Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,315,391	0	G	T	29.1%	70.8 / 25.9	56	H50N (CAT→AAT)	phnO	aminoalkylphosphonate N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (25/14);  new base T (11/5);  total (37/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ACTTACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAG  >  NC_000913/4315250‑4315531                                                                                                                                              |                                                                                                                                             gccgcgtGCCGACGTTCGGACCGCGGTCTTGCGGGCTTGGCACCGTGTCCGGGGTGGCGCCGATCCGGTTGACATGATGCAGATGAACCTGAAATTGCAGGAACATTATCGGCACTATGTCGCCATCAAGCAGCGCCAGATgg                                                                                                                                             <  2:749290/137‑1 (MQ=255)   ttACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATCTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTa                                                                                                                                           <  1:115120/143‑1 (MQ=255)    tACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTAg                                                                                                                                          <  1:759711/143‑1 (MQ=255)         ccGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCAt                                                                                                                                     >  1:532708/1‑143 (MQ=255)                  aGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGCTGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTc                                                                                                                            >  1:56973/1‑143 (MQ=255)                  aGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCCGCGCCAGATGGTAGCGCATGTTTgggcc                                                                                                                            >  1:349694/1‑141 (MQ=255)                            ccTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCACATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTACCGCATGTTTGGGTCGCGCCGCTTg                                                                                                                  >  2:648973/1‑143 (MQ=255)                             cTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCCGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTgg                                                                                                                 >  2:201525/1‑143 (MQ=255)                             cTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGACAGTTGGGCATGGTTGGGTGGCGAAgcatgg                                                                                                                 >  2:419568/1‑138 (MQ=255)                              tGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGc                                                                                                                >  2:886107/1‑143 (MQ=255)                                     ttACCACCCACTCCTGAAGTGCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTaaaa                                                                                                         <  2:626832/143‑1 (MQ=255)                                       accaccAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGAGGAAACTGCAAATGCAGGCCGATCATGCCGACAACATCGCCATAAAGCAGCGCCAGATGGAAGCGCAAGTAAGGGACACACAGAGAGGCGATACAAcc                                                                                                       >  2:744267/1‑143 (MQ=255)                                                  cTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAACTGCAGGCCGATCATGCCGACAGCTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAAcg                                                                                            >  2:459802/1‑143 (MQ=255)                                                  cTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAAcg                                                                                            >  2:794563/1‑143 (MQ=255)                                                       tttCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGACGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtgg                                                                                       <  1:622273/143‑1 (MQ=255)                                                        ttCGCCGACCCCGTCGACATGATGCAGATGAAACTGCAAATGCAGGCCGCTCATGCCGCCAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCgtggt                                                                                      <  2:585171/143‑1 (MQ=255)                                                           gCCGATCCAGTTGACATGATGCAGATGAAACTGCAACAGAAAGCCGATCATGACGACAACTTCGCCATCAAGCAGCGCCAGAAGGTAGCGCATGTTTGGGTCGCGCAGACTGGCGTGAAAACCCACGCGAAAGGCGTggtggt                                                                                   >  2:745673/1‑143 (MQ=255)                                                           gCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCGTCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTggtggt                                                                                   >  1:746121/1‑143 (MQ=255)                                                           gCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTggtggt                                                                                   >  1:24075/1‑143 (MQ=255)                                                                  cAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGGCCAACTc                                                                            >  2:236406/1‑143 (MQ=255)                                                                   aGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGAAAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTcc                                                                           <  1:440841/143‑1 (MQ=255)                                                                    gtggACGTGATGCAGCTGAAACTGCAAATGCAGGGCGATCACGCCGACGACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTGTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCg                                                                          <  1:147346/140‑1 (MQ=255)                                                                       gACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTTGCTTTAAAACCCAATCGAAAAGAGTAGTGGTAAAACTCCGCCt                                                                       >  1:521621/1‑143 (MQ=255)                                                                           tgatgCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGttt                                                                   >  1:782821/1‑143 (MQ=255)                                                                            gatgCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTCGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGtttt                                                                  >  1:187773/1‑143 (MQ=255)                                                                             gtgCAGATGAAACTGCAAATGCGGGCCGATCATGCCGACAACTTCGCCCTCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTa                                                                 <  2:575886/142‑1 (MQ=255)                                                                                cAGATGAGACTGCAAAGGCGGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCt                                                              <  2:271692/143‑1 (MQ=255)                                                                                cAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGAGGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCt                                                              <  1:31518/143‑1 (MQ=255)                                                                                 aGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTc                                                             <  1:795220/143‑1 (MQ=255)                                                                                       aaCTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAAt                                                       >  1:418892/1‑143 (MQ=255)                                                                                        aCTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGATCCGCAAGGTTGGGTAGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATc                                                      >  1:623041/1‑143 (MQ=255)                                                                                          tGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAg                                                    >  1:482782/1‑143 (MQ=255)                                                                                             aaaTGCGGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAgcgc                                                 <  2:649636/143‑1 (MQ=255)                                                                                                    ggCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAATACGCACTCGAGACGCGTGGTGGTCAACCTCCGCCTGTTTTAGCTCACCAGTCAGCGCGTAAccc                                          >  2:225767/1‑143 (MQ=255)                                                                                                     gCCGATCATGCCGTCAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACcg                                         >  1:169112/1‑143 (MQ=255)                                                                                                        gATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTGTTAGCTCACAAATGAGCGCGTAAGCagcga                                      >  2:533394/1‑142 (MQ=255)                                                                                                         aTCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGAGTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTACTTTAGCTCACAAATCAGCGCGTAAACagcggc                                     >  2:535289/1‑141 (MQ=255)                                                                                                          tCATGCCGACGAGTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCg                                    <  2:195860/143‑1 (MQ=255)                                                                                                          tCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATTGGAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCg                                    <  1:811423/143‑1 (MQ=255)                                                                                                                cGACAACTTCGCCATCAAGCCGCGCCAGATTGTAGCGCATGTCTGGGTCGCGCAGATTGGCGTTCAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCg                              <  1:745673/143‑1 (MQ=255)                                                                                                                cGACAACTTCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAGACTTCGCCTATTTTAGCTCACAAATCAGCGCGTAAACCGCGTAGGTGTCg                              >  2:439361/1‑143 (MQ=255)                                                                                                                cGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCg                              >  1:549711/1‑143 (MQ=255)                                                                                                                    aaCTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGACACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGAGTCGTACt                          >  1:231001/1‑143 (MQ=255)                                                                                                                       ttCGCCATCAAGCAGCGCCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAGAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTGTTAGCTCACAACTCAGCGCGTAAACCGCGTGGGGGTCGGACTggg                       >  2:487954/1‑141 (MQ=255)                                                                                                                           ccATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGc                   <  1:251891/143‑1 (MQ=255)                                                                                                                            cATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGcc                  >  1:471726/1‑143 (MQ=255)                                                                                                                                aaGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGc              >  1:878632/1‑143 (MQ=255)                                                                                                                                    accgcCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAg          <  1:441510/141‑1 (MQ=255)                                                                                                                                     gcgcCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGc         <  2:418892/143‑1 (MQ=255)                                                                                                                                      cgcCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt        >  2:860988/1‑143 (MQ=255)                                                                                                                                      cgcCAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCt        >  1:13056/1‑143 (MQ=255)                                                                                                                                       gacAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCAACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTCGGTCACAAATCAGCACGTAAACCGCGCAGGTGTCGTAATGCGTGGCCAGACGAAGcac       >  2:486873/3‑141 (MQ=255)                                                                                                                                        ccAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTcc      >  1:500006/1‑142 (MQ=255)                                                                                                                                        ccAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTca      <  1:378708/143‑1 (MQ=255)                                                                                                                                         cAGATTGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCCcac     >  2:123281/1‑143 (MQ=255)                                                                                                                                           gATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACaa   >  2:141757/1‑143 (MQ=255)                                                                                                                                            aTGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAg  >  2:118289/1‑143 (MQ=255)                                                                                                                                              |                                                                                                                                             ACTTACTGCCGACGTTCAGACCGCGCGCCTGCGGCATTACCACCAACTCCTGAATTTCGCCGATCCAGTTGACATGATGCAGATGAAACTGCAAATGCAGGCCGATCATGCCGACAACTTCGCCATCAAGCAGCGCCAGATGGTAGCGCATGTTTGGGTCGCGCAGATTGGCGTTAAAACCCACGCGAAACGCGTGGTGGTCAAACTCCGCCTGTTTTAGCTCACAAATCAGCGCGTAAACCGCGTCGGTGTCGTACTGCGTGGCCGGGCGAAGCTCACAAG  >  NC_000913/4315250‑4315531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑