Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	144.3 / NA	52	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/24);  total (28/24)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGA  >  NC_000913/2132650‑2132919                                                                                                                                          |                                                                                                                                     tCTGGTAGAGGGTAAACAGCTGACATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                 <  2:1098290/143‑1 (MQ=255) tCTGGTAGAGGGGAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACATGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                 >  1:1040435/1‑143 (MQ=255)   tGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATACGCAGGCTCTGCGt                                                                                                                               <  1:324941/143‑1 (MQ=255)    gggagagGGTGAACAGCTGATATTTTCGGCGGGCGCGGTCGAATTGGCCTTTGTGCTTGCGGGAAAAGTGGATGGACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                              <  1:392463/140‑1 (MQ=255)      tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                            <  1:710625/143‑1 (MQ=255)        gagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCCTGATTGATACGCCGGCTCTGCGTCGCct                                                                                                                          >  1:970328/1‑143 (MQ=255)             tAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTGTGGGTCGcttcttat                                                                                                                     >  2:282798/1‑141 (MQ=255)               aaCAGCTGATATTTTTTGCTGGCGCGATCGAATTTGTCTTTGCGCTTGCGGTAAAAGTGGAAGTGCCGGGAGTAGTTTTTCGGTGCCGAGGTGATCTGACTTTCACCGTGATTGATATACAAGCTCTGGGTCGCCtcctctcc                                                                                                                   >  2:606188/1‑141 (MQ=255)                aCAGCTGATATTTTTTGCAGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACAGTGATTGCTATGTAGGCTCTGCGTCGCGTCTTGTAcg                                                                                                                  >  2:136536/1‑142 (MQ=255)                     tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATGTGCGTTTCACGGTGATTGATATGCCGGCTCTGCGTGGACTCTCCTACTTGccg                                                                                                             >  1:842306/1‑142 (MQ=255)                         atTTTTTGCTGGCGCGGTCGAATGTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGt                                                                                                         <  2:825149/143‑1 (MQ=255)                          ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATGTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACCGtt                                                                                                        >  2:1232396/1‑143 (MQ=255)                          ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                        >  1:1049194/1‑143 (MQ=255)                          ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                        >  1:1036263/1‑143 (MQ=255)                           tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCGTCTTCTACTTTCCACGGTTc                                                                                                       >  2:1036406/1‑143 (MQ=255)                              ttGCTGGCGCGGTTGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                    <  1:277381/143‑1 (MQ=255)                                gCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCCGTAAAAGTGGAAGTACCCAGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGCTCGccct                                                                                                  >  2:656495/1‑141 (MQ=255)                                       gggTCGCATGTGTCGTTGGGTGTGCGGTAAAGGGGGACGTAGCCGGAGAATGTTTGAGGTGGCGAGGTGGTGTGCATTTCGCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                           <  2:640341/142‑1 (MQ=255)                                             agttgttCTTTGGGCGTGCGGTAGAAGTGGACGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTTCTTTCCACGGTTCGCCGTACTCCACCACCAt                                                                                     <  2:52397/137‑1 (MQ=255)                                                      ttttgCTTGCGTTAAAAGCGCAGGTACTCGGAGAATTGTTTCGGTGACGAGGTGATCTTCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                            <  2:1105822/140‑1 (MQ=255)                                                           cTTGCGTGAAAAGTTGACGTACCCGGTGCATCTTTTCAGTGAGGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGt                                                                       <  2:1209183/143‑1 (MQ=255)                                                            ttGCGGTAAACGTGGACGTCCCCGGGGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTTGCCGTACTCCACCACCATCCGCAGGAAGATGTc                                                                      <  2:1117362/143‑1 (MQ=255)                                                                ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGCAAGATGTCGTaa                                                                  >  1:608952/1‑143 (MQ=255)                                                                ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGAAGAGGTGATCTGCATTTCACCGTGATTGCTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTac                                                                  >  1:419241/1‑142 (MQ=255)                                                                  tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATc                                                                >  2:272313/1‑143 (MQ=255)                                                                   aaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTGCTCCACCGCCATCCGCAGGAAGCTGTCGtgagtc                                                               >  1:588930/1‑138 (MQ=255)                                                                       gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACAGTGCTGGCTTGTGATGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                           <  1:285379/143‑1 (MQ=255)                                                                                  cGGAGAATTTTTTCACTGACGAGCTGCTGTGCTGCGCACCGCGATTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAATCCTCTGCCGCTTTCAGt                                                >  2:271049/1‑143 (MQ=255)                                                                                   ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACTACCATCCGCAGGAAGATGTCGTAATCCTGTGCAGCTTTCAGtt                                               >  1:942202/1‑143 (MQ=255)                                                                                    gagaATTTTTTCGGTGAGGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGGGTCACCTCTTGTACTTTCCACGGTTCGCCGTACTCCACCACCGTGCGCCTGCAGATGTCGTAAGCCTGTGGGGCTGTCAGTTc                                              >  1:600059/1‑143 (MQ=255)                                                                                     aggtttttttCGGTGGCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                             <  2:786485/139‑1 (MQ=255)                                                                                          tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTTCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                        <  1:524311/143‑1 (MQ=255)                                                                                          tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTTCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTGTTCCGCTTTCAGTTCGTTATc                                        >  2:94914/1‑143 (MQ=255)                                                                                          tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                        <  1:573472/143‑1 (MQ=255)                                                                                               cGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGgaca                                   >  1:388779/1‑143 (MQ=255)                                                                                                    gcggggTGATGTGCATTTCACCGTGGTTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATGCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAacac                              <  2:539822/139‑1 (MQ=255)                                                                                                       aGGTGATCTGCATGTCACCGTGATTGATATGCAGGCTCTGCGTCGGCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActct                           <  1:57709/143‑1 (MQ=255)                                                                                                         gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt                         <  1:346900/143‑1 (MQ=255)                                                                                                            aTCTGCATTTCACCGTGATTGATATGCAGTCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAGTCCTCTGCCGCTTTCAGTTCGGTAAACAACAAACGctctgcgaa                      >  2:834693/1‑138 (MQ=255)                                                                                                            aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                      >  1:637334/1‑143 (MQ=255)                                                                                                             tCTGCATTTCCTCGCGAGTGATCTGCAGGCTCTGCGTCCCCTCTTCTACTTTCCACTGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                     <  2:552142/143‑1 (MQ=255)                                                                                                             tCTGCATTTCACCGTGATTGGTATGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCACGGTTCGCCGTACTCCACCACCAGCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGGTCGGTATCGAACAAACACTCTTTGaaa                     <  2:482381/143‑1 (MQ=255)                                                                                                              cttctgttCACCGAGATTACTATGCATGCTCTGCGTCTCCTCTCCTACTTTCGACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGGAATCTTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAc                    <  2:900609/137‑1 (MQ=255)                                                                                                               tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAGCCTGTGCCGCTTTCCGGTCGGTACCGAACAAACAGTCCTTgcacca                   >  2:33500/1‑138 (MQ=255)                                                                                                                gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACCCTCTTTGAAAccc                  >  1:213431/1‑141 (MQ=255)                                                                                                                       aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGCTCGGTATCGAACCAACACTCTTTGAAACACCATGccc           >  1:5991/1‑143 (MQ=255)                                                                                                                        ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCa          <  2:198922/143‑1 (MQ=255)                                                                                                                         cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg         >  2:250549/1‑143 (MQ=255)                                                                                                                          gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAgg        >  1:721976/1‑143 (MQ=255)                                                                                                                              ttgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGACCAAACACCCTTTGAAACGCCATGCCCAGGTaaa    >  2:163432/1‑143 (MQ=255)                                                                                                                               tgatAGGCAGGCTCTGCTTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAg   <  2:787765/143‑1 (MQ=255)                                                                                                                                gatATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGa  <  1:735089/143‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGA  >  NC_000913/2132650‑2132919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑