Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.83.9% 175.9 / 19.1 56pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (6/3);  new base . (41/6);  total (47/9)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*NC_0009132,173,3620C.84.2% 173.8 / 16.0 56pseudogene (1/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base . (41/6);  total (47/9)
Fisher's exact test for biased strand distribution p-value = 1.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACC  >  NC_000913/2173127‑2173597
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                            
tAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGAAaccaccgcc                                                                                                                                                                                                                                           >  1:186470/1‑236 (MQ=255)
  gccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGTTAAAAATCAGGCTTGCCGCTaccaccgccgt                                                                                                                                                                                                                                         >  1:1045686/1‑234 (MQ=255)
       cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACCCAAACAGCAATTAAAATGGTGAGGGGGTTAAAAATCAGGCTTGCCGAAACCAcgcccgt                                                                                                                                                                                                                                         >  1:1211688/1‑236 (MQ=255)
               tgtgCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTTCCGAAAC‑‑CCCCGCCGTATGTCCCagcagc                                                                                                                                                                                                                          >  2:880455/1‑239 (MQ=255)
                         aaTATTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACCCCCGAGTTTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAt                                                                                                                                                                                                                <  1:149893/239‑1 (MQ=255)
                                tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGGTTGTCCCAGCAGCAAGGCCgcgccaagccc                                                                                                                                                                                                         >  1:241911/1‑229 (MQ=255)
                                  cGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGAAGCAACGCCGGATCAAGGCCa                                                                                                                                                                                                        >  1:282561/1‑238 (MQ=255)
                                       cgccccACAGGGTGATTCCCATAATGATGACCCCCGAGATTAAGGTGCG‑‑‑‑‑AAACAGATTCCCACGATGACCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg                                                                                                                                                                                                   <  1:969122/234‑1 (MQ=255)
                                                   tGATGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGAAACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAcc                                                                                                                                                                                      >  1:491165/1‑238 (MQ=255)
                                                    gATGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACCCAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGTACCCACCGCCGCATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccg                                                                                                                                                                                     >  1:871493/1‑238 (MQ=255)
                                                    gATGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGGGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCCGCAAAGCCGGCTCAAGGCCAAACAGGAACCCCTGaaca                                                                                                                                                                                      >  2:879099/1‑235 (MQ=255)
                                                     aTGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgcc                                                                                                                                                                                   >  1:4443/1‑239 (MQ=255)
                                                      tGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTccctgtacctt                                                                                                                                                                                   >  1:142647/1‑230 (MQ=255)
                                                      tGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACCCCTAAccgccg                                                                                                                                                                                  >  1:332718/1‑239 (MQ=255)
                                                      tGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGCATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAAGTCCGTAcccccg                                                                                                                                                                                  >  1:236447/1‑239 (MQ=255)
                                                           aTAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACGGCACTTAAAATGGTGCGTGGGCTAAAAATCAGCCTTCCCGAACCCCCCGCCGATGGTCCAGGCAGCAAAGCCGGATCGAGGCCAACCAGAAAACCCTACCCCCCGaaat                                                                                                                                                                              >  2:934915/1‑236 (MQ=255)
                                                           aTAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTAACCCCCGAACt                                                                                                                                                                              >  1:328510/1‑238 (MQ=255)
                                                             aaTGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTc                                                                                                                                                                            >  1:250114/1‑238 (MQ=255)
                                                                  tgaCACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCCACAAAACAGAAATTAAAATGGTGAGGGGGATAAAAATCAGGCTTCCCGATACCACCC‑‑CCGTATGCCCCAGACGCAAGCCCGAAACAGGACCACTACGAACTCCTAAGCCCCCGACCCTCGCCTga                                                                                                                                                                      >  2:1238804/1‑238 (MQ=255)
                                                                   gaCACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTa                                                                                                                                                                     >  1:66222/1‑239 (MQ=255)
                                                                        ccGCGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACACAAACAGCAATTAAAATGGTGAGCGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCCGCAACGCCCGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACg                                                                                                                                                                 >  1:81862/1‑238 (MQ=255)
                                                                                      cG‑‑‑‑‑GAACCGATTTCCACGATGCACGGAGACCGCCCTCGACACGAAGAAGACGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCGCCCAAACAGCCATTACAATGGAGCGTGGGGTAAAAATCCGGCTTGTCGCTACCA‑‑CCGCCGATTGTCCCAGAGGCAAAGCCGGTTCAAGGCCAATATGGAACTCCTGACCGCCGACATTTGCCTGTACACGACCAcacacctcctt                                                                                                                                                  >  2:224773/1‑229 (MQ=255)
                                                                                      cG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCCCACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGCTCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCCCCTGAAAACACCTACGAAccgtcct                                                                                                                                                  >  2:1090771/1‑234 (MQ=255)
                                                                                                 agtttcccACGATCCACGGCGCCCGCCATCGCCACGAAGAAGCCGATGGTGGAAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACAAAAACAGCAATTAAAAGGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                            <  1:1090771/233‑1 (MQ=255)
                                                                                                          cGACGCGCGGCGCCCGCCCCTCCCAAGAAAAAGCCGACGGTGGGCAGAATCGAGAAACGGAGCTCATGAATTCCCCGCCCAACAAACAAAATTTAAATGGGGGGGTGGGAAAAAAACCGGGCTGCGCGTTAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCAAATCCGGAACTCCTGACCGCCGATCTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTa                                                                                                                                    <  2:949782/239‑1 (MQ=255)
                                                                                                             tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGCGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGCGAGTGGGATAAAAATCAGGCTTGCCGCAACCA‑‑CCGACGTATGTCCACGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCCCCGAACTCCGCCCGTAAACGACGAAGAGCCGCCTTAGCGAGGGGGGTAAAAca                                                                                                                                >  2:686013/1‑238 (MQ=255)
                                                                                                               cACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcca                                                                                                                               >  1:1040657/1‑238 (MQ=255)
                                                                                                                    cGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatg                                                                                                                         >  1:895065/1‑239 (MQ=255)
                                                                                                                              cGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa                                                                                                               >  1:281528/1‑239 (MQ=255)
                                                                                                                                   cGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAc                                                                                                           >  1:71704/1‑238 (MQ=255)
                                                                                                                                           ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGGTAAACCATCCAGGATGGGTTTAATAACCCGGGGCAt                                                                                                  >  2:981990/1‑239 (MQ=255)
                                                                                                                                             ggtggggggCAAGACCGCAAAACGGCAGCCCCGTATTACCCGGCACAAAAAAAGCAATTAAAATGGGGAGTGGGATAAAAAGCAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCa                                                                                                   <  1:156374/230‑1 (MQ=255)
                                                                                                                                                ggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGCTGGGCGTTAAACCATCCATTCTGGGTTTAATCACCCGTGGCATTagca                                                                                             >  1:599567/1‑239 (MQ=255)
                                                                                                                                                              ccAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCagcagc                                                                                                                                                                                                                          >  2:93110/1‑102 (MQ=255)
                                                                                                                                                              ccAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCagcagc                                                                                                                                                                                                                          <  1:93110/102‑1 (MQ=255)
                                                                                                                                                                         caCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTAcc                                                                      >  1:815209/1‑238 (MQ=255)
                                                                                                                                                                           ccTGATTCCCCGGACCAAAAAAACAAATAAAAATGGTGGGGGGAAAAAAAATCGGGCTTGCCGAACCCCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACCCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                   <  1:981990/239‑1 (MQ=255)
                                                                                                                                                                                    cccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCACCATCACTGCCGCCGTTTTTACCGCCAGCTGCaa                                                           >  1:825902/1‑239 (MQ=255)
                                                                                                                                                                                       ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCAGGATGGGTTTAATCACCCGTGGCTTTAGCAGCATCACTGCCCCCGTTTTTACCGCCAGCTGCAATac                                                        >  1:22748/1‑239 (MQ=255)
                                                                                                                                                                                         ggcaaaaaaCAGACAATTAAAAGGGTGGGTGGAATAAAAACCAGTCTGCCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATac                                                        <  1:427962/233‑1 (MQ=255)
                                                                                                                                                                                         cacacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTTTAAACGACTACCAGCCTGCTTACCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCTTGGCATTAGCCGCCGCAATGCCCCCATTTTTACCGCCACATGCaaaccc                                                       >  2:544156/1‑234 (MQ=255)
                                                                                                                                                                                         cacacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACCGCCGCCGTTTTTACCCCCACCTGCaaacaa                                                       >  2:758736/1‑234 (MQ=255)
                                                                                                                                                                                            acaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGCTACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTTACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCtt                                                   >  1:1020658/1‑239 (MQ=255)
                                                                                                                                                                                               aaaCAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCACCTGCAATACACCTTTg                                                 >  1:304835/1‑239 (MQ=255)
                                                                                                                                                                                                    gCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATa                                                         >  1:149089/1‑226 (MQ=255)
                                                                                                                                                                                                      cAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCg                                           >  1:586175/1‑239 (MQ=255)
                                                                                                                                                                                                        aTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACGCCCAGCTGCAATACACCTTTGACATCGTa                                         >  1:797362/1‑239 (MQ=255)
                                                                                                                                                                                                        aTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTa                                         >  1:479582/1‑239 (MQ=255)
                                                                                                                                                                                                        aTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCCCCGTTTTTACCGCCACCTGCAATACACCTTTGACATCgca                                         >  1:75628/1‑237 (MQ=255)
                                                                                                                                                                                                             aaTGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAccc                                     >  1:721231/1‑238 (MQ=255)
                                                                                                                                                                                                                ggTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGAc                                               >  1:6969/1‑225 (MQ=255)
                                                                                                                                                                                                                    aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGAt                             >  1:1023423/1‑239 (MQ=255)
                                                                                                                                                                                                                    aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAAGGGCGCTAAACCATCCATGATGGGTTTAATCACCCGTGGAATTACCAGCATCCCTTCCGCCGCTTTTACCGCCAGCCGCAAAACCCCTTTGAAAACGAAACCcccggg                                >  2:1057523/1‑234 (MQ=255)
                                                                                                                                                                                                                    aGTGGAAAAAAAACCGGGCTTGCCGATCCCCCCGCCGTATGTCCCAGAAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGa                              <  1:879099/238‑1 (MQ=255)
                                                                                                                                                                                                                           tAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGCGGATGCCGCTa                      >  1:419317/1‑239 (MQ=255)
                                                                                                                                                                                                                           tAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCAAGGATGCCGTTa                      >  1:413530/1‑239 (MQ=255)
                                                                                                                                                                                                                                   aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCg                                                                                                                                              >  1:162066/1‑111 (MQ=255)
                                                                                                                                                                                                                                         gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcac        >  1:936852/1‑239 (MQ=255)
                                                                                                                                                                                                                                         gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCCAGGATGCCGATAATCAGCCCCATcac        >  1:120666/1‑239 (MQ=255)
                                                                                                                                                                                                                                                 ccccccGCCGTATGCCCCGGCGACAACCCCGGATAAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCCCCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAcc  <  1:263508/237‑1 (MQ=255)
                                                                                                                                                                                                                                                   ||                                                                                                                                                                                                                                            
TAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCG‑‑‑‑‑GAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACC  >  NC_000913/2173127‑2173597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: