Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 437,444 A→C 18.6% V231G (GTG→GGG)  yajO ← 2‑carboxybenzaldehyde reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913437,4440AC18.6% 137.4 / 2.6 67V231G (GTG→GGG) yajO2‑carboxybenzaldehyde reductase
Reads supporting (aligned to +/- strand):  ref base A (35/16);  new base C (3/13);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 9.80e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACG  >  NC_000913/437207‑437682
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              
tCGGCAATCTTTTCCGGCTTCAAAGTGATATCCACCGCATTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGacac                                                                                                                                                                                                                                               <  1:1186116/239‑1 (MQ=255)
 cGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCCACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCCCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGacac                                                                                                                                                                                                                                               >  1:823666/1‑238 (MQ=255)
           ttCCGGGTTCCAATTTGTATCCCCCGGGTTAGATGGCCCAACGAGGTGTTCCTCGCGGCAAGTGCCGATAATCGGGTGCGCAATGCCCGGTTTAATCCACAACCAGGCCAGCGCAACTTGTGCCCGTGTCGCCCCCAGTTCTTCACTGCCGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCgg                                                                                                                                                                                                                                    <  2:1287842/239‑1 (MQ=255)
           ttCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCCTCGCTTTCTTTTTAGAGCTTTTTCCCCCCCTCATCAGccaccaacccagcg                                                                                                                                                                                                                                     >  1:233936/1‑230 (MQ=255)
           ttCCCGGTTCAAAGGGTATTCCCCCGGGTTCCATAGGTCATTCAGCTGTTCCTCGGGCGAAGTTCCGAAAATGCGTGCGGCAATGCCCGTTTTACTCAACACCCAGGCCAGCGCCACTTTTGCTCGTGTCGCCCCCAGTTCTTCCCTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCAGCAGACACCAGTCGTGCgg                                                                                                                                                                                                                                    <  2:583542/239‑1 (MQ=255)
                   tCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCCGACCCCAGTCGTGCGGTAGtttt                                                                                                                                                                                                                             >  1:1287842/1‑237 (MQ=255)
                     aaaGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTtttc                                                                                                                                                                                                                           >  1:925734/1‑238 (MQ=255)
                            tatCCCCCGCGTTCAATAGCCCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGa                                                                                                                                                                                                                   <  1:1100670/239‑1 (MQ=255)
                                 aCCGCGTTCAATCGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGt                                                                                                                                                                                                              <  1:243623/239‑1 (MQ=255)
                                   cgcgTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGCGGTTTTTCCCACCCTCTTCAGACACCAATCGTTCGGCATTTTCTCCACACGGAccggcaa                                                                                                                                                                                                            >  2:301597/1‑233 (MQ=255)
                                   cgcgTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTTCCCCCACGGACCCTTCa                                                                                                                                                                                                            >  1:1191500/1‑239 (MQ=255)
                                     cgTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGGGATTTTTCCCCACCTCATCAGACCCCAGTCGTGCGGTCGTTTCTCCCCACGGACGGCTCAg                                                                                                                                                                                                           >  1:1189357/1‑238 (MQ=255)
                                     cgTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGa                                                                                                                                                                                                          <  1:732837/239‑1 (MQ=255)
                                     cgTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTAACTGACGCCTGTTAACCGCTCTGCGATCTCCGCGTCATTTTCATCGCTTTCTTTATAGAGGTTTTTCCCCACCTCATCAGACACCAGACGTGAGGAGATTTTTCCCCACGGAAGCGTCaaa                                                                                                                                                                                                          >  2:243623/1‑237 (MQ=255)
                                           attgCTCATCAAGCTGTTCTTCGCGCTAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGccc                                                                                                                                                                                                    <  1:794322/236‑1 (MQ=255)
                                               cTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCtt                                                                                                                                                                                                 >  1:36081/1‑238 (MQ=255)
                                                    caAGCTGTTCTTCGCGCGAAGATCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGCTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGCCATTTTCCTCGCTTTCTTTCTAGAGATTTTTCCCCACCTCATCAGGCACCAGTCGCGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCaac                                                                                                                                                                                           >  2:274792/1‑237 (MQ=255)
                                                         tGTTCTTCGGGTGAAGTTCCGATAAGCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCggg                                                                                                                                                                                        <  1:96138/237‑1 (MQ=255)
                                                            tcttcGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGTCACCAGTCGTGCGGTAGTTGCTCCCCACGGACGCGCCAGACGGCCCCTTTCCAGCGGGCTCCa                                                                                                                                                                                   >  1:270782/1‑239 (MQ=255)
                                                                                aaTCGGGGCGGCAAGGCCCGGTTTCCTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCcct                                                                                                                                                               <  1:1258792/239‑1 (MQ=255)
                                                                                  cccgggggggCAAGGCCCGGTTTACACACAACCCGGGCCCGGGCAACTTGGTCTCGTGTACCCCCCGGTTTTTCCCTGCCGCCTGTTAACCGCTCTGCGACCTGCGCGTCATTTTCACCGCTTTCTTTATAGAGATTTTTCCCCCCCTCATCAGACCCCAGTCGGGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCcctc                                                                                                                                                              <  2:1239381/231‑1 (MQ=255)
                                                                                   cGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCCGTCGTGCGGCAGTTTCTCCCCACGGACGCGTCCGACGGCCCCTTTCCACCGGGCTCCATGGAATTACCGCcccccccccc                                                                                                                                                             >  2:853887/1‑238 (MQ=255)
                                                                                       tGCGGCAATGCCCGGTTTACTCAACAACCCGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCACACGGGCTCCATGGAATTACCGCACCGCCTCCCTGAt                                                                                                                                                         >  1:419903/1‑239 (MQ=255)
                                                                                          ggCATTGCCCGGTTTACTCAACAACAGGGCCCGCGCAACTTGTGGTGGGGTCGCCCCCATTTCTTCACGGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCCCCTCATCAGACCCCAGTCGTGCGGTAGTTTCTCCCCCCGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATaa                                                                                                                                                       <  1:713312/238‑1 (MQ=255)
                                                                                          ggCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTCCCCGCTCTGCGCTCTGCGCGTCCTTTTCCTCGCTTTCTTTCTCGAGATTTTTCCCCACCTCATCCGCCCCCAGTCGTGCGGTCGTTTCTCCCCCCGGCCGCGTCAGCCGCCCCCTTTCCAGCGGGCCCACTGAAATAACCGCAACGCCCCCCTAATAac                                                                                                                                                      >  2:73690/1‑239 (MQ=255)
                                                                                            cAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGCCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGCAGTTTCTCCCCCCGGACGCGTCAGACGGCCCCTTGCACGCCGGCCCCATGTAATTACCGCCACGCCCCCCCGAAAAaaca                                                                                                                                                   >  2:1319969/1‑239 (MQ=255)
                                                                                             acttccggtttttCTCAACAACCGGGCCCGCGAATCTTGGCCCTGGGGCGCCCCGAGTTCTGTACTGCCGCTTTTTAACCTCTCGGCGTGCGGCGCTGAATTTTCAGCGTTTTCTTTAGGAAGATTTTTCCCCCCCGTCGCCGCCCCCGGTCGTGCGGTAGTTTCTCCCCCCGGACGCGTCAGACGGCCCTTGGCCCGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAacac                                                                                                                                                    <  2:1189357/229‑1 (MQ=255)
                                                                                                          cTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCCGATAACACAGTGGAAGCAtct                                                                                                                                       >  1:336501/1‑238 (MQ=255)
                                                                                                           tCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCAtctc                                                                                                                                      >  1:115060/1‑238 (MQ=255)
                                                                                                             cacaccCCGGCCCGCTCAACTCGGTCTCGTGTCCCCCCCATTTTTTCACTGACGCCTGTTACCCGCTCCTCGATCTGCGGTTCATTTTCATCTCTTTCTTTATAGGAATTTTTCCCCCCCTCCTCAGACACCAGTCGGGCGGGAGTTTCTCCCCACGGACCCGTCAGCTCGCCCCTTGCCGGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTcgcg                                                                                                                                   <  2:493216/238‑1 (MQ=255)
                                                                                                                  ccAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGCTAACACAGTGGTAGCATCCCGCGccct                                                                                                                               >  1:297485/1‑238 (MQ=255)
                                                                                                                          gcgcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGGGGTAGCATCTCGCGCTCTTCTTCACGa                                                                                                                      >  1:731583/1‑239 (MQ=255)
                                                                                                                           cgcAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACggt                                                                                                                     >  1:768544/1‑237 (MQ=255)
                                                                                                                              acttttttcttgtgtCCCCCCCTTTTCTTCCCTGGCGCTGGTTAACCGCTCTGCAGTCTGCGCTTCATTTTCATCGCTTTTTTTATAGGTATTTTTCCCCCCCTCATCAGCCCCCGGTCGTGCGGTAGTTTCTCCCCCCGGACGCGTCAGACGGCCCCTTGCAAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATaaa                                                                                                                  <  2:115060/228‑1 (MQ=255)
                                                                                                                                cTTGGGCTCGTGTCCCCCCCAGTTTTTCACTGACCCCTGTTAACCGCTCTGCGTTCCGCGCGTCATTTTCACCGCTTTCTTTATAGAGATTTTTCCCCCCCTCATCAGACCCCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCcct                                                                                                                                                               <  2:1069962/192‑1 (MQ=255)
                                                                                                                                        cGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCGGATTGTa                                                                                                        >  1:265281/1‑239 (MQ=255)
                                                                                                                                        cGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCCCGCTCTTCTTCACGATAAATCAGATTGTa                                                                                                        >  1:1287916/1‑239 (MQ=255)
                                                                                                                                         tgttcccccctATTTTTTCTCTGACCCTTTTAAACCGCTTTGCGATCTGCGCGTCATTTTCATCGTTTTTTTTATAGAGTTTTTTCCCCCCCTCATCAGACCCCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAg                                                                                                       <  2:925734/233‑1 (MQ=255)
                                                                                                                                         gtgtCGCCCCCAGTTCTTCCCTTACGCCTTTTAACCGCTTTGGGATCCGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAg                                                                                                       <  1:440422/239‑1 (MQ=255)
                                                                                                                                         gtgtCCCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAg                                                                                                       <  1:957993/239‑1 (MQ=255)
                                                                                                                                          tgtCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAaca                                                                                                                                                     >  1:1069962/1‑192 (MQ=255)
                                                                                                                                          tgtCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAACTCAACTTGTa                                                                                                        >  1:336703/1‑237 (MQ=255)
                                                                                                                                          tgtCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGCGGTAGCATCTCGCGCTCTTCTTCACGATAAAACAAATTGAAgg                                                                                                      >  2:957993/1‑238 (MQ=255)
                                                                                                                                          tgtCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTACTGATAAAACAGTGGTAGCATCTCTCCCCCTTCTTCACGCACAATCAAATTGTaat                                                                                                      >  2:795538/1‑237 (MQ=255)
                                                                                                                                              gCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGCTAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt                                                                                                   >  1:800825/1‑238 (MQ=255)
                                                                                                                                              gCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCAAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGAt                                                                                                   >  1:878726/1‑238 (MQ=255)
                                                                                                                                                   cattttttcACTGACGCCTTTTACCCGCTCGGCGATCTGCGCGTAATTTCCATCGCTTTCTTTATAGAGATTTCCCCCCACCTCACCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa                                                                                             <  1:73690/233‑1 (MQ=255)
                                                                                                                                                   cAGTTCTTCCCTGACGCCTGTTACCCGCTTTGCGTTCTGCGCGTCATTTTCACCGCTTTCTTTATAGAGATTTTTCCCCCCCTCATCAGACACCAGTCGTGCGGTGGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa                                                                                             <  1:573045/239‑1 (MQ=255)
                                                                                                                                                     ttttttcTCTGCCCCCTTTTACCCGCTCTGCGATCTGCCCGTCATTTTCCGTGCTTTCTTTATACAGATTTTCCCCCCCCGCAGCAGACCCCAGTCGTGCGGTAGTTTCCCCCCACGGACGCGTCACACGGACCCTTGCCAGCGGGCTCCATGGAATTCCCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATa                                                                                           <  2:36081/235‑1 (MQ=255)
                                                                                                                                                     gTTCTTCACTGCCTCCTGTTAACCGTTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCCCCTCATCAGCCCCCAGTCGTGCGGTAGTTTCCCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATa                                                                                           <  1:1206007/239‑1 (MQ=255)
                                                                                                                                                           cACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCCTGGACTTACCGCCACCCCCTCCTGATAACCCAGTGGAACCATCTCGCCCTCTTCTTCCCGACAAAACAGAATGAAGGGATCCTGCCAACTTACa                                                                                     >  2:631068/1‑239 (MQ=255)
                                                                                                                                                                aCGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCTCCTTGCCAGCGGGCTCCCTGGAATTCCCCCCACGCCCTCCTGTTAACACAGTGGTAGCATTCCGCGCCCTTCTTCCCGATAAACCAGATTGTAGTGATCCTGCACACTGACAAACt                                                                                 >  2:939430/1‑238 (MQ=255)
                                                                                                                                                                aCGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGCCAAACt                                                                                 >  1:1198443/1‑238 (MQ=255)
                                                                                                                                                                    cTGTTACCCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCGCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTg                                                                                                     <  1:1248958/214‑1 (MQ=255)
                                                                                                                                                                       ttAACCGCTCTGGGATCTGCGCGCTATTTTCATCGCTTTCTTTATAGGAATTTTTCCCCACCTCATCAGACCCCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAg                                                                         <  1:301597/239‑1 (MQ=255)
                                                                                                                                                                            cGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCCCGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTg                                                                    <  1:853887/239‑1 (MQ=255)
                                                                                                                                                                              ctctGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCTAACTGGAAAACTGCGCCCAGCCGTGCt                                                                  >  1:1096411/1‑239 (MQ=255)
                                                                                                                                                                              ctctGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCCCTTCTTCACGATAAATCCGATTGTAGTGATCCTGCATACTGCCAAACTGCGCCCAccccg                                                                     >  2:99010/1‑234 (MQ=255)
                                                                                                                                                                                      tcggcgcCTTATTTTCATCGCTTTCTTTAAAGAGATTTTTCCCCACCTCATCAGACCCCAGTCGTGCGGTAGTTTCCCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTg                                                           <  1:776035/235‑1 (MQ=255)
                                                                                                                                                                                       ttggccgTCCTTTTCCTCCGTTTCTTTAAAGAGATTTTCCCCCCCCCCAACACACCACGGTCGTGCGGTAGTTTCCCCCCACGAGCGCCTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGa                                                         <  2:1287916/234‑1 (MQ=255)
                                                                                                                                                                                       ttgccgcttaTTTTCTTCGTTTTTTTTATGGAATTTTTCCCCCCCCCCATCAGCCCCCAGTCGTGCGGTTGTTTCCCCCCCGGGACGCGTCAGGCGGCCCCGTGCCAGCGGGCTCAATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCGGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGa                                                         <  2:265281/230‑1 (MQ=255)
                                                                                                                                                                                         gcgcGTCATTTTCATCGCTTTCTTTTTAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCa                                                                                             >  1:266011/1‑201 (MQ=255)
                                                                                                                                                                                          cgcgTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGt                                                       >  1:626310/1‑238 (MQ=255)
                                                                                                                                                                                               cATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGt                                                 >  1:1118420/1‑239 (MQ=255)
                                                                                                                                                                                                                 taaaggaaTTTTCCCCCCCCCCATCAAACCCCAGTCGGGCGGTAGTTCCCCCCCCCGGCCGCGTCAGACGGCCCCTTGCCAGCGGGCTCAATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGaa                               <  1:795538/232‑1 (MQ=255)
                                                                                                                                                                                                                         tttttCCACAGCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCTCACGGACGCGTCAGACGGCCCATTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGCTAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCTTGCATACTGACAAACTGACACCAACCGAGATGTTTTTGGAGCTCCAGTGCCGGAGCAAACTGCGAAACggacat                       >  2:1256575/1‑234 (MQ=255)
                                                                                                                                                                                                                                 cACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGCTAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGGGCTTGTTTTGGAGTTCCAATCCCTGAGCAACCTGCCAAGCGGCCATTGGTGAcc               >  2:1160711/1‑238 (MQ=255)
                                                                                                                                                                                                                                  aCCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGGCCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACGGCGAAGGGGCCATTGTTGAcgc              >  2:9555/1‑239 (MQ=255)
                                                                                                                                                                                                                                       atcaGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGa         >  1:476100/1‑239 (MQ=255)
                                                                                                                                                                                                                                        tcaGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGTTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTgcg                                                                              <  1:382252/169‑1 (MQ=255)
                                                                                                                                                                                                                                              acCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGGGGTAGCATCTCGCGCTCTTCTTCACGCTAAATCAGATTGTAGTGATCCTGCATACTGCCAAACTGCGCCCAGCCGTGCGGTTTTTGGAGTTCCAGTGCCCGGGCAAACTGCGAAGCGTGCATTTAGGACGCCCCGagaaaaac  >  2:625258/1‑232 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              
TCGGCAATCTGTTCCGGCTTCAAAGTGATATCCACCGCGTTCAATAGCTCATCAAGCTGTTCTTCGCGCGAAGTTCCGATAATCGGTGCGGCAATGCCCGGTTTACTCAACAACCAGGCCAGCGCAACTTGTGCTCGTGTCGCCCCCAGTTCTTCACTGACGCCTGTTAACCGCTCTGCGATCTGCGCGTCATTTTCATCGCTTTCTTTATAGAGATTTTTCCCCACCTCATCAGACACCAGTCGTGCGGTAGTTTCTCCCCACGGACGCGTCAGACGGCCCCTTGCCAGCGGGCTCCATGGAATTACCGCCACGCCCTCCTGATAACACAGTGGTAGCATCTCGCGCTCTTCTTCACGATAAATCAGATTGTAGTGATCCTGCATACTGACAAACTGCGCCCAGCCGTGCTGTTTTTGGAGTTCCAGTGCCTGAGCAAACTGCGAAGCGTGCATTGATGACGCGCCGATATAACG  >  NC_000913/437207‑437682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: