Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,269,956 T→C 13.8% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,269,9560TC13.8% 129.6 / 2.1 56intergenic (+354/+260)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base T (31/15);  new base C (3/5);  total (34/22)
Fisher's exact test for biased strand distribution p-value = 1.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCC  >  NC_000913/3269669‑3270237
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                         
cAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAACTCTCTTCCCCTCCAAAAATa                                                                                                                                                                                                                                                                                          >  2:865255/1‑289 (MQ=255)
                cTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCg                                                                                                                                                                                                                                                                          >  1:369750/1‑289 (MQ=255)
                                                               cTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATAAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTTACAGATAATCCGctttcttt                                                                                                                                                                                                                           >  1:44912/1‑289 (MQ=255)
                                                                  cGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaaa                                                                                                                                                                                                                                                           >  2:469075/1‑254 (MQ=255)
                                                                  cGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaaa                                                                                                                                                                                                                                                           <  1:469075/254‑1 (MQ=255)
                                                                       aTCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                                                                                                                                   <  1:865255/289‑1 (MQ=255)
                                                                       aTCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCCCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                                                                                                                                   >  1:303544/1‑289 (MQ=255)
                                                                            gCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                                                                                                                                                                                                            <  1:323883/243‑1 (MQ=255)
                                                                            gCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                                                                                                                                                                                                                                            >  2:323883/1‑243 (MQ=255)
                                                                            gCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTc                                                                                                                                                                                                              >  1:547839/1‑289 (MQ=255)
                                                                               tACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGTTAtcgtgtcgtg                                                                                                                                                                                                           >  1:704288/1‑289 (MQ=255)
                                                                               tACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTg                                                                                                                                                                                                           >  1:35502/1‑289 (MQ=255)
                                                                                 cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                                                                                                                                                        >  1:359827/1‑274 (MQ=255)
                                                                                 cGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                                                                                                                                                        <  2:359827/274‑1 (MQ=255)
                                                                                  gcccgctctGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCAGGCATGAATAAAGCGCACTCAACAATCGGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAc                                                                                                                                                                                                        <  1:814114/284‑1 (MQ=255)
                                                                                      tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAgg                                                                                                                                                                                                                                                       <  1:365163/238‑1 (MQ=255)
                                                                                      tctcTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAgg                                                                                                                                                                                                                                                       >  2:365163/1‑238 (MQ=255)
                                                                                       ctctGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtca                                                                                                                                                                                                   >  2:791852/1‑289 (MQ=255)
                                                                                         ctGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTAAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcatt                                                                                                                                                                                                 <  2:547839/289‑1 (MQ=255)
                                                                                              atatatTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAAAGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTCCTGCCCCTCCAAAAGTAAAAACCCGCCGCAGCGGGTTTTGACGAAAATCAGGTGAAACTGGCCGATACACCGGTTTGTTTTGGGTATAGTATCGCGTCCAGtcctcaattt                                                                                                                                                                                            >  2:41459/1‑281 (MQ=21)
                                                                                                    tGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTg                                                                                                                                                                                       >  1:697016/1‑288 (MQ=255)
                                                                                                           gCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTCAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACCGTCATTCATCTTTCTGACACTCCa                                                                                                                                                                               >  1:571683/1‑289 (MQ=255)
                                                                                                           gCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTCATCTATCTTTATGCCCCTCCa                                                                                                                                                                               >  1:221251/1‑289 (MQ=255)
                                                                                                           gCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTACAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCa                                                                                                                                                                               >  1:596895/1‑289 (MQ=255)
                                                                                                           gCGTGCTCTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAAAAGGTGAAACTGAACGATAAGCCGCTTTTTTTCGGGGATAGTGCTCTAGCTAGTCATTCAACCTTCTGCCCCTTCa                                                                                                                                                                               >  1:21541/1‑289 (MQ=21)
                                                                                                                           aGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACATTCATTCAACTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGACGCTTTCTTTTGGGTATAGTGTCTTTGACAGTCATTCCACTTTCCTCTCCTCCTAAAACAAAACCccgt                                                                                                                                                                >  2:430468/1‑287 (MQ=18)
                                                                                                                           aGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTAATCTTTCTGCCCCTCCAAACGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTCTTTTTGGGCAAAGCGTCCTGGCCGACGAATCAGTTTTCTTCCCCTCCAAACGAAACAACccgca                                                                                                                                                               >  2:533877/1‑288 (MQ=18)
                                                                                                                             gATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                                                                                                                                          <  1:602314/228‑1 (MQ=255)
                                                                                                                             gATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                                                                                                                                          >  2:602314/1‑228 (MQ=255)
                                                                                                                               tAAGGCGTTCACGCCGCATCCGGCATAAATAAAGCGCACGCAACAAGCTGAACCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATATGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                                                                           <  1:549233/289‑1 (MQ=255)
                                                                                                                                   gCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATGAGGTGAAACTTACCGATAAGCCGCTTTCTTTTGAGGATAGTGACTGGTTTAGTGATTCATCTTTCTGCGCATCCAAAAGCACAACTCGGCCGAAGCagg                                                                                                                                                       >  2:661494/1‑289 (MQ=18)
                                                                                                                                       tCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCGTTCTTTTGGGTATAGTGTAGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCAACAAACCGCAGAAGCGGATTTTTACGTAAAACAGGTGAAACTGACCGATAAGCAGCTTTCGTTTGGGTATAGTGTGGTGATCAGTAATTCATCTTTCTTCCCCTACAAAAGAAAAAAGCCGCCGAAGCGGGattt                                                                                                                                                   >  1:821006/1‑289 (MQ=255)
                                                                                                                                        cACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTGTTGGGGATAGTGTCGTGGACAGTCATTCATCTTTTTGCCCCTCCAAAAGTACAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTCACCGATACGCCGCTTGCTGTTGGGTATAGCGTGGTGTATAGTCATTCATCTTTCTCGCCTTCCAAACTCAAACACccgcccacgtggggtctt                                                                                                                                                  >  2:138407/1‑276 (MQ=255)
                                                                                                                                                           aTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAACGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATACGCCGGTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATGCATCTGTCTGCCCCTCCAACCGCCAAAACCCGCCGAAGCCGGTTTTTACGTCAATCAGGTCCAACt                                                                                                                               >  2:754671/1‑289 (MQ=14)
                                                                                                                                                                    aCTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                                                                                                                                   >  1:587225/1‑196 (MQ=255)
                                                                                                                                                                    aCTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                                                                                                                                   <  2:587225/196‑1 (MQ=255)
                                                                                                                                                                          caaTCTGAAACCCGCCGGAACGGTTTATTACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTGCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGct                                                                                                                <  1:449913/289‑1 (MQ=255)
                                                                                                                                                                                 caccccGCAGGAGCGGGTTCTTACGGACAACCGGAAGCACTTACCGAGACGCCGGTGTCGTGTGAGTATAGTGACGTGGACAGCCGTGTAACTGACTGACCATCCAAAAGCAAAAACCTGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAATCCGCTTTCTTTTGGGTATATTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                         <  2:303544/286‑1 (MQ=11)
                                                                                                                                                                                  aaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                        >  1:313919/1‑289 (MQ=255)
                                                                                                                                                                                         cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                  <  1:430468/288‑1 (MQ=255)
                                                                                                                                                                                         aggAGCGGTTTATTACGTACATCAGGTAAAACGTACCGATAAGCCGCTTTCATTTGAGTATAGTGTCGTGGACATTCATTCATCTTTCTACCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAGCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGtata                                                                                                  <  1:445346/287‑1 (MQ=255)
                                                                                                                                                                                                ttttATTACGTACAACTGGTATAACTGACCGAGAAGCCGGTTGCTTTTTAGTATAGTGTCGTGGAAAGTGATTCATATCTCTCCCCCTCCAGAAGCAAAAACCCGCCGAAGCGTGTTTTTACGTAGAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAACCGCTTTCTTTTGGGTATAGCGTCGTg                                                                                          <  2:369750/288‑1 (MQ=14)
                                                                                                                                                                                                  ttattaCGTATTTCAGGTAAAACTGACGGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTAATTGTTCTTACGGGACATCAAAAAGTAAAAACCCGCCGAAGCGGTTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                                                                        <  2:313919/289‑1 (MQ=14)
                                                                                                                                                                                                   tattcCGTCCATCAGGTAAAACTGCCTTATCAGCAGCTTTCTTTGTGTTACAGCGTCGTGGAGAGTCATTCATCTCTCGGCCCCTCCAGAAGTAAAAACCTACCGGACCGGTTTTTTACGTACAACAGTTGAAACTGACCTATAAGCCTCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCCCCAAAAGCATAAACCCACCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAc                                                                                       <  2:593795/289‑1 (MQ=17)
                                                                                                                                                                                                           aCATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCATTCTATTGGGTATAGCGTCTTGGACAGtcatta                                                                               >  1:593795/1‑288 (MQ=255)
                                                                                                                                                                                                               cAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAAGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCt                                                                           >  1:567763/1‑289 (MQ=255)
                                                                                                                                                                                                                 tttAAAACTTTCCGTTAAGCCGCTTTCTTTTTGCTATGTTCACCTGGACACTCAGTCGTCTGTCTCCCACTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCttt                                                                         <  2:35502/287‑1 (MQ=17)
                                                                                                                                                                                                                                                  gtttgttgtCGCGGACAGTGAGACATCTTACTGCCACTCCAAAATCTTAGACCCGCCGGACCGGGTTTTAAAGTAAAACAGGTGAAACTGACCGTTAATCCGCTTTCATTTGGGTATGGTGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                         <  2:221251/282‑1 (MQ=14)
                                                                                                                                                                                                                                                  atatgGTGTCCGGGACTGTCATTCAACTTTCTGACACTCCAAAAGTGAAAACCCTCCGAAGCCGGTTATTACGTAGAACAGGTTAAAATTACCGATAAGCCGCTGTCTTTTAGGTATGGTTTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                         <  2:704288/287‑1 (MQ=14)
                                                                                                                                                                                                                                                      aGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAACGCAAAAACCCGCCGAAGTGGGGTTTTACGTAAACCAGGTTAAACATACAGATAAGCCGCTTTCGATGGGGAATAACGTGGTGGCAAGCCATGCATCTGCCCACACCACCAAAAACAAACACCAGCCCAAGCgcg                                      >  2:562345/1‑285 (MQ=14)
                                                                                                                                                                                                                                                            gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                              >  1:747869/1‑289 (MQ=255)
                                                                                                                                                                                                                                                             tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                             >  1:633602/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                      attcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCACAAGCAAAACCCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                    >  1:161278/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                             tttCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGTTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTg             <  1:533877/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ttCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGGAAATCAGGTGAACCTGa            >  1:615404/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                        ccAAAAGGGAAAACCCGCCGAATCATGTTTTTACGTAAAACAGGTGTAACTTACCGATAGGCCACTGGCTTTTGGGTATAGTGTCGTGGACAGTCGTTCATAGTGCTGACCCACCAAAAGCAAAACCCCGCTGAAGCGGGTTTTTACGTAAACCAGGTGAGACTGACCGATAAGCCGCTTTCTTTAGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGcc  <  2:721327/289‑1 (MQ=14)
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                         
CAAAAGCAAAAACTCACTGACGCGGGTTTGAAACTGATGGCAACCGCAAAAATGCCTGATGCGCTACGCTTATCAGGCCTACGCCATCTCTGCAATATATTGAATTTGCGTGCTTTTGTAGGCAGGATAAGGCGTTCACGCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCC  >  NC_000913/3269669‑3270237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑