Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,913,613 | A→C | 15.3% | G13G (GGT→GGG) | relA ← | (p)ppGpp synthetase I/GTP pyrophosphokinase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,913,613 | 0 | A | C | 15.3% | 196.2 / 2.6 | 87 | G13G (GGT→GGG) | relA | (p)ppGpp synthetase I/GTP pyrophosphokinase |
Reads supporting (aligned to +/- strand): ref base A (35/35); new base C (3/14); total (38/49) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.72e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTC‑AATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGG > NC_000913/2913387‑2913838 | ggTTGGATTCCCCCCGGGGGAAAAGTCGCGCCGCCCGCAGTCTCTC‑AATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCATGCCACAATAACAGACTGGCTTCCGGATGCCCCTGCGTCTGTTGCAGTCAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCAc < 2:299403/227‑1 (MQ=255) cggtGGCATCCCCCAGAGGGAAAAGCCGCGCCGCCCGAAGCGTGTC‑AATGTCCATACTTAAAGTCGAGAGGATCCCCCCCTTCTCACCACCCCGCCACAATAACAGACTGGCATCCAGATGCCCCTGCTTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATCCCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCAc < 1:691840/225‑1 (MQ=255) gCAACCCCCAGAGGGAAAAGCAGCGCCCCCCGCAGCGTGTC‑AATGCCCATACTTAAGGTCGAGAGGATCCCCACCATCCCAACCCCACGCCACAATAACAGGCTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGGTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGcc < 2:574968/228‑1 (MQ=255) cATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTC‑AATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATCCCCCTGCCTCTGTTGCAGACACTACGCCCAGGTTTCGGCTAAGCACTCACCCGACTCC‑TGGCCTGTAATACCCAGACTTCCGATCCCATTTTCCGGCAC‑ACATTCAcccaccc > 2:36868/1‑223 (MQ=255) cGCCAGAGGGAAAAGCCGCCCCGCCCGCAGCGTGTC‑AATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTAtt < 1:255809/228‑1 (MQ=255) gCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTC‑AATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACCGACTGGCATCCGGATGCCCCTGCGTCTGTTTCAGACAATACGCCCAGGCTTCGGCCAAGCACTCACCAGACTTC‑TGGCGGGCAATACCCAAAATTTCGATCCATTTTTCCGGACC‑ACATCACCCAGccacaatt > 2:446935/1‑222 (MQ=255) tgAAAAGCAGCACCTCCCCCAGCGTTGC‑AATGTCCATGCTTAAGTTCAAGGTCATCGTCACCATCCCAAACCCACGCCACAATTACAGACTTGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTACTGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATAtgt < 1:436791/226‑1 (MQ=255) gcaaacccgccccgcccccAGC‑TGTCAAATGCCCATCCCTAATGCCGAGAGGATCGCCCCCAGCTCAACCCCCCGCCAAAATAACCGACGGGCCTCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGc < 2:690739/217‑1 (MQ=255) ggccgaggCGTGGC‑AATGTCCAAACCTAATGGCGAGAGGAACTCCACCATCCCAACACCACGCCACAATACCAGGCTGGCCTCCGGATGCCCCGGCGTCTGTTGCAGACAATCCGCCCAGGTTTCGGCTAAGCACTCGCACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGc < 1:469671/220‑1 (MQ=255) cccgcAGCGTGTC‑AATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCCGCTAAGCACTCCCACGACTTC‑TGGCTGGAAATACCCCGACTTGCGATCCATTTTTCCGGCTC‑AAATTACCCAACCTTATTGATATGGGCACTTCTTTACGCa > 2:265233/1‑227 (MQ=255) gccgCGTGTC‑AATGCCCATCCTTAATGTCGAGAGGATCTCCACCCACTCAACCCCACGCCACAATAACAGGCTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCa < 1:655145/225‑1 (MQ=255) ttgtC‑AATGTCCATCCTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTc < 1:238061/227‑1 (MQ=255) gtC‑AATTTCCATACTTAATGTCAAGGGGATCTCCACCCTCCCAACACCAACCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCct < 1:265233/228‑1 (MQ=255) gtC‑AATTGCCATACTTAATGTCGGGGGGATCTCCACCCTTCCAACCCCACGCCCAAATAACAGACTGGCCTCCGGATGCCCCTGCGTCTGTTGCAGACAATTCGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTCGCCGCACCCATCGACct < 2:206619/228‑1 (MQ=255) gtC‑AATGTCCATACTTAAGGTCGAGAGGATCCCCCACATCCCAACACCACGCCACAATAACAGACTGGCATCCGGAGGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCct < 1:294014/228‑1 (MQ=255) aaTGCCCATACTTAATGTCGAGAGGATCCCCACCATCTCAACACCACGCCACAATAACAGGCTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCtctc < 1:315499/228‑1 (MQ=255) cTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAAAACGCCCAGGCTTCGGCTAAGCACTCCCACGACTTC‑TGGCTGGTACTACCCAGACTTGCGATCCATTTTTCCGGCTC‑AAATTCACCAGCCTTATTGATTTGTGCACTTTTTACCGCAACCAACGCCCCCCCCTTTTGGGAc > 2:198720/1‑228 (MQ=255) aaTGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGa > 2:467147/1‑181 (MQ=255) aTGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGat < 1:467147/181‑1 (MQ=255) agagGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCa > 2:404683/1‑161 (MQ=255) agagGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGCTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑CAATTTACCAGCCTTATTGCTATTTGCACTTCTTACCGCAACCATCTCCCTCCCCTTTTGGGACCAGCCCCGcc > 2:8224/1‑228 (MQ=255) agagGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATCCCCCTGCGTCTGTTGCAGACAAAACGCCCAGGCTTCGGCTAACCACTCACACGACTCC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTCATCGATATGTGCCCTTCTTACCCCAACCAACGCCCCCCCCTTTAGGGCCCAGCCCTccc > 2:664707/1‑228 (MQ=255) gagGATCTCCAACATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCAc < 1:404683/161‑1 (MQ=255) ccccaTCCCAACCCCACGCCAAAATAACAGTCTGGCATCCGGATGCCCCTGCGTCTGGTGGAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGTTCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCa < 1:527991/226‑1 (MQ=255) tctcAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGCCCAGACCCGCCTAAACCGGCaaaac > 2:489235/1‑225 (MQ=255) tccaccccaacccAAAATAACAGCCGGGCCTCCGGATGCCCCTGCGTCTGTTGCAGACAATCCGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATCCCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCa < 1:8224/217‑1 (MQ=255) aaCACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTcc > 1:662508/1‑185 (MQ=255) cGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGCTCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGGTATGTGCACTTCTTACCGCAACCATCCTCCTCCCCTTTATGGACCAAACCTGCCGAAATCGGCAAACCGCAACAATTTaa > 2:457447/1‑228 (MQ=255) cGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCTAAATCTGCAAATCGCAACTAtttca > 1:664079/1‑226 (MQ=255) cGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTaa > 1:545535/1‑228 (MQ=255) cGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGACCTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGTCAAATCCCAACTATTTaa > 1:607961/1‑228 (MQ=255) cGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGCTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTCCCTCCACCATCTTCCTCTCCTTTATGGACCAGACCTGCCGAAATCGCAAAATCGCAACCAtttta > 2:438950/1‑226 (MQ=255) ccACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAAcg < 1:484324/228‑1 (MQ=255) caATAACAGCCCGGCCTCCGGATGCCCCTGGCTTTTTTTGAGACAATACGCCCAGGTTTCGGCTAAACACTCACCCGAATTT‑GGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCCCCAGCCTTATTGAAATGTCCACTGCTAACCGAAACAATCGTCCTCTCCTTAAGCGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAAcgcgc < 2:306374/228‑1 (MQ=255) aaccagtccggCACCCGGATGCCCCTGCGTCTGTTGCAGAACATACACCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATCCCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCgag < 1:330544/219‑1 (MQ=255) gcatagtGGCTTCCGGATCCCCCTGCGGCGGTTGCAGACAATCCCCCCAGGTGTGGGCTAACCACCTCCACGGATTC‑TGGCTGGTAATACCAAGACTTGCGATCCATTTTTCCGGATC‑AAATTCCCCAGCCTTATTGTTATGGGCACTTCTTCCGGCAACCATCGTCCCCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGaa < 2:399460/222‑1 (MQ=255) tGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCACCCATCGGCCTCTCCTTTAGGGACCAGACCTGCCGAACTCGGCAAATCCGACCTATTTAAcgcgcccgtaaatga > 1:306374/1‑217 (MQ=255) tGGCATCCGGATGCACCTGCGTCTGTTGCAGACAATACGCCCCGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTTCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAAACGGCAAATCCAAAATCTTTAACACGGGAGcaaaaaac > 2:418253/1‑224 (MQ=255) gCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCCACAATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATATAAAGCGCGGGAAAAGtcacc > 1:652231/1‑224 (MQ=255) gCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGCAATACCCAGACTGGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGACCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTAc > 1:519231/1‑226 (MQ=255) gATGCCCCTGCGTCTGTTGCAGACAATCCGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTc < 1:210468/228‑1 (MQ=255) acagCCCCTGCTGCTTGTGCAGACAATTCGCCCAGGTTTCGGTTAAACACTCACACGACTTC‑TGGCTGGTAATACCAAGACTTGCGATCCATTTTTCCGGATC‑AAATTCCCCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTc < 1:298905/225‑1 (MQ=255) gtGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTGCGCTAAACCCTGACCACACTTC‑TGGCTGGTAATACCCATCTTTGCGATCCA‑TTTTCCGGATC‑AAATTCCCCAGCCTTATTGATAGGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTTCCATCGAt < 1:705042/223‑1 (MQ=255) acccccTTCGTTTGTTGCGAACAATACGCCCTGGGTCCGGCTACACAGTTACACGCTTTC‑TGGCTGGTAATACCCAGCGTTGCGATCCATTTTTCCGGATC‑AAATTCCCCAGCCTTATTGATATGTACCCTTCTTACCTCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCa < 2:248953/226‑1 (MQ=255) cccTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGAt > 1:16769/1‑228 (MQ=255) tgggTCTGTTGGAAACAATTCGACCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATTCCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc < 1:247262/225‑1 (MQ=255) tGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGCCTTC‑TGACAGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCCCCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGACCTCTCCTTTAGGGAACAGCCATGCCGAAATACGCACATCGCCACCATTTAACACGCGAGAAAAGTACCAACGCTTTCAgcggtc > 2:441852/1‑223 (MQ=255) tGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTc > 1:6373/1‑228 (MQ=255) cGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACCATTTACCGCCCCATCCACGTACACTCGATTCCAAATGGccc > 1:248953/1‑228 (MQ=255) tGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAAcgcg > 1:237787/1‑193 (MQ=255) gTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCACCTATTTAACGCCCGAGAAAAGTACCATCGGTTCCAGATGTccccggt > 1:80949/1‑223 (MQ=255) gTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTGC‑TGGCTGGTAATACCCAGACTTGCAATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCCATCCTTTAGGGACCAGACCCGCCGCAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAATAACATACATCCCAGacggcacacaga > 2:298905/1‑217 (MQ=255) tgccaacaacaCGCCCCGGTTTCGCCTAACCCTTCCCCCGATTTT‑GGGCTGGTAATCCCCAGACTTGACCTCCATTTTCCCGGATC‑AATTCCACCAGCATTATGGATATGTGCCCTTCTTACCGCAACCATCGCCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtg‑tg < 2:521022/223‑1 (MQ=255) tgaaGACAATACGCCCAGGTTTCGGCTAAGCACTCGCACGGCTTC‑AGGCTGGTAATCCCCAGGCTTGCGATCCATTTGTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtg‑tg < 1:438950/225‑1 (MQ=255) tGCAGAAAAATCGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATTCCCAGAATTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGtgtg‑tg < 1:489235/228‑1 (MQ=255) agggacagctcccATGTTTTGGCTAAGCTCTCCCCCATCTTC‑TGGCTGGTAATCCCCTGGCTTGCGATCCTTTTTCCCGGATC‑AAATTCCCCACCCTTATTGATATGTGCACTTCTTACCCCAACCATCGCCCCCTCCTTTAGGGCCCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGa < 2:256032/218‑1 (MQ=255) ggTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTCTTTAACGCTCCAGAAAAGTACCATCGATTCCAAATGTCCCGTGTG‑TGGGAACTTAACAAACATc > 2:3927/1‑228 (MQ=255) ggTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGCAAAGTACCATCGATTCCAGATGTCCCG‑GTGTTGGGAACATATCCAGCATc > 1:483392/1‑228 (MQ=255) ggTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGCGTG‑TGGGAACATATCTAGCAtg > 1:375297/1‑227 (MQ=255) cGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGcctgtcta > 1:256032/1‑220 (MQ=255) cGGCCAAGCCCTCACACGACTTC‑TGGTTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATCCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAg < 1:3927/228‑1 (MQ=255) ggCTAAGCCCTCACACGATTTC‑GGGCTGGTAATCCCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGt < 1:446935/228‑1 (MQ=255) cTAAGCCCTCACCCGGCTTC‑TGGCTGGTAATTCCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCCCCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTcg < 1:171431/228‑1 (MQ=255) tcccTCACCGGACTGT‑TGGTTGGTAATACCCAGACTTGAGATCCATTTTTCCGGATC‑AAATCCACATGCCTTATTGATATGTGCCCTCCTTACCGCAACCATCGTCCTCTCCTTGCGGGACCAGACCGGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCa < 2:16769/225‑1 (MQ=255) gccatcccacGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTCCCGGACC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCGCCTTTAGGAACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGGCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCa < 1:408815/221‑1 (MQ=255) ctcTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTGATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGTAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCa < 1:36868/225‑1 (MQ=255) cacacacaCGACTCC‑TGGCGGGTAATACCCAGACTTGCTATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATGGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTcgcg < 1:677268/225‑1 (MQ=255) cACTCGCACGACTTG‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCCCCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCa < 1:457447/227‑1 (MQ=255) tCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGTGAGAAAAGTACCATCGATTCCAGATGGCCCGTGGG‑AGGGAACATATCCAGCACCCCCCGTcgcac > 2:408815/1‑221 (MQ=255) gACTTCATGGCTGGAAAGA‑CCATACTTGCCATTCTGTTTGCGGGGAC‑ATATTCACCAGACGTAGAGATGTGTGCCCGTCTTACCGCAACCATCATCCTCTTCTTGAGGGACCAGACATGCAGAAAGCGGCACATCGCAGCTATTTAACGAGCTAGAAAAGTACCATCGATTCCAGATGTCCTGTGTG‑TGGGAACATATCCATCATCGCCAGTCGCGCAATTGTATATc < 1:307173/228‑1 (MQ=255) gACTTC‑TGGCTGGTAATGCCCAGACTTGCGATCCATTTTTCCGGATC‑AAAGTCACCAGCCTTAGTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTAAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATc < 1:575469/228‑1 (MQ=255) accTTT‑TGGTTTGTAATACCCATCTTGGCGATCCATTTTTCC‑GATCAAAATTCCCCAGCCTTATGGATACGTGCACTTCTTACCGCAACCATCGGCCTCTCCTTTAGGGCCCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATc < 2:483392/226‑1 (MQ=255) tC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAACTCGGCAAATCGCACCTATTTAACGCGCGAGAAAAGAACAATCGCTTCCAAATGTCCCCTGTG‑TGGGAACATATCAAGCAGCCCAATCCGCCCATTGGAATATcccg > 2:2474/1‑225 (MQ=255) c‑tgggtggTAATCCCCAGATTTGCGATCCATTTTTCCGGATC‑AAATTCCCCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCt < 1:664707/228‑1 (MQ=255) c‑tggctgtTAATACACAGCCTTCCCATCCATTTTTCCTGATC‑AAATGCCCCAGCCTTAGTGATAGGTGCACTTCTTACCTCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCt < 1:339328/228‑1 (MQ=255) tggctggTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGTTGTCCCGTGTG‑TGGGAATATATCCAGAATCGCCAGTCCCGCAATGGCAtctctgtct > 1:271249/1‑222 (MQ=255) gTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGa > 1:121999/1‑157 (MQ=255) gTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGAGGTCCCGGGTG‑TGGGAACATATCCAGCATCCCCCGTCCGGCAATGTAATATCCGGCTTTtaaa > 2:430421/1‑226 (MQ=255) tAATCCCCAGCCTTGCCATCCATTTTTCCGGATC‑AAATTCCCCCGCCTTATTGATATGGGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGAt < 2:121999/157‑1 (MQ=255) tAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTGTtaata > 1:609747/1‑227 (MQ=255) atccccAGGCTTGCGATCCATTTTTCCGGATC‑AAATTCCCCAGCCTTTTTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTTCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACg < 1:638490/225‑1 (MQ=255) aTACCCAGACCTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCCCTTCTTACCGCAACCATCGGCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACg < 1:2474/228‑1 (MQ=255) gATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGCATATCCTGCTTTTAATAACGCTTCGCTATCCCGa > 1:178088/1‑228 (MQ=255) cATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGa > 1:220122/1‑136 (MQ=255) ggACC‑AAATTCACCAGCCTTATTGATATGTGCCCTTCTTACCGCAACCATCGCCCTCCCCTTTAGGGCCCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCa < 1:430421/227‑1 (MQ=255) gATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCACATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGCGTG‑TGGGAACATATCCAGCAACGCAAGTCGCGCAATGGAGTATACTGCTTTTAATAACGCCTCCCTACCCCGACCCAGCGTTGCAgg > 2:114998/1‑228 (MQ=255) ttCCCCAACCTTTTTTATATGGCCCCTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGCCCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAg < 1:114998/228‑1 (MQ=255) tCAACACCCTTAGTGATATGTGCACTTCCTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAgg < 1:263045/228‑1 (MQ=255) | CGTTGGCATCCGCCAGAGGGAAAAGCAGCGCCGCCCGCAGCGTGTC‑AATGTCCATACTTAATGTCGAGAGGATCTCCACCATCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTTCGGCTAAGCACTCACACGACTTC‑TGGCTGGTAATACCCAGACTTGCGATCCATTTTTCCGGATC‑AAATTCACCAGCCTTATTGATATGTGCACTTCTTACCGCAACCATCGTCCTCTCCTTTAGGGACCAGACCTGCCGAAATCGGCAAATCGCAACTATTTAACGCGCGAGAAAAGTACCATCGATTCCAGATGTCCCGTGTG‑TGGGAACATATCCAGCATCGCCAGTCGCGCAATGGTATATCCTGCTTTTAATAACGCTTCGCTATCCCGAGCCAGCGTTGCAGGGTTACAGG > NC_000913/2913387‑2913838 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |