Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,277,820	G→T	G118* (GGA→TGA)	soxR →	redox‑sensitive transcriptional activator of soxS; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,277,820	0	G	T	100.0%	61.5 / NA	26	G118* (GGA→TGA)	soxR	redox‑sensitive transcriptional activator of soxS; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/15);  total (11/15)

AAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGAAAGAGTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAG  >  NC_000913/4277692‑4277954                                                                                                                                 |                                                                                                                                       aatcttttCGCGTGTTGCCCCAAGCGCATACGTTACGTGCGAAAGAGTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGTGCTGCGTGACTAACTGGACTGATGTATTGGTtgt                                                                                                                          <  1:135335/138‑1 (MQ=255)                      aaGGGCATACGTTAAGTGCGAAAGAGTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGAt                                                                                                     <  1:78511/143‑1 (MQ=255)                         ggCATACGTTAAGTGCGAAAGAGTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGc                                                                                                  >  2:425039/1‑143 (MQ=255)                              gcGTTACGTGCGATAGATTGGAAACACCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGtt                                                                                             <  2:49415/142‑1 (MQ=255)                              aCGTTAAGTGCGAAAGAGTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGtt                                                                                             >  2:269488/1‑143 (MQ=255)                                      tGCGAAAGAGTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGCCGAACTGGACTGATGTATTGGTCGTGGCTGCCCTTCGCGCAGTGATTGCGCGTTGCCTAAcc                                                                                     >  2:88149/1‑143 (MQ=255)                                      tGCGAAAGAGTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCACGTTGCGTAAcc                                                                                     >  2:85835/1‑143 (MQ=255)                                                    aaCAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAAGCCGCGCGAGCGCTTa                                                                       >  1:407747/1‑143 (MQ=255)                                                    aaCAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGGTTa                                                                       >  1:291537/1‑143 (MQ=255)                                                       aGCGTTCGTCCCAATGGCGAGAAGAGCTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGga                                                                    <  2:246320/143‑1 (MQ=255)                                                           ttCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGACTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGagcag                                                                >  2:316158/1‑143 (MQ=255)                                                               tCCCAATGGCGAGAAGTGTTGGATCGGCGCATTCATACCTTAGTCGCCCCGCCGGACTTACTGGACTGCTGTACTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAgg                                                            <  2:289706/143‑1 (MQ=255)                                                                  cAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAGCCCGGGCGACCGCTTAGGAGAAGAAGGTAc                                                         <  1:126746/143‑1 (MQ=255)                                                                  cAATGGCGAGAAGAGTTGGATCGACGCAATCATACATTAGTGGCGATGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCTAACTGTGATTGCCCGTTGCGTAACCCTGGCGACCGACTAAGAGaagaagatcc                                                         >  2:388621/1‑139 (MQ=255)                                                                              gagTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCtt                                             <  1:267122/143‑1 (MQ=255)                                                                                    gATCGGCGGATTCAGACCTTAGTGGCGTTGCGTGACGAAGTGGTCTGATGTATTGGTTGTGGCTGCCTTTCTCGCAGTGATTGCCTGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGa                                       <  2:39877/143‑1 (MQ=255)                                                                                     aTCGGCGCATTCCTACCTTAGTGGCGCCGCGTGACGAACGGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGCTTGCCCGTTGCGTACCCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGaa                                      <  1:263356/143‑1 (MQ=255)                                                                                     aTCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGaa                                      >  2:49608/1‑143 (MQ=255)                                                                                     aTCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACACGGGTGGCTGGTGGGTAGAAGAAGGTACCGGCGCACGCTTGCTGGaa                                      <  1:333032/143‑1 (MQ=255)                                                                                                  tACCTTAGTGGCGCTGCGTGACGAACTGGACTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGGGACCGCTTAGGGGAAGAAGGTACCGGCGCACGCTTGCTGGTTGATGTACGAAACt                         >  1:256091/1‑143 (MQ=255)                                                                                                     cTTAGTGGCGCTGCGTGACGAACTGGCCTGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGCGAAGCAGGTACCGGCGCACGCGTGCTGGACGATGAACCCAACTcaa                      >  1:185081/1‑143 (MQ=255)                                                                                                          tGGCGCTGCGTGACGAACTGCACTGGTGTATTGGTGGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGGTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGcc                 <  1:95255/143‑1 (MQ=255)                                                                                                                cgCGTGACGAACCGGAGTGATGTATTGGTTGTGGCTGCCGTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAgg           <  1:186099/142‑1 (MQ=255)                                                                                                                  cGTTACGAACTGTACTGAGTTATTGGCGGTGGCTGCGTTTCGGGCAGTGAGTGCCGGTTGCGTAACCCGGGCGACGGCGTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGTAAGATGAACAGAACGAAAGGGCCACAAGGgc         <  2:412382/143‑1 (MQ=255)                                                                                                                      aCGTACTGGACTGATTTATGTTTTTTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCtt     <  1:208735/143‑1 (MQ=255)                                                                                                                         aaCTGGACTGATGTAGTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAg  <  2:344922/143‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       AAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGAAAGAGTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTAGTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGATTGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCTGGAAGATGAACAAAACTAAAGCGCCACAAGGGCGCTTTAG  >  NC_000913/4277692‑4277954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑