Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,241,829	T→A	12.7%	L142F (TTA→TTT)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,829	0	T	A	12.7%	161.1 / 5.6	72	L142F (TTA→TTT)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand):  ref base T (46/15);  new base A (2/8);  total (49/23)
Fisher's exact test for biased strand distribution p-value = 1.29e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

ATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCC  >  NC_000913/4241601‑4242044                                                                                                                                                                                                                                                |                                                                                                                                                                                                                        ataCAGGAGCATTAAGAACAGCAGTGCAGGGATGCATTCCGAGGCAAACATATAACGCCAGCCGGCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCt                                                                                                                                                                                                                         <  1:282516/229‑1 (MQ=255)      ccagcaTTAAAAACGGAATGGAAGGGAACAATTCCGAGGCAAACGTTAAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCCCCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAAcc                                                                                                                                                                                                                    <  2:574163/228‑1 (MQ=255)          caTTACGAACAGCAGTGCAGGGAAACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCAAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑A‑GCGAATATGAGCTGGAGCCAGTTC‑TG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACg                                                                                                                                                                                                                >  1:630980/1‑229 (MQ=255)          caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTT‑CG‑CAAT‑ATACA‑TTGACGAGAGCATTGAGGCTAAACCaag                                                                                                                                                                                                                 >  1:734237/1‑227 (MQ=255)          caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACg                                                                                                                                                                                                                >  1:228825/1‑229 (MQ=255)          caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAAGGCATTGAGGCAAAACCaaac                                                                                                                                                                                                                >  2:705690/1‑227 (MQ=255)          caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATAGAAGCTGGGGCCAGTTC‑CC‑CAAT‑AAAAA‑TTGGCGAGGGCATTTGGCCAAAACCaaac                                                                                                                                                                                                                >  2:187193/1‑227 (MQ=255)          caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACC‑GAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑CCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGCGCAAAGCATTGAGGCTAACCCAACGc                                                                                                                                                                                                               >  1:687328/1‑229 (MQ=255)                    agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGc                                                                                                                                                                                                      >  1:215237/1‑229 (MQ=255)                      cagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGcaa                                                                                                                                                                                                    >  1:687725/1‑227 (MQ=255)                      cagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGAAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAAAATGAGCTGGAGCAAGTTC‑AG‑CAAT‑AAACA‑TTGGCGAGAGCATTTGGGCGAAACCAAAGcccaacccgcaa                                                                                                                                                                                                    >  2:708797/1‑219 (MQ=255)                         tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCaata                                                                                                                                                                                                 >  1:114793/1‑229 (MQ=255)                         tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCaata                                                                                                                                                                                                 >  1:405776/1‑229 (MQ=255)                                aTACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTA‑CG‑CAAT‑ATACA‑TTGGCGAGAGAATTGAGGCTAAACCAACGCCAATACCTCAAATAATGGGat                                                                                                                                                                                          >  1:688862/1‑229 (MQ=255)                                   catccggagcaaaaCATAAACCGCCAGCCGTCAGTATTCAGCCAGCTGGATACACCGGAACGGGA‑AATAAAAGA‑GTTTACGCAGTAAACTAAATGG‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACAAGTTTCC‑C‑GCGAATATGAGCGGGATCCAGTCC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCAAAACCAACGCCAATACCGCCAATAATGCGataa                                                                                                                                                                                        <  1:256371/217‑1 (MQ=255)                                   cATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataa                                                                                                                                                                                        >  1:193971/1‑228 (MQ=255)                                         gAGGCAAAAATATAACGCCGGCCACCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AAAAAAATA‑GTTTACGCAGTAAACAAAAAGT‑T‑CCCCGAAAATAACCGAAAACTGGTTAAAA‑GAGACCAGTTCCC‑C‑GGGAATATGAGCTGGAGCCAGTCC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAACCCAACGCCAATACCGCCAATAATGCGATAAATAACa                                                                                                                                                                                  <  1:187193/228‑1 (MQ=255)                                          aGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGATAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCAAGTTC‑GG‑CAAT‑ATACA‑TTGTCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATTCGATAAAAAACaaa                                                                                                                                                                                >  2:223130/1‑229 (MQ=255)                                                aCATATAACGCCAGCCGTCCGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑AAGACCAGTTTCC‑C‑GCGAATATCAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGCGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATCTGATAAATAACAAATTcccg                                                                                                                                                                          >  1:543977/1‑227 (MQ=255)                                                  ataaagccacAGCCTTCAGTATAGTGCCAGCGGGCATCACCGGAGTGTGC‑AATAAATTA‑TTTTCCGCAGAAAATTAAAAGT‑T‑CCCCAAAAATATTCAAAAGCTGGTTAAAA‑GAGCCCATTTTCC‑G‑GGAAATATGAGCTGGAGCCGGTTC‑CC‑CAAT‑ATACA‑TTGGAGAGAGCATTGAGGCTAACCCACCGCCAAAACCGCCAATAATGCGATAAATAACAAATTCCGGGa                                                                                                                                                                        <  1:607390/220‑1 (MQ=255)                                                      aaCGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑ATTTACGCAGTAAACTAAAAGT‑T‑GCCCGCAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTAC‑C‑GAGAATATAAGCTGGAGCCAGTTC‑CG‑AGAT‑ATACA‑TGTCCGAGAGCATGTAGACTAAAGCAATGCCAATCACTCCAATAATGAGATAATTAAACAATACCGGGACATa                                                                                                                                                                    >  1:348797/1‑229 (MQ=255)                                                        ccgcAGCCGTTAATATCCAGCCATCGGGAATCACCGGAAGGAGA‑AAAAAAATA‑GTTTACGAAGAAAAC‑AAAAGT‑TGGCCAAAAAATAACCGCACACGGGTTAAAA‑GCGACCATTTTCC‑A‑GAAAATATGACCTGGACCCATTCC‑CG‑AAAT‑TTACA‑TTGGCGAGAAAATTGAGGGAAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAAc                                                                                                                                                                  <  1:705691/226‑1 (MQ=255)                                                          ccAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTCAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TCGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCCATAATGCGATAAATAACAAATTCCGGGaataaaccc                                                                                                                                                                >  2:164663/1‑221 (MQ=255)                                                           cAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAAGAATGCGATCAATAACAAATTCCGGGACATAACCTg                                                                                                                                                               >  1:264231/1‑229 (MQ=255)                                                            aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGc                                                                                                                                                              >  1:483893/1‑229 (MQ=255)                                                            aGCCGTCAGTATTCAGCCAGCTGCCATCACCGGCACGGGC‑AATAAAATA‑GCTTACGCAGTAAACTAAAAGC‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGGTAAATAACAAATTCCGGGCCATAACCTGc                                                                                                                                                              >  1:248309/1‑229 (MQ=255)                                                             gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGGGCATTGAGGCTAAACAAACGCCAATACCGCCAATAATGCGATAAATAAAAAATTCCGGAAAAAAACCTGcc                                                                                                                                                             >  2:271024/1‑229 (MQ=255)                                                                    gTATTCAGCAAGTTGGCATACCCGGAACGGGA‑AAAAAAATA‑GTTTACGAAGTAAACTAAAAGT‑C‑GCCCAAAAATAATCGAAAACTGTTTAAAA‑GAGCCCAGTTCCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCACCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATaaa                                                                                                                                                      <  1:88385/229‑1 (MQ=255)                                                                    atataCAACCAGCGGGCAACACGGAAACGGAA‑AAAAAAATA‑GTTAACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAACCGAAAACTGGTAAAAA‑GACACCATTTCCC‑G‑GCAAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑AAACA‑TTGGCGAGAGCATTGAGGCAAAACCACCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATaaa                                                                                                                                                      <  1:205390/228‑1 (MQ=255)                                                                     tataCAGCACGCTGGAATCACCGAAACGGGC‑AATAAAATA‑GTAACCGCAATAAAC‑AAAAGTGT‑GCCGGAAAAAAGTCCAAACCTGGTAAAAA‑AGCACAAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑AAAT‑AAACA‑TGGGCGAGAGCATTGAGGCTAAACCAACGACAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATaaa                                                                                                                                                      <  1:204351/228‑1 (MQ=255)                                                                     tagccacccagcTGGCAGGCCCGGAA‑GGGC‑AATAAAAGATTTTTACGGATTAAACGTAATTT‑T‑GTCGGAAAATAATTGTAAATTGTTAAAAA‑GAGCCTAGGTACC‑G‑GAGGAGTGGAGCGGGAGCCATTTC‑CG‑CCAT‑ATATA‑TTGCTGAGACACTGGAGGCTAAACCAGCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGTGACCTAACCTGCCAGATAAAc                                                                                                                                                     <  1:592414/222‑1 (MQ=255)                                                                     tATGCAGCCAGCTGGCAACACCGGAACGGGA‑AAAAAAAGA‑GTTTACACAGAAAACTAAAATT‑T‑CCCGAAAAATAATCGAAAACTGGTAAAAA‑GAGCCAAGTTACC‑C‑GCGAATATGGGCTGGAGCAAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAAc                                                                                                                                                     <  1:56329/229‑1 (MQ=255)                                                                            ccagcTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CC‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAAAACCCCCAATAATCCGATAAATAAAAAATTCCGGGACATAACCTGCCCAATAAAAAGGcaca                                                                                                                                              >  2:161033/1‑229 (MQ=255)                                                                               gcTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAgtg                                                                                                                                           >  1:189920/1‑229 (MQ=255)                                                                               gcTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTT‑C‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAAACTGCCAGATAAACAGGcacaaggt                                                                                                                                          >  1:646341/1‑225 (MQ=255)                                                                                 ggggcaccacGGAAAGGGCAAAAAAAATA‑GTTTACGCAGAAAACAAAAATT‑G‑GCCGGAAAATATTC‑CAAACCGGTTAAAAGGAGACCATTTCCG‑C‑GGAAATATGAGCTGGACCCAGTTC‑CG‑AAAT‑ATACA‑TTGACGAGAGCTTTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAg                                                                                                                                             <  1:153368/217‑1 (MQ=255)                                                                                    cATCACCGGAACGGGC‑AATAAAATA‑TTGTCCGCAGTAAACAAAAGGT‑T‑GCCCAAAAATACGCGAAAACTGGGTAAAA‑GAGCCCATTTTCC‑C‑GATAATATGAGCTGGACCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATGGGGGCTAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                                      <  1:708798/229‑1 (MQ=255)                                                                                       cccggaaaCGGAA‑AAAAAAAAG‑GTTTACGAAGAAAACAAAAAGT‑T‑CCCGAAAAATAATCGCAACCTGGTAAAAA‑AAGACCAGTTTCC‑C‑GAGAATATGAGCTGAGCCCAGTCC‑CG‑AAAT‑ATCCA‑TTGGCGAGAGCATTGAGGCTAAACCAAGGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCgg                                                                                                                                   <  1:271024/223‑1 (MQ=255)                                                                                                 ggC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCACAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ACACA‑TTGACGAGAGCATTGCGGCTAAACCAACGCCAATACCGCCACTAATGCGATAAATAACAAATTTCGGGACCTAACCTGCCAGATAAACAGGCACCGTGTTCTACTGGTTTATagag                                                                                                                         >  1:644739/1‑229 (MQ=255)                                                                                                  gC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCACTAATGCGCTAAATCACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAAGGTTGTCCGGAGTTATAAAgg                                                                                                                        >  1:286308/1‑229 (MQ=255)                                                                                                  gC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCAAAGTGTTGTCCGGGTTtataaatg                                                                                                                        >  1:237876/1‑225 (MQ=255)                                                                                                       tAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATACAAAATTCCGGGCCAAAACATGCCCAGTAACCAGGCACAGTGTTATCCGGGttttaataggt                                                                                                                       >  1:355864/1‑218 (MQ=255)                                                                                                        aaaaTT‑GTTTACGTATAAAAATAAAAGT‑T‑GGCAGAAAAAAAGCAAAACGGGGATAAAA‑AAGACCATTTTCA‑C‑CAAAAAAGGGGCAGGAGCC‑GTTC‑CGACAAT‑AGAAT‑TGGGCGACGACTTTGAGGAAAACCAAACACAAAAACCGCCAAGAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCTCAGTGTTGTCCGGGTTTATAGAGGTa                                                                                                                      <  2:543977/226‑1 (MQ=255)                                                                                                                  tACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTTAGGCTAAACCAACGCCAATACCGCCAATAATCCGATAAATAACAAATTCCGGGACATAACCTGCCAACTAAACAGGCCCAGGATTTTCCGGGTTTTAAAAGGTAAAACCaaaatta                                                                                                          >  2:64823/1‑224 (MQ=255)                                                                                                                  tACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGAGTTGTCCGGGTTTATAGAGGTAAAACCACGTTCt                                                                                                          >  1:538059/1‑229 (MQ=255)                                                                                                                    cGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTg                                                                                                         >  1:598650/1‑228 (MQ=255)                                                                                                                         tAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCGGCCAGATAAACAGGAACAGTGTTTTACGGGTTTAAAAAGGTAAAACAAAATTCAGGCCAgg                                                                                                   >  2:287611/1‑229 (MQ=255)                                                                                                                            acaaaaaGT‑T‑GCCCAAAAAAAAGACGAAATTGGCTAAAA‑AGGGCCGGGTGCCGC‑GCAAAT‑TGAACTGGACCCAGTTCTCG‑AATG‑AAACG‑TGGGGGAGAGCATTGAGGCAAACCCATCGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTGCTGGCCAGGCa                                                                                                 <  2:259638/226‑1 (MQ=255)                                                                                                                             tgaaaaGC‑T‑GCTCGAAAAAAATCACAAACTGGTTAAAA‑CAGACAAGTTCCC‑C‑GGAAATTGGAGTTGGAGCAATTCC‑CA‑CAAA‑AACCA‑TTGGCGAGAGCATTGAGGTTAAACCAACGCCAAGACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGa                                                                                               <  1:401305/227‑1 (MQ=255)                                                                                                                                 aaGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGata                                                                                                                                                                                         >  2:162967/1‑135 (MQ=255)                                                                                                                                 aaGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TGGGCGAGAGCATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGata                                                                                                                                                                                         <  1:162967/135‑1 (MQ=255)                                                                                                                                        gCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGCCACAGTGTTGTCCGGGTTTATAGAGTAAAAACCAAGTTCTGGCCAGGCAGAACCTACAcc                                                                                      >  1:558234/1‑229 (MQ=255)                                                                                                                                         cccGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGATCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGACCAGTGTTGTCCGGGTTTATAGAGGGAAAACCAAGTTCTGGCCAGGCAGAACCTAAAccc                                                                                     >  1:690006/1‑228 (MQ=255)                                                                                                                                         cccGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCAAAGTTTTGTCCGGGGTTATAGAGGTAAAACAAAGTTCTGGCCAGGCAGAACCAACACCa                                                                                     >  2:607011/1‑229 (MQ=255)                                                                                                                                                    gcgtatggtgtttAAAA‑GAG‑CATGTTTCC‑CGGAGAATTTGCGCTGGAGCCAG‑TC‑CG‑CAATGATCCA‑TGGGGGAGAGCATTGAGGCAAACCCAACGCCAAACCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaaaa                                                                          <  2:105101/218‑1 (MQ=255)                                                                                                                                                                 taaaaaaacccAGTTCCC‑C‑GGAAATATGGACTGGACCCAGTCC‑AG‑AAAT‑ATCCA‑TTGGCGAAAGCATTAGGGCAAAACCAAGGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACagcagc                                                              <  1:287611/220‑1 (MQ=255)                                                                                                                                                                       agaCCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACAAACAAGAAAAAACCGGAcagaagaaccct                                                         >  2:137517/1‑222 (MQ=255)                                                                                                                                                                         aCCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGATGTCCGGGTTTATAGCGGTAAAACCAAGTTCTGGCAAGGCAGAACCTACACCAGAAATAAAAAAAAGGAAAGCCGCAATCTaaa                                                      >  2:227196/1‑229 (MQ=255)                                                                                                                                                                         aCCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCAAAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCGGAACTAAAAAACAGGACAGCAGCAATCTTaa                                                      >  1:171365/1‑229 (MQ=255)                                                                                                                                                                                          atatGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATaaaaaa                                                                          >  1:105101/1‑194 (MQ=255)                                                                                                                                                                                                     gaccAGTCC‑CG‑AAAT‑ATACA‑TGGGCGAAAGAATGGGGGCAAACCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTa                            <  1:424849/227‑1 (MQ=255)                                                                                                                                                                                                     acccAGTTC‑C‑‑CAAA‑TTACAGTTGGGAAGAGAATTGAGGCTAACCCAACGCCAATTCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTa                            <  1:161033/227‑1 (MQ=255)                                                                                                                                                                                                           ttC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACCGCACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGt                              >  1:376221/1‑221 (MQ=255)                                                                                                                                                                                                                        taCA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTTCAATAACCCCCGa            >  1:374412/1‑229 (MQ=255)                                                                                                                                                                                                                           a‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGCTAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGCAAGCAGCAATCTTAAGTGAATCACGACGACCCAAGCGGTTACTGCAAAAAACACCGAggg         >  2:550227/1‑229 (MQ=255)                                                                                                                                                                                                                                 cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACAACCGAGGGCAccc    >  1:653485/1‑228 (MQ=255)                                                                                                                                                                                                                                 cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTAACAGCAAAAAACACCGAGGGCAccc    >  2:29514/1‑228 (MQ=255)                                                                                                                                                                                                                                   agagCATTGAGGCTAAACCAACGCCAATACCTCCACTAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATTAAAAACAGGAAAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAAACACCGCGGGCAccgc   >  1:651468/1‑228 (MQ=255)                                                                                                                                                                                                                                   agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGCTAAATAACAAATTCCGGGACATAACCTGCCAGAACAACGGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGAAGAACCTACACCAGAAATAAAAAACAAGACAGCAGCAATCATAAGGAAATCACAACAAACGAAACGGGTAATGCAGAACACACCAAGGGCaaaacc  >  2:693212/1‑224 (MQ=255)                                                                                                                                                                                                                                   agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                                      >  1:657511/1‑97 (MQ=255)                                                                                                                                                                                                                                   agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                                      <  2:657510/97‑1 (MQ=255)                                                                                                                                                                                                                                   agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                                      >  1:607553/1‑97 (MQ=255)                                                                                                                                                                                                                                   agagCATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                                      <  2:607552/97‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                        ATACAGCAGCATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGC‑AATAAAATA‑GTTTACGCAGTAAACTAAAAGT‑T‑GCCCGAAAATAATCGCAAACTGGTTAAAA‑GAGACCAGTTTCC‑C‑GCGAATATGAGCTGGAGCCAGTTC‑CG‑CAAT‑ATACA‑TTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCC  >  NC_000913/4241601‑4242044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑