Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,216,209 | T→A | 13.7% | I90F (ATT→TTT) | yehW ← | putative ABC transporter permease |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,216,209 | 0 | T | A | 13.7% | 180.9 / 3.9 | 80 | I90F (ATT→TTT) | yehW | putative ABC transporter permease |
Reads supporting (aligned to +/- strand): ref base T (19/46); new base A (9/3); total (30/50) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.42e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGCCGCCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCAGCCAGAAAATCAGCGCCACAAACAGAGCAA > NC_000913/2215973‑2216442 | ggccgccCGCGGTGACCCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATa < 1:1026983/237‑1 (MQ=255) cgcctccAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATTCCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATa < 1:697303/237‑1 (MQ=255) ccAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACAGCGGCAAAACTTTc > 1:783693/1‑238 (MQ=255) aCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCCCTGCGACGGGCGGAAACGTCTGT‑CCAACGACGGCAAAAGGTTCCACCCAGGGGGG‑AAATTcc > 1:79778/1‑238 (MQ=255) aCGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCcg < 1:560763/239‑1 (MQ=255) tCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTTCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGCTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCACCACTGCGACGGCCGGAACAGCCGGT‑CCAACGGCGGCAACAGCTGTCACCAGAGGGGG‑CAAATCCGGGgccc > 2:1125787/1‑239 (MQ=255) tCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGCAAAGTCTGT‑CCAACGGAGGCAATAGGTTCCACCATGGGGCG‑AAAATCCGCGccc > 1:1034789/1‑238 (MQ=255) tCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCCAAAGGTTCCACCAGTGGGCG‑AAATTCCGGGcccc > 1:108079/1‑239 (MQ=255) cGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCACCCAAGGGGGG‑AAATTTCGGGCCCCACgggggggg > 1:874071/1‑234 (MQ=255) cGACTCATTCCCAACCCTTTCGCAACTTCTGTCACGGTGGAATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGt < 1:629931/239‑1 (MQ=255) aCTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCACTAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCCCGGGCGGGTGa > 1:395754/1‑239 (MQ=255) atccccATACCTTCCGCAACTTCGGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGc < 1:1019095/236‑1 (MQ=255) aTTCCCATGCCTTTCGCAACCTCTGTCACGCTGGCATCAAACGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGc < 1:145502/239‑1 (MQ=255) aTTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATCATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCACAAGCTTTCACCAGTGGGCG‑ACATTCCGCGCCCCACggggggcgggcaca > 2:429239/1‑228 (MQ=255) aTTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAAAAGTTTCCACCCATGGGGG‑AAATTCCACCCCCCCCGGGCGAGTGACCGc > 1:474405/1‑239 (MQ=255) aTTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGCGCGGAAAAGTCTGT‑CCAACGGCGGCAAAAGTTTCCCCCAGCGGGCG‑AAATTTCCCGCCCCCCGGGCGAGTGACAGc > 1:102439/1‑239 (MQ=255) tCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGGG‑AAATTTCGCGCCCCCCGCGCGGGGGACAGCaa > 1:349821/1‑239 (MQ=255) tCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAATGGGCG‑AAAATCCGCGCCCCACGGGCGAGGGGCAGCaa > 1:75455/1‑239 (MQ=255) tACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAATGGGGG‑AAATTCAGCGCCCCACCGGCGAGTGACAGCAATTCCg > 1:169389/1‑239 (MQ=255) tttCGCAACTTCTGTCCCGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCAc < 1:318160/239‑1 (MQ=255) tttCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCAc < 1:862949/239‑1 (MQ=255) tttCGCAACTTCTGGCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCAc < 1:951127/239‑1 (MQ=255) ttCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGACAAGTCTGT‑CCAACGGCGGCAATCGTTTCCACCCGTGGGCG‑AAATTCCGCGCCCCCCCGGCCAGTGACAGCCATTCCGGCAcc > 1:435892/1‑239 (MQ=255) ttCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCACAAGTTTCCACCAACGGGGG‑AAATTCCGCGCCCCCCGGGCGAGGGAAAGCAATTCCGGcccc > 1:339585/1‑239 (MQ=255) ttCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCAcc < 1:1224173/239‑1 (MQ=255) ttCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGGTTCCACCAATGGGCG‑AAATTTCGCCGCCCCCCGGCGGGGGACAGCAATTCCGGcccc > 1:420881/1‑239 (MQ=255) ttCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAACAGTTTCCACCAGTGGGCG‑GAATTCCGCGCCCCCCGGGCGAGCGACAGCAATTCCgggccc > 1:169107/1‑235 (MQ=255) ttCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGACTGT‑CCAACGGCGGCAATAGTTTCCACCAATGGGCG‑AAATTCCGCGCCCCCCCGGCGGGTGACAGCAATTCCCGCAcc > 1:150827/1‑239 (MQ=255) tCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAAAAGTTTCCCACACTGGGCGCAATTTCGGCGCCCCCCGGGCG‑GGGACACCAATTCCCGcccc > 1:452896/1‑238 (MQ=255) cccAACTTCTGTCACCGTGGCATCAAGAGCTTCCACGCCGGCAAGTGTCGCCTGCAGGGCGGGCAGCACAACGTAAAGGATCCAGGCGATAATCGCTTGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAg < 2:369718/237‑1 (MQ=255) acatctCTGACCCGGTGGCATCAACCGCCCCCAGCCAGGCAAGTGTCGCCGGAAGGGCGGGAAGCCCACGGTAAAGGATAAAGGCGAAAATCGCTGGTTGCAGACCAAAGCCGGTCACCGGAACGGCGATCGCCAGCCCTGCGACGGGCGGAAAAGGCTGT‑CCAGCGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTg < 2:58895/234‑1 (MQ=255) tGGCCTCCATCGCTCCCAGCCCGGCTAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGCTCAAGGCGATAAGCGCTGGTTGCAGACCAAAGCCGATCACCGGACCGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGca < 2:452896/239‑1 (MQ=255) aTCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAAATCCGCGCCCCCCGGGCGAGGGACAGAAATTCCGGCCCCAGGGCCAATAGTCCCCGcaaaca > 1:768016/1‑239 (MQ=255) aTCGCTCCCAGCCGGGCAAGTTTCGCCTGCAGGTCTGGCCGCACACCGTAAAGGATCAAGGCGATAATCGCTTGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACt < 2:390669/239‑1 (MQ=255) cGCTCCCAGGCCGGGAGTTGTCGCCTGCAGGACGGGCAGCACCCCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCGAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCg < 1:87761/239‑1 (MQ=255) gCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACggg < 1:1220786/179‑1 (MQ=255) gccGGGAAGTGTCGCCTGCAGGGCGGGCAGCACCCCGGAAAGGATCAAGGCGATAATCGCTGGTGGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCCGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCcca < 2:437285/238‑1 (MQ=255) ccctccaGTTTCCCCTGCAGGTCGGGTAGAGCACCGCAAAGGATCTAGGCGATAATCGGTGGTTGCAGACCAAAGCCGCTCACCGGAACGGCGATTGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATAACCGCAAACAAACTCGAAATTCccac < 2:107526/233‑1 (MQ=255) gCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGc > 1:513747/1‑194 (MQ=255) gggcgggcGGGCAGCACCCCGTAAAGGAGCAAGGGGATAAACGCTGGTTGCCGACCAAAGCCGATCCCCGGAGAGGCGATCGCCAGCCCGGAGACGGGCGGAAAAGGCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGc < 2:169389/232‑1 (MQ=255) gCAGGACGGGCAGCCCACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCCCCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGcc < 1:1228131/239‑1 (MQ=255) acCGTAAAGGATCAAGGCGATAAGCGCTGGTTGAAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCaa < 1:1202132/239‑1 (MQ=255) cGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGGGGGCG‑AAATACCGCTCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGGGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCCGCCAGAAATGGACCA‑GCGCCAGAGCTGCaaaa > 1:407500/1‑239 (MQ=255) aGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCCGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATAAGCCA‑GTGCCAAAGCTGCCAAAAtt > 1:377339/1‑237 (MQ=255) ttccaGGGGATAATCGCTGGTTGCCACCCAAGTCCTCGCCCCGGACCGGCCGACGCCAGCCCTGCGGCGGGCTGAAAAGCCTGT‑CCAACGGCGGCAATAGTTTCCCCCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCTGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCt < 2:874071/233‑1 (MQ=255) accAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCt < 1:175699/235‑1 (MQ=255) aTCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGGACGGCGATCGCCAGCACTTCGACGGCCGGAAAAGTATGT‑CCAACGGCGGCAAAAGTTTCCACCACTGGGCG‑AAATTTCCCACCCCCCGGGCGACGGACACCCACTCCGACACCCCCGCCACAGATCCCCGCCAACAAACTCGAAATACCCACCACCCCGCAAAGAGACACGTCCCACA‑CTGCAAAACTATCATg > 2:175699/1‑239 (MQ=255) cAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCCGAAATGAGCCA‑AGGCCCGAGCTGCAAAACTTTCtt > 1:1218946/1‑236 (MQ=255) cAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCt < 1:1125787/239‑1 (MQ=255) aGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCCCGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCCCAAACAAACCCGAAATTCCCACCAGCCCGAAATAAGCCA‑GTGCCCAAGCTGCCAAACTTTCTTGCTGa > 1:1170024/1‑239 (MQ=255) gttAATCGCTGTGTGCGGGACAAAGCGAGGCACCGGACCGTCATTCCCCAGCACGGGGACGGCTGGAAAAGTATGG‑CCAGCGGCGGGAATAGTTGCCCCCAGTGGGAG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCGCCGCAAACATACTCGAAATTCCCACCAGCCGGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATaaa < 2:872343/237‑1 (MQ=255) aTAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGCAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCCGAGCTGCAAAACTTTCTTGCTGGTAAAc > 1:393205/1‑239 (MQ=255) tAAGCGGTGGGTGCAGACCAAAGCCGATCACCGGAACGGCTATCGCCAGCACGGCGCCGGGCGGAAAAGGCTGT‑CCAACGGCGGCAAGAGGTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACGTTCTTGCTGATAAAc < 1:1239210/238‑1 (MQ=255) cTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAACCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGt > 1:928779/1‑239 (MQ=255) ggTTGGAAACCAAAGCCGATCCCCGGAACGGCGATCGGCCGCACTGGGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTgg < 2:393205/239‑1 (MQ=255) tgaccacacaaGCCGACTCCCGGAACGGCGATCCTCAGCGCTGCGACGGGCGGAAAAGTCTGT‑CCAACGGGGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAAGGACCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCa < 2:1077667/229‑1 (MQ=255) acccAAAGCCGATCACCGGAACGGCGACTCTCAGCACTGCGCCGGGGTGAAAAGTCGGT‑CCATCGGGGGCAATAGTTCACCTCAGGGGGCG‑AAATTCCGCGCCCCGCGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTGTCTCTCTGATAAACGGGTCGTGGCAGTtgt < 2:122492/237‑1 (MQ=255) ccAAAGGCTATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGG‑CCAACGGCGGCAATAGTTTCCAACAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTtgt < 1:928347/237‑1 (MQ=255) ccAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGC‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTtgt < 1:852815/237‑1 (MQ=255) ccGGCGATCCCCACCGCTGCGACGGGCGGAAAAGTCTGG‑CCAACGGCGGCAATAGGTTCCACCAGTGGGCG‑AAATTCCGCGACCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACa < 2:420881/237‑1 (MQ=255) tctccccAGCCCTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGACCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCggct < 2:395754/234‑1 (MQ=255) gttccgccgaccTGCCACGGGCGAAAAAGTCTTT‑CCAACGGCGGCAATAGTTCCCACCAGCTGGCC‑AAATTCCGCGCCCCCCGGGCGAGTGACAGCAATTCCGGCCACAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCggct < 2:108079/228‑1 (MQ=255) gctccccaccaCTGCCACGGTGCGAAAAGTCTGG‑ACGACGGGGGCAAACACTTCCCCCCGTGGGCT‑AAAATGCCGTCCCCAAGGGCGAGTGAAAGCAATGTCGGCACCCGTGCCAATGATCGCCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTATCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCggct < 2:349821/234‑1 (MQ=255) gATCGCCCGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCggc < 1:429239/238‑1 (MQ=255) caccggccgCACTGCCACGGGGGGAAAAGTCTGT‑CCAAGCGGGGCAATAGTTTCCACCAGTGGGCG‑AAAGTCCGCGCCCCACTGGCGGGTGCCAGCAATTCCGGCACCAGTGCCAATGATCCCCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAGCTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGAAAACAGCggct < 2:211778/231‑1 (MQ=255) ctgggcgggggCGGAAAA‑TCCGTGCCAACGGGGGAAATAGTTCCCCCCAGTGGCCG‑AAATACCGCGCCCCACGGGCGAGTGACAGCAATGCCGGCACCAGTGCCAAGGACCGCCGCAAACAAACTCGAAATTCCCACCATGCAGAAAGGAGCCG‑GTGCCAGAGTTGCAAAGCTTTCTTGCTGATAAAAGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGGAAgg < 2:169107/231‑1 (MQ=255) cctggcgcGGGCGGAAACGTTGGG‑CCAACGGCCGAAAATTGTTCCACCAGTGGGCA‑AAATGCCGCGACCCACGGGCGAGTGACCGCAATTCCGGCACCAGTGCAAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAgg < 2:150827/232‑1 (MQ=255) cgCGACGGGCGGAAAAGTCTGT‑CCCACGGCTGGAATAGTTTCACCCAGTGGTCG‑AAAGTCCGCACCCCCCGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCa < 2:541545/238‑1 (MQ=255) cGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGGCGTGGCAGTTGGGGGGACAAAGCAGCAAAAAGCGGCCGGCGGTaaagaag > 1:1066860/1‑233 (MQ=255) cGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCAGCCAg < 1:656842/239‑1 (MQ=255) gggCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCAGCCAg < 1:760984/238‑1 (MQ=255) cGGAAAAGTCTGT‑CCAACGGCGGCAATAATTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAATGCCAATGATCACCGCCAACCAACTCGAAATTCCCACCAACCAGAAAAGAACCA‑GTGCCCAAGCTGCCAAACCTTCTTGCTGATCAACCGGGAGTGGCAGTTGGGGGAACAACGCGGCAAAAACAGGCTGGCGGTAAAGGACCCAGaaaa > 2:309382/1‑239 (MQ=255) aaaGTGTGG‑CCGACGGAGGAAATATTTTCCACCAGTGGGCG‑AAAGTCCGCGCCACACGGGCGAGTGACAGCAATTCCGGCACCAGTGACAATGAGCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGGCAGAGCTGCAAAACTGTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGAAGCCAGAAAATCa < 2:435892/238‑1 (MQ=255) aaaGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCAGCCAGAAAATCa < 1:698047/238‑1 (MQ=255) aaaGTCCTT‑CCCACGGGGGAAAAATTTGCCACCAGTTGGCC‑AAATTCCGCGCCCCCCGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCAGCCAGAAAATCa < 2:407500/238‑1 (MQ=255) aaGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCAGCCAGAAAATCAgc < 1:266761/239‑1 (MQ=255) cTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAACCGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCAGCCAGAAAATCAGCGcc < 1:615024/238‑1 (MQ=255) aCGGCGGCAATTGTTTCCCCCCGGTGGCG‑AAATTCCGCCCCCCACGGGCGAATTACAGCAATTGCGGCACCAGTGACAATGATCACCTCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCAGCCAGAAAATCAGCGCCACAAACag < 2:75455/239‑1 (MQ=255) ggcCATAGTTTCCCCCCGTGGGCC‑AAATTCCGCGCCCAACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCAACAGCCAGAAATGGGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCAGCCAGAAAATCAGCGCCACAAACAGAGCaa < 2:928779/239‑1 (MQ=255) gcAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCAGCCAGAAAATCAGCGCCACAAACAGAGCaa < 1:986259/238‑1 (MQ=255) | AGCCGCCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGT‑CCAACGGCGGCAATAGTTTCCACCAGTGGGCG‑AAATTCCGCGCCCCACGGGCGAGTGACAGCAATTCCGGCACCAGTGCCAATGATCACCGCAAACAAACTCGAAATTCCCACCAGCCAGAAATGAGCCA‑GTGCCAGAGCTGCAAAACTTTCTTGCTGATAAACGGGTCGTGGCAGTTGTGGGAACAAGGCAGCAAACAGCGGCTGGCTGTAAGGCAGCCAGAAAATCAGCGCCACAAACAGAGCAA > NC_000913/2215973‑2216442 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |