Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,854,898 A→G 19.4% E326G (GAG→GGG)  emrD → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,854,8980AG19.4% 88.6 / 2.2 51E326G (GAG→GGG) emrDmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (19/19);  new base G (5/7);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGTCATC  >  NC_000913/3854664‑3855132
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             
tGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCg                                                                                                                                                                                                                                           >  1:1121496/1‑239 (MQ=255)
     aTTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGTTGCCGGGATGCAGTTTCCGCTCGCGACCCGCGGCCGGATGGGGCCGTTcc                                                                                                                                                                                                                                       >  1:900745/1‑238 (MQ=255)
     aTTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCTGGGATGCTGTTTCCGCTGGCGACCCGCGTCCCGATGGAGCGGTTcc                                                                                                                                                                                                                                       >  1:1188621/1‑238 (MQ=255)
     aTTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGGGCCGGGATGCTGTTTCCCCTGGCGACCCACGGCCCGTTGGGGCCCTTcc                                                                                                                                                                                                                                       >  1:1321405/1‑238 (MQ=255)
     aTTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGGGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGGGCCGGGAGGCTGTTTCCGCTGGCGCCCAGCGGCGCGATGGAGcccgtc                                                                                                                                                                                                                                        >  1:400435/1‑233 (MQ=255)
                     cAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGGGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGGGCCGTTCCCCTTCCTGGCGGGCACg                                                                                                                                                                                                                     >  1:740344/1‑239 (MQ=255)
                          ttttttGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGGGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGCCCGCCGGCGCGATGGGGCCGTTCCCCTTCCTGGCGGGGCGGGCTgg                                                                                                                                                                                                                >  1:1299640/1‑239 (MQ=255)
                                    cATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGGCTGGACGCTGCTCGTTCCCGCCCCGCTGTTCTTTTTCGGTGCCGGGGTGCTGTTTGCGCGGGCGCCCACGCGCGGCATGGGTGCGTTCCCCTTCCTGGCGGGGCCCGCTggggggcggggg                                                                                                                                                                                                      >  2:1021677/1‑229 (MQ=255)
                                      tGGTTTGCCGGACGCCCCAATAAACGGTTCTCCAGGTTAATGGGGCAGTCGGTTATCTGCGGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGAAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTcgg                                                                                                                                                                                                    <  1:816321/239‑1 (MQ=255)
                                                 aCGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACgg                                                                                                                                                                                                                    >  1:1220650/1‑212 (MQ=255)
                                                   gTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCCGGTCGGCGGGGTGCAAAAc                                                                                                                                                                                       >  1:275838/1‑239 (MQ=255)
                                                   gTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAACGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCAAGGCTGGGCCCCTGGCCGGCGGGCTGCcaaa                                                                                                                                                                                        >  1:451051/1‑238 (MQ=255)
                                                   gTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGGTTGGCGTGATGGATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCCCTTGCCACCAGCGGGCCGATGGAGCCGTTCCCCTTCCTTGCCGGGCCCGGGGGGCGCCCGGGTGGGGGGCGCGCaaaa                                                                                                                                                                                        >  2:785316/1‑239 (MQ=255)
                                                             aCGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGCCGGGGACGGCTGGCGCGCGGGTCGGGGGGCTGCAAAACATTGGTTCCg                                                                                                                                                                             >  1:923726/1‑239 (MQ=255)
                                                                           tAAGGTGGCAGTGGGTTAACGGCTGCCTGCTGGCTGGCTTGCTGATGTGGACCCCCGACTGGTTTGGCGTGATGAATTTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCg                                                                                                                                                              <  1:982795/239‑1 (MQ=255)
                                                                              tgtgGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGGCTGCAAAACATTGGTTCCGGCGTGCTGGCGTGGc                                                                                                                                                             >  1:1181745/1‑237 (MQ=255)
                                                                                                gctgcCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTTGCGGGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTCCGATGTTGCCGcca                                                                                                                                        >  1:578473/1‑237 (MQ=255)
                                                                                                gctgcCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGGGGTCTGCCACACCTTGGTTCCGGCGTGCTGGGCTCGCTTTCTCCGATGTTGCCCCa                                                                                                                                         >  1:956633/1‑238 (MQ=255)
                                                                                                gctgcCTGCGGGCTGGCTTGCTGATGTGGATCCCCGTCTGGGTTGCGGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCTCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCaa                                                                                                                                        <  1:111736/239‑1 (MQ=255)
                                                                                                 ctgcctgcGGGCTGGCTTGCTGATGGGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCaaa                                                                                                                                       <  1:108532/239‑1 (MQ=255)
                                                                                                    cctgcTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGGCGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGCCCCGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGGCCGGCCGGGCCCCTGGGCGGCGGGCTGCAAAAACTTGGTGCGCGCGGGGTGGCGGTCGTCTCCGGGATGTGGCGGCCAAccc                                                                                                                                    >  2:1022316/1‑238 (MQ=255)
                                                                                                            ctggctTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAggg                                                                                                                             <  1:680663/238‑1 (MQ=255)
                                                                                                              ggctTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGCCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGAGGTTGCCGCAAACCGGTCAGGGCa                                                                                                                           >  1:832369/1‑238 (MQ=255)
                                                                                                                ctTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGAGCTGGCGTCCCTCTCTCCGAGTGTTCCCCAAACCGGTCAGGGCAccc                                                                                                                        >  1:952144/1‑239 (MQ=255)
                                                                                                                              tccccggtgggTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTtgat                                                                                                           <  1:955883/229‑1 (MQ=255)
                                                                                                                                 ccGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGCTGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGCCGGGCGCGCGGGCCGGGGGGCTGCAAACAATTGGTTCCGGGGTGCTGGCGCCCCCCTCTGCGATGTTTCGCCAAACCGGCCCGGGCAGCCCGGGGgggttggttg                                                                                                         >  2:1028284/1‑233 (MQ=255)
                                                                                                                                      tGGTTTGGCGTGATGAATGTCTGGACGCTCCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTaa                                                                                                   <  1:224468/238‑1 (MQ=255)
                                                                                                                                       ggTTTGGGGTGATGAAGGTCTGGACGCTGCTCGTTCCCGCCGCGCGTTTCTTTTTCGGTGCTGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTTGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATg                                                                                                 <  1:986035/239‑1 (MQ=255)
                                                                                                                                                   tgaATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAggg                                                                                                                             <  1:542400/199‑1 (MQ=255)
                                                                                                                                                      aTGCCTGGTGGCTGCTCCTTCCCGGCGCGCTGTTCTTGTTCGGGGCCGGGATGGTGTTCCCGCGGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCgtg                                                                                  <  1:447904/239‑1 (MQ=255)
                                                                                                                                                                       gTTCCCCCCGCGCTGTTCTTTTTGGGTGCCGGGATGCTGTTTCCGCGGGCGGCCGGCGGCGCGATGGGGCCGTTCCCCTTCCTGGGGGGCACGGCTGGCGGGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCt                                                                 <  2:646504/239‑1 (MQ=255)
                                                                                                                                                                              ccgcGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGGTTGTTGATCGTGCTTTGCTGGCGTCCCCTGGCGAc                                                           >  1:1136853/1‑238 (MQ=255)
                                                                                                                                                                              ccgcGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGAcg                                                          <  1:1028284/239‑1 (MQ=255)
                                                                                                                                                                                   cTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCCAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGGCGCGGAt                                                     >  1:1089393/1‑239 (MQ=255)
                                                                                                                                                                                     gTTCTTTTTCGGGGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATg                                                    <  1:287504/238‑1 (MQ=255)
                                                                                                                                                                                      ttCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCGGGCTGCCGCTGGCGACGCGGATGTc                                                  >  1:1194315/1‑239 (MQ=255)
                                                                                                                                                                                      ttCTTTTTCGGTGCCCGGGTGGTTTGTGCGCGGGGGACCGGGGGGGGGGTGGGGGCGTTCCCCTTCCGGGGGGGCCCGGTTGGCGCGCTGGGTGGGGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTc                                                  <  2:1136853/239‑1 (MQ=255)
                                                                                                                                                                                          tttctggTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCAt                                              <  1:505079/234‑1 (MQ=255)
                                                                                                                                                                                              cGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCCCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGGGCTGGCTGCCGCTGGCGACGCGGGTGGCGCTGCAgg                                          >  1:550033/1‑239 (MQ=255)
                                                                                                                                                                                                   ccGGGATGGTGTTTCCGCTGGCGGCCAGCGGCGCGAGGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCa                                      <  1:1272561/238‑1 (MQ=255)
                                                                                                                                                                                                         gtctttttCCGCGGGCGGCCGTGGGCGGGCTGGGGCGGTTCCCCTGCCGGGCGGGCACGGTTGGCGCGGTTGTGCGCGGTCTTAAAACCGTGGGTTCCGGCGGGGTGGCGGCGCTCGCGTCGATTTTGCCGCAAACCGGTCAGGGCAGCCTGTGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTTGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCCGCCCGt                                <  2:1311042/233‑1 (MQ=255)
                                                                                                                                                                                                          ggtGTTTCCGCTGGCGACCAGGGGCGCGATGGGGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGttt                              <  1:301806/237‑1 (MQ=255)
                                                                                                                                                                                                               tcccGCGGGCGGCCGGGGGCGCGAGGGCGCCGTTCCCCTTCCTGGCGGGAACGGCTGGCGCGTTGGTCGGAGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAgcg                         <  1:1022316/237‑1 (MQ=255)
                                                                                                                                                                                                                 ccGCGGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCa                       <  1:948545/239‑1 (MQ=255)
                                                                                                                                                                                                                         gACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCg               <  1:1107582/239‑1 (MQ=255)
                                                                                                                                                                                                                              gcggcgCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCCGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCgcagca          <  1:1114245/239‑1 (MQ=255)
                                                                                                                                                                                                                                     gATGGAGCCGTTCCCCTCCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATc                                             <  1:562174/197‑1 (MQ=255)
                                                                                                                                                                                                                                     cttGGGGCCTTTCCCCTTCTTGGGGGGCACGGGTGGCGCGCTGGTCGGCGGGCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCTTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGtcat   <  2:923726/237‑1 (MQ=255)
                                                                                                                                                                                                                                       tGGGGCCGTTCCCCTTCCTGGGGGGACCGGCTGGCGGGCTGGTCGGGGGTCGGCAAAACATTGGTTCCGGCGGGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGtcatc  <  1:1243312/238‑1 (MQ=255)
                                                                                                                                                                                                                                       tGGGGCCGTTCCCCCTCCTGGCGGGCACGGCTGGCCGGCTGGTCGGTGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGtcatc  <  1:612017/238‑1 (MQ=255)
                                                                                                                                                                                                                                       tGGAGCCGTTCCCCGTCCTGGCGGGCCCGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGtcatc  <  1:385669/238‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             
TGTTTATTCTGCCGATTCCGGCAGCGTTTTTTGGCGCATGGTTTGCCGGACGTCCCAATAAACGCTTCTCCACGTTAATGTGGCAGTCGGTTATCTGCTGCCTGCTGGCTGGCTTGCTGATGTGGATCCCCGACTGGTTTGGCGTGATGAATGTCTGGACGCTGCTCGTTCCCGCCGCGCTGTTCTTTTTCGGTGCCGGGATGCTGTTTCCGCTGGCGACCAGCGGCGCGATGGAGCCGTTCCCCTTCCTGGCGGGCACGGCTGGCGCGCTGGTCGGCGGTCTGCAAAACATTGGTTCCGGCGTGCTGGCGTCGCTCTCTGCGATGTTGCCGCAAACCGGTCAGGGCAGCCTGGGGTTGTTGATGACCTTAATGGGATTGTTGATCGTGCTGTGCTGGCTGCCGCTGGCGACGCGGATGTCGCATCAGGGGCAGCCCGTTTAAGCGCACGTCACCGCAGCATCGTCATC  >  NC_000913/3854664‑3855132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: