New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2840867 =	35 (0.910)	3 (0.080)	3/268	5.2	8.2%	coding (1344/1458 nt)	ascF	cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
	?	NC_000913	2840899 =	34 (0.940)					coding (1376/1458 nt)	ascF	cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGA  >  NC_000913/2840732‑2841006                                                                                                                                        |                                                                                                                                            cGGTATGGCGGTGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTca                                                                                                                                      <  1:546943/143‑1 (MQ=255)            ggCTTGCCAGCCACTCGATGGCGGCGGCGGGGCTCTTGTCCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTc                                                                                                                           <  2:707757/143‑1 (MQ=255)             gCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCg                                                                                                                          >  1:690512/1‑143 (MQ=255)               ttGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATGCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGACGGACCCACTGTTGCTGGCGTTTATCCTCACACTGATGCTCAGc                                                                                                                        >  2:708492/1‑143 (MQ=255)               ttGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCACCGTGCACTTCTTCGATCCGACGAATCCAATAAGCATCGTCTAGGTGTTAGCGGTCATGGCGCTGGCGGTGGTACTGTCGTTTATCCTCACACTGTTGCTCGGc                                                                                                                        >  1:337922/1‑143 (MQ=255)                 gccagccaCTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGCATCCAATGAGCATAGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGGTTATCCTCCAACGGCTGCTCGGcgc                                                                                                                      >  2:365167/1‑141 (MQ=255)                            aaTGGCGGCCGCGGGGCTATTTCCCAGCGTTCAGTGCTTTGTTCCGGCGAATCCAATGAGCGTCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTc                                                                                                           <  2:68096/143‑1 (MQ=255)                                 cAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTCAGGATATTCCTGtt                                                                                                      >  2:20577/1‑143 (MQ=255)                                   gcgcCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGAGGTGCTGTCGTTTATCATCACACTGTTGCTCGGCTTTGCGGATCTTCCTGGTGa                                                                                                    >  2:388352/1‑143 (MQ=255)                                          ggCTATTTAGTAGTGTGCATTTCTTTTTTCCGTTTAATTCTTTGAGTATCGTCTGGGTGTTCGCGGGCAGGGCGCGGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCg                                                                                             <  1:402094/143‑1 (MQ=255)                                           gctgcttACCGGCGCGCAGTTCGTCGCTCCGGCGACTCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCgg                                                                                            <  1:390880/138‑1 (MQ=255)                                             tATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCCGCTTTGAGGATATTCCTGTTGAGGACGCGGCt                                                                                          >  1:270218/1‑143 (MQ=255)                                               tttACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCCCCCTGTTGCTCCGCTTTGCGGATATTCATGTTGAATAAGCGGATGc                                                                                        >  1:438491/1‑143 (MQ=255)                                                ttACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCGGGCGGTGGTGCTAGCGTTTATCCTGACACTGTTGCTCGCCTAGGAGGCTAGTCCGGCTGAGGAGACAGCTGca                                                                                       >  2:415308/1‑142 (MQ=255)                                                          gCAGTTCTTCGATCCGGCGCATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGaa                                                                             <  2:523444/143‑1 (MQ=255)                                                           cAGTTTTTTGTTCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGAATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGaaa                                                                            <  1:644901/143‑1 (MQ=255)                                                                     aTCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGc                                                                  <  1:400105/143‑1 (MQ=255)                                                                              aTCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGACCAGACGCAAAAGTATCACAGCGTAAAacgg                                                         >  1:354343/1‑141 (MQ=255)                                                                               gccAATGGGCATCGTCGGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAaccga                                                        <  1:538415/142‑1 (MQ=255)                                                                                ccAATGTGCTTCGTTTGGGTGTTCGCGGTTATGGCGCTGGCGGTGGTGCTGTCGTGTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAaccgac                                                       <  1:717506/143‑1 (MQ=255)                                                                                  aaTGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGAGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGGAGCGGCTGCCCACGCGCGAAATTATCAGAGCGTACAaccgaccg                                                     >  2:613270/1‑143 (MQ=255)                                                                                        cATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGt                                                                <  1:662026/126‑1 (MQ=255)                                                                                        cATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGt                                                                >  2:662026/1‑126 (MQ=255)                                                                                           cGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACGGTTGCTCGGCTTTGCGGATATTCCTGTTGAGGAAGCAGCAGCACAGACGAGAAAGTATCAGAGCGTACAACCGACCGTCGCCGaag                                            >  1:545356/1‑143 (MQ=255)                                                                                                gggTGTTCGCGGTCATGGCGCTGGCGGAGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAAGAAGCGGCTGACCACACGCGAAAGTGTAAGAACTTACAACCGAACGTCGCCAAAGaagta                                       >  2:344795/1‑143 (MQ=255)                                                                                                            ttctGGGGCTGGCGGTGGTGCTGTCGTTTATCCTCTCACTTTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCTAAAGAAGTAAGTCTTAATTGa                           <  2:566924/140‑1 (MQ=255)                                                                                                               tGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTGTCAGCGCGTACACCCGCCCGTCGCCAAAGAAGTAAGTCATACATGAGGa                        >  1:82364/1‑143 (MQ=255)                                                                                                                  cgcTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTGTCAGAGCGTGCTGCCGGCCGTCGCCGTAGGTGTGTGTCCTATTTGAGGATGa                     >  1:33/1‑143 (MQ=255)                                                                                                                      ggcgagtgtgCTGTTGTTTATCTTAACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAGCCGACCGTCGCCAAAGAAGTAAGTCATAATTGAGGATGAAAAt                 <  2:29095/136‑1 (MQ=255)                                                                                                                          ggggggCTGTCCTTTATCCTCCCCCTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAGGCGGCTGCCCAGGCGCGAAAGTATTAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAACTGTCa             <  2:356152/138‑1 (MQ=255)                                                                                                                           tgttgttGTCGTTTATCCTTTCACTGTTTCTTGGTTTTGAGGATATTGCGGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAg            <  1:20577/137‑1 (MQ=255)                                                                                                                                 tggcGTTTATCCGCGCGCTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGAGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTc      <  2:476316/140‑1 (MQ=255)                                                                                                                                  gTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGGAAGTCTAAATGGAGGATGAAAATGTCAGCATATcc     >  2:67367/1‑143 (MQ=255)                                                                                                                                     gTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAGGGAGATCAACTTAAATGAGGATGAAAATGTCAGTATTTCCAGa  >  2:315704/1‑143 (MQ=255)                                                                                                                                     gTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGGAGCAGGTCCGCACTGAGGAGCAAAAGGCCATGATTTCCAGa  >  2:168983/1‑143 (MQ=255)                                                                                                                                        |                                                                                                                                            CGGTATGGCGGGGCTTGCCAGCCACTCAATGGCAGCGCCGGGGCTATTTACCAGCGTGCAGTTCTTCGATCCGGCGAATCCAATGAGCATCGTCTGGGTGTTCGCGGTCATGGCGCTGGCGGTGGTGCTGTCGTTTATCCTCACACTGTTGCTCGGCTTTGAGGATATTCCTGTTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGA  >  NC_000913/2840732‑2841006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.