696797/143‑1 (MQ=255)                      acGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATAGCGAAAGGAACCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAAGTTGGGTATTTATTACAGCAGCGTTCATAGCACCTCCTGCCACATGGGTAg                                                                                                                >  2:638369/1‑143 (MQ=255)                           aCGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTCGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAg                                                                                                           >  1:609117/1‑143 (MQ=255)                             gccctCGGCTGGGTGTTATTACGCAGCGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTCAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGa                                                                                                         <  1:381276/139‑1 (MQ=255)                                ttCGGCTGGGTTTTTTTTTGCATTGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATAATGTCTTCTGGGTAGTGCAGGACat                                                                                                      <  2:144483/143‑1 (MQ=255)                                  cGGCTGGGTTTCATTATGCAGAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACattt                                                                                                    <  1:129958/143‑1 (MQ=255)                                    gCTGGGTTTGATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGACCGGCATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACatttat                                                                                                  <  2:292432/143‑1 (MQ=255)                                    gCTGGGTGTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACatttat                                                                                                  <  1:455004/143‑1 (MQ=255)                                        ggTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGCATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGCCATTTATTTaa                                                                                              >  1:436949/1‑143 (MQ=255)                                         gTTTTATTATGCGTAGGCATGGCGATCGGTGCCGGAACCGTGCTGAGGCGAGTACAAATTGGCTTGAAGGGAATTTGGGGATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTaaa                                                                                             <  1:478638/143‑1 (MQ=255)                                          ttttATTTCGCATAGGCTTCGCGATTTGTTTCGGAACCGTGCTGATTCCAGTACAAATTGGCTTGAAGTGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTaaaa                                                                                            <  1:161436/143‑1 (MQ=255)                                          ttttATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTaata                                                                                            >  2:419470/1‑141 (MQ=255)                                           tttATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTGGTGCAGGGCCTTTATTTaaaca                                                                                           >  2:1142922/1‑141 (MQ=255)                                            ttattaTGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCGGGACATTTATTTaacaac                                                                                          >  2:764213/1‑143 (MQ=255)                                                 atGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCttt                                                                                     <  1:1016176/143‑1 (MQ=255)                                                   ggaTAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGCACAAATTGGCTTGAAGGGAATGTGGGGAGTTATTACCGCGGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCt                                                                                   <  1:669030/141‑1 (MQ=255)                                                   gCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCCTTCt                                                                                   >  1:234971/1‑143 (MQ=255)                                                         gCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTAGCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTGTCTGAAAGc                                                                             >  2:987598/1‑143 (MQ=255)                                                         gCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTGATTGCCGCAGCGTCCATTGCTTATCCTGCCACCTGGGTGGTGCCGGACATTTAGTTAAAAACCCTTGCTGACAGc                                                                             >  1:511508/1‑143 (MQ=255)                                                             ggCGTTTTGTTCCTGAACTGTTCTTATGTCATTACAAATTGGCTTGAATGGAATTTGTGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGTACATTTATTTAAAAACCCTTTCTGAAAGCGAtt                                                                         <  2:731012/143‑1 (MQ=255)                                                                gATTGGTGCCGGGTGCGTGGAGTTGCGAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCt                                                                      <  1:1090726/143‑1 (MQ=255)                                                                gATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCt                                                                      <  1:382737/143‑1 (MQ=255)                                                                gATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCGTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCt                                                                      >  2:640169/1‑143 (MQ=255)                                                                    gggacaggAGCCGTGCTTATGGCAGGACAAATTGGCTTGAAGGGCATTTGGGTATTTATTACCGCAGCGGTCATTTCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTaa                                                                  <  2:830141/137‑1 (MQ=255)                                                                    ggagCCGGAACCGTGCTGATGCCAGGAGAAAAAGGCGTGAAGGGAATTTGGGTATTTAGTACCGCAGCGTTCATTGCTTAGCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAGCCCTTTCTGAAAGCGATTCCTGTaa                                                                  <  1:502581/140‑1 (MQ=255)                                                                     gTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGGaaa                                                                 >  1:1029523/1‑142 (MQ=255)                                                                       gCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGa                                                               <  2:535764/143‑1 (MQ=255)                                                                        ccGGAACCTTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGAc                                                              <  1:1024337/143‑1 (MQ=255)                                                                        ccGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGAc                                                              >  1:1003559/1‑143 (MQ=255)                                                                        ccGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGAc                                                              >  2:450783/1‑143 (MQ=255)                                                                              ccGTGCTGATGCCAGTACAAATGGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCGCCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACAcc                                                        <  1:979414/143‑1 (MQ=255)                                                                                gTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGa                                                      <  1:1161393/143‑1 (MQ=255)                                                                                 tGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGat                                                     <  1:473605/143‑1 (MQ=255)                                                                                 tGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCGTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGat                                                     >  2:467470/1‑143 (MQ=255)                                                                                 tGCTGATGCCAGTACAAATTGGCGTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGat                                                     <  1:752339/143‑1 (MQ=255)                                                                                  gCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGata                                                    >  2:781185/1‑143 (MQ=255)                                                                                    tGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCAAtatt                                                  >  2:301626/1‑143 (MQ=255)                                                                                          cAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGt                                            >  2:1035181/1‑143 (MQ=255)                                                                                               gaaaTTGGCTTGAAGGTGAGTTGGGTATTTATTGCCGCAGCGTTCATTGCGTATCGGGCACCATGGGTAGTGCAGGACATTTATTTAAAATCCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTa                                       <  2:12495/142‑1 (MQ=255)                                                                                                gaaTTGGCGTGAAGGGAATGTGGGTAGTTATTACCGCAGCGTTCATTGCTTATCCTGCCGCCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTAc                                      <  2:349116/142‑1 (MQ=255)                                                                                                 aaTTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACAACATCGATATTATCAGTCATTAcc                                     <  2:143809/143‑1 (MQ=255)                                                                                                  aTTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGTGCCCGACCTATGTCATTGCGTTGTTTGTGCAGGACATTTATTTAAAAACCCTTTCTGAACGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCt                                    <  1:848324/143‑1 (MQ=255)                                                                                                    tGGCTTGAAGGGAATTTGGGTACTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTgg                                  <  1:492585/143‑1 (MQ=255)                                                                                                        ttGAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGGCATATATTTGAAAACCCTTTCTCAAAGCGATTGCTGTAATGACTAGCCCGCTACGATCAGTCATTAACTGCGgaa                              >  2:1135230/1‑143 (MQ=255)                                                                                                          gAAGGGAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGgaaga                            >  2:78664/1‑143 (MQ=255)                                                                                                              ggAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCGCCCTGTGTAGTGCAGGACCTTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTg                        <  1:1101509/143‑1 (MQ=255)                                                                                                              ggAATTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTg                        >  1:670543/1‑143 (MQ=255)                                                                                                                aaTTTGGGTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTggg                      <  2:1163746/143‑1 (MQ=255)                                                                                                                     tgttacttatgaCCGCAGCGGTTACTGCTTATCCTGCCTCCTGGGTAGTGCAGGACATTTATTTAAAATCCCTTTATGAAAGCGATTGCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAAtt                 <  2:1139809/137‑1 (MQ=255)                                                                                                                     gggTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAAtt                 <  1:888273/143‑1 (MQ=255)                                                                                                                       gTATTTATTACCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAAtttt               <  2:670543/143‑1 (MQ=255)                                                                                                                           ttattaCCGCAGCTTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACAGTTATTTACAAATCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAATTTTCCTc           <  2:349537/143‑1 (MQ=255)                                                                                                                           ttattaCCGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGCAGAACTGGGGAATTTTCCTc           >  1:1004337/1‑143 (MQ=255)                                                                                                                                  cGCAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAATTTTCCACGGGGTTa    >  1:170140/1‑143 (MQ=255)                                                                                                                                    cAGCGTTCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGAGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAATTTTCCTCGGGGTTATc  <  1:436665/143‑1 (MQ=255)                                                                                                                                         |                                                                                                                                          CAATCAGAAATTGCCGTTTACACGCTACGACTTCGGCTGGGTTTTATTATGCATAGGCATGGCGATTGGTGCCGGAACCGTGCTGATGCCAGTACAAATTGGCTTGAAGGGAATTTGGGTATTTATTACCGCAGCGATCATTGCTTATCCTGCCACCTGGGTAGTGCAGGACATTTATTTAAAAACCCTTTCTGAAAGCGATTCCTGTAATGACTACACCGATATTATCAGTCATTACCTGGGGAAGAACTGGGGAATTTTCCTCGGGGTTATC  >  NC_000913/3700589‑3700862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑