| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,132,787 | 0 | A | C | 98.3% | 180.8 / NA | 60 | I204S (ATC→AGC) | wcaA | putative glycosyl transferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (34/25); minor base T (0/1); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.33e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132653‑2132926 | ggTAGAGGTTAACCTGCTGTTATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCTGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc < 2:889644/143‑1 (MQ=255)ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGGCGAATTTGTCTTTGTGCTTGCGGTATAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGAGATCTGCAGTTCACCGTGATTGATATGCAGGCGCTGCGTc > 2:413632/1‑143 (MQ=255) gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAACAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCg > 2:1075683/1‑143 (MQ=255) gagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCct < 1:952237/143‑1 (MQ=255) ggTAAGCAGCTGATATTTTTTGCGGGCGCGGTCGTATTTGTCTTTGTGCGTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt < 2:796826/143‑1 (MQ=255) ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTCTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACTAGGTCATCTGCGTTTCACCGCGATTGATATGCAGGCTCTGCGTCGCCcctt > 2:1081763/1‑143 (MQ=255) tAAACAGCTGATATTTTTTTGTGGGGCGGTCGAATTTGTCGTTGTGCTTGTGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttct < 2:437597/143‑1 (MQ=255) tAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttct > 1:779877/1‑143 (MQ=255) aaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAc > 2:1118878/1‑143 (MQ=255) ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgcgt > 2:957841/1‑140 (MQ=255) ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt < 2:717850/141‑1 (MQ=255) ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt > 1:717850/1‑141 (MQ=255) cgcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGCCTTCTTCCCGCAACTAGTTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc < 1:1156221/143‑1 (MQ=255) tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCGCGGCGTCGCCCCTTCTACTCTCCACCGTCCGCCGTACTccgcccc > 2:210463/1‑141 (MQ=255) tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTTGTATGCAGGCTCTGGGTCGCCTCTTCTACTTTCCACCGTTTGCCGGCCTCCTGCACCAccccc > 2:809278/1‑141 (MQ=255) gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa < 1:1045467/143‑1 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGGAggg > 1:327787/1‑142 (MQ=255) tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTCATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAggc > 2:95190/1‑142 (MQ=255) ttGTTCTTCCGATGAAAGCGGGAGTACCCGGTGGATTTTTTCGGGGACGGGGTGATCTGCATTTCACCGTGACTGACATGCAGGCTCTGCGTCGCCCCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAggca < 2:533740/143‑3 (MQ=255) gtgCTTGCGGTAAAAGTGGAAGTACCCGGAGCATTTTTTCGGTGACGAGGTGATCTGCATTTGACCGTGATTGATATGCAGGCTCTGCGTCGCATCTTCTACTTTCCACGGTTGGCCGTACTGCACCACCATCCCCAGGAAGa > 1:510603/1‑143 (MQ=255) tgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAt < 1:664240/143‑1 (MQ=255) cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGt < 1:344022/143‑1 (MQ=255) ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTc < 1:678576/143‑1 (MQ=255) gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGCCGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTGCTCCGCCGCCATCCCCGGGCAGATGTCGt > 1:627927/1‑143 (MQ=255) gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGt > 1:646607/1‑143 (MQ=255) cGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCCGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGCTGTCGtc > 2:856810/1‑142 (MQ=255) ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa > 2:207835/1‑143 (MQ=255) tAAAGGTGGAAGTACCCGGAGAAGTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc < 2:1048460/143‑1 (MQ=255) aaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGGCCTCCACCACCACCCGCAGCACGAcgtcgtcctcc > 2:535065/1‑138 (MQ=255) aaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCt > 1:989836/1‑143 (MQ=255) tcggccccGGAGAATTCTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc < 2:204397/138‑1 (MQ=255) aaGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc > 1:2774/1‑143 (MQ=255) aaGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc > 1:505407/1‑143 (MQ=255) aaGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCACCAAAGTGAATATCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc > 1:1154723/1‑143 (MQ=255) gaggATTTTTTCGGTGACGAGGTGATCTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc < 2:983810/143‑1 (MQ=255) tgagttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGTTATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 2:73695/139‑1 (MQ=255) agaCTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACTGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg < 1:64214/143‑1 (MQ=255) ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt > 1:399070/1‑143 (MQ=255) tgtttCGGTGATGAGGTTATCTGCTTTTCACTGTGATTGTTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCTCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc < 2:160084/141‑1 (MQ=255) gACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca > 1:764281/1‑143 (MQ=255) gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCTTGTGCCGCTTTCAGTTCGGTATCGAACCAACActc > 1:1092300/1‑143 (MQ=255) aGGTGGTCTGCATTTCACCGTGATTGATATGCACGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActct < 2:187803/143‑1 (MQ=255) ggtggtCTGCTTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt < 2:749689/143‑1 (MQ=255) gTGCTCTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt < 2:913280/143‑1 (MQ=255) gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTTCCTGCTTGTGATCTCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt < 1:266096/143‑1 (MQ=255) aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTCTCCCGCTTTCAGCTCGGTATCGAACAAACACTCTCTGac > 2:225120/1‑142 (MQ=255) tCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATGCTGCGCCGCTTTCAGTCCGGTAGCCAACAACCACACTTTGaaa > 2:490111/1‑143 (MQ=255) cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAGTCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAc > 1:846176/1‑143 (MQ=255) tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt < 2:980333/49‑1 (MQ=255) tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt > 1:980333/1‑49 (MQ=255) cATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGCATCGACCAAACACTCTTTGAAACGcc > 2:580946/1‑143 (MQ=255) tttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCGACCACCCTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAAAAAACACGCGTTGAAACGCCAt > 2:1063379/1‑143 (MQ=255) ttgcgccGTGATTTATTTGCAGGTTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCTTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCAt < 2:118037/138‑1 (MQ=255) aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCGGTTCGGTATCGAACAAACACTCTTTGAACCGCCATGccc > 1:90601/1‑143 (MQ=255) cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg > 1:816421/1‑143 (MQ=255) tcgatGTGCACGCTCTGGATCTCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa < 2:1063046/141‑1 (MQ=255) tgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGGAGATGTCGAAATCCTGTGCCGCCTCCAGTCCGGTATCGAACAAACACTCTTTGACACGCCATGCCCAGGTACAg > 1:1121462/1‑143 (MQ=255) atGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACGCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt < 1:200902/143‑1 (MQ=255) cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGCAAGAGGGCGTAGTCCTGTGCCGCTCTCAGTTCGGGATCGAACAAACACCATTTGAAACGCCAGGCCCCGGGACAGACATGGt > 2:141523/1‑143 (MQ=255) aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt < 1:809278/143‑1 (MQ=255) | GGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT > NC_000913/2132653‑2132926 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |