New junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 3946824 | 8 (0.120) | 6 (0.090) | 5/280 | 9.4 | 15.2% | intergenic (+5/‑48) | rrfC/aspT | 5S ribosomal RNA of rrnC operon/tRNA‑Asp |
? | NC_000913 | = 4040681 | 59 (0.870) | intergenic (+45/+225) | rrfA/mobB | 5S ribosomal RNA of rrnA operon/molybdopterin‑guanine dinucleotide biosynthesis protein B | |||||
Rejected: Coverage evenness skew score above cutoff. | |||||||||||
Rejected: Frequency below/above cutoff threshold. |
GTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTG > NC_000913/4040540‑4040796 | gtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTa < 1:196953/143‑1 (MQ=35) ccACCTGACCCCATGCCGAACTCAGACGTGAAACGCCGTAGCGCCGATGGTAGCGTGGGGTATCCTCATGCGATGGTAGGGAACTGCCAGGCATCAAATAAACCGAAAGGCTCAGACGGCAGACTGAGCCTTTCGTTGTAGCt > 2:182906/1‑143 (MQ=11) tGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACGGCCAGGCATCAAATAATACGAAAGGCTCAGTCGGAAGACTTGGCCTTTCGTTTTATCtgttgt < 1:558430/143‑1 (MQ=255) tGACCCCATGCCGAACTCAGAAGCGAAACGCCGCAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCtgttgt < 1:42165/143‑1 (MQ=25) ccGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGATCAGTCGGAAGACTGGGCCTTTCGCTTTATCTGTTGTTTGTCGGTGa > 2:550714/1‑143 (MQ=21) tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGc‑tc > 2:117405/1‑143 (MQ=35) tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGc‑tc > 1:641651/1‑143 (MQ=35) tCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGc‑tc > 1:927161/1‑143 (MQ=35) aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGTACGC‑tctc > 1:27892/1‑143 (MQ=21) aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAGCGC‑tctc > 1:236663/1‑143 (MQ=25) aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTGTTATCTGTTGTTTGTCGGTGAACGC‑tctc > 1:661925/1‑143 (MQ=25) aGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTAGTTTTATAAGTTGTTTGTCGGTGAACGC‑tctc > 1:379377/1‑143 (MQ=255) atgTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGTATCTAATAAAACGAAAGGTTCTGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCt < 2:1089898/141‑1 (MQ=255) aaGTGAATCGCCGTTGCTCAGATGGTGGTGTGGCGTCTCCTGATGCGAGAGTAGGGAACTGCCAGGCATCAGATATAATGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCt < 2:897330/143‑1 (MQ=11) aaGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCt > 1:602676/1‑143 (MQ=35) tgggaCGCCGGAGCGGCGAGGGTAGTGTAGGGTCTCCTCGGGCGGGAGTAGGGACCTGCGAGGCATCAACTGAAACGAGAGGCTCAGTCGGAAGACTGGGCCTTTCGTGTTATCTGTGGTTTGTCGGTGAACGC‑TCTCCTGAg < 2:210419/139‑1 (MQ=11) tGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTgtg > 2:99420/1‑141 (MQ=25) tGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAg > 2:174598/1‑143 (MQ=35) gcacccccGCAGCGCCGTTCGCAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAACACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGt < 2:317256/137‑1 (MQ=11) ataaCGCCTTAGGTTCGATGTTAGTGTATGGGCTCATCAAGTGAGAGTAGGGAACTGCCAGGCATCAAATAAACGGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGt < 1:281272/141‑1 (MQ=11) aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAGCGC‑TCTCCTGAgca > 1:201026/1‑141 (MQ=25) aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAAATAAAAGGCGGCGATATATACGAAAGGCTCAGTCGGAAGACTCGGCCTTTAGATTTATCCGTTCTCTGTCGACGAACGC‑TACCCTGAgca > 2:500640/1‑141 (MQ=2) aCGCCGTAGCGCCGGTGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAACGGCTCAGTCGGAAGACTGGGCCTTTCGTGTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAgg < 1:1059759/143‑1 (MQ=255) aCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAgg > 2:660045/1‑143 (MQ=34) gCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAGAACGAAAGGCTCAGTGGGAAGACTGGGCCTTTCGTTTTGTCGGTTGTTTGTCGGTG‑ACGCGTCTCCTGGGGAGGAc > 1:620827/1‑143 (MQ=11) tAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAAt < 1:396613/143‑1 (MQ=35) tAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAAt > 1:374691/1‑143 (MQ=35) cGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATccgccg < 1:254834/143‑1 (MQ=35) cGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTAGGTGAACGC‑TCTCCTGAGTCGGACAAATccgacg > 1:597947/1‑143 (MQ=255) cGATGGTAGTGTGGGGTCTCCTCATGCGAGAGAAGGGAACTGCCAGGCGTCAAATAAAACGAAGGGCTCAGTCGGAAGACTGGGCCTATCGTTTTATCTGTTGTTTGTCGCTGAATGA‑CCTCCGTAGTATGTCAAATgcgccg > 2:178596/1‑143 (MQ=11) ttgTAGTGTGGGCCCTCCTGATGCGAGAGTAGGGAACCGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGa < 2:230437/141‑1 (MQ=255) tgGTGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGAttt < 1:56256/143‑1 (MQ=25) gggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACACATCCGCCGGGCGCGGATTTGa > 2:177744/1‑143 (MQ=25) gggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGa > 1:778200/1‑143 (MQ=35) ggTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTGTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGGCAAATCCGCCGGGAGCGGATTGGaa > 1:830546/1‑143 (MQ=255) gTCTCTTCATGCGATAGTAGGTAACTGCCAGGGATCAAATAAAACGAGAGGCTCAGTCGGAAGACTGTGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAAc < 2:482894/143‑1 (MQ=11) ctcATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGc > 2:326630/1‑143 (MQ=33) aTGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTTTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGaa < 1:174598/143‑1 (MQ=17) aTGCGAAAGTAGGGAACGGCCAGGCATAAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGATTTATATGTTGTTTGTCGGTGAACGC‑TCTCCTCAGTAGGCCAAATCCGCCGGGAGCGTATTTGAACGTTGCGaa < 1:984880/143‑1 (MQ=11) tggggAACCGCCGGGAGTCAAATAGAATGAGGGGCTCAGGCTGAAGACTGGGCCTTTCGTTTTATCTGCTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGcc < 2:594936/141‑1 (MQ=11) tAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGcc > 2:206489/1‑143 (MQ=32) gggAACGGCCAGGCAGCAACTAAAACGGGAGGCTCAGTGGGAAGCCTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATACGCCAGTAGCGTATTTGAACGTTGCGAAGCAACGGCCCg < 2:689776/143‑1 (MQ=11) ggAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCgg > 1:407320/1‑143 (MQ=32) ggAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGCAGCGCATTTGAACGTTGAGAAGCAACAGCCCgg > 2:411790/1‑143 (MQ=255) gAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCCCCTGGAGAGGCCAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCCGa > 2:167287/1‑143 (MQ=11) aCTGCCAGGCATCAAATAAAACGAAAGGCTCGGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCAGGacg > 2:1200215/1‑141 (MQ=12) tGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAgggg > 2:903235/1‑142 (MQ=17) tGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATCTGACCGTTGCGAAGCAACGGCACGGCgggc > 2:556926/1‑142 (MQ=255) gCCAGGCATCACATAAGACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑GCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGTCCGGAGGGTg < 2:676977/143‑1 (MQ=255) cAGGCATGAAATAATACGAAAGTGTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGc < 2:93820/143‑1 (MQ=255) gtcATCAAGTAATACTAAAGGCTCAGTCGGGAGACTGGGCCGTTCGTTTTAGCTGTGGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATTCGCCGGGAGCGGATTTTAACGTTGCGAAGCAACGGCCCGGAGGGTGGCgg < 1:356610/141‑1 (MQ=11) gtcATCAAATAAAACGAAATGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCgg < 1:356829/141‑1 (MQ=17) cATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGCGCGGAGTTGAACGTTGCGAAGCCACGGCCCGGAGGGTGGCgggg > 1:997278/1‑142 (MQ=12) tCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGCGGCGGGCAg > 1:538838/1‑143 (MQ=17) gtAAAGCGAAAGGCTCAGTCGGGAGACTGGGCCTTTCGTTGTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAcg < 1:507534/142‑1 (MQ=12) aaaCGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAcgccc > 2:133935/1‑143 (MQ=32) aaaCGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGAcgccc > 2:962905/1‑143 (MQ=32) ggCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGCGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGGGGCGGGCAGGACGCCCGGCagaca > 2:1125294/1‑139 (MQ=255) gCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAAc > 1:694466/1‑143 (MQ=32) tCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGGAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCGGGACACCCGCCATaacccg > 2:728234/1‑139 (MQ=255) | GTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGGAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGC‑TCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTG > NC_000913/4040540‑4040796 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
Reads not counted as support for junction |
read_name Not counted due to insufficient overlap past the breakpoint. |
read_name Not counted due to not crossing MOB target site duplication. |