Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,103,633 A→C 15.1% V448G (GTG→GGG)  cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,103,6330AC15.1% 301.3 / 3.2 126V448G (GTG→GGG) cpxAsensory histidine kinase in two‑component regulatory system with CpxR
Reads supporting (aligned to +/- strand):  ref base A (59/40);  new base C (2/25);  total (61/65)
Fisher's exact test for biased strand distribution p-value = 6.20e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCC  >  NC_000913/4103396‑4103863
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                         
tctcCGCGTCCGGGTTAGCGAAAGGCTGGACGCGGCAGATGCAAAAGCGCC‑GAGTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCACATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCGGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCAc                                                                                                                                                                                                                                          <  1:9241/239‑1 (MQ=255)
ccccggtggCCGGGTTACCGAAACGCGGGGCGCGGACGATGCAAAAGCGCC‑GACGGAGCGGCAAGATCGAAGATTTTTCC‑CGGCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCAc                                                                                                                                                                                                                                          <  1:244475/231‑1 (MQ=39)
   ccGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCC‑GACTGCGCGGCAAGATCGACGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGGGTAGGCCTGCTAAGACGCTATCAGCGGCGCCTCAGGCATCCTGCTC‑AAAGGCCGC‑TTGCGGCGCAAAGGCCTACTCCTGCCTGCAAATGCGAAGTTTAACTCCCCTTAAGAC‑GCGGCAACAAAACAACCa                                                                                                                                                                                                                                        >  2:158531/1‑238 (MQ=255)
            ggTTATCAACAGGCGGGCCGCGACTGATGCAAAA‑CGCCAGACTGGACGGCAGGTACAAGGTTTTTCCC‑CGCCGGTTGTGGGAAAAAAACCCCCCGGAGTGTGGGCCTGAAAAGCCGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑cc                                                                                                                                                                                                                             <  2:181833/239‑1 (MQ=255)
                 tCGAAAAGCTGGCCGCGGACGATGCAAAAGGGCC‑GACTGGGCGGCAAGATCGAAGATTTTCCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCa                                                                                                                                                                                                                        <  1:1217170/239‑1 (MQ=255)
                      aggCTGGACGCGGAAGATGCAAAAGCGCC‑GACTGGAGGGCAAGATCGAAGGTTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCggg                                                                                                                                                                                                                   <  1:279693/237‑1 (MQ=255)
                      aaGCTGGACGCGGACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATCCA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCgg                                                                                                                                                                                                                    >  1:1247397/1‑238 (MQ=255)
                      aaGCTGGACGCGGACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCggg                                                                                                                                                                                                                   <  1:177512/239‑1 (MQ=255)
                       aGCGGGCCGGGGACGATGAAAAGGCGCC‑AACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTGGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGc                                                                                                                                                                                                                  <  1:1291220/239‑1 (MQ=255)
                            gACGCGGACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGCCGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AACTGCCGA‑ATGCGACGTAAAGGCCTAATCCTACCTGCAAATGCGAAGCTTCAATCCCCTTATACA‑GCGGAAACCAAATCCACAACCGAAAA‑CAGCCCACCggggcggct                                                                                                                                                                                                             >  2:170707/1‑233 (MQ=255)
                              cgtgtacaagtcAAAAGCGCA‑GACTGGGCGGCAAGTTCGAAGATTTTACC‑CGCCGGTGTTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGtcttc                                                                                                                                                                                                           <  1:325569/228‑1 (MQ=255)
                               cgggacagtcaaaaaGCGCC‑GACTGAGCGGCAAGAGCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCGGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTAGCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGtcttc                                                                                                                                                                                                           <  1:974664/227‑1 (MQ=255)
                                  gACGATGCAAAAGCGCC‑GACTGAGCGGCCAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGCGTGTAGGACTGATAAGACGCTATCCGCGTCGCATCATGCATCCTGCTC‑AAATGCCGG‑GTCCGGAGTAAACCCCTTATCCTGCCTTCAAATGCGAAGTTTAACTCCGCTTAAACA‑GCGGACACCAAACAACCAGCCGTAAC‑CCGACCAGCGAGCTGccttcgc                                                                                                                                                                                                         >  2:806725/1‑235 (MQ=255)
                                  gACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTg                                                                                                                                                                                                         >  1:982191/1‑237 (MQ=255)
                                         cAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGTTTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCAcc                                                                                                                                                                                                 <  1:1080680/238‑1 (MQ=255)
                                                gcC‑GACTGGGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGGGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCa                                                                                                                                                                                           <  1:1224731/237‑1 (MQ=255)
                                                 cc‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCACCGGGCTGCCTTCTGCCTTCACCCAGCCACGa                                                                                                                                                                                        >  1:1210225/1‑239 (MQ=255)
                                                 cc‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATACCCACCCGACAA‑CCGCCCACCGGGCTGTCCTCTGCCTGCCCCCACCCCCgc                                                                                                                                                                                        >  2:749910/1‑238 (MQ=39)
                                                 cc‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGAAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGCCTTCTGCCTTCACCCAGCAACGa                                                                                                                                                                                        >  1:565133/1‑239 (MQ=255)
                                                     aCTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGGCTTCTGCCTTCACCCAGCCACGAtgc                                                                                                                                                                                     >  1:750963/1‑239 (MQ=255)
                                                       tgggCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGAtgctg                                                                                                                                                                                   <  1:170938/236‑1 (MQ=255)
                                                         aGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGAtgcg                                                                                                                                                                                    >  1:1001428/1‑235 (MQ=255)
                                                         aGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCACCGGGCTGTCTTCTGCCTTCACCCAGCCACGATgctgct                                                                                                                                                                                 >  1:617383/1‑239 (MQ=255)
                                                         aGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCACCGGGCAGTCTTCCGCCTTCACCACCCCACAAtgccgcc                                                                                                                                                                                 >  2:79790/1‑238 (MQ=39)
                                                                       ggATTGTTCCGCGCGGGTGTTGGGGAAAAACCCCCCCGGGGGGGAGGCCTGATGAGACGCGATGTGCGTGTCCTCAGGGCGCCTGGTC‑AATTGCCGG‑TTGCGGCGGAAAAGCATTATCCGTCCAGCAAATGCGAAGTTTAACTCCGCTTATACA‑GGGGCAACCAAAACCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCTCCCAGCCACGATGCTGCTG‑AATGGCGGttt                                                                                                                                                                    <  2:898943/237‑1 (MQ=255)
                                                                             ttCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCCAATCACCAGCCGTAAA‑CCGCCCCGCGGGCTGTCTTCTGCCTTCACCCAGCCACCATGCTGCAG‑CAGGGCGGGTTccaaaaa                                                                                                                                                             >  2:741723/1‑232 (MQ=39)
                                                                                cccgagggTTGGGGGGAAAAGAACCCCGGGAGTGTAGGCCTGAAAAGACGCTAGCAGCGTCGAATCAGGCATCCTGCCC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGc                                                                                                                                                          <  1:158531/233‑1 (MQ=255)
                                                                                c‑cgccgGTTGTGGGGAAAATAACCCCCGGGGTTGAGGCCTGATAAGACTCTACCAGCGTCGCACCAGGGTACCTGCTA‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGcc                                                                                                                                                         <  2:1245370/239‑1 (MQ=255)
                                                                                 ccccctttttgggggAAAAAACCCCCCGGGTTTTTGGCCGGATAAGACCTTATCGGCGTCGTATCAGGCATGCTGCTA‑AAATGCCGG‑AGGCGGCGTAAACGCCTTACCCGGCCTGCAAATGCGAAGTTTACCTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGcc                                                                                                                                                         <  2:551185/228‑1 (MQ=255)
                                                                                    ccgGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGCCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGGGGGTTtaacaaatcccac                                                                                                                                                       >  1:1027918/1‑231 (MQ=255)
                                                                                    ccgGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGACAA‑CCCCCCAGCCGGCTGTCTTCTGCCTTCCCCCAGCCACGGTGCTGCTGAAAGGGGGGTTTcaccaacccccc                                                                                                                                                        >  2:863018/1‑232 (MQ=255)
                                                                                      gtttGGGGGGAAAATTACCCCCGGGTTTTAGGCCTGGAAAGAGGCTATCAGCGTGCCATCCGGCATCCTGCTC‑AAAGGCCGG‑ATGCGGCGTAAACGCCTTAACCTGCCGGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAACCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGccc                                                                                                                                                    <  2:544303/237‑1 (MQ=255)
                                                                                                  aaTAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCACATGCGAGGTTTAACTCCGCTTATACA‑GCGGCAACCCAATCACCAGCCGTAAA‑CCCCCCCACGGGCTGTCTTCTGCCTTCACCCAGCCACGCTGCTGCTG‑CAGGGCGGGTTCCACAAACCCCACCCCCAAAcccaccc                                                                                                                                         >  2:1320795/1‑233 (MQ=38)
                                                                                                  aaTAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAAACGCCAGCCCCCAACCTGTAc                                                                                                                                         >  1:822063/1‑238 (MQ=255)
                                                                                                     tccccccGGAGTTTGGGCCTGAAAGGGCCGTTTCCGCTCCGGATCGGGCGTCCTGCTC‑AAAGGCCGG‑ATGCGGCGAAAACCCCTTACCCTGCCTGCAAAGGCGAAGTTTAACCCCGCTTATACG‑GCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAACCCTGTACCGcc                                                                                                                                     <  2:104124/237‑1 (MQ=255)
                                                                                                     accccccGGGGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACCCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGcc                                                                                                                                     <  1:1209540/237‑1 (MQ=255)
                                                                                                     accccccGGAGTGTAGGCCTGAAAAGACGCTATCAGGGTCGAATAAGGCATCCTGCTC‑AAATGCCTG‑ATGCGGCGTAACCGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGcc                                                                                                                                     <  1:297131/237‑1 (MQ=255)
                                                                                                     aaCCCCCGGGGTGTAGGCCTGATAAGACGCTATCAGCGGCGCATCAGGCCTCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACCCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGcc                                                                                                                                     <  1:691898/239‑1 (MQ=255)
                                                                                                       cccccGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGGTTTAACAATACCCAGCCCCCAAcccttaaccccca                                                                                                                                   >  1:773109/1‑228 (MQ=39)
                                                                                                            ggAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATACCCAGCCCCAAACCTGTACCCCCCGAttt                                                                                                                               >  1:880692/1‑237 (MQ=255)
                                                                                                                tgtAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCATTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAACCCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAAAAATAGCCAGCCCCAAACCTGTACCGCCACATTCaagg                                                                                                                           >  1:953938/1‑236 (MQ=255)
                                                                                                                tgtAGGCCTGATAAGACGCTATAAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCCCGATGCTGCTG‑AATGGCGGGTTCCACAATCGCCCGCCCCCCACCCGTACcccccacttaacca                                                                                                                           >  1:1325732/1‑228 (MQ=255)
                                                                                                                     gcgtGATAAGCCGCTATCAGGGGCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTAGCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATGCACGATcgcg                                                                                                                      <  1:252232/235‑1 (MQ=255)
                                                                                                                          aTAAGGCGCTATCAGCCGCGCATCCGGCATCTTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTCTCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTc                                                                                                                <  1:1264142/239‑1 (MQ=255)
                                                                                                                          aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCCCGATGCTGCTG‑AATGGCGGTTTCAACAATCCCCACCCCCCAACCTGTACCCCCAGATTCACCATCGCCTGCtga                                                                                                                >  1:174115/1‑237 (MQ=255)
                                                                                                                          aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCCACAATCGCCAGCCCCAAACCTGTAACGCCAGATTCACGATACCGGGCTTc                                                                                                                >  1:3089/1‑239 (MQ=255)
                                                                                                                          aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCCAACCCGTACCGCCAGATTCACGATCGCGGGCtt                                                                                                                 >  1:292235/1‑238 (MQ=255)
                                                                                                                          aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCCGTAACGCCAGGTTCACGACcgccgtgttc                                                                                                                >  1:987566/1‑232 (MQ=255)
                                                                                                                          aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGGTTTAACAAATCCCAACCCCAAACCCTAAACGCCCAATTCACAGTCGCGGGCttt                                                                                                                >  1:517010/1‑238 (MQ=255)
                                                                                                                          aTAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGCCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCCAACCCGTACCGCCCAGTTCACGATAGCCGGCtt                                                                                                                 >  1:28178/1‑238 (MQ=255)
                                                                                                                            aaGACGCTATCAGCGTCGCATCAGGCATCCTGCTA‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCAt                                                                                                              <  1:79790/239‑1 (MQ=255)
                                                                                                                               gcGCTACCCGCGTCGAATCGGGCCCACTGCAC‑AAATGCCGT‑ATGCGGAGAAAACCCTTTATCCGCCCGGCAAATGGAAAGTTTACCGCCGCTTAGCCA‑GCGGCAACAAAACCCCCGGCCGTAAA‑CCGCCCAGCGGGCTGTTTTCTGCCTTCGCCCAGCAACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAATCTGTACCGCCAGATTCGCGATCGCGTGCTTCATCgg                                                                                                           <  2:583380/238‑1 (MQ=255)
                                                                                                                                cGCTATCAGGGTCCCTTCAGGCCTCC‑GCACTAAAGTCCGG‑AGGCGGCGTAAACGCCTTTTCCTGCCTGCAAAGGCGAAATTTAACCCCGCTTATACA‑GCGGCAACCAAATCCCCAGCCGTAAC‑CCGCCCAGGGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCg                                                                                                            <  2:292235/237‑1 (MQ=255)
                                                                                                                                 gCTAACAGCGGCGCATCAGGCATCCTGCTC‑AAAGGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCgg                                                                                                           <  1:823068/237‑1 (MQ=255)
                                                                                                                                   tATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGcc                                                                                                                                                         <  1:1144970/189‑1 (MQ=255)
                                                                                                                                   tATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCCCGCACTTCCACCGTACg                                                                                                       >  1:274644/1‑239 (MQ=255)
                                                                                                                                    aTCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCCGTACCGCCAGATTCACGATCCCGGGCTTCAACGGTACGa                                                                                                      >  1:767384/1‑239 (MQ=255)
                                                                                                                                    aTCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCACAACCCGTACCGCCAGGATCCCGATCCCGTGCTTCATCGGTACGa                                                                                                      >  1:41893/1‑239 (MQ=255)
                                                                                                                                    aTCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGATTCAACCATCGCCAGCCCCAAACCCGTACCCCCAGATTCACCATCGCCTGCTTCCTCGGGAccg                                                                                                      >  1:481740/1‑237 (MQ=255)
                                                                                                                                          gTCGCAGCAGGCACCCTGCTC‑AAATGCCGG‑GTGCGGCGGAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGa                                                                                                  <  1:291755/237‑1 (MQ=255)
                                                                                                                                             gCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAGCTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGc                                                                                                                                                                                                                      >  2:489395/1‑119 (MQ=255)
                                                                                                                                             gCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGc                                                                                                                                                                                                                      <  1:489395/119‑1 (MQ=255)
                                                                                                                                             cccccgggCATCCTGCCC‑AAA‑GCCGGTGTGGGGCCTAAACGCCTTAGCCTGCTGGCAAAGCCGAATTTTATCTCGGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTTAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAacgga                                                                                             <  2:481740/233‑1 (MQ=255)
                                                                                                                                                       tCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATc                                                                                    <  1:384003/238‑1 (MQ=255)
                                                                                                                                                         ccgttC‑AAATCCGGG‑TTGGGGGGAAACCCCCTTACCCCGCCCGCAAATGGCAAGTTTAACCCCGCTTTTCAC‑GCGGCAACCAAATCACCAGCCGGAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGt                                                                                 <  2:1001428/235‑1 (MQ=255)
                                                                                                                                                            cTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGGAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTTAACAATCGCCAGCCCCCAACCCGTACCGCCAGATTCACGATCCCGTGCCTTCTCGGTCAGATAAAAAGGCAGGAAAAACTGTTc                                                                               >  1:1288017/1‑238 (MQ=255)
                                                                                                                                                             tC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCtttttcc                                                                             >  1:31607/1‑236 (MQ=255)
                                                                                                                                                                 aaTGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCCAACCTGTACCGCCAGGTTCCCGATCGCGTGCTTCATCGGTACGATAGAACCGACGGAAAAACTGTTcccgg                                                                           >  1:1142808/1‑237 (MQ=255)
                                                                                                                                                                     ccGG‑TTCCGGCGTTACCTCCTTACCCTGCGCGCAAATGGAAAGTTTAACCCCTCTTA‑ACACGGGGCAACCCAACCCCCAGCCGTAAA‑CCCCCCAGGGGGCTGTCTTCTGCCTTCACCCAGCCCCGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTc                                                                      <  2:1114828/239‑1 (MQ=255)
                                                                                                                                                                            gcggcgTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCt                                                                <  1:1320298/239‑1 (MQ=255)
                                                                                                                                                                             cggcgTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCAc                                                                                                                                                                                                                                          <  2:31108/70‑1 (MQ=255)
                                                                                                                                                                             cggcgTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCAc                                                                                                                                                                                                                                          >  1:31108/1‑70 (MQ=255)
                                                                                                                                                                              gggcaaaaCCCCTTACCCTGCCTGAAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑a                                                              <  1:479308/233‑1 (MQ=255)
                                                                                                                                                                                   aaaCGCCTTATCCTGGCTGAAAATGCGAAATTTAACTCCGCTTATACA‑GCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGcc                                                         <  1:863018/238‑1 (MQ=255)
                                                                                                                                                                                       gCCTTCTCCTGCCTGCAAAGGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGAc                                                    <  1:43901/239‑1 (MQ=255)
                                                                                                                                                                                          ttATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑CATGGCGGGTTTAACAAACCCCCGCCCCCAACCCCAGACCCCCCAGTCACCACAGCGGGCTTCACCGCTACCATAAAACAGACCGAAAAAATGTTCCCGCACTTCCGGGCTCAACGCCAgaaaa                                                   >  2:647476/1‑234 (MQ=255)
                                                                                                                                                                                            aTCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCAt                                                                                                              >  1:25359/1‑177 (MQ=255)
                                                                                                                                                                                            aTCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATAGCCAGCCCCAAACCCGTACCCCCAGATTCACGATCGCGTGCTTCATCGGTACGATAAAAAGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCaacc                                               >  1:1245978/1‑236 (MQ=255)
                                                                                                                                                                                            aTCCCGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCAt                                                                                                              <  2:25359/177‑1 (MQ=255)
                                                                                                                                                                                                 gcctgcAAATGGGAATTTTACCCCCGCTTATACA‑GCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCgtcgtc                                          <  1:146487/239‑1 (MQ=255)
                                                                                                                                                                                                 ccctcaaaaCGCGAATTTTCCCCCCGTTTATAGC‑GCGGCAACCAAACCCCCCGCCGTAAA‑CCCCCCAGGGGGTTTTTGTCGCCCTTCCCCCAGCCACGATGCTGCGG‑AATGGCGGTTTCAACAAGCGCGAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCgtcgtc                                          <  2:1247397/233‑1 (MQ=255)
                                                                                                                                                                                                  cctgcAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCAc                                                                                                                                                                                                                                          >  1:337883/1‑49 (MQ=255)
                                                                                                                                                                                                   ctgcAAATTCGAAGTTTAACCCCGCTTAAACA‑GCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCa                                        <  1:586220/239‑1 (MQ=255)
                                                                                                                                                                                                   ctgcAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTTCTTCATCGGGACGATAGAACGGACGGAAAAACTGTTCGCGAGCTTCCCGGCT‑AAAGCCAGGACCATCGGCGTcc                                         >  1:1324877/1‑238 (MQ=255)
                                                                                                                                                                                                   cgccAAATGCGAAGTTTACCTCCGCTGATCCA‑GCGCCAACCAAACCCCCAGCCTTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTGCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAAGCGCCACCCCCAAACCTGGACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCa                                        <  2:3089/236‑1 (MQ=255)
                                                                                                                                                                                                   ccccAATTGCGAAGTTTCACCCCCCTTTCACG‑GCGCGAACCAAACCCCCAGCCGTAAC‑CCGCCCAGGGGGCTTTCTTCCGCCTTCACCCCGCCCCGATGCCGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCa                                        <  2:982191/236‑1 (MQ=255)
                                                                                                                                                                                                       aaaTGCGAATTTTAACCCCGCTTATACA‑GCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGt                                    <  1:631456/239‑1 (MQ=255)
                                                                                                                                                                                                         aTGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTaa                                  <  1:542126/239‑1 (MQ=255)
                                                                                                                                                                                                                 tttACCCCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATa                          <  1:1028088/239‑1 (MQ=255)
                                                                                                                                                                                                                  ttAACTCCGGTTATACA‑GGGGCAACCAAACCCCCAGCCGTAAA‑CCGCCCAGCGGGCTTTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATa                          <  1:476902/238‑1 (MQ=255)
                                                                                                                                                                                                                           cTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCCAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCTGCGTTCACCGTCAGGGGGATAACGCCttttt                >  1:833983/1‑237 (MQ=255)
                                                                                                                                                                                                                           cTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGGTAGAACGGACGGAAAATCTGTTCGCGAACTTCCCGGCT‑AACGCCAGGACCATCGTCGTCCACCCGAATTGTGGTAccagccttt                 >  1:1030462/1‑231 (MQ=255)
                                                                                                                                                                                                                              taacacgCGGCAACCAAATCCCCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCGGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCt              <  1:13165/233‑1 (MQ=255)
                                                                                                                                                                                                                                    cgcgcgcccAAATCCCCCGCCGTAAA‑CCCCCGCGGGGGTTGTCTTCCCTCTCCCCCCGCCCCCTGTCCTGCTG‑AATGGCGGTTTCAACAACCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCa        <  2:1008828/232‑1 (MQ=255)
                                                                                                                                                                                                                                    ccgctaaCAAAACCCCCACCCGCAAC‑CCGCCCAGGGGGTTGTCTCCTGCCTCCACCCAGCCCCGCTGCTGCGG‑AATGGCGGTTTCAACAATCGCCAGCCCAAAACCTGTCCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCa        <  2:308723/234‑1 (MQ=255)
                                                                                                                                                                                                                                    ccccccaCCAAACCCCCCGCCCTAAC‑CCCCCCTGGGGGCTGTCTTCCGCCTTCCCCCAGCCACGATGCCGGTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTCCCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTGCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCa        <  2:446590/233‑1 (MQ=255)
                                                                                                                                                                                                                                     cGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCCTCGTCGTCCCCCGTAATGGTGATACCGTCTTTTTCTACCGCaa       >  1:38435/1‑239 (MQ=255)
                                                                                                                                                                                                                                     cGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAAACTGTTCGCGATCTTCCCGGCT‑AACCCCCGGACCATCGTCGTCCACCCTAATGGGGATACCGTCTTTATCTACCGcca       >  1:87726/1‑237 (MQ=255)
                                                                                                                                                                                                                                     cGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCCTGCTTCATCGGTACGATAGAACGGACGGAAAAACTGTTCGCGATCTTCCCGGCT‑AACGCCAGGCCCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCaa       >  1:1073948/1‑239 (MQ=255)
                                                                                                                                                                                                                                          aCCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCCCGGTCTTCCGGGCT‑AACCCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTTTCTACCCCAAAGccc  >  1:308723/1‑239 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                         
TCTCCGCTGCCGGGTTATCGAAAAGCTGGACGCGGACGATGCAAAAGCGCC‑GACTGAGCGGCAAGATCGAAGATTTTTCC‑CGCCGGTTGTGGGGAAAATAACCCCCGGAGTGTAGGCCTGATAAGACGCTATCAGCGTCGCATCAGGCATCCTGCTC‑AAATGCCGG‑ATGCGGCGTAAACGCCTTATCCTGCCTGCAAATGCGAAGTTTAACTCCGCTTATACA‑GCGGCAACCAAATCACCAGCCGTAAA‑CCGCCCAGCGGGCTGTCTTCTGCCTTCACCCAGCCACGATGCTGCTG‑AATGGCGGTTTCAACAATCGCCAGCCCCAAACCTGTACCGCCAGATTCACGATCGCGTGCTTCATCGGTACGATAGAACGGACGGAAAATCTGTTCGCGATCTTCCGGGCT‑AACGCCAGGACCATCGTCGTCCACCGTAATGGTGATACCGTCTTTATCTACCGCAAAGCCC  >  NC_000913/4103396‑4103863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑