| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,060 | 0 | C | T | 34.5% | 38.1 / 39.3 | 56 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base C (20/16); new base T (8/11); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAT > NC_000913/4295920‑4296201 | aGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATATTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGc < 2:583975/143‑1 (MQ=17) tttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCACTGCCTCAt > 1:273208/1‑143 (MQ=12) gttACGCCCCATCCGACATCAACTCCTGATGCGGCGACTAACGCGTCTCATCAGGCCTACGCCAGACAGCGAACTAGCCTGATTTAGCGTG‑ATTTTGTCGGTCGGATAAGGCGTTTCCGCCGCATCCGACATCAACGCCTGAt < 2:89492/142‑1 (MQ=11) ttATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATg < 1:477956/143‑1 (MQ=32) ctATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATg < 2:271347/142‑1 (MQ=12) gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAATGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt > 1:352418/1‑143 (MQ=17) gCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCt > 1:485460/1‑143 (MQ=32) cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCtt < 1:702933/143‑1 (MQ=17) aCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAGTCGACGCTTGCcgcg > 2:715367/1‑143 (MQ=255) cATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGt < 1:418817/143‑1 (MQ=38) aTCACCGCCTGATGCGACGCTTATCGCGTCTTATCAGGCCTGCGCCCGACAGCGCACTAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc < 2:485460/143‑1 (MQ=11) aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc > 1:402930/1‑143 (MQ=25) aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTGGCCGCGTc > 1:254141/1‑143 (MQ=25) aaCGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGATTACGCCACATCCGACATAAAAGCATGATGCGACAATTGACGCGTATTa > 1:201186/1‑143 (MQ=11) gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa < 1:535918/143‑1 (MQ=39) gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCa < 1:535285/143‑1 (MQ=38) tgttGCGACGCTTGACGCTTCATATCGGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGATATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 2:207903/140‑1 (MQ=11) gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTCGGTCGGATAATGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCATGcc > 1:541106/1‑143 (MQ=255) gATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCCTGCCGCGGCTTATCACGcc > 2:393850/1‑143 (MQ=255) gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAGGGCGTTTACGCCCCATCCGACATCAACGCCTGCTGCGACGCCTTGCGCGTCTTATCCGGCCtccac > 1:207903/1‑139 (MQ=11) gACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct > 2:214329/1‑140 (MQ=17) cTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGa < 1:650007/143‑1 (MQ=38) aCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAccag > 2:723262/1‑140 (MQ=37) cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAgcg < 1:599682/143‑1 (MQ=39) cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGCCGTTTACGCCGCATCCGACATCAACGCATGCTGATGCGGTTTCCGCGTCTTATCACGCCTACCCACGACCGCGCa > 2:709173/1‑143 (MQ=11) tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc > 2:685457/1‑115 (MQ=37) tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTc < 1:685457/115‑1 (MQ=37) aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc > 1:249572/1‑120 (MQ=17) aTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc < 2:249572/120‑1 (MQ=37) atgCCCACGCCCGACAGCGCAATAGCCTGATTTCGCGTC‑ATTTTGTAGAGCGGATAAGGCGTTTACGCCGCATCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGa < 2:496130/141‑1 (MQ=14) aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGa < 2:274237/90‑1 (MQ=25) aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGa > 1:274237/1‑90 (MQ=35) tACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAg > 2:449026/1‑125 (MQ=21) tACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAg < 1:449026/125‑1 (MQ=40) aCGCCAGCCAGCGCACTGCCCTGATTCAGCGTG‑AATTTGTGGGGCGGCTAAGGCGTTCACGCCTCATCCTGCGTGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGc < 2:250040/143‑1 (MQ=14) ccAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGTCGCTTGCCGCGTCTTCTCAGGCCTACGCCATCTCGCGCCTTAGCTTGATCTACCGTg > 1:72241/1‑143 (MQ=11) aCAGCGCAATAGCCTGATCCACCGTGAAGCTAGTTGG‑CCGATATGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCGTGCCGCGTCATATCAGGCCTACGCCGACCAGCGCAATAGCCTGATTTCGCGTCAttt > 2:718237/1‑143 (MQ=2) gcgcAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCGGGCCTACGCCAGACAGCGCAAGAGCCCGCTTTAGCGTGATTTTGt > 1:2316/1‑143 (MQ=255) aaTAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCaa < 1:23242/115‑1 (MQ=40) tAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTCTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCg < 1:436813/143‑1 (MQ=255) tGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCt < 2:87117/61‑1 (MQ=255) tGATTTAGCGTG‑ATTTTGTAGGGCGGATAAGGCGTTTACGCCGCAGCCGACATGAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa < 1:579601/143‑1 (MQ=255) gctttCGCGTG‑ATTTTTTGGGGCGGATAGGGCGTATCCGCCGCGTCAGGCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg < 2:298798/141‑1 (MQ=11) gTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGAt < 1:15712/55‑1 (MQ=255) gTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAt > 2:15712/1‑55 (MQ=38) ttttGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACACCAACGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg > 1:271347/1‑143 (MQ=255) tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGCTTTTGTAGGTCGGATACGGCGCCTACGCCGCATct > 1:140242/1‑142 (MQ=11) aGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAACGCGTCTGTTCCGCCTctg > 1:323352/1‑141 (MQ=14) ggTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt < 1:706055/72‑1 (MQ=255) ggTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCt > 2:706055/1‑72 (MQ=255) gATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGAGGCGACGCTTGCCGCGGCTTATCAGGCCTACCCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGGTTATGGCGCATCCGGCATcg > 2:88438/1‑142 (MQ=14) aaaGGCGTTTACGCCCCATCCGACATCAACGCCTGATACGACGCTTGCCACGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAc < 1:319186/142‑1 (MQ=255) cgcATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGCTGCGTCGc > 1:388223/1‑143 (MQ=25) gtgacGACATCAACGCCTGAGGCGACGCTTGCCGCGTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCtt < 2:726531/139‑1 (MQ=255) cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAACGTGATTTTGTAGGTCGGATAAGGCGGTTATGCCTCATTCGACATCAACGCCTGACGCGACGCTTAGAGCGt > 2:97857/1‑143 (MQ=11) aCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTAt > 2:165320/1‑143 (MQ=14) | AGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAT > NC_000913/4295920‑4296201 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |