Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (‑2/+1)	glpR ← / ← glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	134.2 / NA	41	intergenic (‑2/+1)	glpR/glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (17/24);  total (17/24)

GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTT  >  NC_000913/3560316‑3560584                                                                                                                                             |                                                                                                                                  gATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCCGCGCCGCACCGCCACGATGGcgca                                                                                                                                 >  2:119387/1‑140 (MQ=255)         gCGTCGAGCCATTGGGGATTTGCTCCCCCACCTTGCGGGCGATGCGCTCTTTTTCTCCGGTCTGGGTGGCTCTGCGATCGTGCCACGGCTTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAgg                                                                                                                         <  1:278331/143‑1 (MQ=255)              gggCCATTGGGTATTTGCTCCGCCACGTTGCGGGCGATGCGCTCTCTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                    <  2:73159/141‑1 (MQ=255)              gAGTCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAAGGCGCAGGATCAGGTTTTg                                                                                                                    <  1:232430/143‑1 (MQ=255)              gAGCCAGTGGGCATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTCTTTCGTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                    <  2:191470/143‑1 (MQ=255)               aGCCAGTGGGGATTTGCTGCGCCACTGTGCGGGCGAGGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGc                                                                                                                   <  2:436471/143‑1 (MQ=255)                 cgaTTGGGGGTTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTGCTTCGGTCTGGGTGTCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTc                                                                                                                 <  2:794082/141‑1 (MQ=255)                 ccATTGGGGATTTGCGCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCTGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTc                                                                                                                 <  1:549380/143‑1 (MQ=255)                         ggtgtGCGCCGCCTCTTTGCGGGCGCTGCGTTCGTTTTCTTCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCt                                                                                                         <  2:686706/139‑1 (MQ=255)                           ggcgttCCGCCGCTCTGCGTGCGAGGCGCTCTGTTTCTTCGGTCGGGGTGGCCGTGCGATCGTGCCGCGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACCGCCGTGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCa                                                                                                       <  2:534220/138‑1 (MQ=255)                             tGCTCCGCCACCGTGCGGGCGATGCGCTCTGTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCCCCGCACCGCCATGATGGCGCAGGATCAGGTTTGGCTCCGCCAGCTCAtt                                                                                                     <  1:151688/143‑1 (MQ=255)                                tCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCTGGCGCAGGATCAGGGTTTGCTCCGCCAGCTCATTGAg                                                                                                  >  1:196128/1‑143 (MQ=255)                                  cGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGCGTGGCCTTGCGAGCGTGCCACGGCGGGTTCACCGAGCTGGCAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCCGGGTGTGCTCCGCCAGCTCAGTGAggc                                                                                                >  2:850812/1‑142 (MQ=255)                                       gttggccggCGATGTGCTCGTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                                                                                           <  2:412998/136‑1 (MQ=255)                                          tGCGGGCGATGCGCTCTTTTTCTTCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGGAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGa                                                                                        <  2:37465/143‑1 (MQ=255)                                           gCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                                                       <  1:655974/143‑1 (MQ=255)                                            cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTGTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                      >  1:426843/1‑143 (MQ=255)                                                        tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCt                                                                          >  1:7129/1‑143 (MQ=255)                                                           ttttCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                       <  1:894298/143‑1 (MQ=255)                                                           gtttCGTCGCTCTCGGCGCCCTTGCGATCGTGGCACGGCGTGTTAACCCAACTGGAAGGCAGCGCCGCACCGCCACGATTGCGCATGATCAGGTTTTGCTCCGCCAGCTCATTGATCTCGCGGCTAATAGTCTGCGGGCTGAc                                                                       <  2:821011/142‑1 (MQ=255)                                                            tttCTTCGGGCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACg                                                                      <  2:2625/143‑1 (MQ=255)                                                                 tcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                                                                 >  2:108387/1‑143 (MQ=255)                                                                 tcGGTCTGCGTGGCCGTGCGATCGCGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAGGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaa                                                                 <  2:347578/143‑1 (MQ=255)                                                                     tCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGACATGc                                                             >  1:320089/1‑143 (MQ=255)                                                                                   cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                               <  1:371069/143‑1 (MQ=255)                                                                                    gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                              <  1:136278/143‑1 (MQ=255)                                                                                    gATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCACCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg                                              >  1:592273/1‑143 (MQ=255)                                                                                    gATCGTGCCACGGCGTGGTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTATGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCCGACGGAGAAATGCTCAACCAGATCTACg                                              >  2:205927/1‑143 (MQ=255)                                                                                       cgggCCGCGGGGTGTGAACCGAACTGGAGGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                           <  2:725415/140‑1 (MQ=255)                                                                                           ccACGGCGTGTTAACCGAACTGGAAGGCTGGGCCGCATCGCCATGATAGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                       <  1:567585/143‑1 (MQ=255)                                                                                                gCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAtca                                  >  1:366160/1‑141 (MQ=255)                                                                                                    gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTGATCGGTTCCAACGTAACCct                              >  2:97051/1‑143 (MQ=255)                                                                                                     ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAGCAGGTTTTGCTCCGCCAGCGCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGAAGGAGAAATGCTCTACCAGGCCTTCGGTACTGACACAAcccgg                             >  2:823058/1‑141 (MQ=255)                                                                                                       aaCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCCGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctggc                           >  2:306167/1‑141 (MQ=255)                                                                                                         ccGAGCGGGAAGGCAGCGCCGCGCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTCCCAGCTCTTCGGTACTGACATAACCCTGCTGt                         <  1:299509/143‑1 (MQ=255)                                                                                                         ccGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTCCTGACATAACCCTGCTGt                         >  2:170486/1‑143 (MQ=255)                                                                                                           gAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                       >  1:177117/1‑143 (MQ=255)                                                                                                           gAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                       >  1:411395/1‑143 (MQ=255)                                                                                                               tGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATACCCCTGCTGTTTAAcc                   >  1:710366/1‑143 (MQ=255)                                                                                                                   aGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt               <  1:777694/143‑1 (MQ=255)                                                                                                                             gcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGCTACTAcc     >  2:83683/1‑143 (MQ=255)                                                                                                                                ccGCCCTGATGGCGCAGGATCAGGTTGTGCGCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGtt  <  2:215796/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                  GATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTT  >  NC_000913/3560316‑3560584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑