Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,590,065 | A→G | 18.2% | E824G (GAG→GGG) | acrD → | aminoglycoside/multidrug efflux system |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,590,065 | 0 | A | G | 18.2% | 143.8 / 3.9 | 67 | E824G (GAG→GGG) | acrD | aminoglycoside/multidrug efflux system |
Reads supporting (aligned to +/- strand): ref base A (23/29); new base G (11/4); total (34/33) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.72e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTA > NC_000913/2589833‑2590291 | acacTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGAttgt > 1:643167/1‑239 (MQ=255) acTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGAttgttg > 1:54364/1‑239 (MQ=255) acTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGAttgttg > 1:697399/1‑239 (MQ=255) tGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTCTTCTGCGG‑TTGGGATTGTTggg > 1:764676/1‑239 (MQ=255) tGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGGCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGTTTGTTggg > 1:435318/1‑239 (MQ=255) gCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGGGCCCTTCTCTGCTTTCGCGACCTCCCGCTGGGGAACAGG‑CTGCCCGCGTCTGGAAGCCCATAACGGTTTTTTTGGGG‑TTGGGGTTTTTGGGGAAGccc > 2:736728/1‑237 (MQ=255) ccTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGc > 1:790878/1‑238 (MQ=255) ggggTTTGGGTTTTGTGAATGACTTTTTGGATCGCGGTCGCGTGAAAAAAGTCTATGTGCGGGCAGCTGCGCCGTACCGCATGCTGCCGGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCg < 2:435318/239‑1 (MQ=255) ggggTTCGAGCTATGTGAATGGATTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTAGCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCg < 1:443053/239‑1 (MQ=255) gggTTCGGGTTTTTTGAATGTCTTTATGGATGGCGGTCGCGTGAAAAAAGTCTTTGTGCAGGCACCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCGTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCg < 2:591438/238‑1 (MQ=255) gggTTCGAGTTTTGTGAATGACTTTTTGGATCGGGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCg < 2:581401/238‑1 (MQ=255) ttcgtgtgtgtgAAGGACTTTAGGGATCGCGGGGGCGTGAAGAAAGTCTGTGTGCAGGCAGCTGGGCCGTATCGCATGCTGCCAGATGACATCAATCCCTGGTATGTCCGAAATAAAGAGGGCGGCAGGGTGCCCTTCTCTGGTTTCGCGACCTCCCGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGt < 2:774073/231‑1 (MQ=255) tCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGt < 1:579939/239‑1 (MQ=255) tCGAGCTATGTGAATGACTTTAGGGATCGGGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACCTCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGt < 1:361013/239‑1 (MQ=255) gCTATGTGAATGGCTTTAGGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGAATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAg < 1:533260/238‑1 (MQ=255) aTGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTa > 1:581401/1‑237 (MQ=255) tgtgAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCg > 1:4191/1‑239 (MQ=255) gtgAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAACTAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGATTGTTGGGGAAGCCGCCCCGGGGGTTCGTACCgg > 1:341343/1‑239 (MQ=255) gAATGACTTTTTGGATCCGGGTCGCGGTGAGAAAATCTTGTTGACGGCAGCCGCCCCGTTTCGCAGGCTGCCAGATGACAACAATCTCTGGTGTGTCCGAAATAAAGAGGGCGGCATGGTGCCCTTTTCTGCTTTCTCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTa < 2:544297/239‑1 (MQ=255) gAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTTCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTa < 1:876759/239‑1 (MQ=255) tttgtggtgtCGCGGTTGGGTGAAGGAAGTTTTTGGTAAGGCGGATGCGCCGTATCGCATGCTGCCAGATGACACCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTGCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCg < 2:535562/229‑1 (MQ=255) ttttgggttCGGGGTCGCGTGAATAAAGTTTATGTGAAGGCGGCTGCGCGGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGAt < 2:63872/231‑1 (MQ=255) tttATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAACCAGG‑CTCCCCGCGTCTGGAACGCTATACCGGTTATTCTGCGG‑TTGGGATTGTTGGGGAAGCGGCACCGGGGGCCAGGACAGGGACggg > 2:211370/1‑235 (MQ=255) tCGCGGTCCCGTGAAGAAAGGCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGTAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGAtatt < 1:27174/238‑1 (MQ=255) cgcgGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGATTGTGGGGGAAGCCGACCCGGGGGTCCGTACCGGCACGGCGATGGAttttat > 2:125655/1‑239 (MQ=255) cgcgGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCCGTACCGGTACGGCGATGGAtattat > 1:776575/1‑239 (MQ=255) cgcgGTCCGGTGAAAAAAGCTTTTGTGCGGGAAGCTGCGCCGTATCGCAGGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCCCGGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGAtattat < 2:357758/239‑1 (MQ=255) ggTCGCGTGAAGAAAGTCTATGTGGAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGa < 1:124073/239‑1 (MQ=255) gcgTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCCGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATc < 1:460676/238‑1 (MQ=255) tgtgCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCGGGAACCCTATAACGGTTCTTCTGCGG‑TTGGGATTGTTGGGGAAGCGGCACCGGGGGTCGGAACCGGTACGGGGAGGGAGATTTTGGAATAGGTAGTGAAGAAGGTGc > 2:777889/1‑239 (MQ=255) catggcgCTTCGCCGTTTCGCAGTGTGACAAATGACAACAATCTCTGGTATGTCCTAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGTAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGTTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCaaa < 2:597565/233‑1 (MQ=255) caggcatgggcgcGGAATCGAACGCTGCCAGATGACCTCAATCTGTGGTAGGGCCGAAATAAAGATGGCGGCAGGGGGCCCATCTTTGCTTTCGGGACCGCCCGCGGGGAAACAGG‑CTCGCCGCGTCTGGAACGGTATAACGGTTATTCTGCGG‑TTGAGAGTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCaaa < 2:822913/230‑1 (MQ=255) gcagCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTTCGG‑TTGGGATTGTTGGGGAAGCCGCACCGGGGGAGCGGACCGGTCAGGCGATGGGTATTTGTGAATCGTTTGAGAAGCAGCCGCCAAACg > 2:442949/1‑238 (MQ=255) cccctgttCGCCTGCTGCCAGATGACCTCAATCTCTGTTACGGCCGAAATAATGACGGCGGCAGGGCGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGt < 2:780238/232‑1 (MQ=255) aTGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGa < 1:736728/238‑1 (MQ=255) gctgcCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACgg < 1:777889/239‑1 (MQ=255) tgcCAGATGACATAAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCg < 1:207000/239‑1 (MQ=255) tGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGAt < 2:186601/158‑1 (MQ=255) tGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGGGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGAt > 1:186601/1‑158 (MQ=255) tGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGAAATCGTTAGTGACGCAGGTGCCAACGGGCTTTGGTGTGGGTGGGCCGGCAGTGTGGTATCAGGAGGGGCt > 2:763537/1‑239 (MQ=255) ggTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGCTTGGAGGGGACGGCGGTTTCGTATCAGGACCGGCtt > 1:385780/1‑239 (MQ=255) tGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGCTGGGTATTATGGAATCGTTAGTGAAGCAGCTGCCAAAAGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCCGGAGCGGCTTTCCg > 1:293966/1‑239 (MQ=255) gTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGCTATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCg > 1:35595/1‑238 (MQ=255) aaaTAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGAtatt < 1:546997/154‑1 (MQ=255) aaaTAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGAtatt > 2:546997/1‑154 (MQ=255) aaTAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGAGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCACACGGCTTTGGACTGGAGTGGACGGCGATTTGGTATAGGGAGGGGCTTTACGgggcgcc > 2:481076/1‑238 (MQ=255) gcggcATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTTTGGAGTGGACGGCGATGGCGGAACAGGAGAGGCTTTCCGGCGCGCAGGCCCGGgcg > 2:336931/1‑239 (MQ=255) tGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGTTCCGGCGCTGtttg > 1:758724/1‑234 (MQ=255) tgtCGCGACCCTACGTTGGGAAACCGGCCGCGCGGCG‑GTGGAACGCTTGCACGGTTATTCTGTGG‑TGGAGATGTTGGGGGAAGCCGCACCGGGGGTCAGTCCCGGTACGGCGATGGATTTTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGTTCCGTCGCTGTACGCCATTTCCTTGCtgg < 2:529589/237‑1 (MQ=255) tCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCCTTTCCTTGCtgggg > 1:119343/1‑237 (MQ=255) gcgACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTAACGGTCCGGCGATGGATATTTTGGAATCGTTAGTGAAGCAGCTTCCAAACGGCTTTGGGCTTGAGGGGAGGGCGAGGTCGAATCAGGAGCGGCTTTCCGGCGGGGGGGCACCGGGGCGGGCCGCCAATTCCtttgggggggg > 2:618243/1‑230 (MQ=255) gACCTCACGCTGGGAAGCGGG‑CTCGCCGCGTCGGGAACGCTATATCGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCTTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTa < 1:723449/238‑1 (MQ=255) cTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTTCGCCATTTCCTTGCTGGGGGGAtt > 1:134035/1‑237 (MQ=255) cTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATAAGGAGCGGCTTTCCGGCAACAAGGCTCCGGCGCTGTAGGCCATTTCCTTGCTGGTGGTCtt > 1:589535/1‑237 (MQ=255) ggAAACAGG‑CTCGCTCGGTGTGGAACCCTTTAACGGTTTTTTGGCGG‑TTGAGATTGTTGGGGAAGCCGCCCCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTgg < 2:854153/239‑1 (MQ=255) gAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTgg < 1:200040/238‑1 (MQ=255) gggtctgccgcGTCTGGAACGCTATAACGGTTATTCTGGGG‑TTGGGATTGTTGGGGAAGCCGGCCCGGGGGTCAGTTCCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGGCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGt < 2:119343/233‑1 (MQ=255) g‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGGATTCCTGGGTCTGGCTGCggtt > 1:756062/1‑236 (MQ=255) cgggccgcGTCGGGAACTGTATAAGTGTT‑TTCTGGGGTTTGGGATTGTGGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAAGCGGTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGGGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGt < 2:568912/233‑1 (MQ=255) tCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTa > 1:577139/1‑239 (MQ=255) ggAACGCTATAACGGTTAGTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGt < 1:211370/239‑1 (MQ=255) cTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGGTGTATGAAAGCTGGTCGGTg > 1:885136/1‑238 (MQ=255) caCGGTTATTGTGGGG‑GTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGtt < 2:54364/238‑1 (MQ=255) aaCGGTTATTCTGCGG‑TTGGGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGtt < 1:618243/239‑1 (MQ=255) cGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGGGTGGACGGCGAGGTCGTATCAGGAGCGGCTTTCCGGCGCCCGGGGCCCGACGCGTTACGCACTTTCCTTGCTGGTGGTCTTCCTGTGCTTGGCGGCGTGTTATCAAACCTGGTCGGTGCCGt > 2:619238/1‑236 (MQ=255) aTTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGGTGGCGGATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGGATTCCGGTGTCTGGCTGTGTTGTATGAAAGCTGGTCGGGGGCGTTCTCggga > 2:372315/1‑237 (MQ=255) aTTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTa > 1:825896/1‑239 (MQ=255) | ACACTGCAAACCGCCTGGGGTTCGAGCTATGTGAATGACTTTATGGATCGCGGTCGCGTGAAGAAAGTCTATGTGCAGGCAGCTGCGCCGTATCGCATGCTGCCAGATGACATCAATCTCTGGTATGTCCGAAATAAAGATGGCGGCATGGTGCCCTTCTCTGCTTTCGCGACCTCACGCTGGGAAACAGG‑CTCGCCGCGTCTGGAACGCTATAACGGTTATTCTGCGG‑TTGAGATTGTTGGGGAAGCCGCACCGGGGGTCAGTACCGGTACGGCGATGGATATTATGGAATCGTTAGTGAAGCAGCTGCCAAACGGCTTTGGTCTGGAGTGGACGGCGATGTCGTATCAGGAGCGGCTTTCCGGCGCGCAGGCTCCGGCGCTGTACGCCATTTCCTTGCTGGTGGTATTCCTGTGTCTGGCTGCGTTGTATGAAAGCTGGTCGGTGCCGTTCTCGGTA > NC_000913/2589833‑2590291 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |