Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,560,455:1 | +G | 100% | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,560,455 | 1 | . | G | 88.9% | 169.9 / 11.8 | 63 | intergenic (‑2/+1) | glpR/glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
Reads supporting (aligned to +/- strand): ref base . (6/1); new base G (43/13); total (49/14) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
AACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAC > NC_000913/3560221‑3560682 | aaCGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GGGCCGCACCGCCAGGAT‑GG‑cgcag > 1:555423/1‑235 (MQ=255) aaCGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑cgcag > 1:73848/1‑235 (MQ=255) ttAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGGGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCCTGTT‑GGCC‑CGGGATCAg > 1:882649/1‑239 (MQ=255) aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGt > 1:356593/1‑239 (MQ=255) aCGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGtttt > 1:880747/1‑238 (MQ=255) ttGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGTTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGGGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGTCGGATCAGGTTTTGCt > 1:123613/1‑238 (MQ=255) ttGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGAAAGGCA‑GCGCCGCACCGCCATGAT‑GGCC‑CAGGACCAGGTTTTGCt > 1:775258/1‑238 (MQ=255) gttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCCCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGc > 1:539773/1‑238 (MQ=255) ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATTATGGG‑C‑AAGGACCAGGTTTTGCTCCGccgcccc > 1:519157/1‑234 (MQ=255) ttgGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGc > 1:323141/1‑237 (MQ=255) tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GGCC‑CAGGAACAGGTTTTGCTCCCCCACCTCCTTTGGGTc > 1:599541/1‑238 (MQ=255) tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GGAG‑CAGGACAAGGTTTTGCTCCGCCAGCTCATTGAGGTcg > 1:225850/1‑239 (MQ=255) tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTTAGGTcg > 1:370499/1‑239 (MQ=255) tGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc > 1:34622/1‑238 (MQ=255) gCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTTGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCCTGTC‑GG‑CGCAGGACCAGGCTTTGCTCCGCCAGCTCATTCAGGTCACGGCGaaaa > 1:356201/1‑236 (MQ=255) gCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGACCTGGAAGGCA‑GCGCCGCACCGCCCTGAT‑GGCC‑CAGGATCAGGTTTTGCCCCGCCCGCTCATTGGGGTccgcgcgaaaaa > 2:476904/1‑228 (MQ=255) gCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTTAGGTCCCGGCGAAAAg > 1:376113/1‑239 (MQ=255) tgtgATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCAGCGAATAg > 1:495698/1‑237 (MQ=255) gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCC‑CGGCGTGTTAACCGCACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGACCAGGTTTTGCGCCGCCATCTCACTACGGGCGCGGAGAATAGGACACGGGGTGACggg > 2:44013/1‑238 (MQ=255) gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCCGGATCAGGTTTGTGTCCGCCCGCCCCTTGAGGGCGCGCCGAATAGTCAGCGGGCTGACGga > 2:249189/1‑239 (MQ=255) gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAAAGTCTGCGGGCTGCCGga > 1:210134/1‑239 (MQ=255) gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGGTTTGGCCCCCCCGCCCATTTAGGCCCCGGCGAAAAGGCGTCGGGCGGAgggg > 2:82477/1‑238 (MQ=255) cgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGACCTGGAAGGCA‑GCGCCCCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCACCTCATTGAGGACCCGGCGAAAAGGATGGGGGCGGAggggg > 2:452074/1‑237 (MQ=255) aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCCCCGCCATGAG‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCGCGGCGAAAAGTCTGGTGGCGGGCGGAGAAAtaccc > 2:251408/1‑235 (MQ=255) cGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCG‑TCGCCGCACGGCCATGAT‑GG‑CGCCGGATCGGATTTTGCCCCGCCAGCTAATTAAGTTCGCGGCGAAGAGACCGCGGGCTGGCGGAGCAATGCCCTAcccaccctt > 2:596236/1‑232 (MQ=255) cGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCCGGTTTTGCTCCGCCAGCCCCTTGAGGTCGCGGGGGAAGGTCTGCGGGCTGACGGGGAAATGCTCTACCAGCTCtt > 2:400943/1‑239 (MQ=255) gtgCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTCCCAGCTCTTCGGt > 1:39985/1‑239 (MQ=255) aCAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTGTGCAGGCTGACGGGAAAATGCACTACCAACTCTTCGGTACTTACAAAACCctcg > 2:123529/1‑237 (MQ=255) gCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGCGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTGCTGACAAAACCctgctgg > 2:433104/1‑238 (MQ=255) cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGGGAAATGCTCTACCAGCCCTTCGGTACTGCCATAACCCTCCTGtt > 1:275089/1‑239 (MQ=255) cGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGGGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTTGGGGCGCGGCGAATAGACTGCGGGCTGTCGGAGACATTGTCTCCAAGCCCGTCGGTCATGGAATAACCCCGgtgta > 2:847728/1‑238 (MQ=255) tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGGGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCCTTACCAGCTCTTCGGTATTGACATAAcccgtcaggtta > 1:876820/1‑230 (MQ=255) tttgggggTTTTGCCCCGCCACTTTGCGGGCGGTGCGCTCTTTTTCGTCGGTCGGGGGGGCCTTGCGACCGGGCCC‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg < 1:859503/234‑1 (MQ=255) ttGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGATTAGTATGCGGGCTGACGGAGAAATGCGCTACCGGCTCTTCGGTCCTGCACTAACCCTGCGGTTTAAcaggttg > 2:96347/1‑233 (MQ=255) tGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGCGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCCCTTCGGAACTGACATAACCCTGCTGTTTAACCAGTTCGACAAAAcc > 1:488814/1‑239 (MQ=255) gccccGCCACTTTGCGGGCGATCCCGTTTTTTTTTTCGGTCTGGGTGGCCTTGCGATCGTCCCA‑CGGGGTGTTACCCGAACTGGAAGGCA‑GGGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCg < 1:569893/236‑1 (MQ=255) gCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTACGGGTTAACCAGTTCGATAATACCg > 1:844355/1‑239 (MQ=255) gcaccTTTGGGGGCGATGCCCTCTTTTTCCTCGTTCGGGGGGGCCTTGGCATCGGGCCC‑GGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgtg < 1:600726/235‑1 (MQ=255) gttttGGGGGCGATGCGCTTTTTTTCTTCGGTGTGGGTGGCCTTGCGATCGTCCCG‑CGGGGTTTTAACCAAACGGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACg < 1:491588/237‑1 (MQ=255) gATGCGCTTTTTTTCTGCGGTCGGGGTGGCCTTGCGATCGGGCCA‑CGGCGTGTTAACCGAACGGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttgt < 1:96347/238‑1 (MQ=255) gcgcTCTTTTTCTTCGGTCGGGGTGGCTTTGCGTGCGGGCAC‑GGGCGTGTTAACCGAACTGGAAGGCC‑GCGCGGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc < 1:858033/238‑1 (MQ=255) ttttgCTGCGGTGTGGGGGGCCTGGTG‑TCGGGCCAGCGGTGTTTGAACCG‑ACTTGGAGGCACCCGCCCCCCCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTtata < 2:39985/239‑1 (MQ=255) cGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTg > 1:239933/1‑239 (MQ=255) gTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGa > 1:655130/1‑239 (MQ=255) ccTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCg > 1:173645/1‑149 (MQ=255) cTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGCT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGGAATGCTCTACCAGCTCTTCGGGACTGACATAACCCTTCTGTTTAACCAATACGCAAAATCCGGTGGGACCTTTGGTTTTATTCATTTTAAAAATCCCGGAAatttttaccg > 2:45129/1‑235 (MQ=255) cTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTAttttcgt > 1:830330/1‑239 (MQ=255) cGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTAttttcgttttc > 1:180167/1‑239 (MQ=255) gTGCCA‑GGGTGTGTTAACCAAACGGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCa < 1:593303/239‑1 (MQ=255) gTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGCTAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCCGGAATTATTTTCTTTTTTGGGCa > 2:310965/1‑239 (MQ=255) a‑CGGGGTTTTAACCAAACGGGAAGGCA‑GGCCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATACCTGGAATTATTTTCGTTTTCGCGCATTGAg < 1:476904/239‑1 (MQ=255) tgtTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGTTTAACCAATTCGATAAAACCCTTCTGACGTTGTGTTTTTTTCC‑TTTTTAACTCCCTGGAATTATTTTTGTTTTTCCGCAtttcccgaacc > 2:120169/1‑230 (MQ=255) tgtTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGCATTATTTTCGTTTTCGCGCATTGAGCGATTc > 1:80296/1‑239 (MQ=255) gcggggaGGCC‑GCCCCGCCCCGCGATGGT‑GG‑CGCAGGGTCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc < 2:459438/233‑1 (MQ=255) aCTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTTTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTTGACCATTTACGGAATaaaaaaaaaca > 2:244161/1‑236 (MQ=255) aCTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACATTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTTGTTTTCGCGCATTGGGCTAATCAACAAAAGc > 2:447401/1‑238 (MQ=255) aGGCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATCCCGTTGTGAAGTTTGTTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTAAGCGAACAAACaaaaccaaccgca > 2:180441/1‑230 (MQ=255) ggCA‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCCTTTGGCGAATAAACAAAAACAATCGcaa > 2:620039/1‑237 (MQ=255) gCA‑GGGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCGGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt < 1:400943/237‑1 (MQ=255) a‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt < 1:167012/235‑1 (MQ=255) a‑GCGCCGCACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt > 2:167012/1‑235 (MQ=255) cgccgcACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAcc > 1:600161/1‑238 (MQ=255) cgccgcACCGCCATGAT‑GG‑CGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAc > 1:459438/1‑237 (MQ=255) ccGCCATGATGGG‑G‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa < 1:82477/239‑1 (MQ=255) gacATGAT‑GG‑CGCAGGATCAGGTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAc < 1:120169/237‑1 (MQ=255) | AACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCA‑CGGCGTGTTAACCGAACTGGAAGGCA‑GCGCCGCACCGCCATGAT‑GG‑C‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCA‑TTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAC > NC_000913/3560221‑3560682 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |