Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,184,543 C→A P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,184,5430CA94.7% 89.0 / NA 40P1100Q (CCG→CAG) rpoBRNA polymerase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base A (15/21);  total (18/22)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC  >  NC_000913/4184406‑4184676
                                                                                                                                         |                                                                                                                                     
tgCGGTTTAACGCCGTTTCCAGTCTGGTGACAAGATGGTAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTgg                                                                                                                                  <  1:485033/142‑1 (MQ=255)
        aaCGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACACCGGAAGACATAGTACTGAAACAGCTGGGCTTAcag                                                                                                                          >  1:1154/1‑141 (MQ=255)
          cGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCACCCCGATCGAAGATATGCCTTACGCTGAAAACCGTACGCCGGTGCACATCGTACTGCCCCCGCTCCGCGTCCCGtc                                                                                                                        >  1:76052/1‑143 (MQ=255)
              gTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGt                                                                                                                    <  2:69555/143‑1 (MQ=255)
               gatgcAGCCGGGGGACAAGAGGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAGCGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTa                                                                                                                   <  2:245437/139‑1 (MQ=255)
                    aGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGCTGTGCCTTACGCTGAAAACGGTACGCCGGTAGACATCGGACTGAACCTGCTGGGCGTACCGTCTCGTATGAAc                                                                                                              >  1:355561/1‑143 (MQ=255)
                     gCCTGGTGACAAGATGGCAGGTCATCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACCGTCCGACGGTCGACCTCGTCCTGCACCCGCTGGGCGCACCGCCTCCTATGAAcc                                                                                                             >  2:129326/1‑142 (MQ=255)
                                 gctgtgaGGTCGTCACGGTAACAAGGGTGTAATTGCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATcc                                                                                                 <  2:205795/137‑1 (MQ=255)
                                  aTGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGCACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCt                                                                                                >  1:469234/1‑143 (MQ=255)
                                       agctcctcACGGCAACAAGGGTGTAATTTCTCAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                           <  1:243452/137‑1 (MQ=255)
                                       aGGTCGTCACGGTAACAAGGGGGGAATTTCTAAGATCAACCCGATCGAGTATATGCCGTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGaaa                                                                                           <  2:114387/143‑1 (MQ=255)
                                         gtcgtcACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGCCTCGTATAAAACTCGGTGAGATCCCCGAAAcc                                                                                         >  2:330595/1‑143 (MQ=255)
                                              cACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAATACGGTACGCTGGTAGTCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCt                                                                                    <  1:67419/143‑1 (MQ=255)
                                               aCGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGCGCGTACCGTCTCGTATGAACATCGGACAGATCCTCGAAACCCaacag                                                                                   >  1:494186/1‑139 (MQ=255)
                                                 ggTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGCACACCGGTCAGATCCTCGAAACCCACCAggg                                                                                 >  1:621601/1‑143 (MQ=255)
                                                  atAACCAGGGTGTAACTGCGAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTTCGCCGGTAGACAGCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGt                                                                                <  2:207291/142‑1 (MQ=255)
                                                    aagaaGGGTGGAATTTCTAAGAGCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTAt                                                                              <  1:703989/140‑1 (MQ=255)
                                                            tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGCAACCCACCTGGGGATCGCTGCGa                                                                      >  1:244632/1‑143 (MQ=255)
                                                            tgtAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGa                                                                      >  1:511917/1‑143 (MQ=255)
                                                              ttaTTTTTAATATCTTCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGaaa                                                                    <  1:634549/141‑1 (MQ=255)
                                                               aaTTTCTAAGATCAACCTTATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                   <  1:42700/143‑1 (MQ=255)
                                                               aaTTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCTGTAGACATTGTTTTGGTCGAGCAGAGCGAACCGGCACGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAg                                                                   <  1:268495/143‑1 (MQ=255)
                                                                 tttCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTACGAAAGGt                                                                 >  2:1784/1‑143 (MQ=255)
                                                                   tCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCACCTGGGTATAGCTGCAAAAGatat                                                               >  1:680312/1‑143 (MQ=255)
                                                                    cTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCACGGATGAACATCGGTCAGATCCTCGAAACCCACCTCGGTATGGGTGCGAAAGGTATc                                                              >  2:336531/1‑143 (MQ=255)
                                                                     tAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGCCATCGTACTGAACCAGCTGGGCGTACCGTCTCGTCTGAACCTCGGTCAAATCCTCGAAACCCACCTGGGTATGGCTGCGAAAAGTATCg                                                             >  1:473127/1‑143 (MQ=255)
                                                                     tAAGATCAACCCGATCGAAGATATGCCTTACGAGGAAAACGGTACGCCGGTAGACATCGTACCGAACCAGCCGGGCGTACAGACTCGTATGAACATCGGTCAGGTCCACGAAACCCCCCTCGGTACTGCTGCGAACGGTCTCg                                                             >  2:549395/1‑143 (MQ=255)
                                                                          tCAATCCGATAGAAGATATGCCTTACGATGACAACGGTACGCCGGTAGACATCGTACTGAACTAGCTGTGCGTACCGTCTCCTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGAc                                                        <  2:205033/143‑1 (MQ=255)
                                                                            aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTGAGATCCTCGAAGCCCACCTGGGGATGCCTGCGAAAGGTATCGGCGActa                                                      >  2:373838/1‑141 (MQ=255)
                                                                            aaCCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACAAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGagca                                                      >  2:262476/1‑140 (MQ=255)
                                                                               ccGATCGAAGATATGCCTTACGATGAGAACGGTGCGCCGGTAGACATCGTACTGGACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGAt                                                   <  2:715147/143‑1 (MQ=255)
                                                                                   tCGAAGATATGCCTTACGATGAAAACGGTACGCTGGTAGACATCGTACTGTACTAGTTGGGCGTAGCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                               <  1:356695/143‑1 (MQ=255)
                                                                                   tCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACAGCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAAc                                               <  1:112177/143‑1 (MQ=255)
                                                                                             gCCGTACGATGAAAACGGCACGCCGGTAGACATCGAACTGGACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGa                                     <  2:42846/143‑1 (MQ=255)
                                                                                                               tACGCCGGGAGACATCGTACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAACCCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTcg                   <  2:365935/143‑1 (MQ=255)
                                                                                                                       tAGTCATCGTACGGAACCAGTTGGGTGTACCGTCTCGTATGAACGTCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTg           <  2:638324/143‑1 (MQ=255)
                                                                                                                             tcccaCTGACCCGGCTGGGCGTGCCGTCTCGTATGAACAGCGGTCAGATCCTCGAAGCCCGCCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGaa     <  2:621601/139‑1 (MQ=255)
                                                                                                                              cGTACTGAACCAGCTGGGCGTACCGTCTCGTGTGGCCAGCACACAAAGCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAAt    <  1:689874/143‑1 (MQ=255)
                                                                                                                                tACTGAACCAGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCGGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCACCGCCGTGGGGAAACAGCGGCAAGACGTCGCTCAACTGTGCGAGtta  >  1:3404/1‑142 (MQ=255)
                                                                                                                                caCTGAACCATCTGTGCGTACCGTCTCTTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTc  <  1:405329/142‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
GGCGGTTAAACGCCGTATCCAGCCTGGTGACAAGATGGCAGGTCGTCACGGTAACAAGGGTGTAATTTCTAAGATCAACCCGATCGAAGATATGCCTTACGATGAAAACGGTACGCCGGTAGACATCGTACTGAACCCGCTGGGCGTACCGTCTCGTATGAACATCGGTCAGATCCTCGAAACCCACCTGGGTATGGCTGCGAAAGGTATCGGCGACAAGATCAACGCCATGCTGAAACAGCAGCAAGAAGTCGCGAAACTGCGCGAATTC  >  NC_000913/4184406‑4184676

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑