New junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 4516891 = | 166 (1.150) | 7 (0.050) | 7/502 | NT | 4.2% | coding (827/954 nt) | fecR | anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator |
? | NC_000913 | 4516925 = | 158 (1.130) | coding (793/954 nt) | fecR | anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator | |||||
Rejected: Frequency below/above cutoff threshold. |
ACTGCTTTAGGGACAGAATTTACCGTCCGCCAGCAGGATAATTTCACGCAGCTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCG‑ATCCCGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913/4517186‑4516891 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccgttgcGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGA > NC_000913/4516925‑4517209 cCTGATTTAGGGACAGAATTTACCGTCCGCCAGCAGGATAATTTCACGCAGCTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCAGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCG‑ATCCCG < 1:1060570/288‑1 TAGGGACAGAATTTACCGTCCGCCAGCAGGATAATTTCACGCAGCTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATCGCCCCGCTAAACCGTCACCCCCACTGCGTGCTTCCGTGCG‑CACCCGCCGTTG > 2:145609/1‑288 AGAATTTACCGTCCGCCAGCAGGATAATTTCACGCAGCTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCG‑ATCCCGCCGTTGCGGTAACG > 1:1018056/1‑289 gCCGGCCGCCGGAAGGATAAATTCACCCAGCTTGACTTGCATCAGCACGCTGTGGAATGGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCGGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCG‑ATCCCGCCGTTGCGGTAACGGGTTAGC < 1:738134/288‑1 CGCCAGCAGGATAATTTCACGCAGCTTGCCGTGCAGAAGCACGCTGTGGAAGTGCTTCTCGCCAGTGACCCCGCGCAAAAACGCATCGTGAACGCTGGTGATAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCTGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCG‑ATCCCGCCGTTGCGGTAACGGGTTAGCGTGGC < 2:961155/288‑1 GGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACCAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGGGAT‑CGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCCGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCAC > 1:864851/1‑288 GACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGGGAT‑CGCAGCGCAGCACGCCGTTGCGGTCACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCCCTGCGCCAAACTCAGAGGCGCTGAACTGCCGCCATTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTT > 2:86480/1‑288 ccAAGGACAGCCTGGGCGTCAGCGATAAACCGCTGGGTGAGGTGTGAGCCACGCTAACCCGGTACCGCAACGGCGGGAT‑CGCAGCGCAGCACGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCA < 2:864851/287‑1 GCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGGGAT‑CGCAGCGCAGCACGCCGTGGCGGTAACGGGTTAGCGTGGCTATCACCCAACCCAGAGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCACCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTTCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGCCGCGAAGCACCTCCACAGCGTGCTGTTGC > 2:1018056/1‑288 gcCCGTTGCGGTAATGGGTTAGCTTGGCTATCACCCCACCCGGCGGTTTATCGCTGAAGCTCAGGATTTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGT < 2:396491/287‑1 ctCCGTGGCGGTAACGGGTTTGCGTGGCTATAACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTTTGCTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGT < 2:77902/287‑1 CGCCGTTGCGGTAACTGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGT < 1:848585/289‑1 CGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGT < 1:323155/289‑1 CGCCGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGT < 1:984890/289‑1 CGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGT > 1:818835/1‑256 CGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGT < 2:818835/256‑1 CGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCT > 1:424397/1‑254 CGTTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCT < 2:424397/254‑1 TTGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGA < 1:532529/289‑1 gtCGGTAACGGGTGAGCGTGGCTATCACCTCAACCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGGTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGGGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGA < 2:612943/286‑1 TGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAATTTCTGTCCCTAAAGCAGTGAGCTGGCCCTGCCGGGTCAGGA > 1:396491/1‑288 TGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGA < 1:53279/288‑1 TGCGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGA < 1:458517/288‑1 ACTGCTTTAGGGACAGAATTTACCGTCCGCCAGCAGGATAATTTCACGCAGCTTGACGTGCAGCAGCACGCTGTGGAAGTGCTTCTCGCCAGTGCCCCCGCGCAAAAACGCATCGTGAACGCTGGTGAAAGCCTGCAGTTCAGCGCCTCTGAGTTTGGCGCAGTGAAACCGCTGGATGACGAGAGTACAAGCTGGACGAAGGACATCCTGAGCTTCAGCGATAAACCGCTGGGTGAGGTGATAGCCACGCTAACCCGTTACCGCAACGGCGTGCTGCGCTGCG‑ATCCCGCCGTTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < NC_000913/4517186‑4516891 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccgttgcGGTAACGGGTTAGCGTGGCTATCACCTCACCCAGCGGTTTATCGCTGAAGCTCAGGATGTCCTTCGTCCAGCTTGTACTCTCGTCATCCAGCGGTTTCACTGCGCCAAACTCAGAGGCGCTGAACTGCAGGCTTTCACCAGCGTTCACGATGCGTTTTTGCGCGGGGGCACTGGCGAGAAGCACTTCCACAGCGTGCTGCTGCACGTCAAGCTGCGTGAAATTATCCTGCTGGCGGACGGTAAATTCTGTCCCTAAAGCAGTGAGCTGGCCCTGACGGGTCAGGA > NC_000913/4516925‑4517209 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |