BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,242,022	C→A	12.8%	C78F (TGC→TTC)	xylE ←	D‑xylose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,242,022	0	C	A	12.8%	241.9 / 2.5	108	C78F (TGC→TTC)	xylE	D‑xylose transporter
Reads supporting (aligned to +/- strand): ref base C (56/35); new base A (2/14); total (59/49)
Fisher's exact test for biased strand distribution p-value = 2.98e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

GGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGAC  >  NC_000913/4241788‑4242260

gaaaccggTTCCAAAATATGAATTGGGGAGAG‑ATTGGAGGTAAA‑CCAAACGCCAAAACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAAGAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAAt                                                                                                                                                                                                                                              <  1:406767/231‑1 (MQ=255)

      aGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTAAACCCGAAATAAAAAACAGGCCAGAAGCAATCTTAAGTGAATCACAACGACCGAA‑CAGGTTACAGAAAAAaccaa                                                                                                                                                                                                                                        >  2:1023414/1‑237 (MQ=255)

           cGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAGATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTACGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAACAATCTTAATGAAATCACGACAACCAAA‑ACGATAAATGCAAAAAAAACCAAggg                                                                                                                                                                                                                                  >  2:490370/1‑239 (MQ=255)

                 ataCATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCACGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAACAACCAACAAGGGCAcacc                                                                                                                                                                                                                            >  1:586862/1‑236 (MQ=255)

                     aTTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCcat                                                                                                                                                                                                                        >  1:1192898/1‑239 (MQ=255)

                     aTTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAAACTCAACCAGAAATAAAAAACAGGACAGCAGACATCTTAAGTGAATAACGACGAACGAA‑GCGGTTACTGCAAAAAACACCAAGGGCAcccccgga                                                                                                                                                                                                                        >  2:995924/1‑237 (MQ=255)

                       tGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCCCGACGACCGAA‑GCGGTTACTGCAAAAACCACCGAGGGCAACGCCgatga                                                                                                                                                                                                                      >  2:612085/1‑239 (MQ=255)

                       tGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                                                                                                                                                                                                                      >  1:997382/1‑239 (MQ=255)

                          cGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCCGAACCTACACCAGAAATAAAAAACAGGAAAGCAGCACTCTTAAGTGAATCACGCCGACCGAA‑ACGGTTAATGCAAAAACCACCCAAGGCACCCCCgataaggc                                                                                                                                                                                                                   >  2:914518/1‑234 (MQ=255)

                          cGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGGGAATCACAACGACCGAA‑GCGGTTACTGCAATAACCACGCAGGGCACCGCCgatgtagc                                                                                                                                                                                                                   >  1:637969/1‑235 (MQ=255)

                           gggagTATTGGGGGTAAACCC‑AACCCAAATACCGCAAATAAGGCGATAAATAACAAATTCCGGG‑A‑AATAACCGGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGatgatg                                                                                                                                                                                                                    <  1:112563/236‑1 (MQ=255)

                            agagCATTGCGGCTAAA‑CC‑AACGCCAATACCGACAATAATGCGATAAATAACAAATTACGGG‑A‑CATAACCCGCCAGATAAAACGGCACAGTG‑TT‑GTCCGAGTTT‑ATAGAGGC‑ACAA‑CCACGTTCTGGCCAGGCAGAACCTACACCAGAAATCAAAAAAAGGACAGCAGACATCTTAAGTGAACCACACCCAACGAA‑GCGGTATCATCAATACCCAACAAGGGGACCACAGAGGATGaaa                                                                                                                                                                                                                 >  2:1062008/1‑239 (MQ=255)

                            agagCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCCGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGCCGACCAAA‑AAGGTTACTGCAAAAACCACCGAGGGAACCGCCCATGAGGCaa                                                                                                                                                                                                                 >  2:146755/1‑239 (MQ=255)

                              agCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAATAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGCAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAcc                                                                                                                                                                                                               >  1:632225/1‑239 (MQ=255)

                              agCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAACCTTAAGGAAATCCCGACGACCGAA‑GCGGTTACTGCAATAACCACCAAGGGCACCGCCGATGATCAAAcc                                                                                                                                                                                                               >  2:1023108/1‑239 (MQ=255)

                                        aaaaa‑CC‑AACCCAAATCCCGCCAATAATGCGATAAATAAAAAATTCCGGG‑A‑CATAACCTGCCAGAAAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAAAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAgcg                                                                                                                                                                                                     <  1:1023108/237‑1 (MQ=255)

                                          aaa‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGCCGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCCCCGCCGATGTTGCAACCAATCaaggc                                                                                                                                                                                                      >  1:330974/1‑232 (MQ=255)

                                            a‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCCAAGCGCTg                                                                                                                                                                                                  >  1:27840/1‑238 (MQ=255)

                                                   cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACg                                                                                                                                                                                            >  1:482197/1‑239 (MQ=255)

                                                      aaagcccGCCAAAAATGCAAAAAAAAAAAAATTCCGGG‑A‑AATAACCTGCCAGAAAAACAGGCACATTG‑TT‑GTCGGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                                                                                                                         <  1:1023414/234‑1 (MQ=255)

                                                       caaccccccAAAAAGGGGAAAAAAAAAAAATGCCGGG‑ATCTAAACCAGCAAGA‑AAACAGGCACAGGGTTT‑GCCCGGGTTA‑AGAAGGGA‑AAAA‑AAAATTCCGGGACAGGAAAGACAAACACAAAAAAAAAAAACCAGGACGACAACAATTAGGAGGGAACACCGACGCACGAG‑GCGTTGAAGGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCaa                                                                                                                                                                                         <  2:1181495/232‑1 (MQ=255)

                                                          aCCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCAACGCAAAAc                                                                                                                                                                                      >  1:43893/1‑238 (MQ=255)

                                                                 ataatGCTATAAATAA‑AAACTCCGGGAA‑TATAACCCAGCAGATAAA‑AGGCCCAAGG‑TTGGTCCGTGTTA‑AAAGAGGA‑AAAA‑CCAATTTCGGGCCAGGAAGAACCTACACCAAAAATAAAAAACAGGAGACCAGAAATCTAAAGGGAACCACGACGACCGAA‑GTGGTTACTGCAAGACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAg                                                                                                                                                                              <  2:824524/239‑1 (MQ=255)

                                                                     tGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCAGCCAAAACCCTAACAGGGAg                                                                                                                                                                          >  1:258847/1‑239 (MQ=255)

                                                                     agcggataataaAAAAATCCGGGAA‑CAAATCCTGCCAAAAAAACAGGCA‑AGTG‑GT‑TTCCGGGTTTAATAGAGAA‑AAAC‑CAAAGTCCGGGCAAGGGAGAACCTACACCAGAAAAAAAAAACAGGCCAGCACCAATCTTAAGGGACCCACGAGGACGGAG‑GCGTTTACTGCAATAACCACGGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGa                                                                                                                                                                           <  2:888931/232‑1 (MQ=255)

                                                                       ggATAAATAAAAAATCCGGGG‑A‑AATAACCGGACAGAAAAAAAGGCACAGTG‑TT‑GGCGGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCGGGCAAGGAAGAACATACACCAGAAATAAAAAACAGGACAGCAGAAAGCTAAAGGGAAGCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAg                                                                                                                                                                          <  1:616519/236‑1 (MQ=255)

                                                                       agctcaataaAATATTCCGGT‑A‑GAGAACCTGCGATACAAACAGGCACAGGG‑TT‑G‑CCTGGGAT‑AAAGAAGTGAAAA‑CCAGTTTCACACAGGGGAAAACTTGCCCAGGAAATAAAAAACAGAAAAGAACAAATTTTAAG‑GAAACCCGGCGGACGAAGGCGTTTACGGAAAAACCCCCCGTGGACACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAg                                                                                                                                                                          <  2:317517/232‑1 (MQ=255)

                                                                                aCAAATTCCGGG‑A‑AAAAACCTGCCAAATAAACAGGCACAGTT‑TT‑GTCCGGTTTT‑ATAGAGTT‑AAAC‑CCAAGTTCGGGCCAGGCAGAACCTACCCCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGCCCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGa                                                                                                                                                               <  1:914518/239‑1 (MQ=255)

                                                                                 cAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGGGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGCTGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGcgcaggat                                                                                                                                                              >  1:1103920/1‑232 (MQ=255)

                                                                                  aaaTCCCGGC‑A‑CATACCCTGCCAGAAAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAC‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGAtt                                                                                                                                                             <  1:1127663/239‑1 (MQ=255)

                                                                                    aTTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGGGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCAAGGGCACCGCCGAGGAGGCAACCCCTCAGAGCCCTGGCCCCGAACAACACAAAAAGGGAGAGGGCAGGGGATACa                                                                                                                                                           >  2:965325/1‑239 (MQ=255)

                                                                                                    ccTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTgg                                                                                                                                             >  1:489521/1‑239 (MQ=255)

                                                                                                          aaaaaaccgggCACGGTG‑TT‑GTCCGGGTTT‑AAAGAGGA‑AAAC‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACGGCAGCAATCTTAAGGGAATCACGACGACCAAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGcaac                                                                                                                                       <  1:429288/230‑1 (MQ=255)

                                                                                                          aaaTAAACGGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGTT‑AAAC‑CCAAGTTCTGCCCAGGCAAAACCGACACCAGAAATAAAAACCAGGACAGCAGCAATCTTAAGGGAATCACGACGACCGAA‑GCGTTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAACCCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGcaac                                                                                                                                       <  1:1117377/237‑1 (MQ=255)

                                                                                                            aTAAACAGGCACAGTT‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAC‑CCAAGTTCGGGCCAGGCAAAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGcaacaa                                                                                                                                     <  1:612085/239‑1 (MQ=255)

                                                                                                            aTAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCCATAACCACCGAGGGAACAGCCGATGCTCCACCCAATCAGAGCGCTGGCCCAGAAAAACCCACACAGGGAGTGGGCAAAGGATTAACATAAGTTTTGAGGaaaaaaaa                                                                                                                                     >  2:129559/1‑232 (MQ=255)

                                                                                                            aTAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGCAGTTGGCAGCGACTTCACTTAAGTTTTGTGGAGcaacaa                                                                                                                                     >  1:1170031/1‑239 (MQ=255)

                                                                                                            aTAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGA‑AAAA‑CCAAGTTCTGGCCAGGCAGAACATACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGATTACGGCAATAACAACCGAGGGGACCACCGATGATGCAACCAATAGTACCACGGGCACGGCGAAACCCTAACAGGGAGCTGGCAGGGGATTAACATAAATTTTGTGGAGcaaaaa                                                                                                                                     >  2:81597/1‑239 (MQ=255)

                                                                                                               acacgggcaggtg‑tG‑GTCGGGGTTT‑AAAGAGTT‑AAAA‑CCAATTTCTGGCCAGGACAACCCTACACCAGAAATAAAAACCAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGa                                                                                                                                  <  1:806521/229‑1 (MQ=255)

                                                                                                                    gCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCATCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACggga                                                                                                                             >  1:997057/1‑237 (MQ=255)

                                                                                                                      acaGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATAAGGGCTCTGGCCACGCAAAACCCTAACAGCGAGTTGGCAGAGGATTCACTTCAGTTTTTGGGGGAACCAAAAACgggaat                                                                                                                           >  2:87325/1‑235 (MQ=255)

                                                                                                                         ttg‑tt‑gtCCGGGTTG‑AAAAGGGT‑AAAA‑CCAACTTCTGGCCAGGCAAAACCAACCCCAAAAAAAAAAAACAGGACAGTAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAg                                                                                                                       <  1:1131199/238‑1 (MQ=255)

                                                                                                                          tg‑tt‑gtCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGAATT‑GAgg                                                                                                                      >  1:90867/1‑238 (MQ=255)

                                                                                                                             tt‑gtCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAAACGGAATT‑GAGTg                                                                                                                     >  1:630485/1‑238 (MQ=255)

                                                                                                                                gtCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGGTTCACTTAAGTTTTGTGGAGCAACAAAGCCGGTATT‑GAGTGAc                                                                                                                   >  1:565166/1‑238 (MQ=255)

                                                                                                                                gtCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAAACGGAATT‑GAGTGACt                                                                                                                  >  1:524795/1‑239 (MQ=255)

                                                                                                                                gtCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAAACGGAATT‑GAGGGACt                                                                                                                  >  1:996128/1‑239 (MQ=255)

                                                                                                                                 tCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGGGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCAACACAAAACCCTAACAGGGAGTTGGGAGCGGATTCACTTAAGTTTTGTGGAACAACAAAGACGGTAAT‑GAGTGaacc                                                                                                                 >  2:716570/1‑236 (MQ=255)

                                                                                                                                     cggttttaaaagGT‑AAAACCCAAGTTCTGGCCGGGCGAAACCTACACCAAAAAAAAAAAACGGTACAGCAGCAAGCTTAAGTGAACCCCCACGACCGAA‑GCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCaa                                                                                                               <  1:134604/229‑1 (MQ=255)

                                                                                                                                      gTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTCACAGGGAGTTGGCAGCGGATTCCCTTAAGTTTTGTGGAGCAACAAAGACGGTGTT‑GAGTGACTCAACAg                                                                                                            >  1:1045963/1‑239 (MQ=255)

                                                                                                                                      gTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGGGCAACAAAGACGGAATT‑GGGTGACCCAACaa                                                                                                            >  1:1024795/1‑238 (MQ=255)

                                                                                                                                      gTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAg                                                                                                            >  1:619949/1‑239 (MQ=255)

                                                                                                                                      gTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCCAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCTAAACCCTAACCGGCGGTTGGCAGCGGATTCCCTTAAGTTTTGTGGAGCACCAAAGAGGGTATT‑GAGTGCCTCAAAAg                                                                                                            >  1:1227995/1‑239 (MQ=255)

                                                                                                                                            taGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCg                                                                                                       >  1:796930/1‑239 (MQ=255)

                                                                                                                                            taGAGGA‑AAAA‑CAAAGTTCGGGCAAGGCAGAACCTAACCCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGCCCGAA‑GCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCg                                                                                                       <  1:665844/239‑1 (MQ=255)

                                                                                                                                                 gT‑AAAA‑CCAAGTTCTGGCCCGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATCATGCAACCAATCCGAGCGCTGGCCACGCCAAACCCTAACAGGGAGGTGGCAGCAGCATCACTTAAACTTTGTGGAGAAACAACAACAGCATT‑GAGTGTATCAACAAAAACGGAAAt                                                                                                  >  2:450832/1‑239 (MQ=255)

                                                                                                                                                 gT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGACTCACGACGACCGAA‑GCGGTTACTGCAATAACAACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCCCGCAAAACCCTAACAGGGCGTTGGCAGCGGATTCCATTAAGTTTTGTGGAGCAACAAAGACGGAATT‑GAGTGACTCAAACGTACCGGAAAt                                                                                                  >  1:488848/1‑239 (MQ=255)

                                                                                                                                                 gT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACACTAACAGGGAGTTGGCAGCCGATTCACTTAAGTTTTGTGGCGCAACAAAAGAGGTATT‑GAGGGAATCAAAATAACAGGaaaa                                                                                                  >  2:441094/1‑238 (MQ=255)

                                                                                                                                                   gaaaa‑CCAAGTTCTGGCCAGGCAGAACATACACAAGTAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATTGCGACGACCGAA‑GCGGATACTGCAATAATCACCGAGGGCACCGCCGATGATGGAACAAATCAGAGGGCTGGCCACTCAAAACCCGCACCTGGGTCGGGCAGCGGAGTCACTTAAGGTTTGTGAAGCCACAAAAAAGGTATT‑TAGCGACTTCACATAAACGGaaaaa                                                                                                 >  2:269760/2‑237 (MQ=255)

                                                                                                                                                            aGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACCGGGAGTTGGCAGCGGCGTCACTTAAGTTTTTTGGAGCAAAAAAGACGGAATT‑GAGTGACTCAACCTTACCGGAAATAACGGCGgt                                                                                         >  1:714248/1‑239 (MQ=255)

                                                                                                                                                             gTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCCTTCAGTTTTATGGAGCAACAAAAACGGTATT‑GAGCGACTCAACAGTACCGGCAATAAaggcggag                                                                                        >  1:1249206/1‑237 (MQ=255)

                                                                                                                                                             gTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCCCTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGGGACTCAACAGTACCGGAAATAAcggcgggt                                                                                        >  1:946903/1‑237 (MQ=255)

                                                                                                                                                              gtcgggacAGGAAAAACATACACCAGAAAAAAAAAACAGGACAGCAGAAATCTTAAGGAAACCACGACGCCCGAA‑GCGGTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGgtgt                                                                                       <  1:1093498/232‑1 (MQ=255)

                                                                                                                                                                  ggCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAAACAATCAGAGCGCTGGCCACGCCAAACCCTAACAGGGAGTTAGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGTTATT‑GAGTGACTACACAAGAACGGAAAACAcggcgggatggag                                                                                   >  2:812509/1‑232 (MQ=255)

                                                                                                                                                                   gCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACTCAAAACCCTAACAGGGAGTTGGCAGCGTATTCACTTAAGTTTTGTGGCGCAAAAAAGACGGTATTAGAG‑GACGCAACACAACCGGAAATAAcgtcgggggtggac                                                                                  >  2:1019353/1‑231 (MQ=255)

                                                                                                                                                                   gCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGgttgccgt                                                                                   >  1:190449/1‑232 (MQ=255)

                                                                                                                                                                     cgggCAGACCCTAACCCAAAAAAAAAAAACAGGACCGCAGCAACTTTAAGTAAACCACGACGACCGAA‑GCGGTTACTGAAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGcc                                                                                <  1:490370/237‑1 (MQ=255)

                                                                                                                                                                     cggcgaacaCCTACCACAAAAAAAAAAAGCAAGACAGCAGAACTTATAAGTGAACCCGCACGACCGAA‑GGGTTTACGGAAACACCCACCGAGGCACCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGcc                                                                                <  2:489521/231‑1 (MQ=255)

                                                                                                                                                                        gCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGCGGGCACCGCCGATGATGCAACCAATCTGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGGGCAACAAAGACGGTATT‑GAGTGACTCAACCGTACCGGAAATAACGCCGgtgcgtgctgccaa                                                                             >  1:1137047/1‑228 (MQ=255)

                                                                                                                                                                        gCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACt                                                                                                                  >  1:954687/1‑202 (MQ=255)

                                                                                                                                                                             aCCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACa                                                                                                             >  1:700086/1‑202 (MQ=255)

                                                                                                                                                                               ttaacacaaaaaGAAACAACAGGACATCAGCAATCTTAAGTGAACCAGGCCGACCGAA‑GCGGTTACTGAAAAAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  1:110459/230‑1 (MQ=255)

                                                                                                                                                                               tcaccccAGAAAAAAAAAACAGGACAGCAGCAACCTTAAGTAAATCACGCTGACCGAA‑GCGGTTACTGCAATAACCACGGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  1:81597/234‑1 (MQ=255)

                                                                                                                                                                               cTACACCAAAAAAAAAAAACAGGACAGCAGCAATCTAAAGTGAACCCCGCAAACCGAA‑GCGGTTACTGCAATAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  1:683109/239‑1 (MQ=255)

                                                                                                                                                                               accccccAAAAAAAAAATACAGAACACCGTCAATCTGAAGCGACCCCCGACACCAGAA‑GCGTGTACTCCAATCCCCCCCGAGGCGACCGCGGATGGGGCACCCAATCAGAGCGCTGGCCACGCAAAACACTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  2:671802/234‑1 (MQ=255)

                                                                                                                                                                                tACACCAGAAAAAAAAAACAGGACAGCAGAAATCTTAAGTGAATCACGACGCCCGAA‑GCGGTTCCTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  1:904119/238‑1 (MQ=255)

                                                                                                                                                                                  cacCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGGGGCGTAGCCAAAAAATaaaaaa                                                                   >  1:480191/1‑236 (MQ=255)

                                                                                                                                                                                          aaaaaaaaCAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGt                                                           <  1:146755/237‑1 (MQ=255)

                                                                                                                                                                                          aTAAAAAACAGGACAGCAACAATCTTAAGTGAACCACGACGCCCGAG‑GCGGTTACTGCAATAACCGCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGt                                                           <  1:1062008/239‑1 (MQ=255)

                                                                                                                                                                                           aaaaaacCAAGACACCAGCAAACATAATGTAACCACCGCGACCGAA‑GGGGTTCCTTCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTa                                                          <  1:514725/238‑1 (MQ=255)

                                                                                                                                                                                           aaaaaaaCAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACCGAAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATaaa                                                                      <  1:10672/226‑1 (MQ=255)

                                                                                                                                                                                                         acacaaaTCTTAAGTAACACACCACCAACGAA‑GCTGTAACCCAAACAACCCCCGAGTACACCGCCGATGATGCAAACAATCAGAGCGCTGGCCTCGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACTGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAAt                                            <  2:1247864/234‑1 (MQ=255)

                                                                                                                                                                                                          cagcaATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGGGTCGTAGCCCAAAAATAAACCACCTACTTTTGGGACCAAGGCaaac                                           >  1:873067/1‑237 (MQ=255)

                                                                                                                                                                                                           agcaATCGTAGGTGAATCACGACGACAGAG‑GCGGTTACTGAAAGAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAAt                                            <  1:700261/237‑1 (MQ=255)

                                                                                                                                                                                                           agaaaTCTAAAGGGAACCCCGACGACCAAA‑GGGGTTACTGAAAAACCCCCCGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTa                                              <  1:1019353/232‑1 (MQ=255)

                                                                                                                                                                                                            aaaaTCTTAAGGGAACCCCGACGACCGAA‑GCGTTTACTGCAATACCCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGa                                         <  1:988493/237‑1 (MQ=255)

                                                                                                                                                                                                              aaTCTTAAGTGAATCCGGTCGGCCGAA‑GGGGTTACCGCAAAAACCCCCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGa                                         <  1:129559/237‑1 (MQ=255)

                                                                                                                                                                                                                       gaaaccccgccgacCGAA‑GCGGTTACTGCAATCACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGAAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                              <  1:1268784/229‑1 (MQ=255)

                                                                                                                                                                                                                        gaacaacgaccCCCGAA‑CGGGTTACTGCAAGAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                              <  1:1165428/233‑1 (MQ=255)

                                                                                                                                                                                                                        gAATCACGGCGACCGAA‑GCGGTAACGGCAATACCCCCGGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                              <  1:492906/238‑1 (MQ=255)

                                                                                                                                                                                                                        aaaacccgacggCCGAA‑GCGTTTACGGAAAAAACCACCGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                              <  1:87325/232‑1 (MQ=255)

                                                                                                                                                                                                                        aaaacagcacgacCGAA‑GCGGTTCCTGCAATACCCACCGAGGTCACCGCCGATGAGGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                              <  1:450832/230‑1 (MQ=255)

                                                                                                                                                                                                                         aaTCACGACGACCGAA‑GCGGTTCCGCAAAAAACCCCCGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAAc                              <  1:990320/237‑1 (MQ=255)

                                                                                                                                                                                                                          aTCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTACGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGAGTCGGAGCCAAAAAAAAAAACACCCAAAGAAAAGACCAAGGGAAAGGaaaaaaaaaaaaaa                            >  2:988493/1‑228 (MQ=255)

                                                                                                                                                                                                                            cctgacgccCGAA‑GCGGTTCCTGCAATACCCACGGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGaa                         <  1:681883/236‑1 (MQ=255)

                                                                                                                                                                                                                                 cgacCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAg                                                                                                                                   <  2:684123/129‑1 (MQ=255)

                                                                                                                                                                                                                                 cgacCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAg                                                                                                                                   >  1:684123/1‑129 (MQ=255)

                                                                                                                                                                                                                                         aggtgTTACCGCAATAACCACCGGGGTCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTa             <  1:294803/235‑1 (MQ=255)

                                                                                                                                                                                                                                            ggTAACTGAAATACCCACCGAGGGACCCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTat            <  1:1158309/237‑1 (MQ=255)

                                                                                                                                                                                                                                                    cAATACCCCCCGGGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGAc  <  1:965325/239‑1 (MQ=255)

GGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAA‑CC‑AACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGG‑A‑CATAACCTGCCAGATAAACAGGCACAGTG‑TT‑GTCCGGGTTT‑ATAGAGGT‑AAAA‑CCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAA‑GCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTGGCCACGCAAAACCCTAACAGGGAGTTGGCAGCGGATTCACTTAAGTTTTGTGGAGCAACAAAGACGGTATT‑GAGTGACTCAACAGTACCGGAAATAACGGCGGTGTCGTAGCCAAATAATAAACCACCTAATGTAGCGACTAAGGTAATCGAAAATATATAACTGGAATTATACTGGGTATTCATTCAGAC  >  NC_000913/4241788‑4242260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`