Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,757,532 G→A 15.7% S99F (TCT→TTT)  yiaY ← L‑threonine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,757,5320GA15.7% 110.8 / 2.0 48S99F (TCT→TTT) yiaYL‑threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (35/5);  new base A (2/6);  total (37/11)
Fisher's exact test for biased strand distribution p-value = 8.48e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCAT  >  NC_000913/3757299‑3757761
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       
tgTCATAGACAGAAAGGAGGGGGTTGACATTTTAAAAAAAACGCGACATTTGTATGGGACGCGTTGCGTAAGTGATGATGCAAAAGGGGTTCTTTTCAGGGGCCGTCCCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAacc                                                                                                                                                                                                                                     <  1:1145685/237‑1 (MQ=255)
agTCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCATGCCCATACCTTTTGGCCAGCCGTGGGGGGAacc                                                                                                                                                                                                                                     >  1:1185605/1‑238 (MQ=255)
agTCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCCCCCTTGGCTGCCACCAGCGCAATACCTTTTGCCCAGTCGTGTGGAGAacc                                                                                                                                                                                                                                     >  1:486526/1‑238 (MQ=255)
agTCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTCCGGCTTTGACAG‑GCGGTCAACGCCTTCGTAATCCGGAATATCACCGCCATTGGCTGCGCCCATCCCAAAACCTTTTGCACAGCAGTGTGGAAAaca                                                                                                                                                                                                                                     >  2:138554/1‑237 (MQ=255)
  tCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCCCCACCGCAATACCTTTTGCGCAGTCGTGTGGAGAaccac                                                                                                                                                                                                                                   >  1:600087/1‑238 (MQ=255)
  tCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGAGGTTTTG‑CAGAGCGGTCAACGCCCTCGTAATCGCGAATATCGCCGCCCTTTCGTGCCCCCAGCGCAATACCTTTTGCGCAAGCGCGTGGAGGaccac                                                                                                                                                                                                                                   >  1:932679/1‑238 (MQ=255)
          aGAAAGCAGCGAAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGACGTGTGGGGAACCACCGCC‑CAAgg                                                                                                                                                                                                                         >  1:906180/1‑239 (MQ=255)
              agcagcGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGGGAACCACCGCC‑TAAGgaga                                                                                                                                                                                                                      >  1:395147/1‑238 (MQ=255)
                    ggAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCCCCATTGGCTGCCACCAGCCCAATACCTTTTTCGCAGTCGTGTGGGAGACCACCGCC‑TAAGGAGATCACGCt                                                                                                                                                                                                               >  1:582421/1‑239 (MQ=255)
                      aGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCCATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCtt                                                                                                                                                                                                              >  1:1106262/1‑237 (MQ=255)
                       gTGACTTTTTTAACAACAACCGCTGTTGTGATAGGCCGCGCTTCGTCAGTGAGGGTGGAAAAGCGGTTTTTTACAGGGGCCTACCCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTAt                                                                                                                                                                                                             <  1:516877/238‑1 (MQ=255)
                                          tccgctttttGATAGGCGGCGCTTCGTCAGGTAGTATGAAGAAACGGTTAATTTCGGGGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGt                                                                                                                                                                                        <  1:1277402/233‑1 (MQ=255)
                                          tCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCCCCAGCGCAATACCTTTTGCGCAGTCGTGGGGAGAACCACCGCC‑TAAGGAGATCACGCTATCCCA‑ATTATTCTCTTTAAGt                                                                                                                                                                                        >  1:1170260/1‑239 (MQ=255)
                                                        tGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCCCGCTATCGCA‑CTTATTCTCTTTAAGTAATTTCAAACCTgc                                                                                                                                                                          >  1:1275056/1‑239 (MQ=255)
                                                           cgcgCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTgcggc                                                                                                                                                                       >  1:1200775/1‑239 (MQ=255)
                                                               cTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATTCCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTGTTGCGCAGTCGTGTGGAGAACCCCCGCC‑AAAGGGGATCACACCATCGCACATTTTCCTTTTTAAG‑AATTTAAAACCTagggcggag                                                                                                                                                                   >  2:1080883/1‑236 (MQ=255)
                                                               cTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTTTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCC‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGGGACg                                                                                                                                                                   >  1:18479/1‑239 (MQ=255)
                                                                  cGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCCCCGCC‑TAAGGAGATCACCACATCCCA‑ATTATTCTCTTTAAGGAATTTCAAACCTGCGGGGACGttt                                                                                                                                                                >  1:1114431/1‑239 (MQ=255)
                                                                          atgatgCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCgtggt                                                                                                                                                        >  1:702732/1‑239 (MQ=255)
                                                                             atgCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCCCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTggg                                                                                                                                                     >  1:784136/1‑239 (MQ=255)
                                                                                     aCGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGtt                                                                                                                                             >  1:858077/1‑239 (MQ=255)
                                                                                               tCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAg                                                                                                                                                                                                                          >  1:809598/1‑153 (MQ=255)
                                                                                                                  tGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAata                                                                                                                >  1:1171860/1‑239 (MQ=255)
                                                                                                                            ggCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGttct                                                                                                       >  1:959910/1‑238 (MQ=255)
                                                                                                                                           cTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTTCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTTAAAAATTTTCGTTCTTCCAGTGCTTTTTGc                                                                                        >  1:1298481/1‑238 (MQ=255)
                                                                                                                                              gggTTTTG‑CAGGGGGGGCAACGTCTTGATAACGGGAAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGAGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGcaca                                                                                     <  1:12342/237‑1 (MQ=255)
                                                                                                                                                            cGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATAcc                                                                       >  1:1047308/1‑239 (MQ=255)
                                                                                                                                                            cGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGCAATTTCAAACCTGCGGCGACGTTTTCCGTGGCGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCCCATAGCCCGCCATAcc                                                                       >  1:328529/1‑239 (MQ=255)
                                                                                                                                                                      cTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCCGTGCCTTTTGCACATCGCCCGCCATACCCAATTTct                                                               >  1:90947/1‑236 (MQ=255)
                                                                                                                                                                      cTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGGGCCATCCTAAATAACGCTAAAAATATTGCTTTCTTCCAGGGCTTTTTGCACATCTCCCGCCATAACTAATTTCGtt                                                             >  1:851607/1‑239 (MQ=255)
                                                                                                                                                                             aTCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACatat                                                      >  1:241500/1‑239 (MQ=255)
                                                                                                                                                                              tCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCCTTTGCGCAGTCGTGTGAAGAACCACCGCC‑TAAGGAGATCACGCTATTGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTTCGGCGACGTTTTCCTTGGTGGGTCTCGGTAGGGTGCCATCATAAATAACGTTAAAAAAATTGCGTTCTTCTAGTGCTTTTTGTACTTAGCCGCACATACCTAATTTTGTaaaattata                                                     >  2:1263247/1‑233 (MQ=255)
                                                                                                                                                                                           gcccATTGGTTGCCCCCAGCGCAATCCCTTTTGCGCATTCGTGTGGAAACCCCCCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCGGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGTTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAAtt                                        <  1:721156/237‑1 (MQ=255)
                                                                                                                                                                                                gtGGCGGCCACCCGCGCAATCCCTTGTGCGCTGTTGTGTGGAAACCCCCCGCC‑AAAGGAGATCACGTTATCGAA‑ATTATTCCTTTTAAGTATTGTCAACCCGGCGGCGACGTTTTCCGGGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGt                                   <  1:672703/238‑1 (MQ=255)
                                                                                                                                                                                                        caccaGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTaa                           >  1:592419/1‑239 (MQ=255)
                                                                                                                                                                                                            aGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAACATATTGCGTTCTTCCAGGGCTTTTTTCACATCGCCCGCCAAAACTAATTTCGTTAACCAATGTTCAGTGACAATAAAGGTACGGGAAAAAcc                       >  2:535082/1‑239 (MQ=255)
                                                                                                                                                                                                                  aTACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAAt                  >  1:652667/1‑238 (MQ=255)
                                                                                                                                                                                                                    cccTTTTGCGAAGTCGTGTGGAAACCCCCCCCC‑TAAGGAGTTCACCCTATCGCA‑ATTATTCTTTTTAAGTAATTCCAAACCTGCGGCGACGTTTCCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATTTTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTg               <  1:197255/238‑1 (MQ=255)
                                                                                                                                                                                                                     cttttttccgtgTCGTGGGGGAAACCCCCCCCTTAAGGGTATCCCGTTATCGCA‑TTTATTCTCTTTAAGTAATGACAACCCGGGGGCACCGTTTTCGGTGGGGGGTTTAGGTTGGGGTCATTCATAAATAACGATAAAATTTTTGCTTTCTTCCAGTGCTTTTTGCCCATCTCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTg               <  2:959910/228‑1 (MQ=255)
                                                                                                                                                                                                                        tttGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCcat           >  1:206684/1‑239 (MQ=255)
                                                                                                                                                                                                                             gcAGTCGGTGGGAGAACCCCCTCC‑AAAGGAGATCACGCTATCGCA‑TTTATTCTCTTTAAGTAATTTCAACCCTGCGGCGACGTTTTCCGTGGTGGGTTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCata       <  1:753528/238‑1 (MQ=255)
                                                                                                                                                                                                                               aGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAg                                                >  1:409985/1‑195 (MQ=255)
                                                                                                                                                                                                                                ggcggggggAAAACCACCGCT‑GAGGGAGATCCCGTTATCGCA‑ATTATTCTCTTAAAGTAATTCCAACCCGGCGGCGACGTTTCCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCa   <  1:249176/232‑1 (MQ=255)
                                                                                                                                                                                                                                ggcGTGGGGAAACCCCCCGAC‑TAAGGAGATCAGGTTTTCGAA‑ATTTTTCTCTTAAAGTAATTTAAAACCTGGGGCGACGTTTTCCGTGGTGGGGTTAGTTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCa   <  1:138554/237‑1 (MQ=255)
                                                                                                                                                                                                                                gTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTCACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCAAAATTTGCcaatacattaa   >  2:254644/1‑230 (MQ=255)
                                                                                                                                                                                                                                gTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGAGCTTTTTGCACATCGCCCTCCAACCCTAATTTCGTTAACATATTTTCATCGGCAATTAAGGTACGGTGAAAACCATAAACTGAcataacataaa   >  2:677542/1‑231 (MQ=255)
                                                                                                                                                                                                                                 tCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATTTTGTCAGTGACAATTCAGGAACCGGTAAACCCAAAATATCCCATCATAATCAt  >  2:310368/1‑239 (MQ=255)
                                                                                                                                                                                                                                 tCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATATGCCATCATATTCAt  >  1:40324/1‑239 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                       
AGTCATTGACAGAAAGCAGCGGAGTGACATGTTTATCAACAATCGCCATTTTGATATGACGCGCTTCGTCAGTGATGATGCAGAAACGGGTCATTTCAGAGGCCGTACCCGCCGTGGTATTGATGGCGATCATCGGCAGCTGCGGTTTTG‑CAGAGCGGTCAACGCCTTCGTAATCGCGAATATCGCCGCCATTGGCTGCCACCAGCGCAATACCTTTTGCGCAGTCGTGTGGAGAACCACCGCC‑TAAGGAGATCACGCTATCGCA‑ATTATTCTCTTTAAGTAATTTCAAACCTGCGGCGACGTTTTCCGTGGTGGGGTTAGGTTGGGTGCCATCATAAATAACGCTAAAAATATTGCGTTCTTCCAGTGCTTTTTGCACATCGCCCGCCATACCTAATTTCGTTAACATATTGTCAGTGACAATTAAGGTACGGGTAAATCCATAATCTGCCATCATATTCAT  >  NC_000913/3757299‑3757761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑