Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300693	1300697	5–1199	41 [0]	[0] 41	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	41 (1.040)	38/276	0.1	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

AATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATT  >  NC_000913/1299357‑1299635                                                                                                                                              |                                                                                                                                          aaTTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCACGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAACAATATTGGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAa                                                                                                                                          >  2:710180‑M1/1‑142 (MQ=255)       agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCCGGGCTTGCTGAAGAATAATTGAAAAGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataa                                                                                                                                    <  1:308767‑M1/143‑8 (MQ=255)               ataAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAGTAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcagttgt                                                                                                                            >  2:120276‑M1/1‑128 (MQ=255)                taAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTCCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                           >  1:67122‑M1/1‑127 (MQ=255)                taAGGACTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtt                                                                                                                           <  2:220864‑M1/143‑17 (MQ=255)                      tttCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCACAACATCATAATCCTGGGCTTGATGAAGAATAATTGAAATGATAGTATTAATTACACTGCCTTTGGTAGAGGAAAGTGGTAAataataatcaattgttaaatta                                                                                                                     >  2:105268‑M1/1‑121 (MQ=255)                        tCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatt                                                                                                                   <  1:669152‑M1/143‑25 (MQ=255)                           ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                <  1:681407‑M1/143‑28 (MQ=255)                           ttAACTGCTCATCCTCGCCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                <  2:90304‑M1/143‑28 (MQ=255)                            tAACTGCTTCTCCTCATCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgc                                                                                                               <  2:613037‑M1/143‑29 (MQ=255)                             aacatcttctcCTTACCATGATGTTATTTTCGCCACATCATAATCCTGGGCTTGATGAATAATAATTGAAATCATATTATTAATTCCATTGCACTTGGGATAGGAAATTGCTAAataataatcaattgttaaattattgtgca                                                                                                              <  2:399702‑M1/138‑30 (MQ=255)                               cTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGCAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAATGCTAAataataatcaattgttcagttactgtgcatt                                                                                                            >  2:181817‑M1/1‑112 (MQ=255)                                  cTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttca                                                                                                         >  1:439646‑M1/1‑109 (MQ=255)                                    tctcCTCACCATCATGTTATTTTCGCAACAAAACAAGCCCGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatcgtgcatttcact                                                                                                       >  2:401823‑M1/1‑107 (MQ=255)                                       cctcACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAACGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact                                                                                                    <  2:607073‑M1/143‑40 (MQ=255)                                           aCCAGCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaa                                                                                                <  2:617146‑M1/143‑44 (MQ=255)                                              atcatGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactg                                                                                             >  2:533168‑M1/1‑97 (MQ=255)                                               tcatGGTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgt                                                                                            <  1:622320‑M1/143‑48 (MQ=255)                                                     tATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcag                                                                                      >  2:438636‑M1/1‑90 (MQ=255)                                                         ttCTCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTAGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                  <  2:711768‑M1/143‑58 (MQ=255)                                                          tCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGATAAataataatcaattgttaaattattgtgcatttcaataatggaaatgtaatcagaaaag                                                                                 >  2:511724‑M1/1‑85 (MQ=255)                                                             ccACATCATAGTCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagata                                                                              <  1:719496‑M1/143‑62 (MQ=255)                                                             ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCATTTGGTAGAGAAAAGTGCTAAataataatcaattgttaaattattgtgcatttcaatactggaactgtcatcagaaaagata                                                                              >  2:229718‑M1/1‑82 (MQ=255)                                                             ccACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCAACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattatggtgaatttcactactggaactataataagaaaagaaa                                                                              >  2:403355‑M1/1‑82 (MQ=255)                                                               acaTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagataga                                                                            <  1:25246‑M1/143‑64 (MQ=255)                                                                     tAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaggatagacatgct                                                                      >  1:469504‑M1/1‑74 (MQ=255)                                                                           tGGGCTTGCTAAAGAATAATTGAAATGATATTATTACTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacacccttagcca                                                                >  2:113209‑M1/1‑68 (MQ=255)                                                                                    tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatt                                                       >  2:295177‑M1/1‑59 (MQ=255)                                                                                    tGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatt                                                       >  1:179480‑M1/1‑59 (MQ=255)                                                                                     gaagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattt                                                      >  1:230237‑M1/1‑58 (MQ=255)                                                                                           gagttgaaACGCTATTATTAATTCCACTGGCTTTTGTAGATGAAAGTGCTAAataataatcaattgttaaattagtgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctccatttgattga                                                <  2:536749‑M1/140‑92 (MQ=255)                                                                                              ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaatt                                             >  2:167904‑M1/1‑49 (MQ=255)                                                                                                    tGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattcttgtgcatttcactactggaactataatcagaaaagatagacaagattagaaaaaatcaatttgattgaatagaaaga                                       >  1:93209‑M1/1‑43 (MQ=255)                                                                                                     gATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagat                                      >  1:478330‑M1/1‑42 (MQ=255)                                                                                                          tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttg                                 <  1:616802‑M1/143‑107 (MQ=255)                                                                                                           attaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgt                                <  1:604060‑M1/143‑108 (MQ=255)                                                                                                            ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgtt                               >  2:208372‑M1/1‑35 (MQ=255)                                                                                                             taATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaggatgattggta                              >  1:430216‑M1/1‑34 (MQ=255)                                                                                                                  ccACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgtttaattattgtgcctttcaatagtggcattgtgctcagatatgatagccatgcttagccagtctctatttgattgaattgaaagatgtttgttaaggca                         >  1:124011‑M1/1‑29 (MQ=255)                                                                                                                                    gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagat       >  1:570975‑M1/1‑11 (MQ=255)                                                                                                                                         tGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctga  >  1:471059‑M1/1‑6 (MQ=255)                                                                                                                                              |                                                                                                                                          AATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATT  >  NC_000913/1299357‑1299635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑