New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 330771246 (1.200)3 (0.080) 3/272 5.3 6.4% coding (149/1890 nt) deaD ATP‑dependent RNA helicase
?NC_000913 = 3307717 43 (1.170)coding (144/1890 nt) deaD ATP‑dependent RNA helicase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAAT  >  NC_000913/3307570‑3307852
                                                                                                                                              |                                                                                                                                            
gacgtCCGTCATTTCCTCAGCAACCTGTACTGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCGCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTggg                                                                                                                                              <  2:227998/139‑1 (MQ=255)
gaAATCCGTCATTGCTTCAGCATCCTGTTCCGCCTGTTCGCGGTTCTTTTCTAGCATTAGAATCTGTGGTGCTGTCAGCGCAGGATCAAGAGGCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTggg                                                                                                                                              <  1:173750/143‑1 (MQ=255)
 gagtgcGTGATTGCTGCAGCAAGCTGTGCCGCCGGTGGGCGGGTCGGTGCCAGCGCCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGc                                                                                                                                             <  2:414887/138‑1 (MQ=255)
 aaaTCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCGACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCGCCGTCTgcgc                                                                                                                                             >  2:462649/1‑143 (MQ=255)
 aaaTCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCACCAGAGGTAAAGAGAATGCTGCAGTTTTTCCTCTACCCGTCTGGGc                                                                                                                                             >  1:440454/1‑143 (MQ=255)
    tCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTAAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGGCag                                                                                                                                          >  2:462702/1‑142 (MQ=255)
              cTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGCTGCCATCCGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGCACg                                                                                                                                >  2:477729/1‑143 (MQ=255)
               ttCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTACAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGt                                                                                                                               >  2:297920/1‑143 (MQ=255)
               ttCAGCAACCTGTACCGCCAGTTCGCGAGTCGGTGCCAGCACCATAATCTGTGGTGATTTCAGCTCAGGATCAAGATTCTGCAACAAAGGTAAAGAGAATGCTGCAGATTTTCCGCTCCCCGCCGGAGCCATACCCAGAACga                                                                                                                               >  2:541130/1‑142 (MQ=255)
                    cAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCgg                                                                                                                          <  1:571609/143‑1 (MQ=255)
                       ccagTACCGCAAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCa                                                                                                                       <  1:309631/140‑1 (MQ=255)
                                      cgcgGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGAGCAAGATTCTGCAACAGAGGTAAAGAGAATGCGGCGGTTTTTCCGCTGCACGTGGGGGCCATGCCCACGACGTCGCGGCCATTCAGCAGATGTGGa                                                                                                        >  2:595765/1‑143 (MQ=255)
                                           gccGGTGCGAGCACCAGGTTCGGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAGTGCTGCAGGTTTGCGGCTCCCCGTCTGGGCCATAGCCAGAACTTCGCGGCCATGCAGCAGATGTGGAATaca                                                                                                   <  2:259049/141‑1 (MQ=255)
                                           gTCGGTGCCAGCATCAGAAGCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATaca                                                                                                   <  2:39720/143‑1 (MQ=255)
                                           gTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCCGCAGATGTGGAATaca                                                                                                   >  2:229635/1‑143 (MQ=255)
                                                  ccAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCCTTCAGCAGATGTGGTGTCCACTCTGcc                                                                                            >  1:587967/1‑143 (MQ=255)
                                                   cAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCCTTCAGCAGATGTGGAATACACTCTGCCt                                                                                           >  1:712621/1‑143 (MQ=255)
                                                      cACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGaa                                                                                        >  1:417789/1‑143 (MQ=255)
                                                       aCCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTACAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATCCACTCTGCCGGagt                                                                                       >  1:92616/1‑141 (MQ=255)
                                                        ccAGAATCGTAGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTGTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAAtt                                                                                      <  2:159946/143‑1 (MQ=255)
                                                         cAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTg                                                                                     >  2:458814/1‑143 (MQ=255)
                                                         cAGAATCTGACCTGCATTAAGCACAAGACAAAGAATCAACAACAGAAGAAAAGAGAAGGCTGCAGTATTTCCGCTCCCCGTCCGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTg                                                                                     <  1:241918/143‑1 (MQ=255)
                                                          aGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTgg                                                                                    >  1:270012/1‑143 (MQ=255)
                                                              tCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGAt                                                                                >  1:38960/1‑143 (MQ=255)
                                                                tgtgGTGCGTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATgg                                                                              <  2:107512/143‑1 (MQ=255)
                                                                 gtggtgCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATAAACTCTGCATGAATGAGAGATgat                                                                             >  1:711439/1‑141 (MQ=255)
                                                                            aGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAAcc                                                                  <  1:708475/143‑1 (MQ=255)
                                                                                   gATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACACAGATCg                                                           >  1:207018/1‑143 (MQ=255)
                                                                                    aTCAAGATTGTGCAACAGAGGTAAAGAGAATGCTGAAGTTTTTCCGCTCCACGTATGGGCCATACTCAGAACGTCGCGGCAATTCAGCAGATGTAGAATACAAAATGCAGGAATTTGAGCTGGATTTTCGTAACGAAGATAGt                                                          >  2:9207/1‑143 (MQ=255)
                                                                                      cAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCAAGAATGTCGCGGCCATTAAGCAGATGCGGAATACACTCTGCCTGAATTGGAGATGGTTTTTAGTAACCCAGATAGTTa                                                        >  2:477081/1‑143 (MQ=255)
                                                                                          aTTCTGCAAGAGAGGTAAGGAGAATGCCGGGTTAGACCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAggg                                                    <  1:688995/143‑1 (MQ=255)
                                                                                           ttCTGAAACAGAGGTAAAGAGAATGCTGGAGTTTTTCCGCTCCCCGGCTGGGCCATACCCCGAACGTCGCGGCCATTCAGCAGATGTGGAATACACCCTGCCTGAACTAGAGATGGTTTCTCGTAACCCAGATCGTCAAGGGc                                                   >  2:15360/1‑143 (MQ=255)
                                                                                                 aaCAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTACCAGACTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCAGCCTGAATTGGAGAGGATTGTTCGTAACCCAGAGCGTTAAGGGCTTCaac                                             >  2:480330/1‑142 (MQ=255)
                                                                                                     gagGTAAAGAGAATGCTGCAGTTTTTCCTCTCCCCGTCTGGGCCATTCTCAGATTGTCGTGTCTATTCAGCAGATGTGGAATACACTCGGCCTGAATTGGAGATGGTGTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATa                                         <  1:477140/143‑1 (MQ=255)
                                                                                                     gagGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATa                                         <  1:599474/143‑1 (MQ=255)
                                                                                                       gggaaaTAGGATGCTGCAGTGTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAAAAAAAAAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAgg                                       <  2:675682/140‑1 (MQ=255)
                                                                                                              agaATGCTGCAGTTTTTCCGCTCACCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCAAGATCGTTAAGGCCTTCTAGGATAGGAGCCtga                                >  2:469283/1‑141 (MQ=255)
                                                                                                                 aTGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCGGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAgg                             <  1:397652/143‑1 (MQ=255)
                                                                                                                   gctgcAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCCGCAGATGTGGAATACTCTCTGCCTGAATTGGAGATGGTTTTTCGTAACCAAGATAGTTAAGAGATTCAAGGATAGGAGCCTTCAGGcc                           >  1:9660/1‑143 (MQ=255)
                                                                                                                    ctgcAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTGTGCCTGAATGGGAGATGGCTTTTCGTAACCCAGATCGCTAAGGGCTCCAAGGATAGGAGCCTTCAGAccc                          >  2:390500/1‑143 (MQ=255)
                                                                                                                     tgcAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGTGGTGTATGTGGTTTTTCATAACCCAAATCGTTAAGGGCTTCAAGGATAGGACCCTTCAGGcaca                         >  2:546415/1‑143 (MQ=255)
                                                                                                                     ggcAGTTTTTCCGCGCCCCCTCTGTGCCATTCCCAGAACGGCGCGGCCAGTCAGTAGATTTGGACTACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGGCCCa                         <  2:587967/142‑1 (MQ=255)
                                                                                                                                cGCCCCCCGTCCGGGCCACACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGGCCCAGATCTGCaaaa              <  1:332968/143‑1 (MQ=255)
                                                                                                                                     ccattCTGGTCCATACCCAGAACGTCGCGGCTATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGGCCCAGATCTGCAAAAGTGGt         <  1:462702/139‑1 (MQ=255)
                                                                                                                                         tcctggCCATTCCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGGCCCAGATCTGCAAAAGTGGTTTCg     <  2:417789/139‑1 (MQ=255)
                                                                                                                                          ctttgCCATACCCAGAACGTCGCGGCCAGTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCCTCAAGGATAGGAGCCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGa    <  1:143940/139‑1 (MQ=255)
                                                                                                                                           tGGGCCATACCCATAAAGTCGCGGCCATTCAGTAGATGTTGAATACACTCTGCCTGAATTTGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTTAAGGATAGGAGCCTTCAGGCTCAGTTCTGCAAAAGTGGTTTCGaa   <  2:33210/143‑1 (MQ=255)
                                                                                                                                            gggCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAAt  <  1:180349/143‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                            
GAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGATTCTGCAACAGAGGTAAAGAGAATGCTGCAGTTTTTCCGCTCCCCGTCTGGGCCATACCCAGAACGTCGCGGCCATTCAGCAGATGTGGAATACACTCTGCCTGAATTGGAGATGGTTTTTCGTAACCCAGATCGTTAAGGGCTTCAAGGATAGGAGCCTTCAGGCCCAGATCTGCAAAAGTGGTTTCGAAT  >  NC_000913/3307570‑3307852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.