Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,201,278 A→C 20.6% G137G (GGT→GGG zraP ← Zn‑dependent periplasmic chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,201,2780AC20.6% 140.0 / 3.7 59G137G (GGT→GGGzraPZn‑dependent periplasmic chaperone
Reads supporting (aligned to +/- strand):  ref base A (41/4);  new base C (2/12);  total (43/16)
Fisher's exact test for biased strand distribution p-value = 1.25e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGCATGCGATGGAACAGACCGTGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTT  >  NC_000913/4201047‑4201515
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             
gcTGGCATGCGATGGAACAGACCGTGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCCTGGCTGGAACCGCCCGAAAGTTCGCAATTACTGTTAGTGGCAAACCCTGTTTTCTGTCGCTGGCAACCAAACGGACAAACGGCTTGATTTCCACGTGGcccacccccataa                                                                                                                                                                                                                                        >  2:1312075/1‑238 (MQ=255)
gcTGGCATGCGATGGAACAGACCGTGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAATGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGccacaacccatag                                                                                                                                                                                                                                        >  1:892099/1‑238 (MQ=255)
 cTGGCATGCGATGGAACAGACCGTGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGcccataccccgtt                                                                                                                                                                                                                                        >  1:203410/1‑234 (MQ=255)
 cTGGCATGCGATGGAACAGACCGTGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATg                                                                                                                                                                                                                                       >  1:58146/1‑239 (MQ=255)
  tGGCATGCGATGGAACAGGCCGTGACCACCTGGGAAGGGCAACCCATTAAACCTGATTTAGATTCGGAGGCGATAGCGCATTTCGAGCGCCGCCAGACCCGCTTTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATg                                                                                                                                                                                                                                       <  1:963658/238‑1 (MQ=255)
           gggtaaCAGTCCGGCACCCCCGGGGAGGGGAAACCCATCAACCCTGATTTAGATGCGCAGGCGTTAGGCAATTGCGGGCGCCCGCAGGGCCGCTCTTCTGTTGAGGTCAGCGGGGCATGGCGGGAAGCGCCCGAAGGTCCGAAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGccacc                                                                                                                                                                                                                              <  2:772623/234‑1 (MQ=255)
                    aCCGTGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAgcc                                                                                                                                                                                                                     >  1:633352/1‑238 (MQ=255)
                      cGTGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCCCCTCAgccgcc                                                                                                                                                                                                                  >  1:882541/1‑239 (MQ=255)
                      cGTGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTCATTTACCAGTGGCCAATACCCCTTTTACCGCccccactcacaccccc                                                                                                                                                                                                                  >  1:1141487/1‑229 (MQ=255)
                        tGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCACACTCTGATTTCTGTCGCTGGCAACCCACCGAGAAAACGTTCTGATTTACCACTTGCCACTACCCATATGACCCCCaccacccaccccccccc                                                                                                                                                                                                                >  2:292411/1‑228 (MQ=255)
                        tGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGt                                                                                                                                                                                                                >  1:229338/1‑239 (MQ=255)
                        tGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCACTGGCCCATACCCATATGACCGCCACAACCGCAACCGCCGt                                                                                                                                                                                                                >  1:967991/1‑239 (MQ=255)
                         gACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGGTTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGccgccgga                                                                                                                                                                                                               >  1:666237/1‑237 (MQ=255)
                         gACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTCTTGATTTTCCAGTGGCCCCTCCCCATATGACGCCCACCACCACGGCAACCGTa                                                                                                                                                                                                               >  1:194377/1‑239 (MQ=255)
                         gACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAAAGTTCTGATTTACCAGTGGCCCATACCCATAGGACCGCCACCACCGGACCCGCCGTa                                                                                                                                                                                                               >  1:987011/1‑239 (MQ=255)
                                   gAAGGGAAAACCATTAACCCTGATTTAGTTTCGCGGGCGATAGCGCATATCGAGCGCCGCAAGGGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCCCCCGAAGGTTCGAAATTACTGTTAGTGGAAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGGGGCCCATCCCCATATGACCGCCACCACCGCAGCCGCCGTAgcccatgcc                                                                                                                                                                                                      <  1:1008630/238‑1 (MQ=255)
                                         cAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGCCCGCCACCACCGCAGCCGCCGTAACCCATGCCCATTcc                                                                                                                                                                                                >  1:757656/1‑238 (MQ=255)
                                                 tAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACCCTGATTTCTGTCGCTGGCAACCCAACGAGCAAACGATCTGATTTACCAGTGGCCCATCCCCATATGACCCCCCCCACCGCCTCCGCCGCAACCCACTCCCATTCCCGCCCcccg                                                                                                                                                                                       >  2:1017887/1‑239 (MQ=255)
                                                        gATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAAt                                                                                                                                                                                 >  1:640485/1‑238 (MQ=255)
                                                         aTTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATa                                                                                                                                                                                >  1:561122/1‑238 (MQ=255)
                                                                   gCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATTCCCATTCCGGCTCCGCCCGGAATACCCGCCTCAg                                                                                                                                                                      >  1:254367/1‑238 (MQ=255)
                                                                          aTAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAACCGTTCTGATTTACCACTGGCCCCTACCCATATGACCGCCACCACCGCAGCCGCCGGAGCCCATACCCCTTCCGCCTCCCCACGGCAAAACCCCTTccccccccccg                                                                                                                                                              >  2:910278/1‑229 (MQ=255)
                                                                                   atCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCCCGCGGAATACCCGCTTCAGCCATCGCGATATCCCGt                                                                                                                                                     >  1:64311/1‑239 (MQ=255)
                                                                                       aGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCCGGCAACCCAACGAGAAACGGTTCTGATTTACCAGTGGCCCATACCCATATGAACGCACCCACCGCCGCCGCCGTAGCCCCTGCCCATTCCGGCTCCGCGCCGAATAACCCCCTCAACCCCCCCGGTAACCCGTTTc                                                                                                                                                  >  2:717691/1‑238 (MQ=255)
                                                                                        gcgcCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCa                                                                                                                                                 >  1:28360/1‑238 (MQ=255)
                                                                                         cgccgcCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATCCCGGCTCCGCGCGGGATACCCGCTTCAGCCATCGCGATATCTCGTTTCAc                                                                                                                                                >  1:664313/1‑238 (MQ=255)
                                                                                                                    gTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCCTTCCGGCTCCGCGCGGCATACCCCCTTCAGCCATCGCGATATCTCGTTTCACCCGTACACCATCTAACGACTGACGCa                                                                                                                      >  1:1148632/1‑237 (MQ=255)
                                                                                                                    gTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCaaa                                                                                                                    >  1:175000/1‑239 (MQ=255)
                                                                                                                                 ggCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCtt                                                                                                       >  1:461550/1‑239 (MQ=255)
                                                                                                                                   cTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAACCCATCCCCATTCCGGCTCCGCCCGGAATACCCCCCTCCGCCATCGCGAAATCTCGTTTCACCCCTAATCCATTTAACGCCTGCCCCAAATTCTCCACTCtttt                                                                                                     >  2:1043659/1‑237 (MQ=255)
                                                                                                                                   cTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTCACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGCATACCCCCTTCAGCCATCGCGATATCTCGTTTCACCCCTAACTCATCTACCGACTGTCGCACATTCTcccccccttt                                                                                                      >  1:331476/1‑230 (MQ=255)
                                                                                                                                            cccGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGCTTTCTGTCGCTGGCAACCCAACGAGACAACGTTCTGATTTCCCAGTGGCCCCTACCCATATGACCGCCAACACCCCAGCCGCCGTAGCCCATGCCCATTACGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCCCGTTTCACCCGTAACTCATCTAACGCCTGCCGCAAATTCTCCCTCTCTTTGGcacccg                                                                                              >  2:885165/1‑232 (MQ=255)
                                                                                                                                            cccGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACcccg                                                                                            >  1:1070802/1‑239 (MQ=255)
                                                                                                                                                    ttCGTAATTCCGGTTAGGGGCAACCTTTGATTTCTGGCGCTGGACACCCAACGAGAAAACGTTCTGATTTCCCAGTGGCCCATCCCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAAttt                                                                                     <  1:1104878/238‑1 (MQ=255)
                                                                                                                                                           ttgcTGTTAGTGGAAACCTCTGTTTTTGGTCGCTGCCACCCCAACGAAAAAACGTTCTGATTTACCAGGGGCCCATCCCCATATGCCCCCCCCCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTa                                                                             <  1:1312075/236‑1 (MQ=255)
                                                                                                                                                           cacaggTTAGTGGCAATCTCTGTTTCCGGTCGCTGGCAACCCAACGATAAACCGTTCTTATTTACCAGTGGCCCATCCCAATATGCCCGCCCCCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCAACCGCGTTAATTTtgctgc                                                                               <  1:622283/232‑1 (MQ=255)
                                                                                                                                                                gTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGTTGCTATCCgg                                                                        >  1:635480/1‑239 (MQ=255)
                                                                                                                                                                   aGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGGAGCCCAGGCCCATTCCGGCTCCGCGCGGAATACCCCCTTCCGCCAACGCGATATCCCGTTTCACCCGTAACTCATCTAACGTCTCACGCCCATGCCCCCTCTCTTTTGCGACCCAGTTAATTTtgttgcgaccgggggg                                                                     >  2:672223/1‑229 (MQ=255)
                                                                                                                                                                       gCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCCCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGtt                                                                  >  1:1158439/1‑238 (MQ=255)
                                                                                                                                                                        cAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTcg                                                                >  1:910224/1‑239 (MQ=255)
                                                                                                                                                                           aCTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCCCGGAATACCCGCCTCAGCCATCCCGATATCTCGTTTCACCCGTACCCCACCTAACGACTGCACCAAATTCTCCATCTCTTTGCCACACGCGTTAATTTTCCTCCTATCCgaggggctgtg                                                               >  2:622283/1‑227 (MQ=255)
                                                                                                                                                                               tgattcgttgcGCCGGCCACCCAACGAGAAAACGTTCTGATTCACCAGTGGCCCATCCCCATATGACCGCCACCCCCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTaa                                                         <  1:757472/229‑1 (MQ=255)
                                                                                                                                                                               tGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCTCGGCTaa                                                         >  1:361066/1‑239 (MQ=255)
                                                                                                                                                                               tGATTTCTGCCGCTGACAACCCAACGAAAAACCTTTCTTATTTCCCGGGGCCCAATCCCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTaa                                                         <  1:231515/239‑1 (MQ=255)
                                                                                                                                                                                 ttttGTGTCCCTGGAAACCCAAGGGAAAAACGTTCTGTTTTACCAGTGGCCCATCCCCATAGCACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACa                                                       <  1:1043659/238‑1 (MQ=255)
                                                                                                                                                                                 aTTTCGGTCGCGGGCACCCCAAGGAAAAACCTTTCTGATTTCCCAGGGGCCCATCCCCATATGACCGCCCCCCCCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACa                                                       <  1:150213/239‑1 (MQ=255)
                                                                                                                                                                                     cTGTCCATGGAACCCCAGCGAAAAACCGTTCTTATTTCCCAGGGCCCCATACCCATATGCCCGCCCCCCCCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGc                                                   <  1:1116084/239‑1 (MQ=255)
                                                                                                                                                                                       gTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCCCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTTGCGACCGCGTTACTTTTGCGACTATCCGGGGGGTTCCAGGCTAACAGGGCAt                                                 >  2:533641/1‑239 (MQ=255)
                                                                                                                                                                                        ccgcggccAACCAAACGAGAAACCGTTTGGATTACCCAGTGGCCCATCCCCATAGGACCGCCCCCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCAtt                                                <  1:885165/232‑1 (MQ=255)
                                                                                                                                                                                          ccTGGCACCCCAACGAGAAACCTTTCTGATTTCCCAGTGCCCCATCCCCATAGGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGt                                              <  1:292411/238‑1 (MQ=255)
                                                                                                                                                                                                     aaCGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATACCTCGTTTCACCCGTAACTCATCTAACACCTGACGCAAATTCCCCACCCCTTTGGGGACCCCGTTATCTTTGCTGCTATCCGGGGGGTTCGCGGCTAATCGGGCATTGTATTCATAAc                                     >  2:1053102/1‑237 (MQ=255)
                                                                                                                                                                                                      aCGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCt                                                                                                                                                                                            >  1:590527/1‑85 (MQ=255)
                                                                                                                                                                                                                 ctcttgttcaCCAGGGGCCCATCCCCATAGGACCGCCACCCGCCCACCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGt                       <  1:270182/230‑1 (MQ=255)
                                                                                                                                                                                                                  tCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGtt                      >  1:1150180/1‑239 (MQ=255)
                                                                                                                                                                                                                         tACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGGATTCATAACGCTTCGTCACCAGTTGCTGTTg               >  1:1167438/1‑239 (MQ=255)
                                                                                                                                                                                                                             ggTGCCCCATACCCATATGACCGCCACCCCCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGt           <  1:1291871/238‑1 (MQ=255)
                                                                                                                                                                                                                              gTGGCCCATACCCATATGACCGCCACCACCTCAGCAGCCGTAGCCCATGCCCATTCCGGCGCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCCAATTCTCCATCTCTTTTGCGACCGCGTTAATTTTGCTGTTATACGGTGCGTTCGCGGCTACCAGGGCTTTGTATTCATAACGCTTCGTCACCCATTTCTTTTGCAggg          >  2:905002/1‑237 (MQ=255)
                                                                                                                                                                                                                                ggCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATaa                                      >  1:166434/1‑209 (MQ=255)
                                                                                                                                                                                                                                ggCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGGATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTgc         >  1:23296/1‑238 (MQ=255)
                                                                                                                                                                                                                                ggCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAAACGGGCCTTGTTTTCATAACCCTTCGTCACCATTTCCGGTTgagggggg        >  2:1164465/1‑232 (MQ=255)
                                                                                                                                                                                                                                ggCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCAATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGCAACCAGTTGCTGTTGCACTgc         >  1:583732/1‑238 (MQ=255)
                                                                                                                                                                                                                                 gCCCATCCCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTgcg        <  1:1109617/238‑1 (MQ=255)
                                                                                                                                                                                                                                      tacccataTGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCtt  >  1:312687/1‑239 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                             
GCTGGCATGCGATGGAACAGACCGTGACCACCTGCGAAGGGCAAACCATTAAACCTGATTTAGATTCGCAGGCGATAGCGCATATCGAGCGCCGCCAGAGCCGCTCTTCTGTTGATGTCAGCGTGGCATGGCTGGAAGCGCCCGAAGGTTCGCAATTACTGTTAGTGGCAAACTCTGATTTCTGTCGCTGGCAACCCAACGAGAAAACGTTCTGATTTACCAGTGGCCCATACCCATATGACCGCCACCACCGCAGCCGCCGTAGCCCATGCCCATTCCGGCTCCGCGCGGAATACCCGCTTCAGCCATCGCGATATCTCGTTTCACCCGTAACTCATCTAACGACTGACGCAAATTCTCCATCTCTTTGGCGACCGCGTTAATTTTGCTGCTATCCGGTGGGTTCGCGGCTAACAGGGCATTGTATTCATAACGCTTCGTCACCAGTTGCTGTTGCAGTGCGCTGCTT  >  NC_000913/4201047‑4201515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: