Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,361,5350AT20.9% 78.4 / 12.6 44I226N (ATC→AAC) rhmRputative DNA‑binding transcriptional regulator for the rhm operon
Reads supporting (aligned to +/- strand):  ref base A (16/18);  new base T (0/9);  total (17/27)
Fisher's exact test for biased strand distribution p-value = 1.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.02e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTC  >  NC_000913/2361395‑2361677
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ttAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGAtt                                                                                                                                                <  2:901160/143‑1 (MQ=255)
 tAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGAttt                                                                                                                                               >  1:1115494/1‑143 (MQ=255)
            aGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTa                                                                                                                                    <  1:139120/143‑1 (MQ=255)
                 cATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCCGAGGGGCTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGac                                                                                                                             <  1:566195/143‑1 (MQ=255)
                 cATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGac                                                                                                                             <  1:111929/143‑1 (MQ=255)
                 cATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGac                                                                                                                             <  1:556230/143‑1 (MQ=255)
                  atgatgTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCACTGGAGCAGGCAATGGCCTTGCCCGCCAGATACTCACGGGCATCTGCATGGCTTTGTACGCGAGTAC‑‑CGacg                                                                                                                            >  2:539074/1‑142 (MQ=255)
                    gatgTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGaccac                                                                                                                          <  1:1023918/143‑1 (MQ=255)
                          ctcCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGAt                                                                                                                    <  1:388659/143‑1 (MQ=255)
                            ccATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCg                                                                                                                  >  1:523489/1‑143 (MQ=255)
                            ccATTGTTATGAGGCTTGTAAGTCAAAGCGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCg                                                                                                                  <  1:893411/143‑1 (MQ=255)
                             cATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGc                                                                                                                 >  2:1118246/1‑143 (MQ=255)
                                tgtgATGAGGGTTGTAAGTCAAAGGGACTTTTCCATCCCAACGGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCTTTTTTTTTTAATGATACGG‑‑CGACCACCGAGATCGCAGc                                                                                                              <  2:808797/143‑1 (MQ=255)
                                      gAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTGCATTGATGCGTAAACGGGTAC‑‑TGACCACCGAGATCGCAGCGGTAAg                                                                                                        >  1:150511/1‑143 (MQ=255)
                                           ttGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTCAGCTCgc                                                                                                   >  1:17290/1‑142 (MQ=255)
                                            tgcacgTCAAATGGTCTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCATTTTATGGCCTTGCCCGCCGGATACTCACGGTATTCTTCTTTTTTTTGTAA‑‑TGATACGGCGACCACCGAGATCGCAGCGGTAAGCTCGtt                                                                                                  <  2:810798/138‑1 (MQ=255)
                                              tAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCTGCTGGTTAATCCAGGTCTTCTTCATTTATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTAt                                                                                                <  1:819410/143‑1 (MQ=255)
                                               aaGTCAAAGGGACCTCTCCGTCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTAtt                                                                                               <  1:692845/143‑1 (MQ=255)
                                                aGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCCCGTTATTg                                                                                              >  1:604160/1‑143 (MQ=255)
                                                  tCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCCCCGACATCGCCCCGGTAAGCTCCTTATTGGc                                                                                            >  1:1114965/1‑143 (MQ=255)
                                                    tgctgttcTTTTCCATCCCTACATGCGTGAAGTCTCCGTGGGGCAGGTTATGGTTTTGCCCGCCAGATCATCACGGTGTGCTTCTTTTTTTTTTAA‑‑TGATACGGCGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGt                                                                                          <  2:861693/136‑1 (MQ=255)
                                                    aaaGGTACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGt                                                                                          <  1:398127/143‑1 (MQ=255)
                                                      aGGGACTTTTCCACCCCAACAGACGTGAAACATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTa                                                                                        <  1:593292/143‑1 (MQ=255)
                                                       ggtggttttCCAGCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCATTATTTGTTAGATAGTCACGGTATTCTTCTTTTTTTTTTAATGATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTaa                                                                                       <  2:389599/138‑1 (MQ=255)
                                                       ggtaCTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCTTTGTTTTTTAATGATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTaa                                                                                       <  2:197413/140‑1 (MQ=255)
                                                            ttttCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACAcc                                                                                  >  1:899118/1‑143 (MQ=255)
                                                                     ccAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGCGATCGCCGCGGCAAGCTCGTTATTGGCGCTCCACCCCGGCGCAGCg                                                                         >  1:835092/1‑143 (MQ=255)
                                                                      taaCTGAGGTGCAATAGCGCTAGCGCTGGCCATTGCCTTGCCCGCGAGGTAGTCACGGTATTGTTCTTTTTTTTTTAATCATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGa                                                                        <  2:746095/142‑1 (MQ=255)
                                                                               tGAAATATCCCTGGCGCAGGCAATGGCCTTGACCGCTGTTAATTCTCTTTTTTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACGCAACGGa                                                               >  1:768127/1‑143 (MQ=255)
                                                                                           tgcgcAGGCATTGTTCTTGCCCGGCAGCTAGTCACGGTATTGTTCTTTTTTTTTTAAGGATGCGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAAt                                                   <  2:710322/142‑1 (MQ=255)
                                                                                                 ggCGATGGGCTTGCCAGCCAGAGAATGACGGTATTCTTCGTTGATTTGGAAGGGGGCGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGc                                             <  2:1027924/143‑1 (MQ=255)
                                                                                                   cAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCg                                           >  1:714404/1‑143 (MQ=255)
                                                                                                    aaTGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACCACGGGCAGCGGCGGGATCGTCGcccg                                          >  1:132260/1‑141 (MQ=255)
                                                                                                          cTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCg                                                                                           >  2:158367/1‑88 (MQ=255)
                                                                                                          cTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCg                                                                                           <  1:158367/88‑1 (MQ=255)
                                                                                                          cTTGCCAACCAGATAATCACGGTATTTTTCATTTTTTTTTAATGATACGG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCAt                                    <  2:464014/143‑1 (MQ=255)
                                                                                                               ccGCCAGCTAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTc                               <  1:356295/143‑1 (MQ=255)
                                                                                                               ccGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTc                               <  1:582892/143‑1 (MQ=255)
                                                                                                                   cAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCCTTAAACACCGGCGAACAGACACAACGGACATCTGCGTAACCTCCGCCGATGGCAAAgacacagc                           >  2:902389/1‑136 (MQ=255)
                                                                                                                    aGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACCTCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGcc                          >  1:500412/1‑143 (MQ=255)
                                                                                                                                 tATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGCCGTTAAACACCGCCGCAGCGACACAAGGGACAGCGGCGTAATCTTCGCCGCTGTCCTAGCTTCAGCCCTGGCGGCGGAt              >  2:240786/1‑143 (MQ=255)
                                                                                                                                     tctttttGTTTTGTAAGGAGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATAcgc          <  2:22334/138‑1 (MQ=255)
                                                                                                                                     cttcttTGATTTGTAAGCGTGTAG‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATAcgc          <  2:150511/143‑1 (MQ=255)
                                                                                                                                       tcATTGATCTGTAAGCGGGTAC‑‑CGACCACCGAGCTCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACgcgc        <  1:142868/143‑1 (MQ=255)
                                                                                                                                             aTTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTGGTAGCTCCAGCGCTGGCGGCGAATAGGGGCCAGttc  >  1:914603/1‑143 (MQ=255)
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TTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGGGACTTTTCCATCCCAACAGACGTGAAATATCCCTGGCGCAGGCAATGGCCTTGCCCGCCAGATAATCACGGTATTCTTCATTGATTTGTAAGCGGGTAC‑‑CGACCACCGAGATCGCAGCGGTAAGCTCGTTATTGGCGTTAAACACCGGCGCAGCGACACAACGGACATCGGCGTAATCTTCGCCGTTGTCATAGCTCCAGCCCTGACGGCGAATACGCGCCAGTTC  >  NC_000913/2361395‑2361677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: