Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,344,984 | A→C | 15.9% | T90P (ACC→CCC) | rpoN → | RNA polymerase, sigma 54 (sigma N) factor |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,344,984 | 0 | A | C | 15.9% | 182.1 / 3.5 | 83 | T90P (ACC→CCC) | rpoN | RNA polymerase, sigma 54 (sigma N) factor |
Reads supporting (aligned to +/- strand): ref base A (27/38); new base C (4/13); total (31/52) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.62e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCG > NC_000913/3344747‑3345215 | caacaaCGGGCGATGACGCCACAGCTCCAACAGGCAATTCGGCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGaca < 1:741893/238‑1 (MQ=255) caacTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGACCAAAAAGAGATGCCGGAAGGCCTGCCGCCCGATGCCAGTTTGGAACCCAt > 1:778109/1‑239 (MQ=255) cTGGCGATGACGGCACAGCTCCAACAGGCAATTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTa < 1:858903/239‑1 (MQ=255) tgggggtGACCCCACAGCTCCACCGGGCAATTCGCGGGTTGCAGTGTTCGACGCTGGACCTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTac < 2:1266835/233‑1 (MQ=255) ggggggtGACGCCACAGCTCCACCTGGCAATTCGTCTGTGGGGGTTGTCGAGGCGGGAACTTCAGCAGGAGCTACAGCAGGCGGTGGAGAGTAAACCGCGTCTTGAGCAAATCGACACGCAGGAAGAAATCGACCCCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTac < 2:637260/233‑1 (MQ=255) gCGGTGGCGCCCCCGCTCCACCAGGGAATTCGTTGGTGGCAGGTGTCGGCGCTGGAACTGCAGGAGGAACTCCCGGAGGTGCCGGGCAATAATCCGCTGCTGAAGGAAAACGGCACCCATGAAGAAACCGGAGCCCGGGAAACGAGAGACAGGGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACCCCATTTacac < 2:863913/239‑1 (MQ=255) gCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCAGTTGTCGACGCTGGCACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCCCGAACAAAAAGAGAGCCCGGAAGAGATGCCGCTCGATACAAGTTGGGAACCCATTTaaa > 1:565210/1‑236 (MQ=255) gCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACCCAATTTcca > 1:1265080/1‑238 (MQ=255) gCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGACCTGCCCCTCGAGGCCAGTTGGGCCACCATTTaca > 1:592696/1‑238 (MQ=255) gCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCACGAACAAAAAGAAATGCCGGAAGAGCGCACGCCCAATGCCAGTTGGGACACCATTTaca > 1:1035875/1‑238 (MQ=255) ggttGACCCCCCAGCCGCAACAGGAAATTCGGGTGTTGCAGGTGGCGCCGCTGGAACTGCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAACCCGCGGCTTGAGAAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACGGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTacac < 2:1283995/235‑1 (MQ=255) cGATGACGCCACAGCTCCAACAGGCAATTCGTCCGTTGCAGTTGCCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTacac < 1:173542/238‑1 (MQ=255) ccccACAGCTCCAACAGGCAAGTCGTCTTGTGCAGTTGGCGACGATGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGTCACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTg < 1:914807/235‑1 (MQ=255) tCCAACAGGCAATTCGTCTGTTGCAGTTGTCGGCGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCa < 1:582801/236‑1 (MQ=255) tCCAAAAGGAAATTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATcc < 1:208644/239‑1 (MQ=255) ccccacgggCAATTCGGTGGTTGCCGTTCGCCCCGGTGGAACATCAGCAGGGGCCACAGCAGGCGCTGGAGAGTAAACCGCTGCTTGAGCAAATCGACACCAATGAAGAAATCGGCCCCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATcc < 2:226417/232‑1 (MQ=255) cAACAGGCAATTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACCCCATCCg > 1:1308008/1‑238 (MQ=255) cAATTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCCGTTGGGCCACCATTTACACCGCTGGTACACCATCCGGCACCAGc > 1:523988/1‑239 (MQ=255) aaTTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCAtttcaa > 1:907678/1‑209 (MQ=255) aaTTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAAAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGCCCCCAGCg > 1:1105077/1‑239 (MQ=255) ttCGTCTGTTGCAGTTGTCGACGTTGGACCTTCAGCAGGACCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGt < 1:829081/239‑1 (MQ=255) ttCGTATGTAGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAACCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGt < 1:518153/239‑1 (MQ=255) tGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCACCGGTGACTACAtt > 1:793470/1‑239 (MQ=255) ttgcGACGCTGGAATCTAGGCAGGAGCTACACGAGGGCCTGGAGAGTAATCCGCTGCTTGGACAAATCGACCCTCATGAAGAAATCGACCCCCGCGAAACGCAAGACAGTGAAACGCTGGACCCCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTgac < 2:824054/233‑1 (MQ=255) tGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGGACCCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTCCATTgaagac > 1:597801/1‑239 (MQ=255) gTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGacgac‑g > 1:977630/1‑239 (MQ=255) aagggggAACTTCCGCAGGGGGTACAGCAGGGGCTGGAGGGGAACCCGTCGCTTGAGCAAATCGACCCCCATGAAAAAATCCGCCCCCGCGAAACGAAAGACAGTGAAACGCGGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCt < 2:288230/234‑1 (MQ=255) gcgggAACTTCAGCAGGGCCTTCAGCGGGCGGTGGGGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGGGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCCTCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGc < 2:944319/236‑1 (MQ=255) tGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGAAACCGCCGACGCGCTCGAACAAAAAGAGAGGCCGGAAGAGCTGCCGCCCGCGGCCCGTTGGAACACAAATTAAACCCCTGGCACACCCCCCGGACCCCCCGGTGGCCACATTGCAGAC‑CAGCGGCCg > 2:1061777/1‑239 (MQ=255) gAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGATACGCTGGACACCGCCGACGCGCGCGAACAAACAGAAATGCCGGAAGAGCTGCCGCCCGATGCCAGCTGGGACACCATTTAAACCGCTGTCACCCCAACCGGTACCCGCGGGGAATCCATTgaggaagcgcaggcgg > 2:138111/1‑227 (MQ=255) gAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTAAACCATCCGGCACCAGCGGGGACTAACTTTACCAC‑GAGCTTCCgg > 1:817141/1‑238 (MQ=255) cTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAACAAATCGACACTGATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGaaa < 1:593388/238‑1 (MQ=255) cagcggGGGCTGGAGGTTAACCCCGTGCTTGGACAAATGGAACCCCATGAAAAAATCGACACCCGCGAAACCCAAGACAGGGAAACGCGGGGCGCCCCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACCCCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAacgac < 2:1113981/239‑1 (MQ=255) aggaggtGGAGAGTAATCCGCTGCTTGCGCAAAGCGACACTCATTAAGAAACCGAACCCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCa < 1:685709/232‑1 (MQ=255) agGCGCTGGAGAGTAAGCCGCTGCGTGAGCAAATCGACACTCAGGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCa < 1:58998/238‑1 (MQ=255) agGCGCTGGAGAGTAAACCGCTGATTGACACAATCGACACTCATGAAGAAATCGACCCCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGc < 1:740394/237‑1 (MQ=255) ggCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAg > 1:841260/1‑238 (MQ=255) ggCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGATGCCGCCCCATGCCAGTTGGGACACCATTTACACCGCTGGTACACCCTCCGGCACCAGCGGTGACAACATTGACCACAGAG‑TGCCGGGGTACCGGGGCGaaaaagccaaa > 2:716866/1‑230 (MQ=255) gcgcTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCCCCAACGGTGACCACATTGTCGCA‑GAGCATCCGGCCTACCAGGGCGAAACCACGCaaa > 2:119545/1‑236 (MQ=255) tgggggattcaTCCGCTGTTTAGGCAAAGCACCATGCATGAAAAAACCGCCCCCCCGAAAAAGCAAGCAAGTGAACCGCTGGCCCCCGCCGACCCGCTCGACCAAAAAGGGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCtt < 2:977630/229‑1 (MQ=255) tCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGCCGAAACGACGCAGACCTTCCAGGATAAc > 1:510831/1‑238 (MQ=255) ccGCTGCTTGAGCAAATCGACACTAATGAAGAAATCGACACCCGGGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGa < 1:174194/233‑1 (MQ=255) cGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCCTCCGGCACCAGCGGTGACTCCATTTACGAC‑GAGCTGCCGGTCTACCCGGGAGAACAGCAGCAGACCTTGCAGGAGTAcccg > 2:941229/1‑237 (MQ=255) ctgctTGAGCAAATCGACCCTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGAt < 1:237601/239‑1 (MQ=255) ctTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGGGACTACATTTACGCA‑GGACTGCCGGCTTACCAGGCGGAAACGAACACGACCTTGCAGGATTACCTGAttgg > 2:237601/1‑236 (MQ=255) aGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGATGGTAAACCCTCCGGCACCAGCGGGGAATACATTGACGCC‑GAGCTGCCGGACTACCAGGGCGCAACGACGCAACCACTGCAGGGTTAcccaattactg > 2:174194/1‑228 (MQ=255) acTCATGAAGAAAGCGACACCCGCGAAACGCAAGACAATGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGac < 1:660051/239‑1 (MQ=255) aacaagaaACCGACACCCGCGAAACGCAAAACAGTGAAACGCTGGACACCGCCAGCGCGATCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGACAGTTGGGACACCATTTACCCCGCTGGTACCCCATACGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGTCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACGCCg < 2:838823/236‑1 (MQ=255) aTCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑CAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTTAGC‑TGTGACCGGTTTCCg > 1:360951/1‑238 (MQ=255) cGACCCCCGCGAAACGCAAGGCAGTGAAACGCTGGCCACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGaca < 1:133112/239‑1 (MQ=255) cacCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTg < 1:321292/239‑1 (MQ=255) ccGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTCCCAGGGCGGAACGCCGCAGACCTTGCAGGATTACCTGATGTGGCAGGGTGTGC‑CGACACCGCTTTTCcacaaactac > 2:321292/1‑233 (MQ=255) cgggAAACGCAAAACAGTGAACCGCTGGACCCCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACcg < 1:1040287/236‑1 (MQ=255) cgcgAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACcg < 1:925663/239‑1 (MQ=255) gagAAACGCAAGAAAGTGAAACGGTGGACCCCGCCGACGCGCCCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACTCCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACcgc < 1:1221214/237‑1 (MQ=255) cgAAACTCAAGACAGGGAAACGCTGGACCCCGCCGGCGCCCTCGAACAAAAAGAGATGCCGGAAGGGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACcgcg < 1:181635/239‑1 (MQ=255) aaaaCGCAAGCACGTGAAACGCTGGACCCCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCgcgc < 1:9687/238‑1 (MQ=255) gACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTaca < 1:776458/102‑1 (MQ=255) gACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTaca > 2:776458/1‑102 (MQ=255) gACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTCCCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTTAGC‑TGACAACGGTTTCCGACACTGACCGCGCTATTTCtc > 1:385423/1‑237 (MQ=255) tGAAACGCTGGACACCGCCGACGCGCTCGACCCAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGCCGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGGGC‑TGACACCGTTTTCCGCACCTGACCGCGCCATTGCCAccctc > 1:379674/1‑235 (MQ=255) tGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGGTGAGC‑TGACACCCCTTTCCCGCACTGACCGCCCTGTTTGTACCTCTAttcccgat > 2:962419/1‑232 (MQ=255) cgtcgACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAgg < 1:1174003/155‑1 (MQ=255) ccgtctaaCAAAAAAGGAGGCCGGAGGAGCGCCCCCCCGATGCCAGTTGGGACCCCATTTACACCGCTGGCACCCCATCCGGCCCCAGCGGTAGCTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGCCCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAAcc < 2:804735/233‑1 (MQ=255) gcTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAAcc < 1:1061777/238‑1 (MQ=255) gcTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAAcc < 1:755690/238‑1 (MQ=255) tcccGGGAGGGCCGCCGCCCGAAGCACGTTGGGACCCCATTTCCACCGCTGGTACACCATCCGGCCCCAGCGGTGACTACATTGGCGAC‑GGTCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTccc < 2:1308008/236‑1 (MQ=255) gCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCCGGGTGAGCGGGAACCCGTTTCCCGAGCCT‑AACGAGCTATTTCTACCCCTCTCGCCGCTGCCGTTGTTGAAACCGGTTATTTGCCTGGcccac > 2:1056313/1‑237 (MQ=255) gaggcgCCGCTCGATGCCTGTTGGGACCCCAATTAAACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGAGCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAg < 1:234651/233‑1 (MQ=255) tGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTGCCGCTGGGAGATAtt > 1:781358/1‑239 (MQ=255) ccccctccgttGCCAGTGGGGACCCCATTTCCACCGTGGGTACCCCCTCCGGGACCAGCGGGTCCTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGAGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATAtt < 2:592696/229‑1 (MQ=255) gCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCCATCGTCGATGCCGTTGATGAAACCGGTTCTCTGAATGTCCCCCGGGAAgcaatt > 1:1172074/1‑233 (MQ=255) cgttCGATGCCAGTTGGGACCCCATTTACACCGGTGGTACACCATCCGGCACCAGCGGTGACTACATGGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATtctc < 1:993378/236‑1 (MQ=255) cGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACCCCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATtct < 1:1126730/238‑1 (MQ=255) ccccgttGCCATTTGGTAACCCTTTTACCACGCTGGTACAACATCCGGGACCAGCGGGAACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCg < 2:911132/233‑1 (MQ=255) cccGATGCCAGTTGGGACCCCATTTACACCGCTGGTACCCCATCCGGCACCAGCGGTTACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGa < 1:39985/237‑1 (MQ=255) cTCGATGCCAGTTGGGACCCCATTTACCCCGCTGGTTCCCCCTCCGGCACAGGCGGGTACTACATTGACGAC‑GAGCTGCCGGACTACCAGGGGGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGa < 2:251830/239‑1 (MQ=255) cTCGATGCCAGTTGGGACCCCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGa < 1:25380/239‑1 (MQ=255) ctgATGCCAGTTGGGTCACCATTTACCCCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGaa < 1:1244559/237‑1 (MQ=255) gCCAGTTGGGACCCCATTTACCCCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGtat < 1:220709/239‑1 (MQ=255) gCCAGTTGGGACACCATTTACCCCGCTGGTACCCCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGtat < 1:962419/239‑1 (MQ=255) tgTTGGGACCCCATTTCCCCCCGTGGTACCCACTCCGGGCCACGAGGTGACGACATTGACGAC‑GAGCGGCCTGTCTAACAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGAGGGTGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTTCCGCTGGACGATACTCTCGAAAGTAAAgg < 2:1249449/238‑1 (MQ=255) tGGGACACCATTTCCCCCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCg < 1:941229/238‑1 (MQ=255) tGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCg < 1:924385/238‑1 (MQ=255) | CAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCAGTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATGAAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAAGAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGAC‑GAGCTGCCGGTCTACCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTTGAGC‑TGACACCGTTTTCCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGAAACCGGTTATCTGACTGTCCCGCTGGAAGATATTCTCGAAAGTATAGGCG > NC_000913/3344747‑3345215 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |