Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,665,698 T→C 24.0% D38G (GAT→GGT)  gadX ← acid resistance regulon transcriptional activator; autoactivator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,665,6980TC24.0% 77.5 / 9.1 49D38G (GAT→GGT) gadXacid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base T (33/4);  new base C (2/10);  total (35/14)
Fisher's exact test for biased strand distribution p-value = 6.59e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAC  >  NC_000913/3665470‑3665833
                                                                                                                                                                                                                                            |                                                                                                                                        
ttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGCAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGCTTTAATGCCTCCTCCTTGAGAATCGGGCGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCACAAAACTTATCTACTCGAATTTGGCTTTCAt                                                                                                                                          >  2:506942/1‑229 (MQ=255)
ttACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCAt                                                                                                                                          <  1:52275/229‑1 (MQ=255)
      aGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGCATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCgag                                                                                                                                    >  1:727044/1‑226 (MQ=255)
      aGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTAACATACTCGAATTTGGCTTGAATCCGCa                                                                                                                                    >  2:710204/1‑228 (MQ=255)
      aGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAAAACACTTATCTACTCGAATTTGGCTTGCATCCGCac                                                                                                                                   >  1:464856/1‑228 (MQ=255)
        cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCTTGTCCCTTGCCACGAATACAAAATTTTCAAAAAACTACTCACTTCGAATTTGGCTTGCATCCCCaaa                                                                                                                                  >  2:23026/1‑228 (MQ=255)
        cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTTTCCCTTGACACGAATACAAAATTTTCAACAAACTTAACAACTCAAATTTGGCTTGCACCCGAaaaa                                                                                                                                 >  2:350564/1‑229 (MQ=255)
        cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaa                                                                                                                                  >  1:100113/1‑228 (MQ=255)
        cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaa                                                                                                                                  >  1:714788/1‑228 (MQ=255)
        cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTAAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaa                                                                                                                                  >  2:141627/1‑228 (MQ=255)
        cATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTACTTGAGCATCGGGAGACATACTTAAAGCTGGTCCCTTGACACGAATACAAAATTTTCAAAACACTTATCTACTCGAATTTGGCTTGAATCCGCaaa                                                                                                                                  >  2:476190/1‑228 (MQ=255)
                     cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGACGATTTAATGCATCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCACACGCAAAAACCAGGTCACCGc                                                                                                                    >  1:62175/1‑229 (MQ=255)
                     cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACCCGCATACAAAATTTTCAAAAAACTTATCTACTCGAATTTGGCTTGCATCCCAAAAAAcaaaataaacac                                                                                                                    >  2:84597/1‑218 (MQ=255)
                     cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATCCAAAATTTTCAACACACTTATCTACTCGAATTTGACTTGCATCCGCAAAAACAAGGTCACCGc                                                                                                                    >  1:706218/1‑229 (MQ=255)
                     cGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGACCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCCATTTTGGCTTGCATCCTCAAAAACCAGGACACCGc                                                                                                                    >  1:348372/1‑229 (MQ=255)
                          aTTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACAAACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCCCCATTa                                                                                                               >  2:721307/1‑229 (MQ=255)
                                aTTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCCCAATTAAAAAAg                                                                                                         >  2:299879/1‑229 (MQ=255)
                                      cGATCGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGTAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATACCGatat                                                                                                   >  1:242491/1‑229 (MQ=255)
                                      cGATAGTTGCGCAACTTCCTGATAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACCCGAATACAAAATTTTCAACACACTTATATACCCGAATTTGGTCTCATTCCGCAAAAACCAGGTCACCACCATTAAAAAAGcaaaa                                                                                                    >  2:340144/1‑224 (MQ=255)
                                      cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCAt                                                                                                                                          >  1:42009/1‑191 (MQ=255)
                                      cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGata                                                                                                    >  1:282403/1‑228 (MQ=255)
                                      cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTACCCCCCCTTAAAAAAGCggaat                                                                                                   >  2:307521/1‑225 (MQ=255)
                                      cGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTAACCGCCATTAAAATAACGata                                                                                                    >  2:474987/1‑228 (MQ=255)
                                      agATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCAt                                                                                                                                          <  2:42009/190‑1 (MQ=255)
                                       aaTAGTGGCGAAATTTCCTGAAAAACAGATATTTCTCTACTAAAGGGAAGAAGGGGTAATGAAGAAACTTTTTTATGGGCATGATGTTTAAATGCTTCCCCCTTAAGCTTAGGGAAAAATAATGAAAGCGTGCCCCTTGACTCAAATACAAAATTAACACCACCCTTCTCTCCTCAAATTTGGCTTGCCCCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATAtt                                                                                                  <  1:466076/228‑1 (MQ=255)
                                                     ctcacaaaaaaCAGGAAATCTCTTTACTACATGTATGAACGAGTAAGGAAAAACTTTTTTTAGGGGAATAAAGATTTAATCCCTCCCCCTTGACAATGGGGAAAAATAAGAAAAGCTTGCCCCTTGACACAAATAAAAATTTTTCAACCCTTTTATCTACTCAAATTTGGCTGGCACCCAAAAAAACCAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCAt                                                                                     <  1:721308/223‑1 (MQ=255)
                                                       cataaaaaaaAGGAATACCTCTAATACAGTGAGGAACAAAAAAGGAAAAAATTTTTTAATGTGCATGAAGATTGAATCCCTCCCCCTTAAGCACCGGGAAAAAAAATGAAGGCGTGTCCCTTGACACAAAAAAAAATTTTCAAACACATTTATCTACTCAATTTGGGCTTCCACCCAAAAAAACAAGTTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                   <  1:368925/224‑1 (MQ=255)
                                                                   gAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGAATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGGGAGAATAAAttt                                                                      >  1:541373/1‑229 (MQ=255)
                                                                        ttctaTACTACAGGGATAAACGAGTAATAAAAAATTTTTTTTATGGGAAGAAAGTTTGAATGCCCCCCCCTTGGCCATCGAGAAAAAAAATAAAGGGTTGTCCCTTGAACCAAATAAAAAATTTTCACCACTCTTATCTCCTCGATTTTGGTTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                  <  1:299879/228‑1 (MQ=255)
                                                                               ctacAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCAt                                                           >  1:379602/1‑229 (MQ=255)
                                                                                  cAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCCCCATTAACCATGGGGAGAAAATATATAAGTCTTGCaaaac                                                        >  2:715340/1‑225 (MQ=255)
                                                                                     tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAAc                                                                                                                               >  1:317847/1‑155 (MQ=255)
                                                                                     tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATAtt                                                                                                  >  1:288907/1‑184 (MQ=255)
                                                                                     tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCa                                                      >  1:214380/1‑228 (MQ=255)
                                                                                     tgatgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAAAAGCGATATTCACCATTAACCATGGTGAAAATAAATTTATTTTTTGCAAACGCa                                                      >  2:709772/1‑228 (MQ=255)
                                                                                       atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAg                                                                                                         >  1:429384/1‑175 (MQ=255)
                                                                                       atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTAGGAATATATTAATGTCTTGCATACGCAAt                                                    >  1:102645/1‑228 (MQ=255)
                                                                                       atgaACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGAGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTACCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTATTGCAAACGCACtt                                                   >  2:379579/1‑229 (MQ=255)
                                                                                            cgggTAAAGAAAAAACTTTTTTATTTGACAGAAAGTTTTATGGCCTCCCCATTAAACATCGGAAAAAATAATGAAAGCGTGTCCCTTGACACAAAAACAAAATTTTCACCCCATTTATCTACTCAAATTGGGCTTGCATCACAAAAACCCGGGTACCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                               <  1:709773/226‑1 (MQ=255)
                                                                                             gaaaaaagaaaaaATTTTTTTATGTGCAAAAAGATTTAACGCCCCCTCTTGGAGACGCGGAAAAAATAATGAAGGGGTGCCCCTTGACACAAATACAAAATTTACAACACTTTTATCTACTAGATTTTGGCTTCCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                             <  1:487594/222‑1 (MQ=255)
                                                                                              aattaTGAAAAAAATTTTTTCATGGGAATGAAGTTTGAATCCCCCCTGCTGGAACATCGGGAAAAAAAATGAAAGCTTCTCCTTAAACACGAATACAAAATTTACAACACTATTATTACCTGGAATTGGGTTTGCACCCAAAAAACCCAGATATCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                             <  1:340144/225‑1 (MQ=255)
                                                                                               gTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCACGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATCTATTTATGTTTTACATAACCAATTAAAAAAtt                                           >  2:76146/1‑229 (MQ=255)
                                                                                                taataaAAAATCTTTTTTATGTGAATAAATATTTAACCCCCCCCTCTAGACCATCGGAAAAAATAATGAAAGGGTTCCCCTAGACACGAATAAAAAATTTCCAACCCACTTATCTACTCAAATTTGGTTTGCATCCAAAAAACCCAGTTCTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                             <  1:350564/226‑1 (MQ=255)
                                                                                                      aaaactttttttAGGTGCATGAAGTTTTAATCCCCCCTCATTGAACATAGGGAAAAAAAATGAAGGCGTGTCCCTTAAAAAGAATACAAAATTTTCAACACACTTATCACCGAGAATTTGGTTTCATCCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                    <  1:636742/223‑1 (MQ=255)
                                                                                                               tttATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCAGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATACATTTATGTCTTGCATACTCAATTAGACAATTCCCATGTAGAGATTga                           >  2:98701/1‑228 (MQ=255)
                                                                                                                     gtgAATGAACTTTTAATGCCCCCCCTTGGAGCAGGGGAAAAAATAATGAAGGGGTGCCCGTTAACACGAATACAAATTTTCCAACCCATTTATCTACTAAAATTTGGTTTGCACCCAAAAAACCCAGCTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                      <  1:98701/229‑1 (MQ=255)
                                                                                                                       tgtaaaaataTTTTATGCCCCCACCCTGAAGAATCGGGAGAAAAAAAGAACGGGTGCCCCTGACAAGAAAAAAAAATTTCACAACCATCTTCTAACCATGAGTGTGGTTGCCACCCACAAAAACGTGTACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                      <  2:167328/220‑1 (MQ=255)
                                                                                                                       gCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                    >  1:555811/1‑229 (MQ=255)
                                                                                                                                      tGCCTCCTCGTTGAGCAGGGGGAAAAAAAAGGAGAGGTTGCCCGTTCACAAAAATAAAATTTTTCCACAACAATTATTAACACAAATGGGGGTTGAACCCAAAAAAACAAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt      <  1:74296/229‑1 (MQ=255)
                                                                                                                                      tGCCTCCTCCTTGAGAGTGGGAAAAAATAAAGAAAGCTGCTCCCGTGACAGAAAAAAAAATTTTCAAAACCTCTTACCTCCTAGATTTTGGTTTCAATCCGAAAAAACAAGGTCGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt      <  1:76146/229‑1 (MQ=255)
                                                                                                                                      tGCCTCCCCCTAGAACATCGGGAAAAATAAAGAAGCCTTGCCCTGTAAAAACAATAAAAAATTTACACCACTCTTATCAACTAAATTTTGGCTTGCACCCGAAAAACCGGGGACACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt      <  1:224154/229‑1 (MQ=255)
                                                                                                                                          tcctcctTGAGCATTGGGAGAAATAATGAAAGAGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGTTATTCACCATTAACCATGGTGAGAATATATTTATGGCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCCTAGTTGACTTAAAATTACATaaca  >  1:160036/1‑227 (MQ=255)
                                                                                                                                                         cGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                       >  2:408626/1‑130 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                        
TTACGTAGCATTTCTGCATAGCGAAGATTCTTATTCTGCGATAGTTGCGCAACTTCCTGAAAAACAGGAATATCTCTACTACAGTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAC  >  NC_000913/3665470‑3665833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: