Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,196,305 C→T R395C (CGT→TGT)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,3050CT98.3% 150.7 / NA 59R395C (CGT→TGT) icdisocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (38/19);  minor base G (0/1);  total (39/20)
Fisher's exact test for biased strand distribution p-value = 3.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTT  >  NC_000913/1196167‑1196444
                                                                                                                                          |                                                                                                                                           
gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACCTAATTGTTAAAGGTATGGAAGGCGCACTCAACGCGAAAACCGTACCCTATGACTCCGAGCGCCt                                                                                                                                         >  2:533535/1‑143 (MQ=25)
 acgaaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTGGAGTGCCTg                                                                                                                                        >  2:388633/4‑143 (MQ=255)
   aaaGTATTTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAATGCGAAAACCGTAACCTATGACTTCGAGTGTCTgat                                                                                                                                      <  2:153903/143‑1 (MQ=255)
   aaaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACGTCGAGTGTCTgat                                                                                                                                      >  1:699829/1‑143 (MQ=255)
   aaaGTAAATCCTGGCTCAATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGCCCGAAGCAGCCACCAAAACTGATAACGGAATGCATGGCGCAATCCACGCGAAAACCGTCACCTCTGACTTCGAGTGTCTgac                                                                                                                                      >  2:192873/1‑142 (MQ=255)
       tAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAGGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatggat                                                                                                                                  >  1:91875/1‑143 (MQ=255)
          aTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAGAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGgc                                                                                                                               >  1:539664/1‑143 (MQ=255)
                  nctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACAGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAAACAACGCGAANACCGTAACCTAGGACGTCGAGTGACTGAGGGGTGGCGCTaaaaa                                                                                                                       >  2:104849/2‑141 (MQ=21)
                       tattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAAGTATTGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGa                                                                                                                  <  1:816817/143‑1 (MQ=255)
                        attCTCTCCGCTTGGATGATGCTGCGCCATATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAGCGGTCTCGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGaa                                                                                                                 <  2:795283/143‑1 (MQ=18)
                            tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGCTGGCGCTACACTGCTGAACTGt                                                                                                             >  1:410930/1‑143 (MQ=255)
                            tctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGt                                                                                                             >  1:250175/1‑143 (MQ=255)
                                 gCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCaga                                                                                                        <  2:388074/143‑1 (MQ=255)
                                  cTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAAGGTTCagag                                                                                                       >  2:230854/1‑143 (MQ=255)
                                     agaTGATGCTGCGGCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt                                                                                                    <  1:554736/143‑1 (MQ=255)
                                         gatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTg                                                                                                >  1:229318/1‑143 (MQ=255)
                                              tgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg                                                                                           >  1:810387/1‑143 (MQ=255)
                                                cgcCACACGGTGTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCGGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat                                                                                         <  2:363108/143‑1 (MQ=18)
                                                 gcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGCAATGTTCAGAGTTTGGTGACGCGatc                                                                                        >  1:239585/1‑143 (MQ=255)
                                                  ccACATGCGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatca                                                                                       <  1:468032/143‑1 (MQ=255)
                                                   cataTGGATTGGACCGAAGCGGCTGACTTAATTGGTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGatcat                                                                                      <  1:143918/140‑1 (MQ=255)
                                                       tGGGTTGGACCGAAGCGGCTGACTTAATTGTTCAATGTATGGAAGGCGCAAGCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCGAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaa                                                                                  <  2:265157/143‑1 (MQ=18)
                                                        gggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCggag                                                                                 >  1:132564/1‑140 (MQ=255)
                                                            tGGACCGAAGCGGCAGACTTAATTGTTGGAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACAt                                                                             <  1:164054/143‑1 (MQ=255)
                                                                gcGAAGCAGCTAGCTTAATGGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGGATTCTAGTGTGTGATGGATGGCGCGAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTaa                                                                         <  1:522852/142‑1 (MQ=17)
                                                                 cGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTCAACTGCTGCACTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAAt                                                                        >  2:199923/1‑143 (MQ=255)
                                                                 cGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAGTGTTCAGAGTTTGGTGACGCGATGATCGCAAATATGTatt                                                                        >  2:637781/1‑141 (MQ=18)
                                                                 cGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAAt                                                                        <  1:848329/143‑1 (MQ=255)
                                                                 cGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGAAGGCGCTACCCCGCAGCATGGGTCAGAGGGTGGTGACGCGATCATCGAACCCATGTact                                                                        >  2:402751/1‑141 (MQ=17)
                                                                   aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGc                                                                      >  1:445552/1‑143 (MQ=255)
                                                                        gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACGTGTAATGCCGTAg                                                                 >  1:85108/1‑143 (MQ=255)
                                                                            aCTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGCTGGATGGCGCTAAACTGCTGAACTGTTGTGTGCCAAGTCACGCGAAAGTCGTAGACGTGTAATGCCGTAGTTTg                                                             >  1:205145/1‑143 (MQ=18)
                                                                              ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGACACGTTCCGAGTTTGGTGACGCGATCATCGAAAACGTGTAATGCCGTAGGGTGtt                                                           >  1:249893/1‑143 (MQ=18)
                                                                              ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGCAAACATGTAATGCCGTAGTTGGtt                                                           >  2:338720/1‑143 (MQ=255)
                                                                              ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGtt                                                           >  1:677727/1‑143 (MQ=255)
                                                                                 aTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGACATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTCATGCCGTAGGTTGCTaca                                                        >  2:368066/1‑141 (MQ=18)
                                                                                   tGTTACAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt                                                      <  1:354782/143‑1 (MQ=255)
                                                                                   tGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCCAAAACATGTAATGCCGTAGTTTGTTAAAtt                                                      >  1:861908/1‑143 (MQ=255)
                                                                                     ttAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATtta                                                    >  1:378966/1‑143 (MQ=255)
                                                                                      tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttat                                                   <  2:250175/143‑1 (MQ=255)
                                                                                       aaaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatt                                                  >  2:460435/1‑143 (MQ=255)
                                                                                            tATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACgg                                             >  1:127571/1‑143 (MQ=255)
                                                                                                gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGCAATGTTCAGAGTTTGGTGACGCGATAATCGCAAACATGTAATGCCGTAGTTGGTTAAATTTATTAACGGGAGc                                         >  2:44427/1‑143 (MQ=255)
                                                                                                gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTACCGGGGGc                                         >  1:162621/1‑143 (MQ=255)
                                                                                                      gcAATCAACGCGAAAACCGTAACCTATGACATCGAGTGTCTGATAGATGGCGCTAAACTGCTGAAATATTCAGAGGTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTGGTTACAATTATGAACGGGAGACTAACg                                   >  2:342264/1‑143 (MQ=21)
                                                                                                        aaTCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGGTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTCGATTTATTAACGGGAGCGCAACGCt                                 >  2:836103/1‑143 (MQ=25)
                                                                                                          tCAACGCGAGAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGTGATCGGAACGCTcc                               >  1:809442/1‑143 (MQ=25)
                                                                                                            aaCGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAACCATGTAATGCCGTAGTTTGTTAAATTTATTAACGGTAGCGTGACGCTCCCg                             >  1:506463/1‑143 (MQ=255)
                                                                                                                cgAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgttgt                         <  1:509767/143‑1 (MQ=255)
                                                                                                                     aCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGtttttt                    <  2:425752/143‑1 (MQ=255)
                                                                                                                     aCCGTAACCTATGACTGCGGGTGGCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGtttttt                    <  2:436594/143‑1 (MQ=255)
                                                                                                                      ccGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGACATGTTCAGAGTTTGGTGCCGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAAAGGGAGCGTAACGCTCCGCTTGTTTTTTg                   >  1:762650/1‑143 (MQ=25)
                                                                                                                           aCCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAgg              <  1:484716/143‑1 (MQ=255)
                                                                                                                              tATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCGGTAGTTTGTTAAATTCCTCAAAGGTAGCGTCACGCTCCCGTTGTTTTTTGTTAGgctg           >  2:189637/1‑143 (MQ=21)
                                                                                                                              tATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCGCGTTGTTTTTTGTTAGgctg           >  1:550751/1‑143 (MQ=255)
                                                                                                                              tATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGgctg           >  2:297090/1‑143 (MQ=255)
                                                                                                                                    ttCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACg     >  1:727369/1‑143 (MQ=255)
                                                                                                                                       gAGTGTCTGATGGATGGCGCTAAACTGCGGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTCACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGtt  <  1:716979/143‑1 (MQ=255)
                                                                                                                                       gAGGGTCGGATGCAGGGCGCTAAACCGCTGAAATGTTCAGAGTGTGGTTACGCGTTCATCGAGCTCCTGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGtt  <  2:684921/143‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTT  >  NC_000913/1196167‑1196444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: