Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	4,001,645	Δ5 bp	coding (220‑224/951 nt)	corA →	magnesium/nickel/cobalt transporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	4001645	4001649	5	30 [0]	[0] 29	corA	magnesium/nickel/cobalt transporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 4001644	0 (0.000)	29 (0.760)	25/284	0.5	100%	coding (219/951 nt)	corA	magnesium/nickel/cobalt transporter
	?	NC_000913	4001650 =	0 (0.000)					coding (225/951 nt)	corA	magnesium/nickel/cobalt transporter

GATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCG  >  NC_000913/4001513‑4001784                                                                                                                                          |                                                                                                                                       tgagtgatcttgtcgaatcggactacgactagcgactgcgtgtacaatctaaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCctt                                                                                                                                   <  1:544168‑M2/6‑1 (MQ=255)     tgatcgtgccgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttc                                                                                                                               <  1:665687‑M2/10‑1 (MQ=255)          ttgtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCcttcttcttctt                                                                                                                          >  1:659076‑M2/129‑143 (MQ=255)           tgtcggaccggacgacgattagcgactgcgcgtataatttgatcttggtcatagggtggcaacccgccgggaacgggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCttt                                                                                                                         <  1:33613‑M2/16‑1 (MQ=255)            gtcgaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTg                                                                                                                        <  2:599641‑M2/17‑1 (MQ=255)               gaaccggacgacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggcactggccgtcatcgatgcatcggcgcgtttctttgaagacgaaaacggcataaatATCCTTCTTCTTAATTGAAg                                                                                                                     >  1:237527‑M2/124‑143 (MQ=255)                  ctggacgacgacgagtgactgcgcgtacattctgaacttggccagagcctggcaacccgcccggaacgggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATg                                                                                                                  <  1:231721‑M2/23‑1 (MQ=255)                        gacgacgagcgactgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAg                                                                                                            >  1:593284‑M2/115‑143 (MQ=255)                                     tgtgtgtacaatctgaacttggtcggagcctggcaacccgccgggaactggaagacgacgaagcatcggcacggttcggtgaagacgacgacggcctgcatCGCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTaa                                                                                               <  1:82874‑M2/42‑1 (MQ=255)                                     tgcgcgtacaatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgactacgtcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTaa                                                                                               >  1:206987‑M2/102‑143 (MQ=255)                                              aatctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACtgt                                                                                      >  2:36493‑M2/93‑143 (MQ=255)                                                tctgaacttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaaggcgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGCAGCGGCAGAACACGCCGGTAACTCCACTGTgg                                                                                    >  2:434901‑M2/91‑143 (MQ=255)                                                       ttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgcagacgactacggcctgcatCTCCTTCTTCTTCTTTGATGATACGGAAGATCACGCAGGTAACTCAACTAATGCATTTAc                                                                             >  1:629646‑M2/84‑143 (MQ=255)                                                       ttggccagagcctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgacgacgacgacggcctgcctCTCCTTCCTCTTCTTTGCAGATGCGTAAGATCACGCCGGTAACAACAATGTGGAATATAc                                                                             >  1:482104‑M2/84‑143 (MQ=255)                                                                 cctggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGAt                                                                   <  2:380479‑M2/70‑1 (MQ=255)                                                                    ggcaacccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAAATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGgt                                                                >  1:571596‑M2/71‑143 (MQ=255)                                                                          ccgcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGCGGCATTTACCATCCGTGCtggtcgtccg                                                          >  2:649502‑M2/65‑141 (MQ=255)                                                                            gccgcggactggaagagagcgaagcagcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGtt                                                        <  1:289674‑M2/81‑1 (MQ=255)                                                                            gcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGtt                                                        >  1:143178‑M2/63‑143 (MQ=255)                                                                            gcccggaactggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACACCGGTAACTACACTGTGGCATTTACCATCCGTGATGGTCGTCCGtt                                                        >  1:391955‑M2/63‑143 (MQ=255)                                                                                     tggaagacatcgaagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCAAGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGATTACTCTgcg                                               >  2:684723‑M2/54‑143 (MQ=255)                                                                                               cgaagcatgggcaggtttcttctaacacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCATGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGaa                                     <  2:279830‑M2/100‑1 (MQ=255)                                                                                                 aagcatcggcacgtttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAGGATCACGCCGGTAACTCCACTGTGGCATTTAGCATACGTGATGGTCGGATGTTTACTCTGCGTGAGCGTGAACt                                   >  1:612299‑M2/42‑143 (MQ=255)                                                                                                               ttctttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTGTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTc                     >  2:686263‑M2/28‑143 (MQ=255)                                                                                                                  tttgaagacgacgacggcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTCTTCGCCTGt                  >  1:691166‑M2/25‑143 (MQ=255)                                                                                                                        gacgacgacggcctgcatCTTCTTCTGCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCCTTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTa            <  2:329843‑M2/125‑1 (MQ=255)                                                                                                                        gacgacgacggcctgcatCTCCTTCTTCTTCTTTGAATATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTa            <  2:10833‑M2/125‑1 (MQ=255)                                                                                                                                  gcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTCTCGTATGCGTGCCCg  >  1:390569‑M2/9‑143 (MQ=255)                                                                                                                                  gcctgcatCTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATACGTGATGGGCGTCTGTTTACTCCGCGTGAGCGTGAACTGCCCGCTTTTCTTCGGTACTGTGTGCGtgccag  >  2:34041‑M2/9‑141 (MQ=255)                                                                                                                                          |                                                                                                                                       GATCTTGTCGAACCGGACGACGACGAGCGACTGCGCGTACAATCTGAACTTGGCCAGAGCCTGGCAACCCGCCCGGAACTGGAAGACATCGAAGCATCGGCACGTTTCTTTGAAGACGACGACGGCCTGCATATTCACTCCTTCTTCTTCTTTGAAGATGCGGAAGATCACGCCGGTAACTCCACTGTGGCATTTACCATCCGTGATGGTCGTCTGTTTACTCTGCGTGAGCGTGAACTGCCCGCTTTTCGTCTGTATCGTATGCGTGCCCG  >  NC_000913/4001513‑4001784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑