Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,527,722 A→C 15.5% S418S (TCA→TCC yrfF → putative RcsCDB‑response attenuator, inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,527,7220AC15.5% 195.8 / 3.1 98S418S (TCA→TCCyrfFputative RcsCDB‑response attenuator, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (48/29);  new base C (2/19);  total (50/48)
Fisher's exact test for biased strand distribution p-value = 1.36e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACT  >  NC_000913/3527496‑3527943
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                             
tCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGACCTTCCGGAACCTGGAGCGCGAAAACCAATTCAcc                                                                                                                                                                                                                             >  1:318120/1‑229 (MQ=255)
tCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCAAACTTCCGGAACCTGGAGGGCGAAAACCAATTAAcc                                                                                                                                                                                                                             >  2:556852/1‑229 (MQ=255)
gcGCTGCGGGCTCGCTGCTGGTTCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCAc                                                                                                                                                                                                                              <  1:289817/227‑1 (MQ=255)
        ggCTCGTTGTTGGTGTTGTTTATGCTGTTATACTGGATCCCGCTGGATATGCCGATGAAAGTCACTCTAGCAGGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTa                                                                                                                                                                                                                      <  1:747739/228‑1 (MQ=255)
              ctgctgGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTACGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTg                                                                                                                                                                                                               >  1:286876/1‑229 (MQ=255)
                             aggCTGTTATTCTGGAACCCGCTGGAGATGCCGCTGAAATTCCCTCTCGCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACTCTGGCGTGCGGGTAGGAGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGatc                                                                                                                                                                                                 <  1:477360/226‑1 (MQ=255)
                                ccttttttCTTGATGCCCCTGGATAGGGCGCTGAAATTCCATTCCTACTGGATGAAAGGCGCGCAGCCCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCt                                                                                                                                                                                             <  2:68322/223‑1 (MQ=255)
                                  gTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTg                                                                                                                                                                                            >  1:528711/1‑228 (MQ=255)
                                   ttATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTgg                                                                                                                                                                                           >  2:638154/1‑228 (MQ=255)
                                    tATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGACCTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGa                                                                                                                                                                                          >  1:16159/1‑228 (MQ=255)
                                    tATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGCCTGCTCGCAGATCATCTGGa                                                                                                                                                                                          >  1:231131/1‑228 (MQ=255)
                                    tATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGa                                                                                                                                                                                          >  1:754549/1‑228 (MQ=255)
                                           gATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGcc                                                                                                                                                                                  >  1:131097/1‑229 (MQ=255)
                                           gATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGc                                                                                                                                                                                   >  1:55515/1‑228 (MQ=255)
                                                cGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACGTTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACAACGCCCGCTc                                                                                                                                                                             >  1:57477/1‑229 (MQ=255)
                                                cGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTCCGGGAATGTGTCATATTCGACCTTCCGGAACTCGGAGCGCGAAAACCAATTCCTCTTTTTTTCAGTTTTACTCCTCGCAGGTACTCTGGAACTACGCCCGcc                                                                                                                                                                              >  2:195248/1‑227 (MQ=255)
                                                cGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGCTCATCTGGAAGGACGCCCGCt                                                                                                                                                                              >  1:445547/1‑228 (MQ=255)
                                                cGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGACTGGGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCCCCTTTTTTACCGTTTGATTGCTCGCGGATCATCTGGAACGACGCCCCCTCa                                                                                                                                                                            >  2:372390/1‑229 (MQ=255)
                                                     gATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAACACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACCTTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCTGAACCTGGAGCGCGAAAACCAATGCACCATGTTTACCGTTTGCCAGCTCGCAGACAAGCCGGAACGACCCCCCCTCAATGc                                                                                                                                                                        >  2:347913/1‑229 (MQ=255)
                                                            cggtGAAATTCCCTCTCGCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAcc                                                                                                                                                                 <  1:119716/226‑1 (MQ=255)
                                                            cgcgGAATTTCACCTTCTCATGGATGAAAGGCGCGCAGGCCATGGAATCCACCAGCGTAAAACAACTGGCTGACGCTGGGGTGCGGGTAGGCGATACATTGCGTAGTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCCCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAcc                                                                                                                                                                 <  2:55515/229‑1 (MQ=255)
                                                             cgtGAAATTCCCTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAcc                                                                                                                                                                 <  1:556853/226‑1 (MQ=255)
                                                             cgtGAAAGTCACTCGCGAAAGGAGGAAAGGCGGGCAGACCATGGAAGCCTCCAGCGTAAAACAACGGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTAcc                                                                                                                                                                 <  1:82505/226‑1 (MQ=255)
                                                               tGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGa                                                                                                                                                              >  1:479547/1‑229 (MQ=255)
                                                               tGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAACGCGAAAACCAATTCACCTTTTTTACCGTTTGCCTGCTCGCAGCACCTTTGGAACACCGCCCCCCCATTTCCATTCCCCGa                                                                                                                                                              >  2:417293/1‑229 (MQ=255)
                                                                                   aTGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCCCATTGCCATTACCAGAATCTGACATGGTAAACaaaa                                                                                                                                          >  2:656303/1‑228 (MQ=255)
                                                                                     gAAAGGGCCGCGGTCCATTGAAGCCCCCAGCTTAAACACACTGGCTGGCGCGGGGGGTCGGGTAGGCGCTACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCg                                                                                                                                        <  2:753124/229‑1 (MQ=255)
                                                                                     gAAAGGCGGGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGa                                                                                                                                       <  1:449899/229‑1 (MQ=255)
                                                                                     gAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATCCATTGCGTATTAGCGGTACGGGAATGTGTAATCTTCGAACTTCCGGCACCTGGAGCGGGACAACAAATTCACCTTTTTTACCGTTTGAATGCTCGCAGATCATCAGGAACGACGCCCGCCAATTGCCATTACCCGAACATGCACTGGGcaaaacaac                                                                                                                                         >  2:714295/1‑222 (MQ=255)
                                                                                     gAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGc                                                                                                                                         >  1:404617/1‑228 (MQ=255)
                                                                                      aaaGGCGGGCCGCCCATTGAAGCCCCCAGCTGAAAACAACTGGCTGGCGGTGGGGTGCGGGTAGGCGATACATTGCGTATTAGGGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGa                                                                                                                                       <  2:693112/229‑1 (MQ=255)
                                                                                       aaGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGATTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGc                                                                                                                                         >  1:208837/1‑226 (MQ=255)
                                                                                       aaGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGAc                                                                                                                                      >  1:347194/1‑229 (MQ=255)
                                                                                       aaGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCCCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGc                                                                                                                                         >  2:750639/1‑226 (MQ=255)
                                                                                       aaGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTCCTCGCAGCTCATCTGGAACGACGCCCGCTCATTGCCATTACCAAAATCTGAACTGGTCaaaaaaca                                                                                                                                         >  2:230793/1‑224 (MQ=255)
                                                                                       aaGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGGACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTAGCAGATCATCTGGAACGACGCCCGCTCATTTCAATTACCAGAATCTGAACTGGCCACACAAGCGAc                                                                                                                                      >  1:707757/1‑229 (MQ=255)
                                                                                        aGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGGAAACCAATTCACCTTTTTTACCGTTTGATTGATCGCACATCATCTGGAACGACGCCCGCTCATTCCCATTCCCAGAATCGAAAATGGACAACaaaaaacag                                                                                                                                     >  2:725544/1‑223 (MQ=255)
                                                                                         ggCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCACCTGGAACGACGCCCGCTCCTTGCCATTCCCAGACACGGAACTAGTCAAAAAAACAACgg                                                                                                                                    >  2:155927/1‑229 (MQ=255)
                                                                                           cgcgcAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCa                                                                                                                                  <  1:638155/229‑1 (MQ=255)
                                                                                                      tggAAGCCCCCAGCGTAAAACAACTGGCTGACGCTGGGGTGCGGGTAGGCGATCCATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGc                                                                                                                       <  2:754548/227‑1 (MQ=255)
                                                                                                       tGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGCTTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGTACTGGTCAACAAAGCGACGGCATTGCCTGAAGc                                                                                                                       >  1:36634/1‑228 (MQ=255)
                                                                                                            ccaccaGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCTAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTCCCAGAATCTGAACTGGTCAAAAAAACGACCGCATTGCCGGAAGAAGTaaa                                                                                                                 >  2:142178/1‑229 (MQ=255)
                                                                                                            ccaccaGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAAACTGAAAGGGCAAACAAAGCGACGGCATTAACTGAAGAAGGTaa                                                                                                                 >  2:431482/1‑229 (MQ=255)
                                                                                                            ccaccaGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGATCGCAGATCATCTGGAACGACGCCCGCTCATTCCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGCCTACAGCAGTTa                                                                                                                  >  1:169304/1‑228 (MQ=255)
                                                                                                              accaGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAAt                                                                                                                >  1:403786/1‑228 (MQ=255)
                                                                                                                caGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGc                                                                                                             <  1:372390/229‑1 (MQ=255)
                                                                                                                      aaaaCAACTGGTGGCCGCTGGCGTGGGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTGCGGAACCTGGAGCGCGAAACCCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTg                                                                                                       <  2:566918/229‑1 (MQ=255)
                                                                                                                              tGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATAGTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTAAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTa                                                                                                <  1:417293/228‑1 (MQ=255)
                                                                                                                                   ggaggtggggtggggGGTGGCGATACGTTGCGGATTAGCGGTACGGGGAAGTTGTATTTTCTGTCTTTCGGGAACCGGAGCCCGAAAACCAAAGTCCCCTTTTTACCCTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCGCCCTAAACCgg                                                                                          <  2:179588/224‑1 (MQ=255)
                                                                                                                                               cGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCAACGTGCACCCTAAACCGGAAAATGAAtac                                                                               >  2:124905/1‑226 (MQ=255)
                                                                                                                                               cGGGTAGGCGATACAGTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCGCCCTAAACCGGAAGATGAAtct                                                                               <  1:195248/228‑1 (MQ=255)
                                                                                                                                                ggttAGGCGATTAATTGCGTATTAGCGGTACGGGAATGGTTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTcg                                                                             <  2:16159/226‑1 (MQ=255)
                                                                                                                                                  gttggCGATACGTTGCGTATTAGCCGTACGGGAAAGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTGTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTcgc                                                                            <  1:347913/225‑1 (MQ=255)
                                                                                                                                                   tggggcttgccttGGGTTTTAGCGGTAAGGGAATGTGTAATATGCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTGTGACTGCTCGCAGATCAGCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGt                                                                          <  2:339461/218‑1 (MQ=255)
                                                                                                                                                      gcaaTACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTTGAGCGCGAAAACCAATTCCCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCa                                                                        <  1:714296/225‑1 (MQ=255)
                                                                                                                                                          tACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCACCGGCATTGACTGCAGCAGTTAATCGCCGACTGCCCCCTAACCCGGAAAATGAATCTCTCGACATTGc                                                                    >  1:652652/1‑228 (MQ=255)
                                                                                                                                                           aCATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGcc                                                                   <  1:173221/228‑1 (MQ=255)
                                                                                                                                                                gCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTAACGTTTTAACGCCCGCAAATCCACCGGAACGAACCCCGCTCCTTTCCATTATCCGAATCCGTAATTGGCAACAAAGGGACCGCCATGACCGAAACAAGTAATAGCCCAGTGTCACCTCAACCGGAAGAAGAATATCGCGTCCAGGCCTCAtaca                                                            >  2:207708/1‑226 (MQ=255)
                                                                                                                                                                 cGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCAtt                                                              <  1:455766/227‑1 (MQ=255)
                                                                                                                                                                      tAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGCCTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGAGGAACATCGCGTCAGGCCCCCCATACGTTCa                                                       >  2:6429/1‑229 (MQ=255)
                                                                                                                                                                      tAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAg                                                                                                                        <  2:229117/164‑1 (MQ=255)
                                                                                                                                                                      tAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAg                                                                                                                        >  1:229117/1‑164 (MQ=255)
                                                                                                                                                                         cGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTACCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCAACCTAAACCGGACGATGAATCTCTGGTCAGTGCCTCATTACGTTCAGc                                                     >  1:664642/1‑228 (MQ=255)
                                                                                                                                                                              cGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCCGCATCTGAACTGGTCGACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGAAGATACTCCCGTCATTGACCCATAACATTCAGaaaacca                                               >  2:94518/1‑225 (MQ=255)
                                                                                                                                                                                    tttgtAATTTTCGAACTCCCGGAACCTGGAGCGCGAAAACCAATTCCACTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATTGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATc                                         <  2:318120/227‑1 (MQ=255)
                                                                                                                                                                                     tgttaattatCGAACGCCCGGAACCTGGACCGGGAAAACCAATTACCCTTTTTTACCGTTTGGATGGTCGCAGAGCAACTGGAACGACGCCCGCTAATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATc                                         <  2:208837/219‑1 (MQ=255)
                                                                                                                                                                                      tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCCTTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGGGCCTCATTACGTTCAGCACTTCAAAAATCCg                                       >  1:73454/1‑229 (MQ=255)
                                                                                                                                                                                      tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGCATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGCTGAATCTCGCGTCAGTGCCTCTTTCCGTTCAGCAATTCAAAAAt                                          >  1:241441/1‑226 (MQ=255)
                                                                                                                                                                                      tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAAtct                                                                               >  2:361989/1‑189 (MQ=255)
                                                                                                                                                                                      tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAAtct                                                                               <  1:361989/189‑1 (MQ=255)
                                                                                                                                                                                      tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATcc                                        >  1:32455/1‑228 (MQ=255)
                                                                                                                                                                                      tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCCCGTCAGTGCCCCATTACGTCCAGCAATTAAAAAATACg                                       >  2:581681/1‑229 (MQ=255)
                                                                                                                                                                                      tgtAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGATGCACCCTCAACCGGAAGATGCATCTCGCGTCAGTGCCTCATTACGTTCAGCACTTCCAAAAACCg                                       >  1:294250/1‑229 (MQ=255)
                                                                                                                                                                                       gtAATAATCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCgg                                      <  1:656304/229‑1 (MQ=255)
                                                                                                                                                                                         tattttCGAACTTCCGGGAGCGGGAGCCGGAAAACCAATTCCCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCa                                    <  2:528711/225‑1 (MQ=255)
                                                                                                                                                                                         tatatTCGAACTTCCGGAACCTTGTGCGCGAAAACCAATTCCCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCa                                    <  2:131355/228‑1 (MQ=255)
                                                                                                                                                                                         aaTTTTTGAACTTCCGTAACCTGGAGCGTCAAAACCAATTCCCCTTTTTTACCGTTTGACCGCTCGCAAATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCa                                    <  2:131097/229‑1 (MQ=255)
                                                                                                                                                                                             tttgattttCCGGAACCTGGGGGCCGAAAACCAATTCCCCTTTTTTACCCTTTGACTGCTCGCAGATCACCGGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATgg                                 <  2:347194/221‑1 (MQ=255)
                                                                                                                                                                                                gAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGACGCAGTTAATCGCCATCTGCACCCTAAACCGGAAGATGAATCTCCCGCCAGTGCCTCCTTACTCTCAGCAATTCAAAAATCCGCCATgggttt                             >  2:370766/1‑225 (MQ=255)
                                                                                                                                                                                                gAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGGAtt                             >  1:16633/1‑229 (MQ=255)
                                                                                                                                                                                                gAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGCCATGGTAtt                             >  1:279787/1‑229 (MQ=255)
                                                                                                                                                                                                              tGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTg               >  1:471776/1‑229 (MQ=255)
                                                                                                                                                                                                               ggggccccaaaaCCCAATCCCCCTTTTTCCCGTTAGACTGCTCGCAGATCATCTGGAACGGCGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTgg              <  1:207708/221‑1 (MQ=255)
                                                                                                                                                                                                               ggAGCGCGAAAACCAATTCCCCTTTTTTACCGTTTGACTGCTAGCAGATCCTCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTgg              <  1:431482/229‑1 (MQ=255)
                                                                                                                                                                                                               ggAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTgg              <  1:124905/229‑1 (MQ=255)
                                                                                                                                                                                                               ggAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATATCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGAATGGAATTGCTGATTGATTTTg               >  1:179977/1‑228 (MQ=255)
                                                                                                                                                                                                                    cctaaaaCCAATTCCCCTTTTTTACCGTTTGACTGCGCGCAGATCATCTGGAACGCCGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACa         <  2:279787/226‑1 (MQ=255)
                                                                                                                                                                                                                     ctaaaaCCCAGTCCCCTTTTTTCCCGGTTGAATGGTAGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACa         <  2:649801/226‑1 (MQ=255)
                                                                                                                                                                                                                     cggaaaCCAATTCCCCTTTTTTACCGTTGGGCTGCTCGGAGATCATCTGGAACGGCGCCCGCTCATTGCCAATACCAGAATCGGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACa         <  2:32455/225‑1 (MQ=255)
                                                                                                                                                                                                                     cgAAAACCAATTCCCCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACa         <  1:94518/228‑1 (MQ=255)
                                                                                                                                                                                                                     cgAAAACCAATTCCCCTTTTTTACCGTTTGACTGCTCGCAGATCAGCTTGAACACCGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACa         <  2:403786/228‑1 (MQ=255)
                                                                                                                                                                                                                     cgAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGTACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACa         <  1:155927/228‑1 (MQ=255)
                                                                                                                                                                                                                     cgAAAACCAATTCACCTTTTGTACCGGTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACa         <  1:530037/228‑1 (MQ=255)
                                                                                                                                                                                                                     cgAAAACCAATTCACCTGTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTTATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACa         <  1:696802/228‑1 (MQ=255)
                                                                                                                                                                                                                     ccaaacCCAATTCCCCTTTTTGCCCGTTTGACTGCTCCCTGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTGCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACa         <  2:294250/226‑1 (MQ=255)
                                                                                                                                                                                                                     caacaaCCAATTCACCTTTTTTACCGTTTGAACGCTCGCAGATCAGCTGGAAAGAGGCCCGCTCATTGCCATTACCAGAATCGGAACTGGTCAACAAAACGACGGAATTGACTGAAGCGGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGGATCTCGGGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACa         <  2:155094/224‑1 (MQ=255)
                                                                                                                                                                                                                     agAAAACCAATTCCCCTTTTTACCCGTTTGACTGATCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAGATCCGGCATGGTATTGCTTGATGATTTTGGCGACa         <  1:725545/227‑1 (MQ=255)
                                                                                                                                                                                                                            cAATTACCCTTTTTTACCGTTTGACTGCCCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATGACCAGAAACCGAACTGGTCAACAAACCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAAGCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACt  <  2:36634/228‑1 (MQ=255)
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                             
TCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAACTGGTCAACAAAGCGACGGCATTGACTGAAGCAGTTAATCGCCAGCTGCACCCTAAACCGGAAGATGAATCTCGCGTCAGTGCCTCATTACGTTCAGCAATTCAAAAATCCGGCATGGTATTGCTTGATGATTTTGGCGACATTGTACT  >  NC_000913/3527496‑3527943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: