BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,251,685	A→G	11.6%	E221G (GAG→GGG)	yqjG →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,251,685	0	A	G	11.6%	278.0 / 4.7	107	E221G (GAG→GGG)	yqjG	putative S‑transferase
Reads supporting (aligned to +/- strand): ref base A (27/66); new base G (11/3); total (38/69)
Fisher's exact test for biased strand distribution p-value = 5.74e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

ACCATCGTCAGCAACGAATCAGCAGAAATCATCCGCATGTTCAATACCGCGTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCATATCCGTAATCATTACTTCCGC  >  NC_000913/3251450‑3251917

cccAGCGTCAGCAAAGAATCAGCAGAAATCATCCGCATGTTCAATACCGGGGTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCa                                                                                                                                                                                                                                        <  1:418918/238‑1 (MQ=255)

    tCGTCAGCAACGAATCAGCAGAAATCATCCGCATGTTCAATACCGCGTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGGGTTTGAAAC‑CTTGGCACGGATGGGGCCGAtt                                                                                                                                                                                                                                    >  1:1214676/1‑239 (MQ=255)

     cctCAGCAACGAATCAGGAGAAATCCTCCGCATGTTCAATGCCGCGTTTGATGCGCTGGGCGGGAAAGCTGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGAttt                                                                                                                                                                                                                                   <  1:93503/237‑1 (MQ=255)

     cGTCAGCAACGAATCAGCAGAAATCATCCGCATGTTCAATACCGCGTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGAttt                                                                                                                                                                                                                                   <  1:1041034/239‑1 (MQ=255)

         aGCAACGAATCAGCAGAAATCATCCGCATGTTCAATACCGCGTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGCCACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTAGCGGCAGGGTTTGAATC‑GATGGCACGACTGGAGCAGATTTTAg                                                                                                                                                                                                                                >  1:137887/1‑238 (MQ=255)

         aGCAACGAATCAGCAGAAATCATCCGCATGTTCAATACCGCGTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGGGCCGATTTTAg                                                                                                                                                                                                                                >  1:864041/1‑238 (MQ=255)

          gcaaagatgcagcagAAATGTCCCGCCTTTTCAATCCCGGCTTTGATGCCGTTGGCGGCAAAGCGGGTGATTACTTCCCGCCCGGCCTGCAAGCAAAAATTGACAAACTTAACGGCTGGAGTTAGGACGCCTGTAACAACGGCTGGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTGACGACGA‑GGAGGTGGCGAAAGTGTTTGAATC‑GCTGACACGACTGGAGCAGATTTTAGGc                                                                                                                                                                                                                              <  2:24323/230‑1 (MQ=255)

          gCAACGAATCAGCAGAAATCGTCCGCATGTTCAATCCCGCGATTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGc                                                                                                                                                                                                                              <  1:555212/239‑1 (MQ=255)

            aaCGAAACAGCAGAAATCCTCCGCATGTTCAATTCCGCGTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAgg                                                                                                                                                                                                                               <  1:1166973/236‑1 (MQ=255)

                                ccGCATGTTCAATACCGCGTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGGGTTTTAAGC‑CCTGGCAAGACTGGAGAAGATTTTAGGCCACCATCGTTACCTGAccctga                                                                                                                                                                                                        >  1:860090/1‑235 (MQ=255)

                                   cATGTTCAATACCGCGTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCCCCAGCCAGGAAGCTTACGACGA‑GGCGGGAGCGAAAGAGTTTGAATC‑GCTGGCACGAATGGGGCAGATTTTAGGCCAGCATCGTTAACTTACCGGCAAc                                                                                                                                                                                                      >  1:871160/1‑238 (MQ=255)

                                     tGTTCAATACCGCGTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGGCGAGGGCGGAGG‑GCAAGGGTGTGAAACTCCGGGCCAGCCGGGGGCAGTTTTTGGGCACGCATCTTTACCAAACCGGCAAca                                                                                                                                                                                                     >  2:6589/1‑237 (MQ=255)

                                     tGTTCAATACCGCGTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGGAAGTGTTTGAATC‑GCTGGACCGACTGGAg                                                                                                                                                                                                                                          >  1:215163/1‑200 (MQ=255)

                                                gcgTTTGATGGGCTGGGCGCGAAAGCGGGTTATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGAATCCGTTAACAACGGCGTGTAAAAAGCTGGTTTTGCCACCAGCCAGGAAGGTTACGACGA‑GGTGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGc                                                                                                                                                                                        <  1:504622/239‑1 (MQ=255)

                                                gcgTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGc                                                                                                                                                                                        <  1:1246174/239‑1 (MQ=255)

                                                 cgTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGGGGAAGGGTTTGAATC‑GCTGGGCCGACTTGGGACGAATTTAGGCCAACATCGTTACCTGACCCGCAACCAGCTAACCGaaac                                                                                                                                                                                        >  1:386413/1‑236 (MQ=255)

                                                 cgTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGGAAGTGTTTGAATC‑GCTGGCACGGCTGGGGCAGATTTTAGGCCAGCATCGTTTACTGACCGGCAACCAGCTAACCGAAGcc                                                                                                                                                                                       >  1:1037840/1‑239 (MQ=255)

                                                 cgTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGGGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGcc                                                                                                                                                                                       >  1:401045/1‑239 (MQ=255)

                                                 cgTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGGGCAGATTTTAGGCCAGCATAGTTACCTGACCGGCAACCAGCCAACCGAAAcc                                                                                                                                                                                       >  1:1040645/1‑239 (MQ=255)

                                                 cgTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGGCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAAcc                                                                                                                                                                                       >  1:376228/1‑239 (MQ=255)

                                                 cgTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGGGTTTGAATC‑CCTGGCCCGACTGGGGCAGATTTTAGGCCAGCATCGTTACCTGACCCGCAACCAGCTAACCGAAAcc                                                                                                                                                                                       >  1:303757/1‑239 (MQ=255)

                                                       aggcgcTTGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGAATTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATAtt                                                                                                                                                                                 <  1:1242337/237‑1 (MQ=255)

                                                        tGCGCTGGGCGGGAAAGGGGGTGATTTCGACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATAtt                                                                                                                                                                                 <  1:1028082/238‑1 (MQ=255)

                                                          cgcTGGGCCGCAAAGCGGGTTATTACTACCCCCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGc                                                                                                                                                                              <  1:529601/239‑1 (MQ=255)

                                                           gcTGGGGGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGGTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGGCGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGc                                                                                                                                                                              <  1:1015973/238‑1 (MQ=255)

                                                            cTGGGGGCGAAAGCGGGTGTTTACAACCCACCAGCCCTGCAAACAAAAATTGACGAACTAAACGGGTGAATTTATGACACCTTTAACAACGGGGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCt                                                                                                                                                                            <  2:684452/239‑1 (MQ=255)

                                                             tGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCt                                                                                                                                                                            <  1:6589/238‑1 (MQ=255)

                                                                  gcgAAAGCGGGTGATTAATTACCTCCAGCCCTGCAAAAAAAAATTGACGAACTTCACGGCGGGATTTAAGAGCCCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCGCCAGCCAGGAAGCTTCCGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTTGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACAAGCTAACCGAAGCCGATATTCGCCTGTGGa                                                                                                                                                                       <  2:906843/238‑1 (MQ=255)

                                                                           ggTTATTAATTCCCCCCCACCCTGCAAACAAAAATTGACGAACTTAACGGGTGGGTTTATGACCCCGTTAACAACGGGGTTTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGGCGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGt                                                                                                                                                            <  2:864041/239‑1 (MQ=255)

                                                                            ttGATTCCTCCCCCCCAGCCCTGCAAAAAAAAATTGACGAACTGAACGGCTGGGTTTAGGCCCCCGTTAACAGCGGCGTGTATAAAGCGGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAAGC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGt                                                                                                                                                            <  2:561002/237‑1 (MQ=255)

                                                                                     tcccTCCAGCCTTGCAAACAAAATTTGACGAACTGAACGGCGGGATTTATGACACCGTTAGCAACGGGGTGTATAAAGGTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGGTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGAt                                                                                                                                                  <  2:542588/238‑1 (MQ=255)

                                                                                     cccccccCGCCCCGAAAACAAAAATTGTCAAAATTAAGGGCGGGATTTATGGCACCGTTAACACCGGCGTGTATAAAGCGGGTTTTGCCACCAGGCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTGCCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGAt                                                                                                                                                  <  2:1088033/238‑1 (MQ=255)

                                                                                     acCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGAt                                                                                                                                                  <  1:1075591/239‑1 (MQ=255)

                                                                                      cccTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCCGCTAACCGCAGCCGAAATTCGCCCGTGGGCAACCCT‑GGTGCGgtttttt                                                                                                                                                  >  1:542588/1‑235 (MQ=255)

                                                                                      cccTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATc                                                                                                                                                 <  1:749999/239‑1 (MQ=255)

                                                                                          cccccccgtaaaacaaaAATGGCACAACGTAACTGTTTGATTTATGACACCGTTAACAACGGCTTGTTTAAAGCTGGTTGTGCCACCAGCCCGGAATCTGACGACAC‑GGCTGTGGCCAAAGTGTTTGAATC‑GCGGGCCCGACGGGAGCAGATTTGAGGCCCGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTTGACTACGCT‑GGTGCGTTCTGATCCGgt                                                                                                                                             <  2:1264935/229‑1 (MQ=255)

                                                                                           cAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGGGTTTGAATC‑CCTTGCACCGTCGGAGCAAGTTTTAAGCCAGCATCGTTACCCTACCGGCGACCAACAAAACCAAGCCCATAATTGCCCGTGGGATAACCT‑GGGGAGGTTTTATCCCgtg                                                                                                                                            >  2:281714/1‑239 (MQ=255)

                                                                                              cccTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTTGCCTTTGGACTACCCT‑TGTGCGGTTTTATCCgggggat                                                                                                                                         >  1:721841/1‑234 (MQ=255)

                                                                                                cTGCAAAAAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTAtgt                                                                                                                                       <  1:524198/239‑1 (MQ=255)

                                                                                                 tGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGa                                                                                                                                                                                           >  1:1242317/1‑187 (MQ=255)

                                                                                                 tGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCATGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGa                                                                                                                                                                                           <  2:1242317/187‑1 (MQ=255)

                                                                                                  gccaacaaaAGTTTGCGAATTTAAAGGCTGGGTTTAAGTCCCCGTTAACCACGGCGTTGATAAAGCTTGTTTTGACACCAGCCAGGAAGGTTACGACGG‑GGCGGGTGCGAAAGTGTTTGAATC‑GCTGGCACGACTTGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGa                                                                                                                                     <  2:1191050/236‑1 (MQ=255)

                                                                                                  ccaaaaaaaAATGGACGATCTTTACGTTTGGATTTTTGACCCCCTTAAAAACGGCGTGTATAAGGCTGGTTTTGCGACCAGGCGGGAAGCTTAGGACGA‑GGCGGTGGCGGAAGGGTTTGAAGC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTTCCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGGTTTGATCCGGTGTATGTGa                                                                                                                                     <  2:1236367/238‑1 (MQ=255)

                                                                                                   gaaacaaaAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTATATGTGAc                                                                                                                                    <  1:448620/238‑1 (MQ=255)

                                                                                                    aaacaaaAATTGTCGAACTTAACGGTTGGATTTAGGACACCGTTAACAACGGCGTGTAAAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGGGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGAcc                                                                                                                                   <  2:376228/239‑1 (MQ=255)

                                                                                                    aaacaaaAATTGACGAACTTAACGGCTGGATTTATGACCCCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGAcc                                                                                                                                   <  1:348300/239‑1 (MQ=255)

                                                                                                    aaacaaaAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGAc                                                                                                                                    <  1:1283822/238‑1 (MQ=255)

                                                                                                        aaaaaTTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGGTATTCCCCTTTGGACTACGCT‑CGTGCGTTTTGATCCGGTGTATGTGACCCACt                                                                                                                               >  1:841893/1‑239 (MQ=255)

                                                                                                            aTTGACGAACTTAACGGCTGGATTTATGACCCCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATc                                                                                                                                                 <  1:151843/217‑1 (MQ=255)

                                                                                                            aTTGACGAACTTAACGGCTGGATTTATGACCCCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCaa                                                                                                                           <  1:1056910/239‑1 (MQ=255)

                                                                                                              tGACGAACTTTACGGTTGGATTTATGACCCCGTTAACAACGGCGTGTATAAAGCGGGTTTTGCCCCCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAgt                                                                                                                         <  2:195407/239‑1 (MQ=255)

                                                                                                              tGACGAACTTAACGGCTGGATTTATGACCCCGTTAACAACGGCGTGGATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAgt                                                                                                                         <  1:1285834/239‑1 (MQ=255)

                                                                                                                         aCGGCTGTATTTATTAACCCGTTAACAACGGGGTGTATAAAGTTGGTTTGGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCAAGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATTTGACCCACTTCAAGTGTGATAAGCAc                                                                                                              <  2:27350/239‑1 (MQ=255)

                                                                                                                         aCGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCAc                                                                                                              <  1:627833/239‑1 (MQ=255)

                                                                                                                                taTTTTTGGCCCCGTTAACAAGGGGTGGTATAAAGGTGGTTTTGCCACCCGCAAGGAAGCTTACGACGA‑GGGGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCa                                                                                                       <  2:66175/238‑1 (MQ=255)

                                                                                                                                 aTTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCAc                                                                                                              <  1:184854/231‑1 (MQ=255)

                                                                                                                                    ttttgccccGTTAACACCGGGGTGTATAAAGCTGGTTTTGCCCCCAGCCAGGAAGCTTACGACGG‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGa                                                                                                   <  2:721841/232‑1 (MQ=255)

                                                                                                                                    tatgcacccGTTAACAACGGGGTGTATAAAGCTGGTTTGGCCCCCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGa                                                                                                   <  2:789685/232‑1 (MQ=255)

                                                                                                                                          acCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGGGGTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCACCCTTACCTGACCGGGCACCAGCTAACCGAAGCCGATATTCGCCCGTGGGCAACGCTCGGCGGGTTTTG‑TCCGGGGTGTGTGAACCCCTTTAAAGATGATAAGACCCGCCTCAGCGGTTACCt                                                                                             >  2:931477/1‑239 (MQ=255)

                                                                                                                                           ccGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGGGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGAAATTCGCCTGTGGACTACGCT‑GGTGCGGTTTGATCCGGGGGATGGGACCCACTTCAAAGGGGATAAGCCCCCCATCAGCGATTACCtt                                                                                            >  1:907049/1‑238 (MQ=255)

                                                                                                                                            cGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTTACCGGCAACCAGCTAACCGAAACCGGTATTCGCCTGTGGACTACGCT‑GGGGCGTTTTTATCCGGGGGATGTGACCCCATTCCAATGGTATAAACCCCCCCTCAGCGATTAcccta                                                                                           >  1:789685/1‑236 (MQ=255)

                                                                                                                                            cGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCg                                                                                                                                               <  1:830232/187‑1 (MQ=255)

                                                                                                                                              ttaaaacaggCGTTTTTTAAGCGGGTTTTGCCACCAGCCAGGAAGCTTGCGTAGA‑TGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTTCCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAAt                                                                                         <  2:710207/231‑1 (MQ=255)

                                                                                                                                              ttAACAACGGCGTTGATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAAt                                                                                         <  1:723699/239‑1 (MQ=255)

                                                                                                                                              ttAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTTCCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGGCTAAGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCCCTTCCAATGTGAAAAGGACCGCCTAAGCGATTAACTTAAt                                                                                         >  1:740523/1‑239 (MQ=255)

                                                                                                                                                    acGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTAc                                                                                   <  1:38498/239‑1 (MQ=255)

                                                                                                                                                    acGGCGGGTATAAAGCTGGTTTGGCCACCAGCCAGGAAGCTGACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTAc                                                                                   <  1:894749/239‑1 (MQ=255)

                                                                                                                                                      ggCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGAGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGAACGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCCGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACgg                                                                                 >  1:562887/1‑239 (MQ=255)

                                                                                                                                                        cGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGGAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCCACCAGCTAACCGCAGGCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGGATGTGCCCCCCTTCAAGGGTGATAAGCACCGCCTCAGCGGTTACCGGAATCTgagcgggt                                                                               >  2:905508/1‑232 (MQ=255)

                                                                                                                                                               aaaGGTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTgcg                                                                        <  1:483575/239‑1 (MQ=255)

                                                                                                                                                               aaaGCTTGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTgcg                                                                        <  1:274653/239‑1 (MQ=255)

                                                                                                                                                                 atttGGTTTTGCCACCACCCAGGTAGGTTACGGCGG‑TGCGGTGGCGAAAGTGTTTTAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCCGCATCGGTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGcgcg                                                                      <  2:401045/236‑1 (MQ=255)

                                                                                                                                                                 atcTGGTTTTGCCCCCAGCCAGGAAGCTTACGACGG‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCCGCATCGTTGCCTGACCGGCAACCAGCTAACCGAAGCCGATAGTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGcgcg                                                                      <  2:334142/237‑1 (MQ=255)

                                                                                                                                                                 aGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGCTAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTTTGcgcg                                                                      >  1:254610/1‑239 (MQ=255)

                                                                                                                                                                    tgttttgGCCACCAGGCAGGAAGCTTACGAGGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGat                                                                    <  2:841893/235‑1 (MQ=255)

                                                                                                                                                                      gTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATTCGGTGTATGGGACCCCCTTCCAGTGTGATAAGCACCGCACCAGCGCTTACCTGAATCTTTACGGGTTTTTTCGCGcta                                                                   >  1:7496/1‑237 (MQ=255)

                                                                                                                                                                      gTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGGACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTTGGCGata                                                                   >  1:1038475/1‑237 (MQ=255)

                                                                                                                                                                      gTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGGGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGGGata                                                                   >  1:49692/1‑237 (MQ=255)

                                                                                                                                                                      gTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGAGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAACCGATTTTAGGCCAGCATCGTTACCTCACCGGCAACCAGCTCACCGAAGCCGGTATTCGCCTGTGGACTACGCT‑GGGGCCTTTTTATCCCGGGTATGTGTCCCCCTTCAAATGGGATTAGCCACGCATCAACGATTACCTTCATCTTTACGGtttttttcggggatc                                                                  >  2:194875/1‑227 (MQ=255)

                                                                                                                                                                                     cggAATCTTTCGTCGG‑GGGGGGTGCGAAATTTTTTGAAAC‑GGTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCgggg                                                  <  2:1271267/238‑1 (MQ=255)

                                                                                                                                                                                          gCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGGGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTTTACGGTTTTCTGCGCGGTTTCTACCCAATGCCGGGGATCg                                              >  1:1294234/1‑238 (MQ=255)

                                                                                                                                                                                             tACGACGA‑GGCGGTGGCGAAAGGGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGAACCGCATCAGCGATTACCTGAATCTGGCCGGCTTTTTGCCCGAAATCTACCAGATGCCGGGGATCGCCGa                                          >  1:1035035/1‑239 (MQ=255)

                                                                                                                                                                                              acggcgG‑GGCGGTGGCCAAAGGTTTTGAAGC‑CCTGGCCCGACCGGGACAGATTTGAGGCCGGCAGCGGTACCTGACCGGCAACCAGCTAACCGAAGGCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGaa                                         <  2:263509/239‑1 (MQ=255)

                                                                                                                                                                                                 acga‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGGTTTCTGCGCGATATCTACCAGATGCCGGGGAGCGCCGGAACa                                      >  1:263509/1‑239 (MQ=255)

                                                                                                                                                                                                    a‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTc                                   >  1:730057/1‑239 (MQ=255)

                                                                                                                                                                                                      ggCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTTTACGGTTTTTTGCCCGATATCTACCCAATGCCGGGGATCGCCGAAACAGTCa                                  >  1:61082/1‑239 (MQ=255)

                                                                                                                                                                                                      ggCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGGGTATGTGACCCACTTCAAATGTGATAAGCACCGCATCAGCGGTTACCTGAATCTGTACGGTTTTCTGGGCGATTTCTACCAGATCCCGGGGATGGCCCAAAACGTCa                                  >  1:1032308/1‑239 (MQ=255)

                                                                                                                                                                                                         ggTGGCGAAAGTTTTTGAATC‑CCTGGCACGACTGGAGAAGATTTTAGGCCTGGATCGTGTCCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAAt                                <  2:1040645/238‑1 (MQ=255)

                                                                                                                                                                                                               gAAAGTGTTTTAATC‑CCTGGCCCGACTGGAGGAGTTTTTAGGCCAGCACCGTTACCGGCCCGGCAACCAGCTAACCTAAGCCGATAGTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGAt                          <  2:386413/238‑1 (MQ=255)

                                                                                                                                                                                                               gAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGAt                          <  1:1094449/238‑1 (MQ=255)

                                                                                                                                                                                                                 aaGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCGTCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCa                        <  1:1022276/238‑1 (MQ=255)

                                                                                                                                                                                                                      tttGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCATATCCg                  <  1:209799/239‑1 (MQ=255)

                                                                                                                                                                                                                        tGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGTCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCATATCCGTa                <  1:905508/239‑1 (MQ=255)

                                                                                                                                                                                                                        tGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCATATCCGTa                >  1:1138460/1‑239 (MQ=255)

                                                                                                                                                                                                                         ttaatccgtGGCACTGCTGGAGGAGTTTTTAGGCCAGGATTGTTACACGGCCGGCATCCAGCTAACCGAAGTCGATATTCGCCTGTGGACTACGCT‑GGTTCGTTTTGATCCGGTGTATGTGAACCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCATATCCGTa                <  2:1035035/231‑1 (MQ=255)

                                                                                                                                                                                                                               gCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATTTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCATATCCGAAATCAt           >  1:1008834/1‑238 (MQ=255)

                                                                                                                                                                                                                                     aCGACTGGAGCAGATTTTAGGCCAGATTCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTGGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCATATCCGTAATCATTACTTcc    <  1:608386/239‑1 (MQ=255)

                                                                                                                                                                                                                                       gACTGGGGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCATATCCGTAATCATTACTTCCGc  <  1:723593/239‑1 (MQ=255)

                                                                                                                                                                                                                                       gACTGGAGCAGATTTTAGGCCAGCATCGTTGCCTGCCCGGCAACCAGCTACCCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGAAAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCATATCCGTAATCATTACTTCCGc  <  1:664010/239‑1 (MQ=255)

                                                                                                                                                                                                                                       gACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCATATCCGTAATCATTACTTCCGc  <  1:931477/239‑1 (MQ=255)

ACCATCGTCAGCAACGAATCAGCAGAAATCATCCGCATGTTCAATACCGCGTTTGATGCGCTGGGCGCGAAAGCGGGTGATTACTACCCTCCAGCCCTGCAAACAAAAATTGACGAACTTAACGGCTGGATTTATGACACCGTTAACAACGGCGTGTATAAAGCTGGTTTTGCCACCAGCCAGGAAGCTTACGACGA‑GGCGGTGGCGAAAGTGTTTGAATC‑GCTGGCACGACTGGAGCAGATTTTAGGCCAGCATCGTTACCTGACCGGCAACCAGCTAACCGAAGCCGATATTCGCCTGTGGACTACGCT‑GGTGCGTTTTGATCCGGTGTATGTGACCCACTTCAAGTGTGATAAGCACCGCATCAGCGATTACCTGAATCTGTACGGTTTTCTGCGCGATATCTACCAGATGCCGGGGATCGCCGAAACAGTCAATTTCGATCATATCCGTAATCATTACTTCCGC  >  NC_000913/3251450‑3251917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`