Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258674 258683 10–776 71 [0] [0] 70 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)63 (1.330) 53/392 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

AATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCA  >  NC_000913/257680‑258131
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                
aaTTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTA                                                                                                                                                                                                                                  <  1:94154‑M1/228‑1 (MQ=255)
      taggaatcccaTTTACCGCCGTGTATCGGAATTTTGACAAAGGTCTTTAGGAAATAACCGACTAGATCGAAAAACGCTTGTGGTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgga                                                                                                                                                                                                                            <  2:443356‑M1/223‑7 (MQ=255)
          gttccCATTTAACCCGGTTTTTCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaaga                                                                                                                                                                                                                        <  2:325951‑M1/226‑11 (MQ=255)
             cccATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGAAGATTGATCAAAAAATTTACCGAACTAGGCCCGTATATTCGGGAAGGAAagtgcaaaaaaaa                                                                                                                                                                                                                     >  2:569052‑M1/1‑215 (MQ=255)
                          gtgtATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGAAACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGAAGATTCGTGAAGGAAagagcaaaattaaacgattctttttg                                                                                                                                                                                                        >  2:677707‑M1/1‑202 (MQ=255)
                           tgtATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCCGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGAAagtgcaaagataatcgattctttt                                                                                                                                                                                                          >  1:451953‑M1/1‑201 (MQ=255)
                           tgtATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGGTAAAAATTTGCTAAATTGTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgca                                                                                                                                                                                                                            <  1:268707‑M1/207‑7 (MQ=255)
                            gtATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcga                                                                                                                                                                                                      <  1:569052‑M1/228‑29 (MQ=255)
                               tCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGAATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCGGATTGATCAAAAAATTTAACGCCCTAGGCCCGTATATTCGTGAAGGAAagtgcaaagagaatcgattctttttcgatt                                                                                                                                                                                                    >  1:532016‑M1/1‑197 (MQ=255)
                                cGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgt                                                                                                                                                                                                  >  1:454422‑M1/1‑196 (MQ=255)
                                cGGAATTTTGAAAAAGGGTTTCAGGAAATTACCGACTGGATCGAAAAACGGTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgt                                                                                                                                                                                                  <  2:598448‑M1/228‑33 (MQ=255)
                                       ttGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACATGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCCCTAGGCCCGCAAATTCGTGAAGGTAagtgcaaagaaaatcgattatttttcgatggtttggctg                                                                                                                                                                                           >  2:427124‑M1/1‑189 (MQ=255)
                                       ttGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAACTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATTTTCGTGAAGGACagtgcaaagataatcgattctttttcgcttgtctggctg                                                                                                                                                                                           >  1:18608‑M1/1‑189 (MQ=255)
                                                   ttCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGCATATTCTTGAAGGTAagtggaaagataatcgattctttttcgattttctggctgtagacgtaaaag                                                                                                                                                                               >  2:630063‑M1/1‑177 (MQ=255)
                                                       agaAATCCCCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaa                                                                                                                                                                           <  1:162398‑M1/227‑56 (MQ=255)
                                                          aaTCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                                                                                                                                                        >  1:86297‑M1/1‑170 (MQ=255)
                                                            tccccgacTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacc                                                                                                                                                                        <  1:335448‑M1/223‑59 (MQ=255)
                                                                  aCTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGCTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagagcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaacagtgaccgg                                                                                                                                                                >  1:702889‑M1/1‑162 (MQ=255)
                                                                  aCTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGGGAAGGTAagtgcaaagataatcgatttttttttggttttctggctgtatgcgtcaacgtgaaacccgccccgg                                                                                                                                                                >  2:416244‑M1/1‑162 (MQ=255)
                                                                    tGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcggttgtctggctgtatgcgtcaacgtgaaaccggcaccgga                                                                                                                                                               <  1:416244‑M1/227‑68 (MQ=255)
                                                                     gatttaaaaaacgctttTGTTAAAAATTTGTTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATTACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaag                                                                                                                                                             <  2:629833‑M1/222‑70 (MQ=255)
                                                                      gATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACCGGAGATAGCAATGACGTTACCGAGTGGACACCCGACGAGAAGATTGATCAAAACATTTACCGCCATCGGCCCGTATATTCGTGAAGGTAcgtccaaagatactcgtttctttttcggttgcctggctgtatgcgtcacacgaaaacaggcaccgga                                                                                                                                                               >  1:404389‑M1/1‑158 (MQ=255)
                                                                       aTCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtg                                                                                                                                                           >  1:599837‑M1/1‑157 (MQ=255)
                                                                           aaaaaCGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgggaaaccggaaccggaagtgcggg                                                                                                                                                       >  1:566548‑M1/1‑153 (MQ=255)
                                                                                     gtgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgggg                                                                                                                                             >  1:379165‑M1/1‑143 (MQ=255)
                                                                                              aTTTGTTAAATTTTGCCAATTTGGTAAAACAGTGGCATCGAAACAGGAGATAGAAATGCCGTTACGAGGTGGACACCGGAAGAGCAGATTGAACAAAAAATTTACCGCACTAGGCTCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtgtgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggat                                                                                                                                    <  2:333147‑M1/228‑95 (MQ=255)
                                                                                               tttGCTAAATTTTGCCAATTTGGAAAAACAGTTTGATCACAACAGGAGATAGCAATGATGTTGCCGAGTGGACACCCGAAGAACAGATTGATCTAAAAATTTACCGCACTAGGCCCGTTTATTCGTGAAGGTAattgcaaagctaatcgattctttttcgattgtctggctgtatgcgacaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggat                                                                                                                                    <  1:435940‑M1/227‑95 (MQ=255)
                                                                                                 tGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGGAATGACGTTACCGAGTGGACACACGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtaaacgtgaaaccggcaccggcagtgcgtgaattctggggctgggggctgga                                                                                                                                 >  1:632954‑M1/1‑131 (MQ=255)
                                                                                                         tttGCCAATTTGGGAAAACAGTTCCAACACAACAGGAGATAGCAAGCGCGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGTACTAGGCCCGTTTATTCGTGAAGGTAagtgcaaagataatcgattctttgtcgattgtctggctgtatgcggcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaag                                                                                                                         <  2:306980‑M1/228‑106 (MQ=255)
                                                                                                           tGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATTACGTTACCGAGTGGACACCCGAAGAGTAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcg                                                                                                                       <  1:111702‑M1/228‑108 (MQ=255)
                                                                                                               aagttggtAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTTGACCCCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                                                                                                    <  1:419819‑M1/224‑111 (MQ=255)
                                                                                                                 tttGGTAAAACAGTTGCATCACAACAGGATATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaa                                                                                                                 <  1:165018‑M1/228‑114 (MQ=255)
                                                                                                                   tGGTAAAAAAGTGGCATCAAAACAGGAAATAGCAATGTCGTTACCGAGTGGACATCCGAAGAGCAGAGTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggcttgtggatggagctggaagcgcaggaatc                                                                                                               <  2:532016‑M1/228‑116 (MQ=255)
                                                                                                                       aaaaCAGTTGCATCAAAAAGAGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcggcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgt                                                                                                           <  2:97001‑M1/228‑120 (MQ=255)
                                                                                                                                     acaacggagaatagcaaagcgGTTACCGAGTGGCAACAAGGAGAGCACAGTGATCAAAAAATGTACCGCACTAGGCTCGTATAGTCGGGAAGGTAagtgcaaagataatcgattctttttcgagtgtctggctggatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggggctggaagcgcaggaatcccgttttacctacagtta                                                                                             <  2:566548‑M1/218‑134 (MQ=255)
                                                                                                                                      gagacagaaatagaaatgaCGTTCCCGATGGGACACACGAAGAGCAGATTGATCAAAAAATTTACCGCCATAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagtta                                                                                             <  1:701942‑M1/218‑134 (MQ=255)
                                                                                                                                      caacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattc                                                                                                                                                  >  1:184243‑M1/1‑94 (MQ=255)
                                                                                                                                       aacaGGAGATAGAAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCATTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattct                                                                                                                                                 <  2:184243‑M1/174‑82 (MQ=255)
                                                                                                                                               atagaattgctgttcccgtgtggAAACCCGAAGAGCAGATTGATCAAAAAATTTACCGGTCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgtttgtctggctgtatgcgtcaacgtgaagccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt                                                                                   <  2:43806‑M1/217‑144 (MQ=255)
                                                                                                                                                 aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttccgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaag                                                                                                                         >  1:404535‑M1/1‑83 (MQ=255)
                                                                                                                                                 aGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttccgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaag                                                                                                                         <  2:404535‑M1/188‑106 (MQ=255)
                                                                                                                                                  gatatgtCGTTCCGGAGTGGACACCCGAAAGGGAGATTGAACAAAAAATTTACCGCACTAGGCCCGGATAGTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctg                                                                                <  2:275461‑M1/225‑147 (MQ=255)
                                                                                                                                                  acaATGGCGTTCCCAGGGGGACACCCGAATAGGAGAATGAACAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctg                                                                                <  2:379165‑M1/227‑147 (MQ=255)
                                                                                                                                                   cAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgtggctggtggatggagctggaagggcaggaatcccgttttacctacagttaccagtttgg                                                                                    >  1:43806‑M1/1‑81 (MQ=255)
                                                                                                                                                      tGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTAGATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgaatgcgtcaacgtgaaaccggcaccggactatcgtgaattctggggctgtcggttgtagctggaagcgcaggaatcccggttttcctacagttaccactttggtctgttgg                                                                            >  2:460959‑M1/1‑78 (MQ=255)
                                                                                                                                                        aCGTTACCGAGTGGACACCCGAAGAGCAGATTGATAACAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGGAagtgcaaagataatcgattctttttcgattgtctggctgtatgggtcaacgtgaaaccggcaccggaagtgcgtgagttcttgggctggtggatggagctggaagcggaggaatcccgtttttgctgcagttaccaggttggtgttgtc                                                                             >  2:701942‑M1/1‑76 (MQ=255)
                                                                                                                                                            tACCGAGTTGACACCCGAATAGCAGTTTGAACAAAAAATTTACCGCTTGAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttgtcgatggtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaag                                                                      <  1:334129‑M1/228‑157 (MQ=255)
                                                                                                                                                                 aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCTCTAGGCCCGTATATGCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggc                                                                 <  1:427124‑M1/228‑162 (MQ=255)
                                                                                                                                                                               gagCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaaccgcaggaatcccgttttacctaccgttaccagtttggtctggtcgataaagcaggcgactgaaagagtgt                                                   >  2:404716‑M1/1‑53 (MQ=255)
                                                                                                                                                                                 gcaaattttccaaaaaatttaCCGCACTAGGCCCGTATATTCGTTAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttc                                                 <  1:316842‑M1/218‑178 (MQ=255)
                                                                                                                                                                                  cAGATTTATTAAAATATTTACCTCATTAGGCCCGTATATTCGGGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtaggcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttc                                                 <  2:207765‑M1/227‑178 (MQ=255)
                                                                                                                                                                                  cAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagggttc                                                 >  1:572825‑M1/1‑50 (MQ=255)
                                                                                                                                                                                  acgatTGATCAAAAAATTTCCCGCTCTGTGCCCGTATATGCGTGAAGGTAagtgcaaagataatcgattcgttgtcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttc                                                 <  1:677707‑M1/225‑178 (MQ=255)
                                                                                                                                                                                    gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagaggtttc                                                 >  1:460689‑M1/1‑48 (MQ=255)
                                                                                                                                                                                    gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagggcaaagataatcgcttctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattttggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttcacagtttgggctgttcgataaacgagggtcctggaagaggttaccgg                                              >  2:290370‑M1/1‑48 (MQ=255)
                                                                                                                                                                                     gttgATAAAAAAATGTACCGCACTAGGCCCGTATATTCTGGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaaga                                                       <  1:290370‑M1/217‑172 (MQ=255)
                                                                                                                                                                                     aTTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatc                                                                                                               <  1:282131‑M1/162‑116 (MQ=255)
                                                                                                                                                                                            aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctccagttaccagtttggtctgttcgttaaagcaggggactggaagagggttccggtaaaagac                                      >  1:516685‑M1/1‑40 (MQ=255)
                                                                                                                                                                                                   ttACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggctggagctggaagcgcaggaatcccgttttacctacagttcccagttgggtctgttcgataaagcaggcggcgggcagagggtcccggaaaaagaaactgaag                               >  2:603046‑M1/1‑33 (MQ=255)
                                                                                                                                                                                                    gtccgcCCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaa                                <  1:603046‑M1/224‑195 (MQ=255)
                                                                                                                                                                                                              ggCCCGGATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacga                    <  1:630063‑M1/228‑207 (MQ=255)
                                                                                                                                                                                                                cccTTATTTTCGTGAAGGTAagtgcaaagataatcggttctttttcgagtgtgtgggtgtatgcggcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgact                  <  2:572825‑M1/228‑209 (MQ=255)
                                                                                                                                                                                                                   gTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcggtaaagcaggcgactggcagagtgttccggtaaaagacactgaagtggttgtacgactgga               >  1:233253‑M1/1‑17 (MQ=255)
                                                                                                                                                                                                                    tataTTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggaaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagattggtctgttcgataaagcaggcaactggaagagtgttccggtaaaagcaactgaagtggtggaaggacgggag              >  2:582192‑M1/1‑16 (MQ=255)
                                                                                                                                                                                                                      tatTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgactggagca            >  1:177250‑M1/1‑14 (MQ=255)
                                                                                                                                                                                                                         tCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggttggagctggaatcgcaggcatgccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccgggaaaggcacttgacgtggtttaacaacgggtgccaac         >  1:192182‑M1/1‑11 (MQ=255)
                                                                                                                                                                                                                          cGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggcctgttcgctcaagcaggcgactggaagagtgttacgggaaacgcacctgaaatggttgaacgactggagcacacc        >  1:331607‑M1/1‑10 (MQ=255)
                                                                                                                                                                                                                          cGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctgggggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgcctgggccacacc        >  1:498317‑M1/1‑10 (MQ=255)
                                                                                                                                                                                                                              aaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacga                    >  2:546393‑M1/1‑6 (MQ=255)
                                                                                                                                                                                                                              aaGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacga                    <  1:546393‑M1/212‑207 (MQ=255)
                                                                                                                                                                                                                                ggTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggtctgttcgataaagcaggcgactggaagagtgttccggtaaaagacactgaagtggttgaacgcctggagcacacccggcgt  >  1:490839‑M1/1‑4 (MQ=255)
                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                
AATTATTAGAGATCCCATTTAACCCGGTGTATCGGAATTTTGACAAAGGTCTTCAGGAAATCACCGACTGGATCGAAAAACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCA  >  NC_000913/257680‑258131

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: