Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,460,787	T→C	15.5%	F324S (TTC→TCC)	gspF →	general secretory pathway component, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,460,787	0	T	C	15.5%	167.0 / 3.6	65	F324S (TTC→TCC)	gspF	general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base T (48/5);  new base C (2/10);  total (50/15)
Fisher's exact test for biased strand distribution p-value = 9.30e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGA  >  NC_000913/3460556‑3461016                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        ccATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑Cccgccg                                                                                                                                                                                                                                 >  1:355308/1‑239 (MQ=255)    tGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TACCAGCATTCATGTTTCGCTGGAACAAACCGC‑AAT‑TTT‑Cccgccgcgg                                                                                                                                                                                                                              >  1:342983/1‑236 (MQ=255)    tGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCACCCGATAGCCTCCACAACCTGGCAAT‑T‑CGCCAGCGTCTGGCCAATTCGGCCGAGAACGCTAGCCCGGG‑GAAACCAATTCATCTTTCGCTGGAACAAAACCC‑AAT‑TTC‑ACCGCCgaag                                                                                                                                                                                                                              >  2:527366/1‑237 (MQ=255)        gctgcGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGAATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGaggatg                                                                                                                                                                                                                           >  1:660457/1‑238 (MQ=255)              cgTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCGGCGTCTGGCAAATTCGGCAGAGAACGTTCGCCAGGG‑TAAAAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCCCTCCa                                                                                                                                                                                                                    >  2:1031914/1‑239 (MQ=255)                ttGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGACCGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGAGGATGCTCTACAtg                                                                                                                                                                                                                  >  1:943317/1‑239 (MQ=255)                        aTCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCCCTACATGGTGGcc                                                                                                                                                                                                            >  1:1301810/1‑237 (MQ=255)                                gCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCACCAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATCCGGCAGAAACCGATCGCCAGGG‑GAACAGCATTCCTCTTTCGCTTGAAAACACAAC‑AAT‑TTT‑CCCGCCGATAATGCCCTAACTGGTGGGCTCAGGCGaaa                                                                                                                                                                                                  >  2:812817/1‑239 (MQ=255)                                gCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATCCTCTCCATGTTGGCCTCTGGCGaaa                                                                                                                                                                                                  >  1:95756/1‑239 (MQ=255)                                  tGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGaaaaa                                                                                                                                                                                                >  1:424369/1‑239 (MQ=255)                                  tGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGaaaaa                                                                                                                                                                                                >  1:641412/1‑239 (MQ=255)                                  tGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCCC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGaaaaa                                                                                                                                                                                                >  1:577786/1‑239 (MQ=255)                                                      gcACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGCAAAAAGCGGGCAGCTCGG‑CACAtt                                                                                                                                                                           >  1:775069/1‑239 (MQ=255)                                                        aCGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGAAGCTCGG‑CACATTaa                                                                                                                                                                         >  1:285093/1‑239 (MQ=255)                                                               ctcCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCTAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCGGGAACAAACCGC‑AAT‑TTT‑CCCGCCACTGTGGCCCTCCTTGTGGGCCTCTGGCGAAAAAAACGGCCTACACGG‑CCCCTTAATGGTccg                                                                                                                                                                  >  2:67602/1‑237 (MQ=255)                                                                    cACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CCCATTAATGGTCAGAGcc                                                                                                                                                              >  1:645205/1‑238 (MQ=255)                                                                     ttttttatttttttCCAACCCAGCGGTCCCCCTGGGGGGGGTGGGAT‑AATTTCGCCCCCCGAACCCCCAACCACCCCAAAATCT‑CGCCGGGGTAGGGAAAAGGCGGAAGAGAACGTTCGCCGGGG‑AAACAGCATTCTTCTTTCGGTGGAACAAACCGA‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGc                                                                                                                                                            <  2:395814/229‑1 (MQ=255)                                                                        ttAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAGCCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGCTGCTCTACATGGTGGCCTCCGGCGAAAAAAGCGGGCCGCTCGG‑CACATTAATGGTCAAAGcccccaa                                                                                                                                                         >  2:482208/1‑234 (MQ=255)                                                                         tAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGCTGAACCTCTACATTGTGGCATCTGGCGAAAAAAACGGGCACCTCGGCCACA‑TAATGTGCAGAGCCGgagaa                                                                                                                                                        >  2:1200530/1‑238 (MQ=255)                                                                         tAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAAAAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGTGGGCCACTGGGGAAAAAAGCCGGAAGCTGGG‑CACATTAAAGGTCAAACCCGCAGaa                                                                                                                                                        >  2:946827/1‑238 (MQ=255)                                                                                 ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGCAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACCGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCCCTCCATGGTGGCCTCCGGCGAAAAAAGCGGGCAGCTCCG‑GCCCTTAATGGTCGGAACCGCAGACAAACAAga                                                                                                                                                >  2:986769/1‑239 (MQ=255)                                                                                 ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACCGCATTCATCTTTCGCTGGAACACACCGC‑AAT‑TTT‑CCCGCCGATGATGCCCTACATGATGGCCTCTGGCGACAAAAACGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAggg                                                                                                                                                >  1:948369/1‑238 (MQ=255)                                                                                 ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑ACT‑TTT‑CCCGCCGATGATGCTCGACATGTTGGCCTCTGGCGAAAAACGCGTGCAGCTGGG‑CACATTAATGGTCAAAGCCGACGAAAACCAgag                                                                                                                                                >  2:1007801/1‑237 (MQ=255)                                                                                 ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTa                                                                                                                                                                                                                      >  1:1128013/1‑170 (MQ=255)                                                                                 ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGTTAACCAGga                                                                                                                                                >  1:79574/1‑239 (MQ=255)                                                                                 ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGCCAGAGCCGCCGTTAACCAgta                                                                                                                                                >  1:341038/1‑237 (MQ=255)                                                                                 ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAACGGGCAGCTCGG‑CACATTCATGGTCAGAGCCCGAGGTAACCAGga                                                                                                                                                >  1:1223302/1‑239 (MQ=255)                                                                                 ttGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGAAGAGAACGTTCGCCAGGG‑TAACAGCATTCTTCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGTCTTACATGGTGGCCTCGGGCGCACAAAGCGGGGAGCTCGG‑CCCAAGAAAGGACCGAACCGCacaaaaacaaga                                                                                                                                                >  2:731376/1‑228 (MQ=255)                                                                                                 tCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACaaaa                                                                                                                                 >  1:395602/1‑238 (MQ=255)                                                                                                 tCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGAAACTCCAACaaaa                                                                                                                                 >  1:1324562/1‑238 (MQ=255)                                                                                                 tCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGACGATGCCCAACATGGTGGCCTCTGCCGAAAAAAGCGGGCAGCCCGC‑GACCTTAAAGGTCAAAGCCACAGAAAAACAAGAGAAACCACAaaaaaaa                                                                                                                                >  2:646448/1‑238 (MQ=255)                                                                                                            gatgggatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGGGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑ACT‑TTT‑CCCGCCGATGATGCTCTCAATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑AACATTCATGGTCAGAGCCCCCGATAACCAGGAACAACACCAACAAAAACGAAACGcgta                                                                                                                     >  2:492315/1‑236 (MQ=255)                                                                                                            gatgggatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCtt                                                                                                                     >  1:689193/1‑239 (MQ=255)                                                                                                              tgggatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGAAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGACGCAAAAATCGGCCAGCTCGG‑CACATAAATGGTCAGAGCCGCAGAAACCCAGGAGACCCTCCAACAAAAACGGACCGccacaa                                                                                                                   >  2:1311749/1‑235 (MQ=255)                                                                                                                 gtagAATTTCTCCCCCAAAATCCACAAC‑ACCCCAAAAT‑TCCCCCAGCTTCTGGAAAGGGCGGCAAAAAACTTTCCCCAGGG‑AAAGGGCATTCATTTTTCGCGGGAACAACCCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACg                                                                                                                 <  1:986769/235‑1 (MQ=255)                                                                                                                 gatgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACg                                                                                                                 >  1:1209913/1‑238 (MQ=255)                                                                                                                 gaggAATTTGCCCACCAAAAGCCTAACAAACCTCGAAAT‑T‑CGCCGGCTTCGGGCAAAGGCGGAAAAGACCGTCCGCCGGGG‑TAACAGCATTCATCTTTCGGTGGAAAAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACg                                                                                                                 <  1:485123/235‑1 (MQ=255)                                                                                                                   tgAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGCTAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGct                                                                                                               >  1:299985/1‑238 (MQ=255)                                                                                                                           tCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAACAAAGCAGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCCGATAACCAGGAGACACTCCAACAAAATCGGATTGCCTTAACGCTCTCCATCtt                                                                                                      >  1:31075/1‑239 (MQ=255)                                                                                                                           tCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGAAACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCt                                                                                                       >  1:242060/1‑238 (MQ=255)                                                                                                                                     cccccaacacCCTGAAAAT‑C‑CGCCAGGGTGCGACAAAGGCGGAAGAGAACGGTCGCCAGGG‑TAACAGCATTCATCTTGCGCTGAAACAACCCGC‑AAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCa                                                                                            <  1:1031914/235‑1 (MQ=255)                                                                                                                                        tCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCACCAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCa                                                                                            >  1:698674/1‑236 (MQ=255)                                                                                                                                                   caaaattgcGCCAGCCTCTGGAAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACACGATTCTTCTTGCGCGGAAACAACCCGA‑AAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACg                                                                                <  1:588968/231‑1 (MQ=255)                                                                                                                                                                 cGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CTCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCTTAATTACGATGGCACTGATCg                                                                   >  1:1027500/1‑238 (MQ=255)                                                                                                                                                                  gTCGGGAAAACGGGGCAGAGCACTTCCGCCGGGG‑AAACCGATTCCATTTTTCGC‑GGAACAAACCCG‑AAT‑TTTCCCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTc                                                                 <  1:812817/239‑1 (MQ=255)                                                                                                                                                                     gggaaacggCGGCAGAAGCCGTCCCCAAGGG‑TAACAGCTTGCTTTGTGCGCGGAAACAACCCGA‑AAT‑GTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCt                                                               <  1:990645/230‑1 (MQ=255)                                                                                                                                                                            tGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATAGCCTTAACGCTCTCCCTCTTCGAGCCAGCCCTCATTATTACGATGGCACTGATCGTCCTGTTTAtt                                                        >  1:1034872/1‑238 (MQ=255)                                                                                                                                                                                        acgtccccAGGGATAACCGCATTCATTTCTCGGT‑GAAAAAACCCC‑AATGTTC‑CCCGCCGATGATGCCCTACATGGGTGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTa                                            <  1:65225/233‑1 (MQ=255)                                                                                                                                                                                           tCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACAATCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGCTACTcc                                        >  1:185478/1‑239 (MQ=255)                                                                                                                                                                                             gCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCa                                       >  1:1265384/1‑238 (MQ=255)                                                                                                                                                                                              ccAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAAACGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGCGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGCGCCAGCCATCATTATTACGATGGCACTGATCGTCCTGTTTATTCTCCTGTCGGCACTcaca                                      >  2:284804/1‑235 (MQ=255)                                                                                                                                                                                                        cAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCttcttc                            >  1:394918/1‑239 (MQ=255)                                                                                                                                                                                                            tttCATCTTCCG‑GGGAAAAAACCCCAAAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACt                        <  1:646448/238‑1 (MQ=255)                                                                                                                                                                                                             ttCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCCGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAAAGCTCTCCATCTTCGAGCCACCACTCATTATTACGTAGGGCTTGATCGTCCTGTTTTTTTTCGTGCCCGAATCCCAACACCTTTTTCAACtg                       >  2:335668/1‑238 (MQ=255)                                                                                                                                                                                                             ttCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGCTAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCCCTGACCGTCCTGTTTCTTGCCGTGTCGGTACTCCAACCTCTTTTTCAACtt                       >  2:1052138/1‑239 (MQ=255)                                                                                                                                                                                                             ttCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCG‑AAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTa                      >  1:1124818/1‑239 (MQ=255)                                                                                                                                                                                                              tCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCCCTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTa                      >  1:177853/1‑239 (MQ=255)                                                                                                                                                                                                                        gtgaaaaaacccGC‑AAT‑TTC‑CCCGCCGATGATGCTTTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGat            <  1:793154/229‑1 (MQ=255)                                                                                                                                                                                                                        cTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAATAAAGCGGGCAGCTCGA‑CACATTAATGGGCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGCTGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTTTTCAAATTAACTCCATGat            >  1:732434/1‑239 (MQ=255)                                                                                                                                                                                                                         ggaaaaaaaCCCA‑AAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGat            <  1:479400/232‑1 (MQ=255)                                                                                                                                                                                                                              aacacccactaT‑TCT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CAAATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGattaatt       <  2:395602/229‑1 (MQ=255)                                                                                                                                                                                                                               aaaCCGT‑AAT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCG‑AAAAAGCGGGCAGCTCGG‑CACATTAATGGGCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg    <  1:478032/239‑1 (MQ=255)                                                                                                                                                                                                                                 cctccataT‑TTC‑CCCGCCGATGAGGCTCTACATGGGGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGGCAGGGCCGCAGATAACCAGGGGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg    <  2:904617/232‑1 (MQ=255)                                                                                                                                                                                                                                 accccattt‑ttc‑CCCGCCGATGATGCTCTACCTGGTGGCCTCTGGCGAAAAAAGGGGGCAGCTCGG‑CAAATTAATGGTCAGAGCCGCAGTTAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg    <  2:79574/231‑1 (MQ=255)                                                                                                                                                                                                                                 acccagaaT‑TTC‑CCCGCCGATGATGCTCTTACTGGGGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CAGATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAg    <  1:284804/233‑1 (MQ=255)                                                                                                                                                                                                                                   ccacatt‑ttc‑CCCGCCGATGATGCTCTAAATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGa  <  1:666915/233‑1 (MQ=255)                                                                                                                                                                                                                                       aaT‑TTC‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATtg                                                       >  1:59618/1‑182 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                        CCATGTTGCTGCGCGTTGCGCTCATCGGCCCGCTGATTTGCGCCATTAACAGCGCACGCTATCTCCGCACTTTAAGTATTTTGCAATCCAGCGGCGTCCCTCTGCTGGATGGGATGAATTTGTCCACCGAAAGCCTCAACAACCTCGAAAT‑T‑CGCCAGCGTCTGGCAAATGCGGCAGAGAACGTTCGCCAGGG‑TAACAGCATTCATCTTTCGCTGGAACAAACCGC‑AAT‑TTT‑CCCGCCGATGATGCTCTACATGGTGGCCTCTGGCGAAAAAAGCGGGCAGCTCGG‑CACATTAATGGTCAGAGCCGCAGATAACCAGGAGACACTCCAACAAAATCGGATCGCCTTAACGCTCTCCATCTTCGAGCCAGCACTCATTATTACGATGGCACTGATCGTCCTGTTTATTGTCGTGTCGGTACTCCAACCTCTTCTTCAACTTAACTCAATGATTAATTAAGGA  >  NC_000913/3460556‑3461016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑