Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 4,146,772 | T→G | 14.5% | V172G (GTG→GGG) | pflC → | putative [formate‑C‑acetyltransferase 2]‑activating enzyme; pyruvate formate‑lyase 1‑activating enzyme |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,146,772 | 0 | T | G | 14.5% | 175.5 / 2.6 | 79 | V172G (GTG→GGG) | pflC | putative [formate‑C‑acetyltransferase 2]‑activating enzyme; pyruvate formate‑lyase 1‑activating enzyme |
Reads supporting (aligned to +/- strand): ref base T (17/46); new base G (7/7); total (26/53) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.16e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCG > NC_000913/4146547‑4147008 | atatCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑GAACGCCCCAGCCACGAAACAATTACCGCTGGCGAAATTGGTGGAT‑GAAG‑GGTTGTGCGGTTTa > 2:750082/1‑239 (MQ=255) aGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATGTTGCGAT‑GAAG‑GGTTGTTCGGTTTACAAAt > 1:170274/1‑239 (MQ=255) gACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGACGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCCACTGCGGCTGTGGGGTGGGT‑CATGCGCCCTTGAAGATGCCGG‑AGACGCACCAGCCAGCAAGCTCTTACCGCGGGCGGAATTGGAGGAT‑GAGG‑AGTTTTTCTGTTTAAAAAtatagg > 2:188164/1‑233 (MQ=255) gACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATTGGCCATTGAACCTGCCGG‑AGCAGCCCCAGCCAGCCAGCTATTACCGCTGGCGAAATTG‑GCGGC‑GTAGAGGTTGTTCGATTTTAAAATTATGGa > 2:208211/1‑239 (MQ=255) gACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑GGTTGTTCGATTTAAAAATTATgg > 1:703293/1‑238 (MQ=255) ctGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑GGTTGTTCGATTTAAAAATTATGGACGCGa > 1:560135/1‑238 (MQ=255) tgaaaCGGGAAGTTATGAAATGTGACATTTTTTGTCGCACGTCCGGGGGCGGCGCTCCGCTTTCTGGCGGGGAAGTGTGAATGCAGGCGGAGGTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACt < 2:170274/236‑1 (MQ=255) tGGAACGGGAAGTGATGAAAGATGACATTTTCTTGCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACt < 1:790881/239‑1 (MQ=255) cGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGgc < 1:684591/239‑1 (MQ=255) cGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGgc > 1:367691/1‑239 (MQ=255) gggAAGTGAGGAAAGAGTGCTTTTTCTTTCGCCTGGTCGGGGGCGGCGTCCCGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGAACGCGACTCAGgcg < 2:11653/239‑1 (MQ=255) ggAAGGGATGAAAGATTCCATTTTCTTTCGCACGTGTGGCGGCGGGGTCCCGTTTGCTGGCGGCGAAGTGTTAAGGCAGGCGGAGTTTGCTACCCGTTTTTTGCAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGgcg < 2:486976/238‑1 (MQ=255) gAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGAGGCCCCAGCCAGCAAGCTATTACCGCTGGCGAAATTGGGGGGT‑GAAA‑GGGTTTTGGATTTAAAAAATAAGGAGGCGACTCAGgcggg > 2:366601/1‑237 (MQ=255) gAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTTCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACCCGACTCAGgcgag > 1:420453/1‑237 (MQ=255) gAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACCCGACTCAGGcgcg > 1:621644/1‑239 (MQ=255) ggtatgaAAGATGCCATTTTCTTTCGCACGTCCGGCTGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGATTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCggg < 1:731875/235‑1 (MQ=255) tgtgaAAGTTGGCGTTTTCTTTCGCACGTCCGGCGGGGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAt < 2:560135/236‑1 (MQ=255) gattaAAGATGACATTTTCTTTCGCACGGCCGGCGGCGGCGTCCCGTTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGa < 2:388535/237‑1 (MQ=255) gatgaAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAt < 2:136468/238‑1 (MQ=255) gatgaAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATCACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAt < 2:322846/238‑1 (MQ=255) gACTTTTTTTTGCCCCCGCCCGGCGGGGGCGTACCGCTTGCTGGGGGGGAAGTGTTAAGGCAGGCGGTGTTTGCTCCCCTTTTTTTACAGCGACGCGGGCTGTGGGGT‑TGTCCATGCGCCATTGAACCTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGa < 2:118322/238‑1 (MQ=255) tcGCGCGTCCGGGGGCGGCGTCCCGCTTTCTGGGGGCGAAGTGTTAATGCAGGCGGAGTTTGCTCCCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCAc < 2:703293/239‑1 (MQ=255) ccgcgcccGGGGGCGGGGTACCGTTTTCTGGCGGGGAAGTGTTAATGCGGGGGGAGTTTGCTACCCGTTTTTTACAGCGCCTGCGGCTGTGGGGGTTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCAcgc < 2:891741/233‑1 (MQ=255) ccGGGGGGGGCGTCCAGTTTTCGGGCGGGGAAGTGTTAAGGCAGGGGGAGTTTGGGACCCGTTTTTTACAGCGCCGGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAGACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTg < 2:615432/239‑1 (MQ=255) cgctgtcctctttttTGGCGGGGAAGTGTTAATGCGGGCGGTTGTTGCCACCCGTTTTTGACAGGGTCTGCGGCTTGGGGGTGTGT‑AATGGGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCCTTTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAAt < 2:84867/227‑1 (MQ=255) gttgtgttttCTGGGGGAGAAGTGTTAATGGCGGGGGAGTTGGCTCCCCGTTTTTTACGGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCAGTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGc < 2:127141/231‑1 (MQ=255) tCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑GGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGAGGTGGGGAAAATGAACCTGCCACGCGTGCTGGGGAATCTGCGt > 1:646532/1‑239 (MQ=255) ttttgtgcggggAAGTGTTAATGCAGGGGGGTTTTGCTTCCCGTTTTTGAACGGGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTg < 2:88767/233‑1 (MQ=255) tttCTGGGGGCGAAGTGTTTATGCAGGGGGGGTTGTCTACCCGTTTTTTACAGCGACTGCGGCTGTGTGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCAGCAGCCAGCAAGCTGTTACCGCTGGCGAAATGGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCGACGCGTGCTGGAGAATCTGCGTTTGCTg < 2:203720/239‑1 (MQ=255) ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑GGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGGT‑GACG‑AGTTGTCTGATTTAAAACTTATTGACGCGATCCAGCCGCGGAGCGTGGTGGAAGTGAACCTGTCACGAGTGCCGTCGGATCTGGGTTTGGTGGTGGGTGGGGGGGGCAACGGGAccc > 2:669373/1‑239 (MQ=255) ggCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATc > 1:573226/1‑238 (MQ=255) cGGAGTTTGCTACCCTTTTTTTACAGCGACTGCGGCTGGGGGGTTGGT‑CATGCGCCATTGAAACTGCCGG‑GGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TTTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATccc < 1:852165/238‑1 (MQ=255) tttGCTCCCCTTTTTTTCCAGCGCCTGCGGTTGGGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑CGACGCCCCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGt‑t < 2:367691/238‑1 (MQ=255) tttGCTACCCGTTTTTTAACGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACGGCCGG‑AGACTCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGATTCAGGCGCGGGATGTAGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGt‑t < 1:57325/238‑1 (MQ=255) tGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTAc < 1:695877/239‑1 (MQ=255) gTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGGGCTGGAGAATCTGCGTTTGTTGGGGGGTGAGGGCGTCAACGAGAACCC‑CGTGTTACCCCTGAt > 2:719276/1‑238 (MQ=255) gCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCCGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGGGTTTTCTGGGGAGTGAGGGCGTCAACGGGATCCCGCGT‑TTACCGCGCACCCCTGGGTTc > 2:411548/1‑238 (MQ=255) gCTGGGGGGTTGGT‑CATGCGCCATTGAAACGGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGc < 1:45214/238‑1 (MQ=255) ggggggTGT‑CAGGGGCCATTAAAACGGCCGG‑GGACGCCCCAGCCAGCAAGCTATTACCGGTGGCGAAATTGTGGGAT‑GAAT‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGga < 2:591253/238‑1 (MQ=255) gtgtct‑CATGCGCACTTGAAACTGCCGG‑AGACGCCCCAGCCAGCAAACTATTCCCGCTGGCGAAATTGTGCGAT‑GAAG‑GTTTGTTCTATTTAAAAAGTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTTGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGaa < 1:799688/238‑1 (MQ=255) tgt‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGGGAATCTGAGTTTACTGGTGGGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTGACGTCCGGCCGGGAGaaaag > 2:469679/1‑235 (MQ=255) tgt‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGGGAGTGAGGGCGTCAACGGGATCCCGCGT‑TTACCGCTGATCCCTGGTTTAACCCCCAGCCggggaaaaat > 2:325910/1‑231 (MQ=255) tgt‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCCGGCAAATCTGCGTTTGCTGGGGAGGGGGGGCGCCAACTGGGTCCGGCGT‑TTACCGCCTAACCCTGGTTTTACCCCCGCCCGGGGGaaaaac > 2:460611/1‑235 (MQ=255) gttttgagcgcCGTTTAATCTTGCGG‑AGACGCCCCCACCAGAAAGGTTTTACCGTTGGCGAAATTGTGCTAT‑TAAG‑TTTTTTGCGATTTAAAAATTCTGGACGCTACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGGTCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAatat < 2:466477/232‑1 (MQ=255) cATGCCCCATTGAAATTGCCGG‑GGGAGGACCGCGCCGCAACATATTACCGGTGGGGAAATTTGGGGAG‑GAAG‑GTTTGTGTGATTTAAAAATTGTGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATgcag < 2:878296/239‑1 (MQ=255) cgccgcCATGGAACCTGCCGG‑GGACGCCCCAGACAGCAAGCTTTTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGCCGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATgcagc < 2:646532/236‑1 (MQ=255) aTGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATgcagc < 1:366601/239‑1 (MQ=255) tggcccATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATgcagca < 1:719206/235‑1 (MQ=255) tGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGGGGCACCGGGATCCCGCGT‑TTACCGCTGCTCCCTGGTTTACCGCCCACCCGGGGGAAAATgcaaag > 2:800344/1‑236 (MQ=255) ccATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTTGCGAAATTGGGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgc < 1:404132/238‑1 (MQ=255) taTTGAAACTGCCGT‑AGACCGCCCGACAAGCAAGCTGTTACCGGTGGGGAAATTGTGGGAT‑GAAG‑GGTTGTGTGATTTAAAAATTATGGACGCGATTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGTGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgcgc < 2:323172/238‑1 (MQ=255) cATTGAAACTGCCGG‑ATACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑GGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgcgc < 1:526671/239‑1 (MQ=255) cATTGAAACTGCCGG‑AGACGCACCAGCCAGTAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgcg < 1:886691/238‑1 (MQ=255) aaTTGAAACTGCCGG‑AGACGAACCAAGCAGCAAGATATTACCGGTGGCGAAAGTGGGCGAT‑GAAG‑GGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGGGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGgcgc < 1:836698/238‑1 (MQ=255) ttGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGt > 2:139448/1‑169 (MQ=255) ttGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGt < 1:139448/169‑1 (MQ=255) ttGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGGGAATATGCAGCAGCAGCTg > 1:730580/1‑239 (MQ=255) tGAAACCGCCGG‑GGACGACACAGCCAGCAAGCTATTACCGCTGGCTAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTgg < 1:669373/239‑1 (MQ=255) ggAAAGTGCCGGAAGACACA‑CAGCCAGCAAGGTATTACGGTTGGCGAAATTGGGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTAGGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTgg < 2:621644/238‑1 (MQ=255) gAAACTGCCGG‑GAACGCACAGGCCAGCAAGCTATTCCGGTTGGTGAAATTGTG‑GATAGAAT‑GGTGGTTCGATTTAAACATTAGGGAGCCGACTCAGGCGCGGGATTGGGTGAAGAGGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGa < 2:710769/239‑1 (MQ=255) gCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCACCAGGCGCTGGATGt > 1:161466/1‑236 (MQ=255) gCCGG‑AGACGCACCAGCAAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGATTGATGGTGAGGGAGGGCGTCAACTGGATCCCGCGT‑TTACCGCGGATCTCTGGTTTAACGCTCAGACGGGGGAATAGGCAGCAGGCGCTGGagggacg > 1:141676/1‑233 (MQ=255) cgccagaAAGCTTTTACCGCTGGGTAAATTGTGGGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAata < 2:573226/238‑1 (MQ=255) cagaAAGCTATTACCGCTGGCAAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGAAGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTTAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAata < 1:188164/236‑1 (MQ=255) catgCTTTTTCCGCGGGCGAAATTGTGGGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGGCTCAGGCGGGGGTTGGGGTGAAGATGAACCTGCCCCGGGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGc < 2:821613/236‑1 (MQ=255) gagttATTTCCGCTGGCGAAATTGGGCGAT‑GAAG‑TGTTGGTCGATTTAAAAATGACGGACGGGACTCCGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGc < 1:148134/234‑1 (MQ=255) aaGCTATTACCGGTGGCGAAATTGGTCGAT‑GAAG‑TGTTGTTCGATTTAAAAAGTATGGCCGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCGCGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAgg < 1:821079/237‑1 (MQ=255) aaGCTATTACCGCTGGCGAAATTGTTCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGc < 1:213749/238‑1 (MQ=255) aaGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGc < 1:325910/238‑1 (MQ=255) gCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGGGAATATGCAGCAGGGGCTGGATGTACTGATCCCGCTGAATATCAGGCGGa > 1:113458/1‑239 (MQ=255) ttATTTCCGCTGGGGAAATTTGGGGAT‑GAAG‑TGGTGTTCATTTTAAAAATTATGGACGTGACTCAGGCGCGGGATGTGGTAAAGATTAACCGGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGGCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGAt < 2:127705/238‑1 (MQ=255) aTTAGCGGTGGCAAAATTGGTCGAT‑GAAT‑TGTTGGTCGATTTAAAAATTATGGACGGGAATCGGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATcc < 1:776447/239‑1 (MQ=255) gtGGCGAAATTGTGGGAG‑GAAG‑GGTTGGTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGtt < 1:337368/238‑1 (MQ=255) tGGCGAAATTGTGCGAT‑GAAG‑TGTCGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGtt > 1:694158/1‑238 (MQ=255) tGGCGAAATTGGGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGtt < 1:800344/238‑1 (MQ=255) ggCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTa > 1:482347/1‑238 (MQ=255) aaTTGTGCGAT‑TAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGttt < 1:460611/239‑1 (MQ=255) tgtgGGAG‑GAAT‑TTTTTTTCGATTTAAAAATTATGGGCGCGACTCAGGCGGGGGATGTGGGGAAGATTGACCTGACACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTtcat < 2:288957/239‑1 (MQ=255) aG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCg < 1:750082/239‑1 (MQ=255) | ATATCAGCCTTGACGCTCTGGAACGGGAAGTGATGAAAGATGACATTTTCTTTCGCACGTCCGGCGGCGGCGTCACGCTTTCTGGCGGCGAAGTGTTAATGCAGGCGGAGTTTGCTACCCGTTTTTTACAGCGACTGCGGCTGTGGGGTGTGT‑CATGCGCCATTGAAACTGCCGG‑AGACGCACCAGCCAGCAAGCTATTACCGCTGGCGAAATTGTGCGAT‑GAAG‑TGTTGTTCGATTTAAAAATTATGGACGCGACTCAGGCGCGGGATGTGGTGAAGATGAACCTGCCACGCGTGCTGGAGAATCTGCGTTTGCTGGTGAGTGAGGGCGTCAACGTGATCCCGCGT‑TTACCGCTGATCCCTGGTTTCACGCTCAGCCGGGAGAATATGCAGCAGGCGCTGGATGTACTGATCCCGCTGAATATCAGGCAGATCCATCTGTTACCGTTTCATCAGTACGGCG > NC_000913/4146547‑4147008 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |