Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	74 [1]	[0] 72	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	1 (0.020)	70 (1.410)	59/276	0.0	99.3%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

AAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC  >  NC_000913/1299356‑1299638                                                                                                                                               |                                                                                                                                             aaaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAA                                                                                                                                              >  1:578475‑M1/1‑143 (MQ=255)  aaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                                                                                                             >  2:557701‑M1/1‑142 (MQ=255)    ttACAGTTAGTAATAGAGATTTCCTCATCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAata                                                                                                                                           <  2:314917‑M1/143‑4 (MQ=255)    tgccagtTAGTGATCAGTAGTTCCTTAACTGCTTCTCCCCCCCATCATGTTATTTTCGCCACATCATAATCTTGGGCTTGCTGAAGAATAATTGAAATAATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAata                                                                                                                                           <  1:730363‑M1/140‑4 (MQ=255)        agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                                                                                                                       <  2:538332‑M1/143‑8 (MQ=255)          ttagttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatc                                                                                                                                     >  1:508473‑M1/1‑134 (MQ=255)              ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                 <  1:214924‑M1/143‑14 (MQ=255)                 taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                              >  2:827463‑M1/1‑127 (MQ=255)                   agcatttCCTTAACTGCTTCCCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaa                                                                                                                            <  1:855420‑M1/140‑19 (MQ=255)                     gATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATACTTGAAATGATATTCTTCGCAGGACGACCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                                                                                                                          >  1:383959‑M1/1‑123 (MQ=255)                     gATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaat                                                                                                                          >  1:740880‑M1/1‑123 (MQ=255)                      aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatt                                                                                                                         >  2:547461‑M1/1‑122 (MQ=255)                       tttCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatta                                                                                                                        >  1:223954‑M1/1‑121 (MQ=255)                           cTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgt                                                                                                                    <  1:872846‑M1/143‑27 (MQ=255)                            ttAACTGCTTCTCCTCACCTTCATCTTATGTTCGCCACCTCCTGTTCCTGTGCGTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                   <  2:786510‑M1/143‑28 (MQ=255)                            ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                   <  1:759808‑M1/143‑28 (MQ=255)                            ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCAAATCGTTATCCTTGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGCGGAAAGTGCTAAataatactcaatggttaaattattgta                                                                                                                   >  2:500283‑M1/1‑116 (MQ=255)                              aaCTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtaca                                                                                                                 <  1:427603‑M1/143‑30 (MQ=255)                                  ttttctCTTCACAATGATGTAAAATGCGACAGAACATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttc                                                                                                             <  1:52705‑M1/141‑34 (MQ=255)                                   cTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                            <  1:895647‑M1/143‑35 (MQ=255)                                         ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                      <  2:508474‑M1/143‑41 (MQ=255)                                         ctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactg                                                                                                      >  2:533863‑M1/1‑103 (MQ=255)                                           cACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactgga                                                                                                    >  2:848756‑M1/1‑101 (MQ=255)                                            aCCTTCATGTTAGTTGCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAAGGATAGTATTAATTCCACTGCCTTTGGTATAGGAAAGTGCTAAatgataatcaattgttaaattattgtgcatttcactactggaa                                                                                                   <  2:600981‑M1/143‑44 (MQ=255)                                            aCCATCATGTTATTTACGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                   >  2:770619‑M1/1‑100 (MQ=255)                                                 cctgtTATTTTCGCAACATCATAATCCTGTGCTTGCTGAATAATAATTGAAAAGATATTATTAATTCCACTGCTTTTGGTAGATGAAAGTGCTAAataataattaattgttaaattattgtgcatttcactactggaactgta                                                                                              <  2:874829‑M1/141‑49 (MQ=255)                                                   tattatTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                            <  1:331655‑M1/141‑51 (MQ=255)                                                   tGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGCGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaat                                                                                            >  2:842682‑M1/1‑93 (MQ=255)                                                    gTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatc                                                                                           >  2:754314‑M1/1‑92 (MQ=255)                                                        ttttCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaa                                                                                       >  2:201546‑M1/1‑88 (MQ=255)                                                             gCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcctttcactactggaactgtaataagaaaagaa                                                                                  >  1:290766‑M1/1‑83 (MQ=255)                                                              ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                 <  1:615160‑M1/143‑62 (MQ=255)                                                              ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                                 <  1:490068‑M1/143‑62 (MQ=255)                                                               cacaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatag                                                                                <  2:383399‑M1/143‑63 (MQ=255)                                                               cacaTCATAAGCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGTTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatag                                                                                <  2:246979‑M1/143‑63 (MQ=255)                                                                acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTTGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaacgataga                                                                               >  1:710667‑M1/1‑80 (MQ=255)                                                                 catcatAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcattccactactggaattgtaatcagaaaagatagac                                                                              <  2:800729‑M1/143‑65 (MQ=255)                                                                  atcatAATCCTGGGTTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCGTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagaca                                                                             <  2:195763‑M1/143‑66 (MQ=255)                                                                    catAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatg                                                                           >  1:773076‑M1/1‑76 (MQ=255)                                                                         tCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttag                                                                      >  1:633185‑M1/1‑71 (MQ=255)                                                                          ccTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                     <  1:711208‑M1/143‑74 (MQ=255)                                                                                cTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatct                                                               >  2:242312‑M1/1‑64 (MQ=255)                                                                                cTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatct                                                               <  1:533863‑M1/143‑80 (MQ=255)                                                                                    cTGACGAATACTTGAGATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatggtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctat                                                           <  2:900688‑M1/143‑84 (MQ=255)                                                                                    cTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctat                                                           <  1:547461‑M1/143‑84 (MQ=255)                                                                                    cTGAAGAATAATTGAAATCTCCATATTGATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctat                                                           <  2:680360‑M1/143‑84 (MQ=255)                                                                                        agaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttga                                                       <  2:194494‑M1/143‑88 (MQ=255)                                                                                          aacaattgaAATGATATTATTGATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgatt                                                     <  2:578676‑M1/140‑90 (MQ=255)                                                                                               ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                >  2:363634‑M1/1‑49 (MQ=255)                                                                                               ctgAAATCATATTATTAATTCCACTGCTTTGGGTAGAGGAAAGTGCTAAataataatcaattgttcaatgatggtgcagtgcacgactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                                <  2:465719‑M1/142‑95 (MQ=255)                                                                                                      gATATTATTAATTCCTCTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagat                                         <  2:295622‑M1/143‑102 (MQ=255)                                                                                                          ttattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaagtgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                     <  1:349460‑M1/143‑106 (MQ=255)                                                                                                             gtaaTTACACTGGCTTTGGTAGAGGAGAGTGCTAAataataataaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatgtctatttgattgaattgaaagatgtttgtt                                  <  2:425827‑M1/142‑109 (MQ=255)                                                                                                                 ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaagagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                              >  1:737351‑M1/1‑31 (MQ=255)                                                                                                                 ttCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                              >  2:534853‑M1/1‑31 (MQ=255)                                                                                                                     acggcgtttgttggaGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatg                          <  2:567981‑M1/137‑117 (MQ=255)                                                                                                                      cTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgg                         <  1:242312‑M1/143‑118 (MQ=255)                                                                                                                        gCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                       <  2:167928‑M1/143‑120 (MQ=255)                                                                                                                          cTTTGGTAGAGGAGAGTACTAAataataagtaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgc                     <  2:314974‑M1/143‑122 (MQ=255)                                                                                                                           tttGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagctagccatgctcagccaatctcgatgtgattgaattgaaagatgcatgttcgtgcatggctgca                    >  2:185789‑M1/1‑21 (MQ=255)                                                                                                                              ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagc                 <  2:383959‑M1/143‑126 (MQ=255)                                                                                                                               gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagct                >  2:2071‑M1/1‑17 (MQ=255)                                                                                                                               gTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagct                >  1:619715‑M1/1‑17 (MQ=255)                                                                                                                                tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagcta               >  1:396820‑M1/1‑16 (MQ=255)                                                                                                                                  ggaggaaGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATACAACAGGGATCCTCATGAGTCATCAACTTACCTTCGCCGACAGTGCATTCACCACTAAGCGCCGTCAGACCACaa             >  2:874697/6‑143 (MQ=11)                                                                                                                                  gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctata             <  2:578475‑M1/143‑130 (MQ=255)                                                                                                                                  gagGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagacatggatgcaagctata             <  1:113238‑M1/143‑130 (MQ=255)                                                                                                                                     gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagat          >  2:597231‑M1/1‑11 (MQ=255)                                                                                                                                        aGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattct       >  2:899806‑M1/1‑8 (MQ=255)                                                                                                                                          tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctga     >  1:16973‑M1/1‑6 (MQ=255)                                                                                                                                          tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctga     >  2:332768‑M1/1‑6 (MQ=255)                                                                                                                                           gCTAAataagaagcaattgttaaattactgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgat    <  1:629658‑M1/143‑139 (MQ=255)                                                                                                                                            cTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttggttgaattgaaagatgtttgttaaggcatggatgcaagctatagattctggtc   >  2:507080‑M1/1‑4 (MQ=255)                                                                                                                                             tAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatac  >  2:856740‑M1/1‑3 (MQ=255)                                                                                                                                               |                                                                                                                                             AAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTC  >  NC_000913/1299356‑1299638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑