| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,011,944 | A→T | 5.8% | D290V (GAT→GTT) D291E (GAT→GAA) |
yigM → metR ← |
putative inner membrane EamA‑like transporter methionine biosynthesis regulon transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,011,944 | 0 | A | T | 5.8% | 235.9 / 3.4 | 69 | D290V (GAT→GTT) D291E (GAT→GAA) | yigM metR | putative inner membrane EamA‑like transporter methionine biosynthesis regulon transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (32/33); new base T (2/2); total (34/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
GCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGA > NC_000913/4011681‑4012226 | gCAATGGGGCGTGCTGGTGTTGCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAg < 2:189332/288‑1 (MQ=255) cATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACGTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAAc < 1:262084/288‑1 (MQ=255) ttCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCCCGCCGGTGCTCCTGGCCTCACCGTGGGTGCCTCGTAAGTGGGTTCCCACCG‑GCCCTTCACAACCGGCACATGATCTCAGGCTTGATTCGGCGCTGTGCGAATACACGc > 2:262084/1‑288 (MQ=255) gcTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCtt < 2:475681/288‑1 (MQ=255) gtTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTaa < 2:268541/289‑1 (MQ=255) cTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTCCAGGCGCGCTGGTGATCCTGGCCTCACTGTTGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGGGATTGCGCGCTGCGCGAATAAACGCTTCAGTACCTg > 2:422159/1‑288 (MQ=255) cTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATaacc > 2:105394/1‑273 (MQ=255) cTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAAc < 1:105394/275‑1 (MQ=255) gCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTg > 1:139454/1‑288 (MQ=255) gtggCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACgct > 1:271692/1‑288 (MQ=255) tatttgttggggAACTACGGCGCGACGCAGGTGGACGCGGGAACGCGGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGTCCAACGTTTATTACAGGCGCGCTGGTGATCCGGGCCTCACTGTGGGGGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑cgg < 2:484799/278‑1 (MQ=255) tttATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACTCTGCCCGCCCTCGCGCA‑Cggcggc > 2:432872/1‑289 (MQ=255) ttATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGTTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGgcggcg < 2:114683/289‑1 (MQ=255) tATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGt > 1:533069/1‑289 (MQ=255) aCTCCGGCGGGACGCAGGTGGAGGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCgg < 2:525184/289‑1 (MQ=255) cGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGAGGAGCGCAGGCCGGATTGCGCGCTGTGCGAATCAACGCTTCCGTAACTGGCTGACGCTTCTACCCTGCGCGCC‑CGGCGGCGTaaacgcggccc > 2:231053/1‑278 (MQ=255) cGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCAACAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGTTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGCTGATCGCAGGCGTGATTGCGCGCTGATGGAACAAACGCATCCGTAAACGGCTGACGCTGCTTGCCAT‑GCACACCGGCGGCTGACAGTGGGccc > 2:88745/1‑286 (MQ=255) cgcgACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCcac < 2:65260/288‑1 (MQ=255) cgACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTTGCTGCCGCTGCTCGCCATCGCGCA‑CGGCGGCGTACCGTCGGCCCCACaa > 2:234176/1‑288 (MQ=255) cGCAGGTGGGAGCCGGAAGGCTGGGCGTTATGAATAATAGGAACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTAGTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGcc < 2:271692/288‑1 (MQ=255) tGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGcc < 1:215049/288‑1 (MQ=255) cGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAggg > 1:86672/1‑289 (MQ=255) cGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCtt > 2:547298/1‑277 (MQ=255) cGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCtt < 1:547298/277‑1 (MQ=255) cGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGttt < 2:326980/289‑1 (MQ=255) cGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGtt < 2:49012/288‑1 (MQ=255) aCGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGt < 1:422159/289‑1 (MQ=255) aCGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGt < 1:432629/289‑1 (MQ=255) gCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTca < 2:86672/289‑1 (MQ=255) tGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTcac < 1:231053/288‑1 (MQ=255) gAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGAc > 1:528032/1‑286 (MQ=255) ataataTGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTg > 1:95654/1‑288 (MQ=255) taagaTGCGCGTTCCGGCAGGGCTGCTGGTAAACCTGGCTAGCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGgc < 2:426142/289‑1 (MQ=255) tGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCa < 1:88745/289‑1 (MQ=255) cGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCG‑CCAGGGTTTTGGTCACCACCAGACCCTGGCGCCCAAAAc > 1:291225/1‑288 (MQ=255) gTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAAct < 1:328647/289‑1 (MQ=255) ttCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAAct < 2:533069/288‑1 (MQ=255) tCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTc > 2:304967/1‑261 (MQ=255) tCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTc < 1:304967/261‑1 (MQ=255) ggCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTTACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCtcctac > 1:406122/1‑288 (MQ=255) ggCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCcaca < 1:318548/230‑1 (MQ=255) ggCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCcaca > 2:318548/1‑230 (MQ=255) ctgGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGTTCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAAt > 2:190212/1‑288 (MQ=255) aaaCCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTa > 1:85379/1‑289 (MQ=255) ccTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAgcg < 1:537260/289‑1 (MQ=255) tATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCgg < 2:258420/204‑1 (MQ=255) tATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCgg > 1:258420/1‑204 (MQ=255) cgcacacgagCCAAGGTTTATTCCCGGCGCGTGGGTGTTGCTGCACTCACTTTGGGTGCAGCTTAGGTGGT‑TCACTCCGCGCTCTTCACAAGCGGCAAATGATCGCAGGTGTGTTTGCGCGCTGAGCGAATAAACGCTTCCGGAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCcgcgcg < 2:231294/279‑1 (MQ=255) ttattaCAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGTTCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATcaa < 1:190212/288‑1 (MQ=255) attaCAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCa < 2:178947/153‑1 (MQ=255) attaCAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCa > 1:178947/1‑153 (MQ=255) attaCAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATcaacaa > 1:179000/1‑289 (MQ=255) ttaCAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAAt < 1:145113/289‑1 (MQ=255) gcgcgcTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCCTCTGAATCAACAATAAGgtt > 1:521271/1‑288 (MQ=255) ccTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCcgcg > 1:63145/1‑242 (MQ=255) ccTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCcgcg < 2:63145/242‑1 (MQ=255) ccTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAAt > 2:512873/1‑265 (MQ=255) ccTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAAt < 1:512873/265‑1 (MQ=255) ccTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGt > 1:74011/1‑289 (MQ=255) tCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGa < 1:432872/289‑1 (MQ=255) aCTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCCCAAAACTCTCCACTACCCAATGCGGTCGCACGGCAATACCCATCCGCCCGGCAACCATCTGAATCCACAATAAGGTGTTTACCACCCTTTTCAGTGACg > 2:172253/1‑289 (MQ=255) cATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGc > 1:175795/1‑288 (MQ=255) gg‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTgaa > 2:73706/1‑289 (MQ=255) tCGCTCCGCGCTCTTCACAAACGGCAGATGTTCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGAcc > 2:41435/1‑159 (MQ=255) tCGCTCCGCGCTCTTCACAAACGGCAGATGTTCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGAcc < 1:41435/159‑1 (MQ=255) acgtgCAGATGATCGCGGCCGCGGTTGTGCGCTGAGCGAATAAACGCTTCCGCATATCGCTGACGCTGTTCGCCACAGCGCA‑CGGGGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCa < 2:528032/285‑1 (MQ=255) aCGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAg > 1:445429/1‑289 (MQ=255) cGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAg > 2:365586/1‑288 (MQ=255) gCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATCCCCATCCGCGCGGCAACCATCTGAATCAACAATACGGTGTTATCGACGCTTTTACGTGACGGGCCGACGCCTGCCGGCCGAAGCAAATGACGCCCGCCATCCacttc > 2:246558/1‑285 (MQ=255) ggatgagCGCAGGCGGGATTGCGCGCTGGGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGa < 2:139454/287‑1 (MQ=255) | GCAATGGGGCATTCTGGTGTTTCTTGGCGTGGTGGCTTCCGGGATTGGCTACTTTATGTGGAACTACGGCGCGACGCAGGTGGACGCCGGAACGCTGGGCATTATGAATAATATGCACGTTCCGGCAGGGCTGCTGGTAAACCTGGCTATCTGGCACCAACAGCCGCACTGGCCAACGTTTATTACAGGCGCGCTGGTGATCCTGGCCTCACTGTGGGTGCATCGTAAGTGGG‑TCGCTCCGCGCTCTTCACAAACGGCAGATGATCGCAGGCGTGATTGCGCGCTGAGCGAATAAACGCTTCCGTAACTGGCTGACGCTGCTCGCCATCGCGCA‑CGGCGGCGTACAGTCGGCTCCACAAGCCTTCGCCCAGGGTTTTGGTCACCACCAGACCCTGGCGCTCAAAACTCTCTACTACCCAATGCGGTAGCGCGGCAATACCCATCCGCGCGGCAACCATCTGAATCAACAATAAGGTGTTATCGACGCTTTTCAGTGACGGGCTGACGCCTGCCGGCTGAAGAAAATGCCGCCAGACATCCAGTCGA > NC_000913/4011681‑4012226 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |