Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,373,350 | A→G | 17.9% | L76P (CTC→CCC) | nanA ← | N‑acetylneuraminate lyase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,373,350 | 0 | A | G | 17.9% | 206.0 / 5.4 | 100 | L76P (CTC→CCC) | nanA | N‑acetylneuraminate lyase |
Reads supporting (aligned to +/- strand): ref base A (23/54); new base G (16/4); total (41/59) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.17e-05 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAA > NC_000913/3373116‑3373579 | gtgtTGAACTGAGCCAGGGGCAGTTTGACCCCGCGCAGGGCTGGAATGTTGTCCACCACCAACGGCAAACCATCCGGCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑Gt < 1:53140/237‑1 (MQ=255) gggtTGATCTAATCCAGGGTCAGGTTTACCCCACGCAGGGCTGGAATGTTGTACACCACCAACGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑Gtt < 1:46018/236‑1 (MQ=255) tgtTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCGACCGCCAACCA‑CGTGGGGGATGG‑GGTTa > 1:136380/1‑239 (MQ=255) ttGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGTTGACCGAACCGA‑CGCGGGCGGTAG‑GTTTA‑At > 1:434276/1‑239 (MQ=255) ttGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCTGACGCAACCCA‑CAGGGGCGATGG‑GGTTA‑At > 1:56444/1‑239 (MQ=255) ttGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATAGAAGCCATAACGTTTAGCCGATGCCGCACGTTGTTGGCTTTCGGCGGTGCTGACGAAACCGA‑AGTGGGGGATGG‑GTTTA‑At > 1:496477/1‑239 (MQ=255) atcCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCACGTTGTTGGCTTTCGGCGGGGCTGACGCAACCGC‑CGTGGGCGGTGA‑GGTTA‑ATCTTACCtt > 1:626904/1‑239 (MQ=255) atcCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGGTGCGACAACCCA‑CGGGGGGGAGGG‑GTTTA‑AATTAACCtt > 2:488698/1‑239 (MQ=255) ggTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCGTACCCAACCCG‑CGGGGGGGGAGG‑GTTTA‑ATTTTACCttttccc > 2:69700/1‑234 (MQ=255) aGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCCTCGAAGCCATAACGATTAGCCGATGCCGCAGGTTGTTGGCTTTCGGCGGTGCTGTGCCCACCGA‑CGTGGGCGATGC‑GTTTA‑ATTTTCACTTTTG‑CCTCTTc > 2:95398/1‑239 (MQ=255) aGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAGGTTGTTGGCTTTCGGCGGGGCGTACGCAACCGA‑CCGGGGCGATGG‑GTTTA‑ATTTTTCCTTTCG‑cgcctcc > 2:586864/1‑233 (MQ=255) aGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGGCGCAACCGC‑CGTGGGTGATGA‑GGTTA‑AATTTACCTTTCG‑CCTCtt > 1:96047/1‑238 (MQ=255) aGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCGGGCGCAACCCA‑CGTGGGCGATGA‑GGTTA‑ATTTTACCTTTGC‑CCTCTTc > 2:207950/1‑239 (MQ=255) gTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAACGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCGGAGGCACACCA‑CACGGGCGCAGG‑GGTTT‑ATATTTCCTTTCT‑CCCCTTCg > 2:677546/1‑239 (MQ=255) gTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGG‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCg > 1:714240/1‑239 (MQ=255) gTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCTGAGTCAACCAC‑CTGGGGCGATGA‑GGTTA‑ATCTTTACTTTCG‑CCCTTTCg > 1:472201/1‑239 (MQ=255) cTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACTGCATCGAAGCCATAACGTTTATCCGCTGCCGCAAGATGTTGGCTTTCGGCGGGGCGTGCGCCACCAA‑CGATGGCGATTAGGTTTA‑ACGTTACC‑TTCG‑CCTCTTCCGCGGCGG‑ttt > 2:526028/1‑238 (MQ=255) cTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGTCGGTGCTGACGCAACCGC‑CGTGGGCGAGGA‑GTTTA‑ATCTTACCTTTTG‑CCTCTTCTGGGCCGA‑ttt > 1:86027/1‑238 (MQ=255) cTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGCCGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGGGACGA‑ttt > 1:546053/1‑238 (MQ=255) cTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGACCGTGTGC‑ATGG‑GTTTA‑AACTTACCTTTTC‑CCCCTTCGGCGGCGA‑ttt > 1:24793/1‑238 (MQ=255) cTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CCTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑ttt > 1:466058/1‑238 (MQ=255) ggCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGATTCAATAATTGCCCGCTAGTGATCGCAGTGT‑TCTTCAAAGCTGATAGGATAGTAGAACGGCGTGACGGCGGAGACGACATCGAAGCCCTAACCTTTAGCCGCTGCCGCAAGTTTTTGGCTTTCGGCGGCGCAAACGACACCGA‑CATGGGGGATGT‑GGTAA‑ATATTTCCTTTCG‑CCTCTTCGGCGAGGA‑GTT‑cacaaa > 2:630886/1‑234 (MQ=255) cTGGAATGTTGTCACCCACCGTCGGCAAACCATCGCCCGAATCAATATTTGCCCGATCGGGATCGGAGTGT‑TCTTCAAAGCTGAAAGGATAGTAAAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTTTTGGCTTGCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTAc < 1:224000/238‑1 (MQ=255) tGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCGGCCGCAACCCC‑CGTGGGGGAGGA‑GTTTA‑ATTTTACCTTTTG‑CCCCTTCGGGCACGA‑ttttccagaccc > 2:181298/1‑230 (MQ=255) gAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCTGACGCAACCGC‑CGTGGGCGATGG‑ATTTA‑ATTTTACCTTTCT‑CCTCTTCGGCGACGA‑TTT‑cacgaaccttt > 1:18837/1‑229 (MQ=255) ttgtACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGTCGTGACGGCGGAGACGGCATCGAAGCCATAACGGTTCGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGGTGCACCAACCGA‑CAAGGGGGATCG‑GTGTACACTTTACCTTTC‑‑CCACTTCGGCGCCCA‑TTT‑TCACTaaccggtagc > 2:597620/1‑229 (MQ=255) ttgtACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACCCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTTAc > 1:67502/1‑238 (MQ=255) ttgtACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTCGTGGCGGAACCCA‑CGTGGGGGATGA‑GGTTA‑ATTTTCCCTTTTGCCCTCTCCGGCG‑CGA‑TTT‑GCAGTGCCTGtttaa > 2:743489/1‑235 (MQ=255) cccccaTGGGAAAACCCTCCCCCGAATCAATAATTGCCGCATAGTGATCGAAGGTT‑TTTTAAAACCTGAAAGGATAGTAGAACGGCGTGACGGGGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGaa < 2:804973/236‑1 (MQ=255) cccccaTCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGaa < 1:744358/236‑1 (MQ=255) cccccaACGGCAAACCATCCGCCAAATCAAGAATTGCCCGATAGTGAACGCAGGGT‑TCTTCAAAGCTGAAAGGGTAGTAGAACGGCGGGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGaa < 1:834581/236‑1 (MQ=255) caccaTCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGGGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGaaa > 1:65267/1‑239 (MQ=255) caccaTCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAATGGTTGGCTTTCGGCGGTGCTGAAGCCACCAC‑CGTGGGGGGTGA‑GTTTA‑ATCTTTCTTTTCC‑CCCTTTCGG‑GTCGAGTTT‑CCAAAACACGGTCACGCTCGGaaa > 2:375582/1‑239 (MQ=255) ctccgCAAACCCACCCCCGAATAAATAATTGCCCGATAGTGATCGGAGTTT‑TCTTAAAAGGTGAAAGGATAGTAGAACGGCGGGCCGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCt < 2:714240/235‑1 (MQ=255) tCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTTT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCtt < 1:444327/239‑1 (MQ=255) tCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCtt < 1:69700/239‑1 (MQ=255) ggCAACCCATCCGCCGAATTAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCt < 1:692165/236‑1 (MQ=255) aCCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGG‑GGTTA‑ATCTTTCCTTTCG‑CCTCTTCGGCGAAGC‑TTT‑CCAGTACCCGTTTACGCCCGGAAAGGCTTTTTTCaa > 1:92589/1‑239 (MQ=255) ccgAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCg < 1:207950/239‑1 (MQ=255) caaataaataTTTGCCCGTTAGTTATGGCAGTTT‑TTTTCAAGGGTGAAAGGATGGTAGCAGGGCGGGACGGGGGGGCAGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGc < 2:18837/237‑1 (MQ=255) aaTCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGGGCTTACGCCACCCA‑CGTGGGCCGTGG‑GTTTA‑ATCTTACCTTTTG‑CCTCCTCCGCGCCGG‑TTTCCCAGAACCT‑CTAACGCCCGCAAAGGCTTTTTCCAAAAGcccccccc > 2:100020/1‑233 (MQ=255) aatTTCCCATTAGTGGTCGC‑GTGTGTCTTAAAAGTTGAAAGGGTAGTAGAAGGGCGGTACGGCGGAGACGGAATCGGAGCCATAACGGTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTAACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCCCGCCGGTCGAac < 2:540921/239‑1 (MQ=255) ttGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAacca < 1:607518/239‑1 (MQ=255) gCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGC‑CGTGGGCGATGG‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCCGTACCTGTTCACGCCCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAaccac > 1:673047/1‑238 (MQ=255) gCCCGATAGTGATCGCAGGTT‑TCTTAAAAGCGGAAAGGTTAGTAGAACGGGGTGACGGCGGAGACGGGATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAaccacc < 1:612504/239‑1 (MQ=255) gCCCGATAGTGAGCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAaccacc < 1:208906/239‑1 (MQ=255) gCCCGAGAGTGAACGCCGTTT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAaccacc < 1:705285/239‑1 (MQ=255) cccGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGGCGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTTCGCGACGA‑TTT‑CCAGTACCTGTTTACGCTCGGAAAGGCCTTTTGCAAAAGCCCTGCCggcgggaacccc > 1:293342/1‑228 (MQ=255) cccGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCCA‑CGGGGGCGATGA‑GGTTA‑ATCTTCCCTTTCG‑CCCCTTCGCCGCCGA‑TTT‑CCCGTACCCTTTCAACCCCGGAAAAGCTTTTGAAAAAGGCCTCCCCCGGGGacccaccc > 2:56058/1‑239 (MQ=255) gacaggtggaccGCAGGGT‑TCTTAAAAGTTAAAAGGAGAGTAGAAGGGGGTGACGGGGGAGACGGCATGGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAaccacc < 1:320836/226‑1 (MQ=255) aTAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTTACGCTCGGAAAGGCTTTGTACAAAGGCCTCCCCGGTCGAACCACCCCCgc > 1:145886/1‑238 (MQ=255) gATCGGAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATaaa < 1:885530/239‑1 (MQ=255) gcgcggtggt‑TTTTCAAAGCTAAAAGGTTAGTAGAACGGCGGGGCGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAAc < 2:92589/231‑1 (MQ=255) accGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATaaa < 1:686138/236‑1 (MQ=255) t‑tcttcAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCt > 1:644338/1‑134 (MQ=255) t‑tcttcAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCt < 2:644338/134‑1 (MQ=255) gttttccAAAGAGGAAAGGTTATTAGGACGGGGTGGCGGGGGAGACGGCACCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTTTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATg < 2:181611/233‑1 (MQ=255) tcttcAAAGCTGAAAGGATAGTAGAACGGCGTGCCGGCGGAGACGGCCTCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGcc < 1:488698/239‑1 (MQ=255) tcttcAAAGCTGAAAGGATAGTAGAACGGCGTGACGGGGGAGACGGCAGCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGcc < 1:518501/239‑1 (MQ=255) cAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTT‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGGCGAACCACCCCCGTATAAACCGCCGATGCCctgc > 1:522729/1‑239 (MQ=255) aaaaGCGGAAAGGATATTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCctgc < 1:56058/238‑1 (MQ=255) aaaGGTTAAAGGATGAGAAAAGGGCTTGTCGGAGGAAGCGGCCGCGAAGCCATAACGGTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGCCCCAACCGA‑TGTTGGCGGTGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCctgct < 2:595882/239‑1 (MQ=255) aaaGCTGAAAGGATTGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCctgct < 1:586864/239‑1 (MQ=255) aaaGCTGAAAGGATAGTAGAACGGGGTGACGGCGGGGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCctgct < 1:396617/239‑1 (MQ=255) aaaGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCctgct < 1:887813/239‑1 (MQ=255) aaaGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCctgct < 1:544866/239‑1 (MQ=255) aaaGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCctgct < 1:596406/239‑1 (MQ=255) aGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGGAGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGGTCTCGCTCGGAAAGGCCTTTTTCAAAAGCCCCCCCGGTCGACACACCCCCGGAAAAAACCCCGATGCCCCGCTGa > 2:483000/1‑239 (MQ=255) agtagAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGGGATGA‑GTTTA‑ATCTTCCCTTTCG‑CCTCTTCCCCGAGGA‑TTT‑CCCGTACCTGTTCCCGCCCCGGAAGGGTTTTTTCAAAGGCCCCCCCCGGCGAACCCCCCCCCCAAAAACCGTCCAAGCCCCTCTGaaaaataaccaaa > 2:610319/1‑228 (MQ=255) cGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGG‑GTTTA‑ATCTTACCTTTTG‑CCTCTTCGGGGCCCG‑TTT‑CCAGTACCTGTTCCCGCTCGGGAAGGCTTTTTACAAAGGACTCGCCGGTCGGACCCACCCCGTATAAACCGCCGAGGCCCTGCCGAAAATTGAACAAAAACAGGGGAc > 2:234979/1‑239 (MQ=255) tGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACg < 1:69779/238‑1 (MQ=255) ggcggAGCCGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGAc < 1:332560/239‑1 (MQ=255) gcggGGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGAc < 1:640743/238‑1 (MQ=255) gaCGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCt < 1:181298/238‑1 (MQ=255) gaCGGCATCGAAGCCATAACGTTGAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCt < 1:526028/238‑1 (MQ=255) aTCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTTTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCa < 1:787057/239‑1 (MQ=255) aTCGAACCCATAACTTTTAGCCGGTGCCGCAAGTTTTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCa < 1:674619/239‑1 (MQ=255) aaCGTTTCGCTGAGGCCGTAATTTGTTGGGTTTCGGCGGTGATGGCGTAACCGA‑CGGTGGCGATGA‑GTTTA‑ATCTTAACTTTCG‑CCTTTTCGGCGACGA‑TTT‑CCAGTACATGTTCACGCTTGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCttgttgt < 2:323486/239‑1 (MQ=255) aaCGTTTAGCCGATGCCGCAAGTTGTTGGTTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTGCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCttgttgt < 1:24223/239‑1 (MQ=255) gggcGATTCCGCAGGTTGTGGGCTTTGGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTTCCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCaa < 2:466058/236‑1 (MQ=255) cctgtGCCGCAAGTTTTTGGCTTTCGGGGGTTCTGCCGCAACCGG‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTGCCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCaaaa < 2:558242/235‑1 (MQ=255) aTGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTACTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGGGCTTGTTGTTGGTCAAAAGGa > 1:145069/1‑239 (MQ=255) gCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCCCGGAAAGGCTTTGTACCAAGGCCTCGCCGGTGGAACCCCCCACGTATAAACCGTCGATGCCCTGCTGAATATTTAACTGAAACAGGCGACGCAGACCCCCCTTTTCCCGTGCTTTTTTTTGGTCcaaaaaagg > 2:633960/1‑234 (MQ=255) gcggtttgttgGTTTTCGGGGGTGCTTGCGCAACCGC‑GGTGGGCGATTA‑GTTTA‑AGCTTACCTTTCG‑CTTCTTCGGCGCAGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGACAg < 2:185154/234‑1 (MQ=255) ggttgGCTTCGGGCTGTGCTGGCGGAACCGA‑CGTGGGCGATGA‑GGTTA‑ATCCTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CAAGTCCCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGc < 1:434053/238‑1 (MQ=255) gttgGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCa < 1:787815/239‑1 (MQ=255) cgggggTGTTGGCGCACCCGG‑TGGGGGCGGTGA‑GTTTA‑ACCTTTCCTTTCG‑CCTTTTCGGCGACGA‑TTT‑CCAGTACCCGTTCCCGCCCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACg < 2:145886/239‑1 (MQ=255) cggcggTGCCGACCCAACCGG‑GCGGGGGGTTTG‑GTTTA‑ACCTTACCTTTCG‑CCTTTTCGGCGACGA‑TTT‑CCAGTTCCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACg < 2:170807/239‑1 (MQ=255) ccacccGA‑CGGGGGCGGTGG‑ATTTA‑ATTTTCCCTTTCG‑CCCTTTCGGGGGCGA‑TTC‑CCAGTACCTGTTCACGCCCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCg < 2:67502/235‑1 (MQ=255) aaCCGA‑CGTGGGCGTTTG‑TTTTA‑ACCTTTCCTTTCC‑CCTCTTCGGCGCCGA‑TTC‑CCGGACCCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGtt < 2:65267/239‑1 (MQ=255) tggCGATGA‑GTTTA‑ATCTTGCCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CGAGTACCTATTCACGCTCGGAAAGGCTTTGTACAAAGGCCGCGGCGGTCGAACCACCCACGTATAAACCGTCGATGGCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGCCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa < 1:746759/237‑1 (MQ=255) gggCGTTGT‑GTTTT‑ATTCTACCTTTCG‑CCTCTTCGGGGACGA‑GTT‑CCCTGTCCGGTTCCCCGCGGGAAAGGCTTTCTACAAGGGCCTCGCCGGGCGAACCACCCCCGTATAAACCGGCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCTACTACGCCACGTAAATTCGTTGCCATaaa < 2:142757/238‑1 (MQ=255) gggCGATTA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCCACGA‑TTT‑CCAGTCCCTGTTCCCGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa < 1:814534/238‑1 (MQ=255) gggCGATGG‑TTTTA‑ATTTTTCCTTTCG‑CCTTTTCGGCGACGA‑TTT‑CCAGTACCTGTTCCCGCCCGGAAAAGCTTTTTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa < 2:626904/238‑1 (MQ=255) gggCGATGA‑TTTTA‑ATCTTTCCTTTCG‑CCTCTTCGGCGTCGA‑TTT‑CCAGTCCCTTTTCACGATCGGAACGGCTTTGTCCAAAGGCCTCGACGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa < 1:489400/238‑1 (MQ=255) gggCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa < 1:483000/238‑1 (MQ=255) gggCGATGA‑GTTTA‑ATCTTACCTTGCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa < 1:637991/238‑1 (MQ=255) gggCGAAGA‑GTTTA‑ATCTTACCTTTCG‑CCTCGTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCCCGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGACCCAGTCGACGCATACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATaaa < 1:500635/238‑1 (MQ=255) ggCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGcc > 1:454709/1‑123 (MQ=255) ggCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGcc < 2:454709/123‑1 (MQ=255) | GTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGT‑TCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGA‑CGTGGGCGATGA‑GTTTA‑ATCTTACCTTTCG‑CCTCTTCGGCGACGA‑TTT‑CCAGTACCTGTTCACGCTCGGAAAGGCTTTGTACAAAGGCCTCGCCGGTCGAACCACCCACGTATAAACCGTCGATGCCCTGCTGAATATTGAACTGAACCAGGCGACGCAGACTCGCTTTATCCAGTGCTTGTTGTTGGTCAAAAGGAGTCAGGAGTGCAGCCATTACGCCACGTAAATTCGTTGCCATAAA > NC_000913/3373116‑3373579 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |