Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	0 (0.000)	82 (1.610)	58/284	0.0	100%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	0 (0.000)	73 (1.430)	43/284	0.2	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGT  >  NC_000913/2101609‑2101882                                                                                                                                             |                                                                                                                                      ttCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaagga                                                                                                                                     <  1:809836/143‑1 (MQ=255)   cacaGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaaggaag                                                                                                                                   <  2:553173/143‑1 (MQ=255)    acaGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaaggaagg                                                                                                                                  >  2:186374/1‑143 (MQ=255)       gTATATAAAGTAACCTTGATCGAGACAATTGACACGCAGAAAATCTGAAAAACGTAACAGCATAAATGATTCTGCGCACCAATCAACAATCGTATCAGAATAGATATTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGc                                                                                                                               <  2:724451/143‑1 (MQ=255)        tataTAAAATAACAATGATCGAAGCCATTAACACGAAAAAAATCTCAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCTTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCg                                                                                                                              <  2:618382/140‑1 (MQ=255)         aCATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAACCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGa                                                                                                                             <  1:295707/143‑1 (MQ=255)           aTAAAGTAACCTTGCTCGACGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAAt                                                                                                                           <  2:679991/143‑1 (MQ=255)                     cTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATGTTTGTTTTATTAACGAAGGCGCTAATAAACGGGGaa                                                                                                                 >  2:499327/1‑143 (MQ=255)                      ttGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGaaa                                                                                                                <  2:597606/143‑1 (MQ=255)                      ttGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATACTGCGCACCAATCAACAACCGTGTCAGAATAGATACTTTCCTTAGGAATTTCTGGTTTATTAAGGAAGGTGCGAATAAGCGGGGaaa                                                                                                                >  2:736090/1‑143 (MQ=255)                          tCGAAGCCATTTACACGCACAAAAACTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTGTCTATAGGAATATGTGTTTGATTAAGGCAGGTGCGCATAAGCGGGGAAAttct                                                                                                            >  1:390879/1‑143 (MQ=255)                                ccATTTCCACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCgg                                                                                                      <  1:118572/143‑1 (MQ=255)                                ccATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGAGAATAAGCGGGGAAATTCTTCTCgg                                                                                                      >  2:418336/1‑143 (MQ=255)                                    ttACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTAACAGTATAGATTCGTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGa                                                                                                  <  2:275989/143‑1 (MQ=255)                                          gCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGt                                                                                            >  1:238469/1‑143 (MQ=255)                                                 aTCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttca                                                                                     >  1:823351/1‑143 (MQ=255)                                                      aaaaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTCAGAACGGTGCCAGTAATCGGGGGAACCCCTTTGTGGTGGCTCAGTCATTTCATttct                                                                                >  1:125384/1‑143 (MQ=255)                                                      aaaaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttct                                                                                <  1:186374/143‑1 (MQ=255)                                                       aaaaCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGCAATTTTTGATTTATGAAAGAATGTTCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                               <  1:865041/143‑1 (MQ=37)                                                            gaaCCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGATTAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGt                                                                          <  1:864172/141‑1 (MQ=255)                                                             tACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGtt                                                                         <  2:211808/143‑1 (MQ=255)                                                              aCCAGCATAAATGATCCTGCGCACCAATCACAATCCGTCTCATAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGttt                                                                        <  2:189116/143‑1 (MQ=25)                                                               ccAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTg                                                                       <  1:787437/143‑1 (MQ=255)                                                                cAGCATAAATGATGCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                      <  1:709599/143‑1 (MQ=255)                                                                cAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                      >  2:575141/1‑143 (MQ=255)                                                                     tAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGa                                                                 <  2:44564/143‑1 (MQ=255)                                                                     tAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTCTCCCCCGAATTATTCGTTTTATAAAGGTGGGTACGAATAAGCGAGGGAAGTCTTCTCGGCGGACTCAGTCATTCTATTTCTTCATGTTTGAGCCGa                                                                 >  2:889525/1‑143 (MQ=16)                                                                      aaaTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGCAATTTTTGTTTTATTAAGGAAGGTGCGAACAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAt                                                                >  2:449699/1‑143 (MQ=255)                                                                          gATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTGTCTTTAGGAGTTTTTGTTTTATGAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAttttt                                                            <  2:735632/143‑1 (MQ=255)                                                                                  ccACCAAGTAACAACTGTATCAGAATATATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGt                                                    <  2:81104/142‑1 (MQ=255)                                                                                     cataTCAACATACGTAGCAGAATAGATACTTTCTTTAGGAAGTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTaaa                                                 <  2:28126/140‑1 (MQ=18)                                                                                      cAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAAt                                                >  1:768544/1‑143 (MQ=255)                                                                                      cAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGCGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAAt                                                >  2:244345/1‑143 (MQ=255)                                                                                           gacaatCGGATCATAATAGATACGTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCtt                                           <  2:385444/142‑1 (MQ=255)                                                                                             cagccGTATCAGAATAGATACCTTCTGTAGTAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGa                                         <  1:526240/140‑1 (MQ=255)                                                                                              aacCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                        >  2:436494/1‑143 (MQ=255)                                                                                              aacCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                        >  1:99483/1‑143 (MQ=255)                                                                                                   tATCAGAATAGGTACTTTCTTGAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                   <  1:410571/143‑1 (MQ=255)                                                                                                        gAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCTGCTTACTCAGTCATTTCATTTCTTGTTGTTTGAGCCCACTTTTTCTCCTGTAAATTTCTTGTATTAGCCtttt                              >  2:885331/1‑143 (MQ=18)                                                                                                        gAATAGATACTTTCTTGAGGAATTTTTGTTTTCTTAAGGAAGGGGCTAATAAGCGGGGAAGTTATTGTGTGCTGACCCACAAACTTGCGAAATTCCGGTTGGAGCCGATTGTTTCTCCCGTAAATGCCTTGAATCAGCCTAtt                              <  1:424862/143‑1 (MQ=12)                                                                                                            cgctcctttctttAGGAATTTTTGTTTTCTTAAGGAAGGTGCGCATAAGCGGCGACATTATTTTCGGCTGGCTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTTCTCCCGGAAATGCCTTGAATCAGCCTATTTAGa                          <  2:447920/138‑1 (MQ=17)                                                                                                                aCTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTATTCTCGGCTGACTCAGTCACACCATTCCTACACGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGt                      >  1:155790/1‑143 (MQ=17)                                                                                                                  tttctttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGATTCAGCCTATTTAGACCGttt                    >  2:824886/1‑143 (MQ=255)                                                                                                                      tttAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTTAATCAGCCTATTTAGACCGTttcttc                <  2:339181/143‑1 (MQ=255)                                                                                                                      tttAGGAATTTTGGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATATGTTTTCTTCTTGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttcttc                <  1:573302/143‑1 (MQ=14)                                                                                                                        tAGGAATTGTTGTTTTATTAAGGGATGTGATGATAAGCGGGGAGATTCTTCTCGGCTTACTCAGTCATTTCATGTCTTCATGTTTGAGCCGAGTTTTGCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGc              <  2:900555/143‑1 (MQ=14)                                                                                                                         aGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGcc             >  1:855103/1‑143 (MQ=255)                                                                                                                         aGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCGATTTAGACCGTTTCTTCGcc             >  1:46199/1‑143 (MQ=255)                                                                                                                         aGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTATTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGCTTCTTAGcc             >  2:183954/1‑143 (MQ=255)                                                                                                                         aGGAATTTTTGTTTTATTAAGGAAGGTCCGAATAAGCGAGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGcc             <  2:733972/143‑1 (MQ=255)                                                                                                                          ggAGTTTTTGTTTTATTAATGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCAAGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCa            <  2:483440/143‑1 (MQ=255)                                                                                                                                  tGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGc    <  2:861602/143‑1 (MQ=35)                                                                                                                                  tGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCGTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGc    <  2:450906/143‑1 (MQ=25)                                                                                                                                    ttttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCAATTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGACCGTTTCTTCGCCTTTTAAGGCGt  >  1:148755/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                      TTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGT  >  NC_000913/2101609‑2101882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.