Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,936,648	C→T	62.2%	intergenic (‑334/‑4)	zwf ← / → yebK	glucose‑6‑phosphate 1‑dehydrogenase/putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,936,648	0	C	T	62.2%	37.7 / 45.8	45	intergenic (‑334/‑4)	zwf/yebK	glucose‑6‑phosphate 1‑dehydrogenase/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (9/8);  new base T (15/13);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAAT  >  NC_000913/1936381‑1936928                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                         cACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGaaaaa                                                                                                                                                                                                                                                                     >  2:98665/1‑289 (MQ=255)         tGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAg                                                                                                                                                                                                                                                               >  1:226947/1‑287 (MQ=255)           cTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGtctc                                                                                                                                                                                                                                                           >  1:144584/1‑289 (MQ=255)                aCGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGCCAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTg                                                                                                                                                                                                                                                      >  2:72160/1‑289 (MQ=255)                aCGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCGGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTg                                                                                                                                                                                                                                                      >  2:211156/1‑289 (MQ=255)                                          tATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACTCTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCaa                                                                                                                                                                                                                            >  2:48556/1‑289 (MQ=255)                                            tCCACCCGTGCGATTACGGGAAAATCGCGCAAAGTGCGGCAAAACTGAAAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAg                                                                                                                                                                                                                          <  2:169461/289‑1 (MQ=255)                                                 ccGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAATTCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGcc                                                                                                                                                                                                                     >  2:406011/1‑289 (MQ=255)                                                 ccGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGcc                                                                                                                                                                                                                     >  1:41333/1‑289 (MQ=255)                                                   gTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGa                                                                                                                                                                                                                   >  1:373166/1‑289 (MQ=255)                                                                     cgcgcAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGACCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCAAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGc                                                                                                                                                                                                  >  2:537894/1‑288 (MQ=255)                                                                               gCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAAcg                                                                                                                                                                                         <  2:373166/287‑1 (MQ=255)                                                                                        cTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCg                                                                                                                                                                              <  1:98665/289‑1 (MQ=255)                                                                                                                      tAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCaa                                                                                                                                                 <  1:405878/288‑1 (MQ=255)                                                                                                                      tAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCaa                                                                                                                                                 <  2:361356/288‑1 (MQ=255)                                                                                                                                      aTCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTCTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGTGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACgg                                                                                                                                >  2:120171/1‑289 (MQ=255)                                                                                                                                              aTGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGt                                                                                                                        >  2:327151/1‑289 (MQ=255)                                                                                                                                              aTGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGt                                                                                                                        >  2:298642/1‑289 (MQ=255)                                                                                                                                                gAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTTCTATGGCACTGGAAGCCAATGTTACCAAACCGACGGTGAATCGttt                                                                                                                      >  1:60291/1‑289 (MQ=255)                                                                                                                                                                     tATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCGGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACAcgcg                                                                                                 <  1:211156/289‑1 (MQ=255)                                                                                                                                                                     tATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACAcgcg                                                                                                 <  1:123583/289‑1 (MQ=255)                                                                                                                                                                          gCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGtt                                                                                            >  1:331377/1‑289 (MQ=255)                                                                                                                                                                                        cacGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTaaa                                                                              <  2:144584/289‑1 (MQ=255)                                                                                                                                                                                           ggCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACt                                                                            >  2:99716/1‑288 (MQ=255)                                                                                                                                                                                             cacacaGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCa                                                                         >  1:64491/1‑289 (MQ=255)                                                                                                                                                                                              acacaGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCAt                                                                        <  1:298642/289‑1 (MQ=255)                                                                                                                                                                                              acacaGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCAt                                                                        <  2:331377/289‑1 (MQ=255)                                                                                                                                                                                                   ggCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTgg                                                                    >  2:474056/1‑288 (MQ=255)                                                                                                                                                                                                      gTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGcaca                                                                <  2:64491/289‑1 (MQ=255)                                                                                                                                                                                                      gTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAAGCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGcac                                                                 <  2:316634/288‑1 (MQ=255)                                                                                                                                                                                                              tGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCAt                                                                        <  2:259136/273‑1 (MQ=255)                                                                                                                                                                                                              tGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCAt                                                                        >  1:259136/1‑273 (MQ=255)                                                                                                                                                                                                              tGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTg                                                         >  1:554845/1‑288 (MQ=255)                                                                                                                                                                                                               gACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGc                                                       <  1:384973/289‑1 (MQ=255)                                                                                                                                                                                                                              cGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTa                                        >  1:296800/1‑289 (MQ=255)                                                                                                                                                                                                                                       tcAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGGCGCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGAGATCCATTCAAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCAGCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCg                               <  2:226947/289‑1 (MQ=255)                                                                                                                                                                                                                                           tGCGGAAATCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAAt                           >  2:78036/1‑289 (MQ=255)                                                                                                                                                                                                                                           tGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAAACGTTTCTGTCGCAGCATGGACCCGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAAt                           >  2:503604/1‑289 (MQ=255)                                                                                                                                                                                                                                            gCGGAAATCGTCATTACCCGTGAGTCTCTTTATTGCATGAAAATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGGGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCaa                            <  1:518828/287‑1 (MQ=255)                                                                                                                                                                                                                                                aaaTCGTCATTACCCGTGAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCa                       <  1:388585/288‑1 (MQ=255)                                                                                                                                                                                                                                                       cATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGAt               >  2:240491/1‑289 (MQ=255)                                                                                                                                                                                                                                                              cGTGAATCTATTTACATCATGAATATGCTGGAAAAAATCCAGCGTCAGCTGGAACATTAGAGCAAATCAGAGCGCTAAGTTGCCGAGGTCATTCTGGCGTCTCCCGATAACGCGATCCATTCGGGTATTGCTGCTATGGCACTTGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCg        <  2:463155/289‑1 (MQ=255)                                                                                                                                                                                                                                                                 gAGTCTCTTTATATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGtt      <  1:78036/288‑1 (MQ=255)                                                                                                                                                                                                                                                                 gAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGtt      <  1:537894/288‑1 (MQ=255)                                                                                                                                                                                                                                                                     ctctTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAAt  <  1:99716/288‑1 (MQ=255)                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                         CACTGTACTGCTTTTACGAGCTTGCGAAAACTGTAAACGCTTATCCACCCGTGCGATTACGGGAAAAGCGCGCAAAGTGCGGCAAAACTGATAAAAAAATCATCGTTTTCCATTGGGTAAAAATCTGACACTGATCATGTTATGAAAAAAAATAACAACTTTTTTATCTGCTTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTACCCGTGAGTCTCTTTACATCATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGCTTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTTTCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTATGTTAATCGCAATGTCAATGAAGATGACAGCGTTGAAT  >  NC_000913/1936381‑1936928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑